LOCUS       sequence-35778-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Cronin JC, Wunderlich J, Loftus SK, Prickett TD, Wei X, Ridd K, 
            Vemula S, Burrell AS, Agrawal NS, Lin JC, Banister CE, Buckhaults P,
            Rosenberg SA, Bastian BC, Pavan WJ, Samuels Y
  TITLE     Frequent mutations in the MITF pathway in melanoma.
  JOURNAL   Pigment Cell Melanoma Res. 2009 Aug . 22(4):435-44.
  PUBMED    19422606
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pigment 
            Cell Melanoma Res. 2009 Aug . 22(4):435-44."
COMMENT     Sequence Label: pCMV-Tag4A-MITF-M (E87R)
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..46)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     48..64
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     49..65
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttaattaagg naccgggccc tacttatcgt cgtcatcctt gtaatcctcg aggtcgacgg
       61 tatcgataag cttacaagtg tgctccgtct cttccatgct catactgctc ctccggctgc
      121 ttgttttgga agctccgggg gacactgagg aaaggagtgg atcagtgaca ccgacgggag
      181 aaagggtgtc gtccatcagg atgtcttcca gtttggatcc catttttgtg gggacactat
      241 aggcttggtt ggcctcagtc ccagttccga ggttgttgtt gaaggtgatg gtgccatccg
      301 tgagatcgag agttgttgta caggttaggt ctgcatgatg ctgaaggagg tcttggctgc
      361 agttctcaag aacgggttct tgcttgatga tccgattcac caaatctgga gagcagagac
      421 ccgtggatgg aataagggaa agtccatgag ctcgagcctg catttcaagt tcctgtattc
      481 tgagcaacaa atgccggttg gcgtgctcca gtttcttctg tcggttttca agttcttttg
      541 cgcgttgctn nnctcgttgc aactttcgga tatagtccac ggatgctttt aagatggttc
      601 ccttgttcca gcgcatgtct ggatcatttg acttgggaat caaagtacct agttctttaa
      661 tgcggtcatt tatgttaaat cttcttcttc gttcaatcag gttgtgattg tcctttttct
      721 gcctctcttt ggccagtgct cttgcttcag actctgtgag ctcccttttt atgttgggaa
      781 ggttggctgg acaggagttg ctgatggtga ggcctggtgg gggcaganct tggtttccat
      841 aaagatcaat caagtttccc gagacaggca acgtatttgc catttgc
//
