LOCUS       sequence-357804-       15239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 module containing mCHERRY-nYFP HSP21-cYFP OEP7-mTRQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15239)
  AUTHORS   Velay F, Soula M, Mehrez M, Belbachir C, D'Alessandro S, Laloi C, 
            Crete P, Field B
  TITLE     MoBiFC: development of a modular bimolecular fluorescence 
            complementation toolkit for the analysis of chloroplast 
            protein-protein interactions.
  JOURNAL   Plant Methods. 2022 May 26;18(1):69. doi: 
            10.1186/s13007-022-00902-1.
  PUBMED    35619173
REFERENCE   2  (bases 1 to 15239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2022-05-26"; volume: "18(1):69. doi"; pages: " 
            10.1186/s13007-022-00902-1"
COMMENT     Sequence Label: pLBM0070
FEATURES             Location/Qualifiers
     source          1..15239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1011..1356
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      1978..2230
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3246..3591
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4031..4738
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4176..4194)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             4802..4825
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5382..5556
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        6569..6914
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             8107..8196
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    8237..8411
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        9424..9769
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(9967..9969,9970..9972,9973..10686)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10012..10033)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      10699..10951
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11017..11041
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      11129..11839
                     /label=oriV
                     /note="incP origin of replication"
     promoter        11860..11964
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11965..12759
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12930..13518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13419..13438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13854..15002
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    15148..15172
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga ggaattccaa tcccacaaaa atctgagctt aacagcacag
       61 ttgctcctct cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt
      121 ataacggtcc acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg
      181 gcgttcccgg agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg
      241 acgcgtacac aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc
      301 aactcaagcc caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact
      361 ggctcacgct aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg
      421 ccccggagat tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag
      481 gtgaaggtga cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca
      541 gaaagaatgc tgacccacag atggttagag aggcctacgc agcaagtctc atcaagacga
      601 tctacccgag taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag
      661 tcaaaagatt caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca
      721 gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag
      781 agattggagt ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag
      841 attcaaatcg aggatctaac agaactcgcc gtcaagactg gcgaacagtt catacagagt
      901 cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg
      961 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
     1021 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     1081 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     1141 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
     1201 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
     1261 gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     1321 tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt
     1381 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc
     1441 gatacaatgg aacgagctat acaaggaaac gatgctaggg aacaagctta tggtgaacgt
     1501 tggaatggag gatcaggaag ttccacttct cccttcaaac ttcctgacga aagtccgagt
     1561 tggactgagt ggcggctaca taacgatgag actatttcga atcaagataa tccccttggt
     1621 ttcaaggaaa gctggggttt cgggaaagtt gtatttaaga gatatctcag atacgacggg
     1681 acggaaactt cactgcacag agtccttgga tcttggacgg gagattcggt taactatgca
     1741 gcatctcgat ttctcggttt cgaccagatc ggatgtacct atagtattcg gtttcgagga
     1801 gttagtgtca ccatttctgg agggtcgcga actcttcagc atctcagtga aatggcaatt
     1861 cggtctaagc aagaactgct acagcttacc ccagtcaaag tggaaagtga tgtatcaaga
     1921 ggatgccctg aaggtgttga aaccttcgaa gaagaaagcg agtaagcttg tcaagcagat
     1981 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     2041 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     2101 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     2161 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     2221 ttactagatc gacgctgcaa gaattcaagc ttggaggaat tccaatccca caaaaatctg
     2281 agcttaacag cacagttgct cctctcagag cagaatcggg tattcaacac cctcatatca
     2341 actactacgt tgtgtataac ggtccacatg ccggtatata cgatgactgg ggttgtacaa
     2401 aggcggcaac aaacggcgtt cccggagttg cacacaagaa atttgccact attacagagg
     2461 caagagcagc agctgacgcg tacacaacaa gtcagcaaac agacaggttg aacttcatcc
     2521 ccaaaggaga agctcaactc aagcccaaga gctttgctaa ggccctaaca agcccaccaa
     2581 agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc cagcagtgat ccagccccaa
     2641 aagagatctc ctttgccccg gagattacaa tggacgattt cctctatctt tacgatctag
     2701 gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac cactgataat gagaaggtta
     2761 gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc tacgcagcaa
     2821 gtctcatcaa gacgatctac ccgagtaaca atctccagga gatcaaatac cttcccaaga
     2881 aggttaaaga tgcagtcaaa agattcagga ctaattgcat caagaacaca gagaaagaca
     2941 tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg cttcataaac
     3001 caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcctactgaa tctaaggcca
     3061 tgcatggagt ctaagattca aatcgaggat ctaacagaac tcgccgtcaa gactggcgaa
     3121 cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt caacatggtg
     3181 gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga agatcaaagg
     3241 gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt ccattgccca
     3301 gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
     3361 cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg tcccaaagat
     3421 ggacccccac ccacgaggag catcgtggaa aaagaagagg ttccaaccac gtctacaaag
     3481 caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct
     3541 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctcgagt
     3601 ataagagctc atttttacaa caattaccaa caacaacaaa caacaaacaa cattacaatt
     3661 acatttacaa ttatcgatac aatggcttcc tctatgctct cttccgctac tatggttgcc
     3721 tctccggctc aggccactat ggtcgctcct ttcaacggac ttaagtcctc cgctgccttc
     3781 ccagccaccc gcaaggctaa caacgacatt acttccatca caagcaacgg cggaagagtt
     3841 aactgcatgc aggtcattta tatttcttct ttcacttttt tattattcca tatgattttt
     3901 ttcggttctt tcttcgaatc tacataaact aatatcattg gaaaaatcga aaaaataggt
     3961 gtggcctccg attggaaaga agaagtttga gactctctct taccttcctg accttaccga
     4021 ttccgaaggt atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     4081 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     4141 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     4201 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     4261 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     4321 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     4381 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     4441 cggccccgta atgcagaaga aaaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     4501 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     4561 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     4621 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     4681 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg
     4741 ttcggattac aaggaccacg acggggacta caaggatcat gatttggatg ctgctgccgc
     4801 cgactataag gatgatgacg ataaggtgag caagggcgaa gagttgttca ctggtgtggt
     4861 tcctatcctc gttgagcttg atggtgatgt gaacgggcac aagttctccg tttctggtga
     4921 aggtgagggt gatgctactt acggaaagct caccctcaag ttcatctgta ccactggaaa
     4981 gctccctgtg ccttggccta ctctcgttac tactttcgga tacggactcc agtgcttcgc
     5041 tagataccct gatcatatga agcagcacga cttcttcaag agcgctatgc ctgagggata
     5101 cgtgcaagag agaaccattt tcttcaagga cgacggcaac tacaagacca gagctgaggt
     5161 taagttcgaa ggtgacaccc tcgtgaacag gatcgagctt aagggcatcg acttcaaaga
     5221 ggacggaaac atcctcggac acaagctcga gtacaactac aacagccaca acgtgtacat
     5281 catggccgac aagcagaaga acggcatcaa ggtcaacttc aagatcaggc acaacatcga
     5341 ggactaagct tctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt
     5401 gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat
     5461 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     5521 ttcctaaaac caaaatccag tactaaaatc cagatcgcta ctagaattcg agctcggagg
     5581 aattccaatc ccacaaaaat ctgagcttaa cagcacagtt gctcctctca gagcagaatc
     5641 gggtattcaa caccctcata tcaactacta cgttgtgtat aacggtccac atgccggtat
     5701 atacgatgac tggggttgta caaaggcggc aacaaacggc gttcccggag ttgcacacaa
     5761 gaaatttgcc actattacag aggcaagagc agcagctgac gcgtacacaa caagtcagca
     5821 aacagacagg ttgaacttca tccccaaagg agaagctcaa ctcaagccca agagctttgc
     5881 taaggcccta acaagcccac caaagcaaaa agcccactgg ctcacgctag gaaccaaaag
     5941 gcccagcagt gatccagccc caaaagagat ctcctttgcc ccggagatta caatggacga
     6001 tttcctctat ctttacgatc taggaaggaa gttcgaaggt gaaggtgacg acactatgtt
     6061 caccactgat aatgagaagg ttagcctctt caatttcaga aagaatgctg acccacagat
     6121 ggttagagag gcctacgcag caagtctcat caagacgatc tacccgagta acaatctcca
     6181 ggagatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca ggactaattg
     6241 catcaagaac acagagaaag acatatttct caagatcaga agtactattc cagtatggac
     6301 gattcaaggc ttgcttcata aaccaaggca agtaatagag attggagtct ctaaaaaggt
     6361 agttcctact gaatctaagg ccatgcatgg agtctaagat tcaaatcgag gatctaacag
     6421 aactcgccgt caagactggc gaacagttca tacagagtct tttacgactc aatgacaaga
     6481 agaaaatctt cgtcaacatg gtggagcacg acactctggt ctactccaaa aatgtcaaag
     6541 atacagtctc agaagatcaa agggctattg agacttttca acaaaggata atttcgggaa
     6601 acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca gtagaaaagg
     6661 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt caagatctct
     6721 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
     6781 aggttccaac cacgtctaca aagcaagtgg attgatgtga catctccact gacgtaaggg
     6841 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
     6901 atttggagag gacacgctcg agtataagag ctcattttta caacaattac caacaacaac
     6961 aaacaacaaa caacattaca attacattta caattatcga tacaatggct tcctctatgc
     7021 tctcttccgc tactatggtt gcctctccgg ctcaggccac tatggtcgct cctttcaacg
     7081 gacttaagtc ctccgctgcc ttcccagcca cccgcaaggc taacaacgac attacttcca
     7141 tcacaagcaa cggcggaaga gttaactgca tgcaggtcat ttatatttct tctttcactt
     7201 ttttattatt ccatatgatt tttttcggtt ctttcttcga atctacataa actaatatca
     7261 ttggaaaaat cgaaaaaata ggtgtggcct ccgattggaa agaagaagtt tgagactctc
     7321 tcttaccttc ctgaccttac cgattccgaa ggtgctcaag accagagaga aaactccatt
     7381 gatgttgttc agcaaggaca acaaaaaggg aaccaaggat caagtgtcga aaaaagacct
     7441 caacaacgct taaccatgga tgtctctcct ttcggattgt tggatccttt gtcaccaatg
     7501 aggacgatgc gacaaatgtt agatactatg gacaggatgt tcgaggacac tatgcctgtc
     7561 tcaggaagaa acagaggagg aagtggagtg tcagagattc gtgcaccgtg ggacatcaaa
     7621 gaggaagaac acgagatcaa gatgcgtttc gacatgcctg gactctctaa agaggacgtc
     7681 aaaatctctg tagaagataa cgtacttgtg atcaaaggag agcagaagaa ggaggacagt
     7741 gatgattctt ggtctggaag aagcgttagc tcatatggaa cacgacttca gctcccagac
     7801 aactgtgaga aagacaagat caaagctgag ctcaagaacg gagtcctctt tatcactatc
     7861 cctaagacca aagtcgaacg caaagtcatc gatgtccaga ttcaatcttc gggcagcgtg
     7921 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     7981 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     8041 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     8101 tacaagtacc catacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat
     8161 gcaggatcct atccatatga cgttccagat tacgctgctt aagcttctct agctagagtc
     8221 gatcgacaag ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta
     8281 gggttcctat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg
     8341 tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta
     8401 aaatccagat cgctttacga attcccatgg ggaggaattc caatcccaca aaaatctgag
     8461 cttaacagca cagttgctcc tctcagagca gaatcgggta ttcaacaccc tcatatcaac
     8521 tactacgttg tgtataacgg tccacatgcc ggtatatacg atgactgggg ttgtacaaag
     8581 gcggcaacaa acggcgttcc cggagttgca cacaagaaat ttgccactat tacagaggca
     8641 agagcagcag ctgacgcgta cacaacaagt cagcaaacag acaggttgaa cttcatcccc
     8701 aaaggagaag ctcaactcaa gcccaagagc tttgctaagg ccctaacaag cccaccaaag
     8761 caaaaagccc actggctcac gctaggaacc aaaaggccca gcagtgatcc agccccaaaa
     8821 gagatctcct ttgccccgga gattacaatg gacgatttcc tctatcttta cgatctagga
     8881 aggaagttcg aaggtgaagg tgacgacact atgttcacca ctgataatga gaaggttagc
     8941 ctcttcaatt tcagaaagaa tgctgaccca cagatggtta gagaggccta cgcagcaagt
     9001 ctcatcaaga cgatctaccc gagtaacaat ctccaggaga tcaaatacct tcccaagaag
     9061 gttaaagatg cagtcaaaag attcaggact aattgcatca agaacacaga gaaagacata
     9121 tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct tcataaacca
     9181 aggcaagtaa tagagattgg agtctctaaa aaggtagttc ctactgaatc taaggccatg
     9241 catggagtct aagattcaaa tcgaggatct aacagaactc gccgtcaaga ctggcgaaca
     9301 gttcatacag agtcttttac gactcaatga caagaagaaa atcttcgtca acatggtgga
     9361 gcacgacact ctggtctact ccaaaaatgt caaagataca gtctcagaag atcaaagggc
     9421 tattgagact tttcaacaaa ggataatttc gggaaacctc ctcggattcc attgcccagc
     9481 tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca
     9541 ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc gacagtggtc ccaaagatgg
     9601 acccccaccc acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca
     9661 agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc
     9721 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac gcccatggga
     9781 aaaacttcgg gagcgaaaca ggcgactgtg gtggtcgcag cgatggcgtt aggatggtta
     9841 gccatagaga tcgctttcaa gcctttcctc gataaattcc gctcctcaat cgacaaatct
     9901 gacccaacca aagaccccga tgacttcgac accgccgcta ctgcaaccac atccaaagag
     9961 ggtttaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
    10021 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
    10081 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
    10141 cccaccctcg tgaccaccct gtcctggggc gtgcagtgct tcgcccgcta ccccgaccac
    10201 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
    10261 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
    10321 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
    10381 gggcacaagc tggagtacaa ctactttagc gacaacgtct atatcaccgc cgacaagcag
    10441 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
    10501 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
    10561 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
    10621 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
    10681 aaatgagctt gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat
    10741 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
    10801 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
    10861 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
    10921 tcgcgcgcgg tgtcatctat gttactagat cgacgctcag agaggatgca catgtgaccg
    10981 agggacacga agtgatccgt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
    11041 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
    11101 atccgttcgt ccatttgtat gtgccagccg cctttgcgac gctcaccggg ctggttgccc
    11161 tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc
    11221 cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct cgcggaaaac ttggccctca
    11281 ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc gcggcgttga
    11341 cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg
    11401 gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc ctggggataa
    11461 gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg gcgcgatcct
    11521 tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca tttgaggggc
    11581 tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc ggccaccgct
    11641 aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt aaccagggct
    11701 gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct cgaaccctcc
    11761 cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc cgcgaacggc
    11821 ctcaccccaa aaatggcagc gctggccaat tcccgagtgc gcggaacccc tatttgttta
    11881 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    11941 caataatatt gaaaaaggaa gagtatggct aaaatgagaa tatcaccgga attgaaaaaa
    12001 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
    12061 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
    12121 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
    12181 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
    12241 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
    12301 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
    12361 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
    12421 gatgtggatt gcgaaaactg ggaagaggac actccattta aagatccgcg cgagctgtat
    12481 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
    12541 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
    12601 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgctcag ggaggatatc
    12661 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
    12721 aaaataaaat attatatttt actggatgaa ttgttttagc tgtcagacca agtttactca
    12781 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    12841 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    12901 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    12961 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    13021 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    13081 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    13141 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    13201 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    13261 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    13321 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    13381 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    13441 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    13501 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggcagatcct
    13561 agatgtggcg caacgatgcc ggcgacaagc aggagcgcac cgacttcttc cgcatcaagt
    13621 gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg ctggtattcg
    13681 tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc tacgggaccg
    13741 acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg tcaaatcagg
    13801 aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg tgaatgaatc
    13861 ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt tccgccgagg
    13921 atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc ttccagtccg
    13981 tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg caactggctc
    14041 cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctt gaacaggagg
    14101 cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg accaagaagc
    14161 gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag gccgcgttgc
    14221 tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat attgcgccgt
    14281 ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg ttcaccacgc
    14341 gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac gtcaacaagg
    14401 acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa ctggtgtggc
    14461 agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc ttcacgttct
    14521 acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg aaggccgagg
    14581 aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc gttgggcacc
    14641 tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag aaaacgtccc
    14701 gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac cactacacga
    14761 aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg ttcgactatt
    14821 tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc atgtgcggat
    14881 cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc gaagagttgc
    14941 gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat tgcaaacgct
    15001 agggccttgt ggggtcagtt ccggctgggg gttcagcagc ccctgctcgg atctgttgga
    15061 ccggacagta gtcatggttg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    15121 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
    15181 gcttagacaa cttaataaca cattgcggac gtttttaatg tactggggtt gaacactct
//
