LOCUS       sequence-357801-        9835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP driven by MEF2 transcription factor binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: pBac{3Xmef2-eGFP,attB,w+}
FEATURES             Location/Qualifiers
     source          1..9835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    726..795
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(1020..1038)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1490..1561,1963..2236,2311..2965,3027..3342,3546..3677,
                     3748..4362)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        5499..5737
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5684..5705
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          5752..5818
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             5849..6562
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          6649..6714
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6844..6864
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7136..7270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7173..7192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7227..7246
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7557..7591
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7627..7645)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7712..7816
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7817..7885,7886..8677)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8035..8054)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8848..9436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9337..9356
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   9503..9565
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 agaaacaaat tatacttatt agtcagtcag aaacaacttt ggcacatatc aatattatgc
       61 tctcgacaaa taactttttt gcattttttg cacgatgcat ttgcctttcg ccttatttta
      121 gaggggcagt aagtacagta agtacgtttt ttcattactg gctcttcagt actgtcatcc
      181 gatgtaccag gcacttcatt tggcaaaata ttagagatat tatcgcgcaa atatctcttc
      241 aaagtaggag cttctaaacg cttacgcata aacgatgacg tcaggctcat gtaaaggttt
      301 ctcataaatt ttttgcgact ttgaaccttt tctcccttgc tactgacatt atggctgtat
      361 ataataaaag aatttatgca ggcaatgttt atcattccgt acaataatgc cataggccac
      421 ctattcgtct tcctactgca ggtcatcaca gaacacattt ggtctagcgt gtccactccg
      481 cctttagttt gattataata cataaccatt tgcggtttac cggtactttc gttgatagaa
      541 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      601 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      661 tttttcagta cttccggtat ctcgcgtttg tttgatcgca cggttcccac gatgtaggtc
      721 acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac
      781 tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta gttgatcccg
      841 gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc ggtggtcacg
      901 gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag cgtcagcggg
      961 ttctcgacgg tcacggcggg catgtcgaca agccgaacat atgggcgcgc ctagtatgta
     1021 tgtaagttaa taaaacccat ttttgcggaa agtagataaa aaaaacattt ttttttttta
     1081 ctgcactgga tatcattgaa cttatctgat cagttttaaa tttacttcga tccaagggta
     1141 tttgatgtac caggttcttt cgattacctc tcactcaaaa tgacattcca ctcaaagtca
     1201 gcgctgtttg cctccttctc tgtccacaga aatatcgccg tctctttcgc cgctgcgtcc
     1261 gctatctctt tcgccaccgt ttgtagcgtt acgtagcgtc aatgtccgcc ttcagttgca
     1321 ttttgtcagc ggtttcgtga cgaagctcca agcggtttac gccatcaatt aaacacaaag
     1381 tgctgtgcca aaactcctct cgcttcttat ttttgtttgt tttttgagtg attggggtgg
     1441 tgattggttt tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga
     1501 ggatcaggag ctattaattc gcggaggcag caaacaccca tctgccgagc atctgaacaa
     1561 tgtgagtagt acatgtgcat acatcttaag ttcacttgat ctataggaac tgcgattgca
     1621 acatcaaatt gtctgcggcg tgagaactgc gacccacaaa aatcccaaac cgcaattgca
     1681 caaacaaata gtgacacgaa acagattatt ctggtagctg ttctcgctat ataagacaat
     1741 ttttgagatc atatcatgat caagacatct aaaggcattc attttcgact atattctttt
     1801 ttacaaaaaa tataacaacc agatatttta agctgatcct agatgcacaa aaaataaata
     1861 aaagtataaa cctacttcgt aggatacttc ggggtacttt ttgttcgggg ttagatgagc
     1921 ataacgcttg tagttgatat ttgagatccc ctatcattgc agggtgacag cggagcggct
     1981 tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac gctccggcca
     2041 cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa cctcacctat
     2101 gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg atggcggcag
     2161 ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc gcccaggaaa
     2221 catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat
     2281 aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt
     2341 tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc cttgcctttc
     2401 gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat ggccaacctg
     2461 tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac ctctttatcg
     2521 gctccctaac ggccagggaa cacctgattt tccaagccat ggtgcggatg ccacgacatc
     2581 tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca
     2641 aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa
     2701 ggaagcgtct ggcattcgcc tccgaggctc taaccgatcc gccgcttctg atctgcgatg
     2761 agcccacctc cggactggac tcctttaccg cccacagcgt cgtccaggtg ctgaagaagc
     2821 tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc gagctgtttg
     2881 agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg ggcactccca
     2941 gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg tatctagtat
     3001 tacataacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg
     3061 gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga gtcccgtgat
     3121 cggatcgcca agatatgcga caattttgcc attagcaaag tagcccggga tatggagcag
     3181 ttgttggcca ccaaaaatct ggagaagcca ctggagcagc cggagaatgg gtacacctac
     3241 aaggccacct ggttcatgca gttccgggcg gtcctgtggc gatcctggct gtcggtgctc
     3301 aaggaaccac tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg ttccagtgga
     3361 aacaaatgat ataacgctta caattcttgg aaacaaattc gctagatttt agatagaatt
     3421 gcctgattcc acacccttct tagttttttt caatgagatg tatagtttat agttttgcag
     3481 aagataaata aatttcattt aactcgcgaa tattaatgag atgcgagtaa cattttaatt
     3541 tgcagatggt tgccatcttg attggcctca tctttttggg ccaacaactc acgcaagtgg
     3601 gtgtgatgaa tatcaacgga gccatcttcc tcttcctgac caacatgacc tttcaaaacg
     3661 tctttgccac gataaatgta agtcatgttt agaatacatt tgcatttcaa taatttacta
     3721 actttctaat gaatcgattc gatttaggtg ttcacctcag agctgccagt ttttatgagg
     3781 gaggcccgaa gtcgacttta tcgctgtgac acatactttc tgggcaaaac gattgccgaa
     3841 ttgccgcttt ttctcacagt gccactggtc ttcacggcga ttgcctatcc gatgatcgga
     3901 ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc tggtcactct ggtggccaat
     3961 gtgtcaacgt ccttcggata tctaatatcc tgcgccagct cctcgacctc gatggcgctg
     4021 tctgtgggtc cgccggttat cataccattc ctgctctttg gcggcttctt cttgaactcg
     4081 ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg ttacgccaac
     4141 gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag ctgcacatcg
     4201 tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa cttctccgcc
     4261 gccgatctgc cgctggacta cgtgggtctg gccattctca tcgtgagctt ccgggtgctc
     4321 gcatatctgg ctctaagact tcgggcccga cgcaaggagt agccgacata tatccgaaat
     4381 aactgcttgt tttttttttt taccattatt accatcgtgt ttactgttta ttgccccctc
     4441 aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta tagaatacaa
     4501 gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta accaaaagac
     4561 ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa caccgggata
     4621 tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac ctccaccaca
     4681 ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca cattcacctt
     4741 ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc gctcaaatgg
     4801 ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta tgtacaattt
     4861 atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct aatttaatat
     4921 tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt acaatttgaa
     4981 ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa atggtgggca
     5041 taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat acatttaatt
     5101 tagaaaatgc ttggatttca ctggaactag ggcgcgcctc cggaacataa tggtgcaggg
     5161 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     5221 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     5281 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     5341 cacgatcatg cgcacccgtg gccagggccg cacatatgca atggttaatt cgagctcgcc
     5401 cggggcacgg ctaaaaatag cgttgtatag gctaaaaata gcctgacgta agctaaaaat
     5461 agcgttatcc aagcttatcg atttcgaacc ctcgaccgcc ggagtataaa tagaggcgct
     5521 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
     5581 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta
     5641 aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg
     5701 ccaagaagta attattgaat acaagaagag aactctgaat agatctaaaa ggtaggttca
     5761 accactgatg cctaggcaca ccgaaacgac taaccctaat tcttatcctt tacttcaggc
     5821 ggccgcggct cgagggtacc aatcaaacat gtccaaaggt gaagaactgt ttaccggagt
     5881 agtcccgata ttggttgaac tcgacggcga tgtcaacggt cataaattca gtgtgtccgg
     5941 cgagggtgag ggcgacgcca catacggtaa gctgacgttg aagttcatat gcaccacggg
     6001 caagctgccc gtgccatggc cgacgttggt cacgacgctg acgtatggtg tccagtgttt
     6061 cagccgttac cccgatcata tgaagcagca tgactttttc aagtcggcga tgccggaggg
     6121 atacgttcaa gagaggacca ttttcttcaa ggatgacggc aactataaga cgcgagcgga
     6181 ggtgaaattt gaaggcgaca cactcgttaa ccgtattgag ttgaagggca ttgattttaa
     6241 ggaggatggc aacattctgg gccataagtt ggaatacaac tacaactcgc ataatgtgta
     6301 tataatggca gataagcaga aaaacggaat aaaggttaac ttcaagattc gccacaacat
     6361 agaggacggt tccgtgcaac ttgcagatca ttaccaacag aacacaccca ttggagatgg
     6421 cccagttctc ttgccagaca atcactacct ttccacacag tccgcgttga gcaaggaccc
     6481 caatgaaaag cgggaccaca tggtgttgct ggagtttgtg accgcagctg gtattacaca
     6541 cggcatggat gagctctaca agtaatctag aggatctttg tgaaggaacc ttacttctgt
     6601 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     6661 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     6721 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     6781 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     6841 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     6901 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     6961 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatatttgat gtatagtgcc
     7021 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     7081 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     7141 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7201 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7261 tgtctggatc gatctggccg gcgatatcta taacaagaaa atatatatat aataagttat
     7321 cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca
     7381 cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga
     7441 cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta
     7501 aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg
     7561 cgtcaatttt acgcagacta tctttctagg ggccggccgt ttaaacgaat tcttgaagac
     7621 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7681 agactcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7741 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7801 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7861 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7921 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7981 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8041 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8101 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8161 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8221 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8281 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8341 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8401 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8461 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8521 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8581 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8641 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8701 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8761 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8821 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8881 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8941 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     9001 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9061 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     9121 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9181 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9241 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9301 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9361 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9421 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9481 gccttttgct cacatgttac cgccctagaa agataatcat attgtgacgt acgttaaaga
     9541 taatcatgcg taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt
     9601 aatttgaata gatattaagt tttattatat ttacacttac atactaataa taaattcaac
     9661 aaacaattta tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct
     9721 ataaagtaac aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt
     9781 taaaaacagt catgttgtat tataaaataa gtaattagct taacttatac ataat
//
