LOCUS       sequence-357788-        9161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2-F1103A-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN2-F1103A-dsRNAres_Y
FEATURES             Location/Qualifiers
     source          1..9161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6467..6508
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6518..6535
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6558..6575)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6608..6742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6632..6651)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6686..6705
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7343..7931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7423..7442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8102..8893,8894..8962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8725..8744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8963..9067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9135..9153
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttgaattctg cagatatcat ggactatgtc tactgtggaa cggatccgat
     2761 tggagctagc gaggatatcc tgtccgtgta cgatgccggc agtgcccccg gcaatggtca
     2821 tttcagcccg tcctttaacg gctttaatat tggaacgacg gaccccgagt acgtcgaact
     2881 ggtcccggtc ctggctgatg gaggtgaaca ttttggagtg agctccgtcg cctttgatga
     2941 ttatgaggaa ttgttgtgga tgggaaatca aggaggacat gtgaccagct attataccaa
     3001 tagcatgcaa aaatatacca gcttccaagt gcatgctacg gatatcgtcc gtgatattag
     3061 cacgttggat agcggcgtct tggccctgac ccaaacgagc ttgcgccacc aaattcgtcg
     3121 cggcttgccg aaattcacgt ttaagagcaa taatatgaaa gaaatggtct ccatgttgca
     3181 actgtcgccc caccgcttgg tgatggctgg cttgcaagat gaactgattg attttgattt
     3241 gcgtaccctg aaagagacgc gtattgaaca cgtcggcgct ggaggctgta ccgtcttgcg
     3301 taaaaactcg cgctacttgt tcgctggaga tcaattgggc accgttaccc tgcgtgattt
     3361 gaattccttg tccgtgcaac ataccattaa aacccatacg aatattctga gcgatttttc
     3421 cgtgcaaggc aacttgctga ttagttgtgg ctacagcgga aggcaaaata acctggccat
     3481 cgaccgcttc ctaatggtct acgatctgcg catgctgcgc ctgatcgcgc ccattcaagt
     3541 gatgattgat ccgcagatgt tgaagttcct gccatcgctg acatcccaac tggccgtcgt
     3601 gtccagctac ggccaggttc agctggtgga cactgtggag ctcagcgagc cgcgagtatc
     3661 catgtaccag atcaacacca acggcagcca gtgcctcagc ttcgacatta gctcctcttc
     3721 acaggccatg gcatttgggg accagtcggg ccacatcaac atgattgccg ctgtacaaac
     3781 gcctcagccg cagttcaatc tctactcgcg aagcacagag tttgcggacg tggtgccaca
     3841 gctgccgatg gtctccatta cggacaccaa ctttccgcta tcgtcggtca tgctaccgca
     3901 cttgacgacg ggcacacaat ggttctcaga ttggccggag gaactgctgc gctaccgcta
     3961 ccacagaccc aagaccattg atcccgaagt gctgagcaac atgaaaatgc agggaccgat
     4021 tggatactcg ccaaacccac gcaccgcccg tcgtaatcaa attccgtacg tgattgagca
     4081 gggcggcgtg tgcagtccga acggaaatgg aactgcggcg gcgaccaagg cggagaacgg
     4141 tgtgaaaatc attccacggc ggtatcgcaa agtcgagctg aagtacacga agctgggcac
     4201 ccaggacttt gactttgacc agcataatca aacatgtttc gcggggctgg aggcaacgct
     4261 accgaactcc tactgcaatg ctatgctaca gattttgtat ttcaccgacg cgcttcgcgt
     4321 gaagctgctg gagcattcgt gcatcaagga gttttgcctg tcctgcgaac tagggttcct
     4381 cttcaacatg ctggataaaa gtaccgcttc ttcaccttgc caggccagca atttcctgcg
     4441 atctttccgc actgtgccag aggcctcggc actgggattg attctcaccg accgctcatc
     4501 gaacgtcaat ctaatatcgc tgatacagaa ctggaaccga ttcattttgc atcaaatgca
     4561 ttacgaaata ttcgattcta gcaagaacgc atccacctac agcggatctg tgcaaacatc
     4621 aacgaacgcg gaaaacgcgg gctcttcaga aacaagtggc tcctcggacc tttacgattc
     4681 cataagtgat gagaactcca aggaggacga tcgggaacgc agtaagatca acgcagaaac
     4741 tgacatcagt aagatctttg ggaccaagca aatctgtata aaccgctgca tcaaatgcca
     4801 agaagagaag ataaaggaaa gcattctact tgcctgcaat ttgtcgtatc ccaaccacat
     4861 caaagatagt gatcagtatt tcaactttgg caccattttg aagcgatctc tcagctccga
     4921 gaagagtatt caggccttct gcgagagatg taagaagttt tcgccaacca atcagagcgt
     4981 gaaggtgact tcgcttccgc aaattctgtc cattaactgt ggcctcaaca acgaaaaaga
     5041 cattaccttc ctgaagcgcc agctcaatcg ctgcagcgaa aagacgactg ttgatgcggc
     5101 cgcttccttg agcacaagca agccgtgtcg atatggagcc aactgttcgc gaagtgactg
     5161 ccatttcatg catccggatc gaaaatctcc atcgcacaca agccaaccga atgctgtcaa
     5221 caattctccc aatggacgcc aaaagtcctg gtttcccctt acatttacta tgggcatcaa
     5281 tgaccaaggt gaggtgcagg tgcaaactca atcagatgca agcagtggca aatcggaaca
     5341 ggaggaggaa acggaaaagc caccaacaaa gggcttggac aacaatcgga tgtacgcgct
     5401 gcatgccgtg gtgtgccaag tcgatgatgg tacccaaaag aacttggtat cgttgataaa
     5461 tgtgcagcgg ccataccaca ccatgaaact ggctgaatct gcagatgacc ctcagagtca
     5521 atggtatatt tttaatgatt ttagcatatc gcccgtttcg ccacaagaat cggtatggtt
     5581 cacattggat tggaaagttc cttgcatact cttctacaga cacgtagagg atgactctga
     5641 gtctgctagt actactagct cgaccgtcac ggaaagtgaa gaaaccattc catcagagag
     5701 cagcagcggc tcgccaacaa atctatccaa cccttttcta gaggaaattg tgagtcctat
     5761 gctaggcaac ttaagtgcag acgccacgct gcagcccctg cagtcagatg aaatgccgca
     5821 gtcgggtgat ctggtcgcaa tggatgccga gttcgttacc ctaaatccag aggaaaatga
     5881 gattcgcccg gatggcaaaa cggcgaccat taagccatgc cacatgagtg tggcccggat
     5941 ttcatgtata cgaggacaag gcccggctga aggagttcca ttcatggacg attacatatc
     6001 cacccaggag aaggtcgtgg actacctaac tcaggcctcg ggaatcaagc ctggcgactt
     6061 ggacgctaac ttcagcaaaa agcgactgac tgccttgaag tactcatacc agaaattaaa
     6121 atacctcgtt gacgtaggag taatatttgt cggacatggt ctgaagaacg actttagggt
     6181 cataaacatc tatgtaccgt cggagcagat aatagatacg gtgcacctgt ttcacatgcc
     6241 acatcatcgc atggtttccc taagatttct agcctggcat ttcttgggta ctaagattca
     6301 gtccgagacg cacgactcca tcgaagatgc acggaccact ctgcagctct acaagcacta
     6361 tttgaagctg caggaggaga agaagtttgc caacgctctc aagaatcttt acgaacgcgg
     6421 aaagcagctg cagtggaagg ttccagagga ctgaccgcgg ttcgaaggta agcctatccc
     6481 taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt
     6541 ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct gatcagcctc
     6601 gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     6661 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     6721 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     6781 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     6841 ttatgatcag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     6901 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     6961 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     7021 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     7081 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     7141 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     7201 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     7261 ggcaggtaga tgacgaccat cagggacagc ttcaaggcca gcaaaaggcc aggaaccgta
     7321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7741 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8101 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8161 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8221 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8281 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8341 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8401 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8461 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8521 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8581 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8641 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8701 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8761 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8821 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8881 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8941 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9001 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9061 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     9121 cattaaccta taaaaatagg cgtatcacga ggccctttcg t
//
