LOCUS       sequence-357790-       15083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 module containing HSP21-nYFP HSP21-cYFP OEP7-mTRQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15083)
  AUTHORS   Velay F, Soula M, Mehrez M, Belbachir C, D'Alessandro S, Laloi C, 
            Crete P, Field B
  TITLE     MoBiFC: development of a modular bimolecular fluorescence 
            complementation toolkit for the analysis of chloroplast 
            protein-protein interactions.
  JOURNAL   Plant Methods. 2022 May 26;18(1):69. doi: 
            10.1186/s13007-022-00902-1.
  PUBMED    35619173
REFERENCE   2  (bases 1 to 15083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2022-05-26"; volume: "18(1):69. doi"; pages: " 
            10.1186/s13007-022-00902-1"
COMMENT     Sequence Label: pLBM0060
FEATURES             Location/Qualifiers
     source          1..15083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1011..1356
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      1978..2230
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3246..3591
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4646..4669
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5226..5400
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        6413..6758
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             7951..8040
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    8081..8255
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        9268..9613
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(9811..9813,9814..9816,9817..10530)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9856..9877)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      10543..10795
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10861..10885
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10973..11683
                     /label=oriV
                     /note="incP origin of replication"
     promoter        11704..11808
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11809..12603
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12774..13362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13263..13282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13698..14846
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14992..15016
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga ggaattccaa tcccacaaaa atctgagctt aacagcacag
       61 ttgctcctct cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt
      121 ataacggtcc acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg
      181 gcgttcccgg agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg
      241 acgcgtacac aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc
      301 aactcaagcc caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact
      361 ggctcacgct aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg
      421 ccccggagat tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag
      481 gtgaaggtga cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca
      541 gaaagaatgc tgacccacag atggttagag aggcctacgc agcaagtctc atcaagacga
      601 tctacccgag taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag
      661 tcaaaagatt caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca
      721 gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag
      781 agattggagt ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag
      841 attcaaatcg aggatctaac agaactcgcc gtcaagactg gcgaacagtt catacagagt
      901 cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg
      961 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
     1021 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     1081 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     1141 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
     1201 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
     1261 gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     1321 tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt
     1381 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc
     1441 gatacaatgg aacgagctat acaaggaaac gatgctaggg aacaagctta tggtgaacgt
     1501 tggaatggag gatcaggaag ttccacttct cccttcaaac ttcctgacga aagtccgagt
     1561 tggactgagt ggcggctaca taacgatgag actatttcga atcaagataa tccccttggt
     1621 ttcaaggaaa gctggggttt cgggaaagtt gtatttaaga gatatctcag atacgacggg
     1681 acggaaactt cactgcacag agtccttgga tcttggacgg gagattcggt taactatgca
     1741 gcatctcgat ttctcggttt cgaccagatc ggatgtacct atagtattcg gtttcgagga
     1801 gttagtgtca ccatttctgg agggtcgcga actcttcagc atctcagtga aatggcaatt
     1861 cggtctaagc aagaactgct acagcttacc ccagtcaaag tggaaagtga tgtatcaaga
     1921 ggatgccctg aaggtgttga aaccttcgaa gaagaaagcg agtaagcttg tcaagcagat
     1981 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     2041 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     2101 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     2161 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     2221 ttactagatc gacgctgcaa gaattcaagc ttggaggaat tccaatccca caaaaatctg
     2281 agcttaacag cacagttgct cctctcagag cagaatcggg tattcaacac cctcatatca
     2341 actactacgt tgtgtataac ggtccacatg ccggtatata cgatgactgg ggttgtacaa
     2401 aggcggcaac aaacggcgtt cccggagttg cacacaagaa atttgccact attacagagg
     2461 caagagcagc agctgacgcg tacacaacaa gtcagcaaac agacaggttg aacttcatcc
     2521 ccaaaggaga agctcaactc aagcccaaga gctttgctaa ggccctaaca agcccaccaa
     2581 agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc cagcagtgat ccagccccaa
     2641 aagagatctc ctttgccccg gagattacaa tggacgattt cctctatctt tacgatctag
     2701 gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac cactgataat gagaaggtta
     2761 gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc tacgcagcaa
     2821 gtctcatcaa gacgatctac ccgagtaaca atctccagga gatcaaatac cttcccaaga
     2881 aggttaaaga tgcagtcaaa agattcagga ctaattgcat caagaacaca gagaaagaca
     2941 tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg cttcataaac
     3001 caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcctactgaa tctaaggcca
     3061 tgcatggagt ctaagattca aatcgaggat ctaacagaac tcgccgtcaa gactggcgaa
     3121 cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt caacatggtg
     3181 gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga agatcaaagg
     3241 gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt ccattgccca
     3301 gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
     3361 cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg tcccaaagat
     3421 ggacccccac ccacgaggag catcgtggaa aaagaagagg ttccaaccac gtctacaaag
     3481 caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct
     3541 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctcgagt
     3601 ataagagctc atttttacaa caattaccaa caacaacaaa caacaaacaa cattacaatt
     3661 acatttacaa ttatcgatac aatggcttcc tctatgctct cttccgctac tatggttgcc
     3721 tctccggctc aggccactat ggtcgctcct ttcaacggac ttaagtcctc cgctgccttc
     3781 ccagccaccc gcaaggctaa caacgacatt acttccatca caagcaacgg cggaagagtt
     3841 aactgcatgc aggtcattta tatttcttct ttcacttttt tattattcca tatgattttt
     3901 ttcggttctt tcttcgaatc tacataaact aatatcattg gaaaaatcga aaaaataggt
     3961 gtggcctccg attggaaaga agaagtttga gactctctct taccttcctg accttaccga
     4021 ttccgaaggt gctcaagacc agagagaaaa ctccattgat gttgttcagc aaggacaaca
     4081 aaaagggaac caaggatcaa gtgtcgaaaa aagacctcaa caacgcttaa ccatggatgt
     4141 ctctcctttc ggattgttgg atcctttgtc accaatgagg acgatgcgac aaatgttaga
     4201 tactatggac aggatgttcg aggacactat gcctgtctca ggaagaaaca gaggaggaag
     4261 tggagtgtca gagattcgtg caccgtggga catcaaagag gaagaacacg agatcaagat
     4321 gcgtttcgac atgcctggac tctctaaaga ggacgtcaaa atctctgtag aagataacgt
     4381 acttgtgatc aaaggagagc agaagaagga ggacagtgat gattcttggt ctggaagaag
     4441 cgttagctca tatggaacac gacttcagct cccagacaac tgtgagaaag acaagatcaa
     4501 agctgagctc aagaacggag tcctctttat cactatccct aagaccaaag tcgaacgcaa
     4561 agtcatcgat gtccagattc aatcttcgga ttacaaggac cacgacgggg actacaagga
     4621 tcatgatttg gatgctgctg ccgccgacta taaggatgat gacgataagg tgagcaaggg
     4681 cgaagagttg ttcactggtg tggttcctat cctcgttgag cttgatggtg atgtgaacgg
     4741 gcacaagttc tccgtttctg gtgaaggtga gggtgatgct acttacggaa agctcaccct
     4801 caagttcatc tgtaccactg gaaagctccc tgtgccttgg cctactctcg ttactacttt
     4861 cggatacgga ctccagtgct tcgctagata ccctgatcat atgaagcagc acgacttctt
     4921 caagagcgct atgcctgagg gatacgtgca agagagaacc attttcttca aggacgacgg
     4981 caactacaag accagagctg aggttaagtt cgaaggtgac accctcgtga acaggatcga
     5041 gcttaagggc atcgacttca aagaggacgg aaacatcctc ggacacaagc tcgagtacaa
     5101 ctacaacagc cacaacgtgt acatcatggc cgacaagcag aagaacggca tcaaggtcaa
     5161 cttcaagatc aggcacaaca tcgaggacta agcttctcta gctagagtcg atcgacaagc
     5221 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
     5281 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
     5341 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
     5401 gctactagaa ttcgagctcg gaggaattcc aatcccacaa aaatctgagc ttaacagcac
     5461 agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact actacgttgt
     5521 gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg cggcaacaaa
     5581 cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa gagcagcagc
     5641 tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca aaggagaagc
     5701 tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc aaaaagccca
     5761 ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag agatctcctt
     5821 tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa ggaagttcga
     5881 aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc tcttcaattt
     5941 cagaaagaat gctgacccac agatggttag agaggcctac gcagcaagtc tcatcaagac
     6001 gatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg ttaaagatgc
     6061 agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat ttctcaagat
     6121 cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat
     6181 agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc atggagtcta
     6241 agattcaaat cgaggatcta acagaactcg ccgtcaagac tggcgaacag ttcatacaga
     6301 gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc
     6361 tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct attgagactt
     6421 ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
     6481 tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
     6541 gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga cccccaccca
     6601 cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa gtggattgat
     6661 gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
     6721 cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata agagctcatt
     6781 tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca tttacaatta
     6841 tcgatacaat ggcttcctct atgctctctt ccgctactat ggttgcctct ccggctcagg
     6901 ccactatggt cgctcctttc aacggactta agtcctccgc tgccttccca gccacccgca
     6961 aggctaacaa cgacattact tccatcacaa gcaacggcgg aagagttaac tgcatgcagg
     7021 tcatttatat ttcttctttc acttttttat tattccatat gatttttttc ggttctttct
     7081 tcgaatctac ataaactaat atcattggaa aaatcgaaaa aataggtgtg gcctccgatt
     7141 ggaaagaaga agtttgagac tctctcttac cttcctgacc ttaccgattc cgaaggtgct
     7201 caagaccaga gagaaaactc cattgatgtt gttcagcaag gacaacaaaa agggaaccaa
     7261 ggatcaagtg tcgaaaaaag acctcaacaa cgcttaacca tggatgtctc tcctttcgga
     7321 ttgttggatc ctttgtcacc aatgaggacg atgcgacaaa tgttagatac tatggacagg
     7381 atgttcgagg acactatgcc tgtctcagga agaaacagag gaggaagtgg agtgtcagag
     7441 attcgtgcac cgtgggacat caaagaggaa gaacacgaga tcaagatgcg tttcgacatg
     7501 cctggactct ctaaagagga cgtcaaaatc tctgtagaag ataacgtact tgtgatcaaa
     7561 ggagagcaga agaaggagga cagtgatgat tcttggtctg gaagaagcgt tagctcatat
     7621 ggaacacgac ttcagctccc agacaactgt gagaaagaca agatcaaagc tgagctcaag
     7681 aacggagtcc tctttatcac tatccctaag accaaagtcg aacgcaaagt catcgatgtc
     7741 cagattcaat cttcgggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     7801 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     7861 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     7921 atcactctcg gcatggacga gctgtacaag tacccatacg atgttcctga ctatgcgggc
     7981 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
     8041 gcttaagctt ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg
     8101 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     8161 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     8221 tcctaaaacc aaaatccagt actaaaatcc agatcgcttt acgaattccc atggggagga
     8281 attccaatcc cacaaaaatc tgagcttaac agcacagttg ctcctctcag agcagaatcg
     8341 ggtattcaac accctcatat caactactac gttgtgtata acggtccaca tgccggtata
     8401 tacgatgact ggggttgtac aaaggcggca acaaacggcg ttcccggagt tgcacacaag
     8461 aaatttgcca ctattacaga ggcaagagca gcagctgacg cgtacacaac aagtcagcaa
     8521 acagacaggt tgaacttcat ccccaaagga gaagctcaac tcaagcccaa gagctttgct
     8581 aaggccctaa caagcccacc aaagcaaaaa gcccactggc tcacgctagg aaccaaaagg
     8641 cccagcagtg atccagcccc aaaagagatc tcctttgccc cggagattac aatggacgat
     8701 ttcctctatc tttacgatct aggaaggaag ttcgaaggtg aaggtgacga cactatgttc
     8761 accactgata atgagaaggt tagcctcttc aatttcagaa agaatgctga cccacagatg
     8821 gttagagagg cctacgcagc aagtctcatc aagacgatct acccgagtaa caatctccag
     8881 gagatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaattgc
     8941 atcaagaaca cagagaaaga catatttctc aagatcagaa gtactattcc agtatggacg
     9001 attcaaggct tgcttcataa accaaggcaa gtaatagaga ttggagtctc taaaaaggta
     9061 gttcctactg aatctaaggc catgcatgga gtctaagatt caaatcgagg atctaacaga
     9121 actcgccgtc aagactggcg aacagttcat acagagtctt ttacgactca atgacaagaa
     9181 gaaaatcttc gtcaacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga
     9241 tacagtctca gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa
     9301 cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga
     9361 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc
     9421 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     9481 ggttccaacc acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga
     9541 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     9601 tttggagagg acacgcccat gggaaaaact tcgggagcga aacaggcgac tgtggtggtc
     9661 gcagcgatgg cgttaggatg gttagccata gagatcgctt tcaagccttt cctcgataaa
     9721 ttccgctcct caatcgacaa atctgaccca accaaagacc ccgatgactt cgacaccgcc
     9781 gctactgcaa ccacatccaa agagggttta atggtgagca agggcgagga gctgttcacc
     9841 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     9901 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     9961 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag
    10021 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
    10081 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
    10141 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
    10201 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactactt tagcgacaac
    10261 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
    10321 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
    10381 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
    10441 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
    10501 actctcggca tggacgagct gtacaaatga gcttgtcaag cagatcgttc aaacatttgg
    10561 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
    10621 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
    10681 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
    10741 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgacgc
    10801 tcagagagga tgcacatgtg accgagggac acgaagtgat ccgtttaaac tatcagtgtt
    10861 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
    10921 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca gccgcctttg
    10981 cgacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa
    11041 acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgtggat
    11101 acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg
    11161 ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt
    11221 ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga
    11281 tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta
    11341 ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg
    11401 cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt
    11461 tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta
    11521 taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg
    11581 gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg
    11641 ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc caattcccga
    11701 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    11761 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat ggctaaaatg
    11821 agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa
    11881 ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa
    11941 atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg
    12001 ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc
    12061 tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat
    12121 gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc
    12181 atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat
    12241 tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga ggacactcca
    12301 tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt
    12361 gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt
    12421 ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc
    12481 gtccggtcgc tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta
    12541 ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt
    12601 tagctgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    12661 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    12721 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    12781 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    12841 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    12901 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    12961 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    13021 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    13081 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    13141 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    13201 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    13261 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    13321 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    13381 tttttacggt tcctggcaga tcctagatgt ggcgcaacga tgccggcgac aagcaggagc
    13441 gcaccgactt cttccgcatc aagtgttttg gctctcaggc cgaggcccac ggcaagtatt
    13501 tgggcaaggg gtcgctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg
    13561 acggccagac ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca
    13621 aggcaccagg cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa
    13681 tcccgcaagg agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga
    13741 tcgacgcggg gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg
    13801 cgccccgcga aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg
    13861 agcgcgacag cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc
    13921 gttcgcgtcg tcttgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc
    13981 gaggaactat gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca
    14041 gcgaggccaa gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc
    14101 tttccttgtt cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg
    14161 cccgctctgc cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca
    14221 aggtcatttt ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg
    14281 ccgacgatga cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg
    14341 gcgagccgat caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg
    14401 gccggtatta cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct
    14461 tcacgtccga ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc
    14521 tggaccgtgg caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc
    14581 tgtttgctgg cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga
    14641 cggcccgacg gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg
    14701 aaaccttccg cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg
    14761 tcggcgaagc ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg
    14821 atgacctggt gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag
    14881 cagcccctgc tcggatctgt tggaccggac agtagtcatg gttgatgggc tgcctgtatc
    14941 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
    15001 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
    15061 aatgtactgg ggttgaacac tct
//
