LOCUS       sequence-357798-        6155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter gene with yeast recombination site within 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: p{Mhc-F4-678-DsRed::FRT,attB}
FEATURES             Location/Qualifiers
     source          1..6155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    465..489
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    506..539
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(560..584)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2278..2311
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2352..2421
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2640..2660)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2640..2658)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2679..2695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2679..2695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2692..2714)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2703..2719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2727..2733,2734..2751,2752..2757))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2772..2793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2910..2927)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3081..3669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3161..3180)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3840..4631,4632..4700))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4463..4482
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4701..4805)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4832..5287
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4919..4938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5129..5150
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5413..5435
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5427..5444
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5428..5444
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5454..5473
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5454..5472
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 attcgtatcg gatccgtgat ctatatttag gattaaaacc ctcgaccgcc ggagtataaa
       61 tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc
      121 taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat cgggcggccg
      181 cactagagcc ggtcgccacc atgaggtctt ccaagaatgt tatcaaggag ttcatgaggt
      241 ttaaggttcg catggaagga acggtcaatg ggcacgagtt tgaaatagaa ggcgaaggag
      301 aggggaggcc atacgaaggc cacaataccg taaagcttaa ggtgagtagt acatgtgcat
      361 acatcttaag ttcacttgat ctataggaac tgcgattgca acatcaaatt gtctgcggcg
      421 tgagaactgc gacccacaaa aatcccaaac cgcggccgtt tatcaccact ttgtacaaga
      481 aagctgggtc ggcgcgccca cccttgaagt tcctattctc tagaaagtat aggaacttcg
      541 gtgaaggggg cggccgcgga gcctgctttt ttgtacaaac ttgtgataaa cgaattcttg
      601 aagacgaaag ggcacaaaca aatagtgaca cgaaacagat tattctggta gctgttctcg
      661 ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc attcattttc
      721 gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
      781 acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta ctttttgttc
      841 ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca ttgcaggtaa
      901 ccaagggggg acctttgcca tttgcttggg atattttgtc accacaattt cagtatggaa
      961 gcaaggtata tgtcaagcac cctgccgaca taccagacta taaaaagctg tcatttcctg
     1021 aaggatttaa atgggaaagg gtcatgaact ttgaagacgg tggcgtcgtt actgtaaccc
     1081 aggattccag tttgcaggat ggctgtttca tctacaaggt caagttcatt ggcgtgaact
     1141 ttccttccga tggacctgtt atgcaaaaga agacaatggg ctgggaagcc agcactgagc
     1201 gtttgtatcc tcgtgatggc gtgttgaaag gagagattca taaggctctg aagctgaaag
     1261 acggtggtca ttacctagtt gaattcaaaa gtatttacat ggcaaagaag cctgtgcagc
     1321 taccagggta ctactatgtt gactccaaac tggatataac aagccacaac gaagactata
     1381 caatcgttga gcagtatgaa agaaccgagg gacgccacca tctgttcctt tagcggccat
     1441 cgaattcgag ctcgcccact aagcgtcgcg ccacttcaac gctcgatggg agcgtcattg
     1501 gtgggcgggg taaccgtcga aatcagtgtt tacgcttcca atcgcaacaa aaaattcact
     1561 gcaacactga aaagcatacg aaaacgatga agattgtacg agaaaccata aagtatttta
     1621 tccacaaaga cacgtatagc agaaaagcca agttaactcg gcgataagtt gtgtacacaa
     1681 gaataaaatc ggccagattc agtgttgtca gaaataagaa aaccccacta tgtttttctt
     1741 tgccttttct ttctcccagc gatcattcat ttcgtggtga aagaacgggg tcattgcacg
     1801 gagtttcgac tgcgggaaag cagagctgcc gttcacttcg tctataatta gcgctttcta
     1861 ttttccccga ttcgggccgc tgctgcgctt ttccgcctgc tgtttgtggc aagtgtagca
     1921 gcaggctgtg cacgcagtgt ggcatgcact tggctttcca ccgttggtat cgattctctg
     1981 ggacgatgag tcattccttt cggggccaca gcataatcgt tgccagctca ccgaaatggt
     2041 gacttcattt cttaactgcc gtcaagcatg cgattgtaca tacatacata tttatatatg
     2101 tacatattta tgtgactatg gtaggtcgat ataatagcaa tcaacgcaag caaatgtgtc
     2161 agtcctgctt acaggaacga ttctatttag taattttcgt tgtataaagt aattatgtat
     2221 gtatgtaagc cccataaatc tgaaacaatt aggcaaaacc atgcgaagct ctctagtata
     2281 acttcgtata atgtatgcta tacgaagttc tcgagggggg gcccggtacc gtcgacgatg
     2341 taggtcacgg tctcgaagcc gcggtgcggg tgccagggcg tgcccttggg ctccccgggc
     2401 gcgtactcca cctcacccat ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg
     2461 atcccggcga acgcgcggcg cgccgggaag ccctcgccct cgaaaccgct gggcgcggtg
     2521 gtcacggtga gcacgggacg tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc
     2581 agcgggttct cgacggtcac ggcgggcatg tcgacactag tggtacccag cttttgttcc
     2641 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     2701 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2761 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2821 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2881 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2941 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3001 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3061 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3121 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3181 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3241 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3301 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3361 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3421 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3481 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3541 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3601 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3661 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3721 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3781 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3841 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3901 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3961 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4021 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4081 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4141 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4201 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4261 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4321 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4381 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4441 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4501 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4561 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4621 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4681 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4741 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4801 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     4861 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4921 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4981 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5041 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5101 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5161 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5221 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5281 acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     5341 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     5401 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
     5461 actcactata gggcgaattg gagctccacc gcggtggcgg ccgctttaat atactcgaat
     5521 tcgttgtcgg ctcatataca tgggcgaaat aatttcgaat atgttttaaa aataaccaaa
     5581 gacattagaa agagatcgcc aatacttata cattatgtct atgtgtgcca tgtggtagca
     5641 tgagccaaaa agcttctcga aattacgaat tacatataga cgaatatgta tgtgacttta
     5701 gtttcgaaat aatttcgaga attttaaaaa taacgcattc gttaaaagtt cgcgtcaatt
     5761 cgaatcgaat tttcgattgt cgatttagtg tggattgtcg aaaatcgttc cgccttcgaa
     5821 gtttactgaa aggaatcatt gcgatctcgt gaattgcttg tatgagaaca cgccaccata
     5881 tcgagatacc tgttcaaaat atcgaaatcg cgttccaaac ggtagtagtt accagttgag
     5941 tgagttgtgg tgacagttta acctctctac atttgtaata ataagcattg ttttggcgta
     6001 tcaatagtct gtagattttt tcacaaagaa acctggagaa atgtacacaa atatctaaat
     6061 gaaaattggc gttacgtaac aaataccaac gccccgaaaa tccgtatata tgcaaagcat
     6121 tctgatatat atatataagt ggtatacata tacat
//
