LOCUS       sequence-357796-        5544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector of PIK3CA H1047R for gateway cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Wang C, Che L, Hu J, Zhang S, Jiang L, Latte G, Demartis MI, Tao J, 
            Gui B, Pilo MG, Ribback S, Dombrowski F, Evert M, Calvisi DF, Chen X
  TITLE     Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to 
            induce liver tumor formation in mice via AKT2/mTORC1 cascade.
  JOURNAL   Liver Int. 2015 Dec 30. doi: 10.1111/liv.13055.
  PUBMED    26716908
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Liver Int. 
            2015 Dec 30. doi: 10.1111/liv.13055."
COMMENT     Sequence Label: pT3EF5a-PIK3CA H1047R
FEATURES             Location/Qualifiers
     source          1..5544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(62..148)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(313..901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(393..412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(991..1800)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1714..1733
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1792..1811
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1923..2022
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5265..5291)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5338..5437)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5456..5479)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(5487..5514)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
       61 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      121 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcttagtag
      181 ttagacgtcc ccgagatcca tgctagcggt aatacggtta tccacagaat caggggataa
      241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      301 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      481 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      721 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      841 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      901 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgggg cccaatctga
      961 ataatgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     1021 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     1081 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     1141 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     1201 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     1261 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     1321 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     1381 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcagggaca ctgccagcgc
     1441 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc
     1501 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     1561 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     1621 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     1681 gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     1741 atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat
     1801 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     1861 tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct gcagctggat
     1921 ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc
     1981 aatgctttct tataatgcca actttgtaca agaaagctgg gtctagatat ctcgactcag
     2041 ttcaatgcat gctgtttaat tgtgtggaag atccaatcca tttttgttgt ccagccacca
     2101 tgacgtgcat cattcatttg tttcatgaaa tactccaaag cctcttgctc agttttatct
     2161 aaggctaggg tctttcgaat gtatgcaatg tcatcaaaag attgtagttc tggcattcca
     2221 gagccaagca tcattgagaa aagatttatg aagagattgg catgctgtcg aatagctaga
     2281 taagccttgt aacacatctc ctgaaacctc tcaaattctc ttgtctttgt gcattcttgg
     2341 gctcctttac taatcactat taagaaatcc tgtgtcaaaa caaatggcac acgttctcgt
     2401 ttataaccaa attttttctt cttgtgatcc aaaaagtgtc caaaatctat atgaaacagt
     2461 tgtccatcgt ctttcaccat gatgttacta ttgtgacgat ctccaattcc caaaatgaag
     2521 gtagctacac agtatccagc acatgaacgt gtaaacaggt caatggctgc atcatatatt
     2581 tctcctttgt tcttgtcttt gagccactga tgtagtgtgt ggctgttgaa ctgcagtgca
     2641 cctttcaagc cgcctttgca ctgaatttgc ataatagtgt gagaatttcg caccacctca
     2701 ataagtccca cacagtcacc gattgacaga caaccataag gtaacattcg aagatcaaga
     2761 ccttgatttt gccagatatt ttccataata cgaataattt gaagtgttag catatcttgc
     2821 cgtaaatcat ccccattttt aaagatgatc tcattgttct gaaacagtaa ctctgacatg
     2881 atgtctgggt tctcccaatt caaccacagt ggcctttttg cagaggacat aattcgacac
     2941 tcttcaagcc tgaggtttcc tagttgatga gcagggttta gaggagacag aaagccctgt
     3001 agagcatcca tgaaatctgg tcgcctcatt tgctcaacta aaaacttcat ctgtaccttt
     3061 tgtgtttcat ccttcttctc ctgtttgaga atgtcagtta agttaatgag cttttccatt
     3121 gcctcgactt gcctattcag gtgcttcaaa tacatcccac atgcacgaca ataggactcc
     3181 aaaagcaggc caaacctctg gctaactgtt ttattgtgca tctcagattt taaatgccaa
     3241 aagaaaaagt gcccaatcct ttgattagtc aatgctttct tcagtaaaaa tctcacaagc
     3301 aagttatcca aatattgttc atattttagg acctgtacta gctgaattaa atactgagaa
     3361 agtttgtcat ctgttaaata tttttccaag caccgaacag caaaacctcg aaccatagga
     3421 tctgggtaat tacagtccag aagttccata gcctgttcag gtttgattgg aggccaatct
     3481 tttaccaagc aatacatctg ggctacttca tctctagaat tccatttaac agacagaagc
     3541 aatttgggta gaatttcggg gatagttaca caatagtgtc tgtgactcca tagaaaatct
     3601 ttctcctgct cagtgatttc agagagagga tctcgtgtag aaattgcttt gagctgttct
     3661 ttgtcatttt cccttaattc attgtctcta gctagtctgt tactcagtcc tgcgtgggaa
     3721 tagctaaatc ctgcttctcg ggatacagac caattggcat gctcttcaat cactgacata
     3781 tctgggaact ttaccacact gctgaaccag tcaaactcca actctaagca tggagtttct
     3841 ttatttggat ttgatccagt aacaccaata gggttcagca aatcttctaa tccatgaggt
     3901 actggccaaa gattcaaagc catttttcca gatactagag tgtctgtgta atcaaacaag
     3961 tttatatttc cccatgccaa tggacagtgt tcctctttag caccctttcg gcctttaaca
     4021 gagcaaatgg aaaggcaaag tcgagcagca cgaggaagat caggaatgta tatatcataa
     4081 ttcagccatt cattccacct gggattggaa caaggtactc tttgagtgtt cacattgtca
     4141 cataagggtt ctcctccatg gtagatacct gttcgaacat agatcttatc aatgtctcga
     4201 atatttacat tcacgtaggt tgcacaaaga atttttattc tgagtgcact atttataacc
     4261 caaagggatt ttgtagatgt ttctccattc atatatggtg tagctgtgga aatgcgtctg
     4321 gaataagatg gcattgtaaa acagtccatt ggcagttgag aataaaggct ttctttagcc
     4381 atcaacatca aattgggcat cctcccaagc attatacagc ttcttatata cttatactga
     4441 ctcagaggat atttttctag gaagtattca tcacatccac acacttttaa aatatacttg
     4501 ccctgatatt ctaaaacaca gagttttagt tgttcagagg atagcaacat acttcgagtt
     4561 tttttcctga ttgcttcagc aattacttgt tctggtacac agtcatggtt gattttcaga
     4621 gtatacttct gcttgtcatt atttggagaa actattaccc agatcaccac tattatttgc
     4681 cctttatcta atttattata tatgtgcttt ggcaattctg gtgaagattc tacatttgga
     4741 ggatagacat acattgctct actatgaggt gaattgaggt ccctaagatc cacagcttct
     4801 ttacaaacgt tcagaacatt tcttcggaag tcctgtactt ctggatcttt aaccatatca
     4861 aattcacaca ctggcatgcc gatagcaaaa ccaatttctc gattgaggat cttttcttca
     4921 cggttgccta ctggttcaat tacttttaaa aagggttgaa aaagccgaag gtcacaaagt
     4981 cgtcttgttt catcaaaaaa ttcttccctt tctgcttctt gagtaacact tacgaaaatg
     5041 taagaagatt catcttgaag aagttgatgg agggggtatt ttcttgcttc tttaaatagt
     5101 tcatgcttta tggttattaa tgtagcctca cggaggcatt ctaaagtcac tatcattcca
     5161 tttggtagta aacattctac taggattctt gggggcatca agtggatgcc ccacagttca
     5221 cctgatgatg gtcttggagg gtacgtacca ccacactggg atctggcgta atctggcacg
     5281 tcgtatgggt accccatggt gaagcttgga tccagtcgac tgaattggtt cctttaaagc
     5341 ctgctttttt gtacaaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg
     5401 aacaggtcac tatcagtcaa aataaaatca ttatttgggg cccgagctta agtaactaac
     5461 taacaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     5521 tttgatgcct ggcagtttat ggcg
//
