LOCUS       sequence-357803-        9776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP driven by tinman and GAGA transcription factor binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: pBac{3Xtin-GAGA-eGFP,w+,attB}
FEATURES             Location/Qualifiers
     source          1..9776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        530..768
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     715..736
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          783..849
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             880..1593
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          1680..1745
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1875..1895
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2167..2301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2204..2223)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2258..2277
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   2588..2622
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2658..2676)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2743..2847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2848..2916,2917..3708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3066..3085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3879..4467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4368..4387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4534..4596
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    5592..5661
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(5886..5904)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6356..6427,6829..7102,7177..7831,7893..8208,8412..8543,
                     8614..9228)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 aatttaatat tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt
       61 acaatttgaa ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa
      121 atggtgggca taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat
      181 acatttaatt tagaaaatgc ttggatttca ctggaactag ggcgcgcctc cggaacataa
      241 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
      301 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
      361 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
      421 acgacaggag cacgatcatg cagagagtct ctcaagtgtg tagagagtca ctcaagtgtg
      481 actctctact ctcaagtgat tagagatatc gatttcgaac cctcgaccgc cggagtataa
      541 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
      601 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa
      661 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
      721 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa
      781 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct
      841 ttacttcagg cggccgcggc tcgagggtac caatcaaaca tgtccaaagg tgaagaactg
      901 tttaccggag tagtcccgat attggttgaa ctcgacggcg atgtcaacgg tcataaattc
      961 agtgtgtccg gcgagggtga gggcgacgcc acatacggta agctgacgtt gaagttcata
     1021 tgcaccacgg gcaagctgcc cgtgccatgg ccgacgttgg tcacgacgct gacgtatggt
     1081 gtccagtgtt tcagccgtta ccccgatcat atgaagcagc atgacttttt caagtcggcg
     1141 atgccggagg gatacgttca agagaggacc attttcttca aggatgacgg caactataag
     1201 acgcgagcgg aggtgaaatt tgaaggcgac acactcgtta accgtattga gttgaagggc
     1261 attgatttta aggaggatgg caacattctg ggccataagt tggaatacaa ctacaactcg
     1321 cataatgtgt atataatggc agataagcag aaaaacggaa taaaggttaa cttcaagatt
     1381 cgccacaaca tagaggacgg ttccgtgcaa cttgcagatc attaccaaca gaacacaccc
     1441 attggagatg gcccagttct cttgccagac aatcactacc tttccacaca gtccgcgttg
     1501 agcaaggacc ccaatgaaaa gcgggaccac atggtgttgc tggagtttgt gaccgcagct
     1561 ggtattacac acggcatgga tgagctctac aagtaatcta gaggatcttt gtgaaggaac
     1621 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     1681 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     1741 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     1801 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     1861 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     1921 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     1981 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga
     2041 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     2101 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     2161 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2221 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     2281 gtatcttatc atgtctggat cgatctggcc ggcgatatct ataacaagaa aatatatata
     2341 taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat
     2401 cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc
     2461 aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact
     2521 gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc
     2581 aagaatgcat gcgtcaattt tacgcagact atctttctag gggccggccg tttaaacgaa
     2641 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2701 aatggtttct tagactcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2761 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2821 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2881 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2941 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3001 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3061 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3121 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3181 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3241 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3301 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3361 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3421 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3481 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3541 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3601 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3661 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3721 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3781 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4021 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4501 gccttttgct ggccttttgc tcacatgtta ccgccctaga aagataatca tattgtgacg
     4561 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
     4621 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     4681 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     4741 aaatttcttc tataaagtaa caaaactttt aaacattctc tcttttacaa aaataaactt
     4801 attttgtact ttaaaaacag tcatgttgta ttataaaata agtaattagc ttaacttata
     4861 cataatagaa acaaattata cttattagtc agtcagaaac aactttggca catatcaata
     4921 ttatgctctc gacaaataac ttttttgcat tttttgcacg atgcatttgc ctttcgcctt
     4981 attttagagg ggcagtaagt acagtaagta cgttttttca ttactggctc ttcagtactg
     5041 tcatccgatg taccaggcac ttcatttggc aaaatattag agatattatc gcgcaaatat
     5101 ctcttcaaag taggagcttc taaacgctta cgcataaacg atgacgtcag gctcatgtaa
     5161 aggtttctca taaatttttt gcgactttga accttttctc ccttgctact gacattatgg
     5221 ctgtatataa taaaagaatt tatgcaggca atgtttatca ttccgtacaa taatgccata
     5281 ggccacctat tcgtcttcct actgcaggtc atcacagaac acatttggtc tagcgtgtcc
     5341 actccgcctt tagtttgatt ataatacata accatttgcg gtttaccggt actttcgttg
     5401 atagaagcat cctcatcaca agatgataat aagtatacca tcttagctgg cttcggttta
     5461 tatgagacga gagtaagggg tccgtcaaaa caaaacatcg atgttcccac tggcctggag
     5521 cgactgtttt tcagtacttc cggtatctcg cgtttgtttg atcgcacggt tcccacgatg
     5581 taggtcacgg tctcgaagcc gcggtgcggg tgccagggcg tgcccttggg ctccccgggc
     5641 gcgtactcca cctcacccat ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg
     5701 atcccggcga acgcgcggcg caccgggaag ccctcgccct cgaaaccgct gggcgcggtg
     5761 gtcacggtga gcacgggacg tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc
     5821 agcgggttct cgacggtcac ggcgggcatg tcgacaagcc gaacatatgg gcgcgcctag
     5881 tatgtatgta agttaataaa acccattttt gcggaaagta gataaaaaaa acattttttt
     5941 tttttactgc actggatatc attgaactta tctgatcagt tttaaattta cttcgatcca
     6001 agggtatttg atgtaccagg ttctttcgat tacctctcac tcaaaatgac attccactca
     6061 aagtcagcgc tgtttgcctc cttctctgtc cacagaaata tcgccgtctc tttcgccgct
     6121 gcgtccgcta tctctttcgc caccgtttgt agcgttacgt agcgtcaatg tccgccttca
     6181 gttgcatttt gtcagcggtt tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac
     6241 acaaagtgct gtgccaaaac tcctctcgct tcttattttt gtttgttttt tgagtgattg
     6301 gggtggtgat tggttttggg tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg
     6361 ccaagaggat caggagctat taattcgcgg aggcagcaaa cacccatctg ccgagcatct
     6421 gaacaatgtg agtagtacat gtgcatacat cttaagttca cttgatctat aggaactgcg
     6481 attgcaacat caaattgtct gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca
     6541 attgcacaaa caaatagtga cacgaaacag attattctgg tagctgttct cgctatataa
     6601 gacaattttt gagatcatat catgatcaag acatctaaag gcattcattt tcgactatat
     6661 tcttttttac aaaaaatata acaaccagat attttaagct gatcctagat gcacaaaaaa
     6721 taaataaaag tataaaccta cttcgtagga tacttcgggg tactttttgt tcggggttag
     6781 atgagcataa cgcttgtagt tgatatttga gatcccctat cattgcaggg tgacagcgga
     6841 gcggcttcgc agagctgcat taaccagggc ttcgggcagg ccaaaaacta cggcacgctc
     6901 cggccaccca gtccgccgga ggactccggt tcagggagcg gccaactagc cgagaacctc
     6961 acctatgcct ggcacaatat ggacatcttt ggggcggtca atcagccggg ctccggatgg
     7021 cggcagctgg tcaaccggac acgcggacta ttctgcaacg agcgacacat accggcgccc
     7081 aggaaacatt tgctcaagaa cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat
     7141 cttaataaag ggtccaatta ccaatttgaa actcagtttg cggcgtggcc tatccgggcg
     7201 aacttttggc cgtgatgggc agttccggtg ccggaaagac gaccctgctg aatgcccttg
     7261 cctttcgatc gccgcagggc atccaagtat cgccatccgg gatgcgactg ctcaatggcc
     7321 aacctgtgga cgccaaggag atgcaggcca ggtgcgccta tgtccagcag gatgacctct
     7381 ttatcggctc cctaacggcc agggaacacc tgattttcca agccatggtg cggatgccac
     7441 gacatctgac ctatcggcag cgagtggccc gcgtggatca ggtgatccag gagctttcgc
     7501 tcagcaaatg tcagcacacg atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg
     7561 gagaaaggaa gcgtctggca ttcgcctccg aggctctaac cgatccgccg cttctgatct
     7621 gcgatgagcc cacctccgga ctggactcct ttaccgccca cagcgtcgtc caggtgctga
     7681 agaagctgtc gcagaagggc aagaccgtca tcctgaccat tcatcagccg tcttccgagc
     7741 tgtttgagct ctttgacaag atccttctga tggccgaggg cagggtagct ttcttgggca
     7801 ctcccagcga agccgtcgac ttcttttcct agtgagttcg atgtgtttat taagggtatc
     7861 tagtattaca taacatctca actcctatcc agcgtgggtg cccagtgtcc taccaactac
     7921 aatccggcgg acttttacgt acaggtgttg gccgttgtgc ccggacggga gatcgagtcc
     7981 cgtgatcgga tcgccaagat atgcgacaat tttgccatta gcaaagtagc ccgggatatg
     8041 gagcagttgt tggccaccaa aaatctggag aagccactgg agcagccgga gaatgggtac
     8101 acctacaagg ccacctggtt catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg
     8161 gtgctcaagg aaccactcct cgtaaaagtg cgacttattc agacaacggt gagtggttcc
     8221 agtggaaaca aatgatataa cgcttacaat tcttggaaac aaattcgcta gattttagat
     8281 agaattgcct gattccacac ccttcttagt ttttttcaat gagatgtata gtttatagtt
     8341 ttgcagaaga taaataaatt tcatttaact cgcgaatatt aatgagatgc gagtaacatt
     8401 ttaatttgca gatggttgcc atcttgattg gcctcatctt tttgggccaa caactcacgc
     8461 aagtgggtgt gatgaatatc aacggagcca tcttcctctt cctgaccaac atgacctttc
     8521 aaaacgtctt tgccacgata aatgtaagtc atgtttagaa tacatttgca tttcaataat
     8581 ttactaactt tctaatgaat cgattcgatt taggtgttca cctcagagct gccagttttt
     8641 atgagggagg cccgaagtcg actttatcgc tgtgacacat actttctggg caaaacgatt
     8701 gccgaattgc cgctttttct cacagtgcca ctggtcttca cggcgattgc ctatccgatg
     8761 atcggactgc gggccggagt gctgcacttc ttcaactgcc tggcgctggt cactctggtg
     8821 gccaatgtgt caacgtcctt cggatatcta atatcctgcg ccagctcctc gacctcgatg
     8881 gcgctgtctg tgggtccgcc ggttatcata ccattcctgc tctttggcgg cttcttcttg
     8941 aactcgggct cggtgccagt atacctcaaa tggttgtcgt acctctcatg gttccgttac
     9001 gccaacgagg gtctgctgat taaccaatgg gcggacgtgg agccgggcga aattagctgc
     9061 acatcgtcga acaccacgtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc
     9121 tccgccgccg atctgccgct ggactacgtg ggtctggcca ttctcatcgt gagcttccgg
     9181 gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc
     9241 cgaaataact gcttgttttt tttttttacc attattacca tcgtgtttac tgtttattgc
     9301 cccctcaaaa agctaatgta attatatttg tgccaataaa aacaagatat gacctataga
     9361 atacaagtat ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca
     9421 aaagacttac acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc
     9481 gggatatatt ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc
     9541 accacaccac gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt
     9601 caccttttgt tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggttcgctc
     9661 aaatggttcc gagtggttca tttcgtctca atagaaatta gtaataaata tttgtatgta
     9721 caatttattt gctccaatat atttgtatat atttccctca cagctatatt tattct
//
