LOCUS       sequence-35777-e        8800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral vector for expression of Reverse Tetracycline 
            transactivator 3G (rtTA3G)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8800)
  TITLE     pLenti CMV rtTA3G Blast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti CMV rtTA3G Blast (R980-M38-658)
FEATURES             Location/Qualifiers
     source          1..8800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             231..411
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1796..1912
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1926..1946
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2101..2480
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2481..2684
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2635..2655
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2681..2705
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2770..2794
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2797..3543
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    complement(3542..3566)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3586..4174
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3639..3659)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4057..4068)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4225..4245)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4230..4560
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4411..4546
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4608..4655
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4674..5072
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5324..5557
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5629..5750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5666..5685)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5720..5739
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5769..5904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5936..5955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5937..5955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5965..5982)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5965..5981)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5974..5996)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6126..6581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6213..6232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6423..6444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6610..6714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6715..6783,6784..7575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6933..6952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7746..8334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8235..8254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8488..8505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8622..8643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8658..8663,8664..8681,8682..8688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8696..8712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8701..8723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8720..8736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8720..8736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8755..8775
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8757..8775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacaataaac gggtctctct
      241 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      301 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      361 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
      421 gaacagggac ctgaaagcga aagggaaacc agagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcctca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg ttaactttta
     1801 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     1861 cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgtcca
     1921 gatcacaact ttgtatagaa aagttggcga tctatacatt gaatcaatat tggccattag
     1981 ccatattatt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     2041 tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt
     2101 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     2161 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     2221 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2281 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2341 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2401 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2461 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2521 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2581 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     2641 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     2701 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2761 ccagcctccg caactttgta caaaaaagtt ggcaccatga gccggctgga caagagcaaa
     2821 gtgatcaaca gcgccctgga actgctgaac ggcgtgggca tcgagggcct gaccacccgg
     2881 aagctggccc agaaactggg cgtggaacag cccaccctgt actggcacgt gaagaacaag
     2941 cgggccctgc tggacgccct gcccatcgag atgctggacc ggcaccacac ccacagctgc
     3001 cctctggaag gcgagagctg gcaggacttc ctgcggaaca acgccaagag ctacagatgc
     3061 gccctgctga gccaccggga cggcgccaaa gtgcacctgg gcaccagacc caccgagaag
     3121 cagtacgaga cactggaaaa ccagctggcc ttcctgtgcc agcagggctt cagcctggaa
     3181 aacgccctgt acgccctgag cgccgtgggc cacttcaccc tgggctgcgt gctggaagaa
     3241 caggaacacc aggtcgccaa agaggaaaga gagacaccca ccaccgacag catgcccccc
     3301 ctgctgaagc aggccatcga gctgttcgac agacagggcg ccgagcccgc cttcctgttc
     3361 ggcctggaac tgatcatctg cggcctcgag aagcagctga agtgcgagag cggcggaccc
     3421 accgacgccc tggacgactt cgacctggac atgctgcccg ccgatgccct ggatgatttt
     3481 gatctggata tgctccctgc tgacgctctc gatgacttcg atctcgatat gctgccaggc
     3541 taatcaactt tcttgtacaa agttgtgatg tggcggccgc tcgacaatca acctctggat
     3601 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3661 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3721 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3781 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3841 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3901 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3961 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     4021 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4081 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4141 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttctgcagat atccggttag
     4201 taatgagttt ggaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4261 gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4321 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4381 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4441 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     4501 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4561 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     4621 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     4681 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     4741 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
     4801 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
     4861 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
     4921 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
     4981 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
     5041 tgctgccctc tggttatgtg tgggagggct aagcacaatt cgagctcggc tgggtcgcgg
     5101 gagggccggt cgcagtgatt caggcccttc tggattgtgt tggtccccag ggcacgattg
     5161 tcatgcccac gcactcgggt gatctgactg atcccgcaga ttggagatcg ccgcccgtgc
     5221 ctgccgattg ggtgcagatc cgtcgagggc ccggtacctt taagaccaat gacttacaag
     5281 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac
     5341 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     5401 tgagcctggg agctctctgg ctgcctaggg aacccactgc ttaagcctca ataaagcttg
     5461 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5521 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     5581 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     5641 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5701 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5761 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5821 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     5881 agtgaggagg cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta
     5941 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6001 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6061 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6121 gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6541 atttaacgcg aattttaaca aaatattaac gtttacaatt tcccaggtgg cacttttcgg
     6601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     8641 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     8761 aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
