LOCUS       sequence-357805-        9827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EcoRV digestion produces 12x601 chromatin assembly construct with 
            central TetO following PEG removal of plasmid backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: p12x601
FEATURES             Location/Qualifiers
     source          1..9827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1360..1754
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(join(1955..3442,3443..3490))
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     intron          3565..4040
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     complement(4153..4177)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4174..4377)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4203..4223)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4378..4681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(4822..4838)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4822..4838)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4835..4857)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4846..4862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4870..4876,4877..4894,4895..4900))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4915..4936
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5053..5070)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5224..5812)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5304..5323)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5950..6324)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      6321..6330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(6530..7324)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDALEFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLLEFF"
     primer_bind     complement(6641..6660)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7251..7270
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(7673..7975)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7775..7794)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     8098..8120
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8112..8129
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8113..8129
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8136..8155
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8136..8154
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9343..9361
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cgtgcgttta agcggtgcta gagctgtcta cgaccaattg agcggcctcg gcaccgggat
       61 tctccagtca aactttttgt tgaattcaag ctttttatca ccttcatcgc gctacaggat
      121 gtatatatct gacacgtgcc tggagactag ggagtaatcc ccttggcggt taaaacgcgg
      181 gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa ttgagcggcc
      241 tcggcaccgg gattctccag catacaaatg ctctgaattc atcgattttt cttcaagata
      301 ttccaaacag gatgtatata tctgacacgt gcctggagac tagggagtaa tccccttggc
      361 ggttaaaacg cgggggacag cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac
      421 caattgagcg gcctcggcac cgggattctc cagaagaagt cgagtgggaa ttcggatccc
      481 aaggccctta attttccaaa caggatgtat atatctgaca cgtgcctgga gactagggag
      541 taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag
      601 agctgtctac gaccaattga gcggcctcgg caccgggatt ctccagtaac ctagtatagg
      661 ggacatgata tctgcagaat tcaggcctca agaggaatta tacgaagcct gtgaaaaggt
      721 tgttttggaa cttagaaact acactgagca ttctacacca tttctgaata aagtgagcaa
      781 aagagaagcc cccaattatc atcaaatcat caaaaagtcc atggacctga atactgtttt
      841 aaaaaaactg aaaagctttc aatatgactc caaacaagaa tttgtagacg atattatgct
      901 aatatggaaa aattgtttga cctataattc agatccttca cattttttga gagggcatgc
      961 tattgctatg cagaagaaat ctcttcagtt gattcgcatg attccaaata ggcctgaatt
     1021 ccagcacact ggcggccgtt actagtgcta gactgccatg tcgagggatt ccgggtcact
     1081 gtgagtgggg gaggcaggga agaagggctc acaggacagt caaaccatgc cccctgtttt
     1141 tccttcttca agtagacctc tataagacaa cagagacaac taaggctgag tggccaggcg
     1201 aggagaaacc atctcgccgt aaaacatgga aggaacactt caggggaaag gtggtatctc
     1261 taagcaagag aactgagtgg agtcaaggct gagagatgca ggataagcaa atgggtagtg
     1321 aaaagacatt catgaggaca gctaaaacaa taagtaatgt aaaatacagc atagcaaaac
     1381 tttaacctcc aaatcaagcc tctacttgaa tccttttctg agggatgaat aaggcatagg
     1441 catcaggggc tgttgccaat gtgcattagc tgtttgcagc ctcaccttct ttcatggagt
     1501 ttaagatata gtgtattttc ccaaggtttg aactagctct tcatttcttt atgttttaaa
     1561 tgcactgacc tcccacattc cctttttagt aaaatattca gaaataattt aaatacatca
     1621 ttgcaatgaa aataaatgtt ttttattagg cagaatccag atgctcaagg cccttcataa
     1681 tatcccccag tttagtagtt ggacttaggg aacaaaggaa cctttaatag aaattggaca
     1741 gcaagaaagc gagcttagtg atacttgtgg gccagggcat tagccacacc agccaccact
     1801 ttctgatagg cagcctgcac tggtggggtg aattccgttt tttttttttt ttttcataaa
     1861 aattaaaaac tcaaatataa ttgaggcctc tttgagcatg gtatcacaag ttgatttggt
     1921 ccaaacatga agaatctgtt gtgcaggatt tgagttactt tccaagtcgg ttcatctcta
     1981 tgtctgtata aatctgtctt ttcttggtgt gctttaattt aatgcaaaga tggataccaa
     2041 ctcggagaac caagaatagt ccaatgatta accctatgat aaagaaaaaa gaggcaatag
     2101 agcttttcca actactgaac caaccttcta caagctcgat tggatttttg gatagcccag
     2161 tatcaccaaa aaataaactc tcatcatcag gaagttgcga agcagcgtct tgaatgtgag
     2221 gatgttcgaa cacctgagcc tttgagctaa gatgaagatc ggagtccaac ataccatgtc
     2281 caatcatgta taaaggaaac ttatatcctg aactggtcct cagaactcca ttgggtccaa
     2341 tttccacgtc ttcatatggt gcccagtcat cccacagttc cctttctgtg gtagttccac
     2401 tgatcattcc gaccattctt gagaggattg gagcagcaat atcgactctg atgtatctgg
     2461 tctcaaagta ttttagggta ccattgatta tggtgaaagc aggaccggtt cctgggtttt
     2521 taggagcaag atagctgaga tccactggag agattggaag acccgctctg attttgctcc
     2581 aggtttcttg gcagagggaa taatccaaga tcctctcaac gtcctgaatt agacttacat
     2641 ccactgaggt ctgagatgga gcagagatac ttgacccttc tgggcattca gggaatctgg
     2701 ctgcagcaaa gagatcctta tcagccatct cgaaccagac acctgatggg agtctgactc
     2761 cccaatgctt gcagtattgc attttgcagg ccttgcctcc agtttcataa gcaaagtagt
     2821 tacttctgaa ccctgtgccc tcctttccca gggatgatag ctctccgtcc tctgagaaga
     2881 aggtgatgtc catggaaatg aggttagaat cacatagccc tttgacctta tagtcagaat
     2941 gccaggttgt agagttatgg acagtggggc atatgtaatt gctgcatttt ccgttgatga
     3001 actgtgaatc aacccattct cctgtgtatt catcaaccag cacatggtga ggagtcacct
     3061 ggacaatcac tgcttcggca tccgtcacag ttgcatatcc acaactttga ggagggaagc
     3121 ctggattcag ccaagttcct tgtttcgttt gttcaatgct ttccttgcat tgttctacag
     3181 atggagtgaa ggatcggatg gaatgtgtta tatacttcgg tccataccag cggaaatcac
     3241 aagtagtgac ccatttggaa gcatgacaca tccaaccgtc tgcttgaata gccttgtgac
     3301 tcttgggcat tttgacttgt aaggctgtgc ctattaagtc attatgccaa tttaaatctg
     3361 agcttgacgg gcaataatgg taattagaag gaacattttt ccagtttcct ttttggttgt
     3421 gtggaaaaac tatggtgaac ttgcaattca ccccaatgaa taaaaaggct aagtacaaaa
     3481 ggcacttcat agtgtcagaa ttcagatctc acgtgctttg ccaaagtgat gggccagcac
     3541 acagaccagc acgttgccca ggagctgtgg gaggaagata agaggtatga acatgattag
     3601 caaaagggcc tagcttggac tcagaataat ccagccttat cccaaccata aaataaaagc
     3661 agaatggtag ctggattgta gctgctatta gcaatatgaa acctcttaca tcagttacaa
     3721 tttatatgca gaaatattta tatgcagaaa tattgctatt gccttaaccc agaaattatc
     3781 actgttattc tttagaatgg tgcaaagagg catgatacat tgtatcatta ttgccctgaa
     3841 agaaagagat tagggaaagt attagaaata agataaacaa aaaagtatat taaaagaaga
     3901 aagcattttt taaaattaca aatgcaaaat taccctgatt tggtcaatat gtgtaccctg
     3961 ttacttctcc ccttcctatg acatgaactt aaccatagaa aagaagggga aagaaaacat
     4021 caagggtccc atagactcac cctgaagttc tcaggatccg agctcggtac cacatgtaag
     4081 cttcgagggg aggctggatc ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg
     4141 gcgtctccag gcgatctgac ggttcactaa acgagctctg cttatataga cctcccaccg
     4201 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
     4261 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     4321 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
     4381 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     4441 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     4501 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     4561 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     4621 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     4681 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     4741 agtggatccg agctcggtac caagcttgat gcatagcttg agtattctaa cgcgtcacct
     4801 aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4861 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4921 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4981 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5041 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5101 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5161 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5221 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5281 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5341 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5401 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5461 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5521 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5581 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5641 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5701 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5761 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5821 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5881 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5941 tagcacgtgt cagtcctgct cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg
     6001 cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc
     6061 gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg
     6121 cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa
     6181 cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa
     6241 cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg
     6301 aacggcactg gtcaacttgg ccatggtggc cctcctcacg tgctattatt gaagcattta
     6361 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6421 aggggttccg cgcacatttc cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca
     6481 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaataatt cagaagaact
     6541 cgagaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     6601 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     6661 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     6721 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
     6781 cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     6841 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     6901 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     6961 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     7021 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     7081 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     7141 cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     7201 gcgctgacag ccggaactcg agggcatcag agcagccgat tgtctgttgt gcccagtcat
     7261 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     7321 tcatgcgaaa cgatcctcat cctgtctctt gatcagagct tgatcccctg cgccatcaga
     7381 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
     7441 gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc
     7501 gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc
     7561 agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
     7621 acgtgaaaag gatctaggtg aagatccttt ttgataatct catgcctgac atttatattc
     7681 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     7741 cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc
     7801 tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac
     7861 gtgcactggc cagggggatc accatccgtc gccccggcgt gtcaataata tcactctgta
     7921 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgccgta
     7981 cgtataggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     8041 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     8101 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
     8161 tgggccctct agagatatcc tcgagcaaca tgaagtcagg atgggacagg atgtatatat
     8221 ctgacacgtg cctggagact agggagtaat ccccttggcg gttaaaacgc gggggacagc
     8281 gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg cctcggcacc
     8341 gggattctcc agttacatga tcaccagaat tctctagata tgttttatga cataaacgac
     8401 aggatgtata tatctgacac gtgcctggag actagggagt aatccccttg gcggttaaaa
     8461 cgcgggggac agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag
     8521 cggcctcggc accgggattc tccagatctt cgaacatttg aattccggcc gtttcaggta
     8581 gcttgctaag aacaggatgt atatatctga cacgtgcctg gagactaggg agtaatcccc
     8641 ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct agagctgtct
     8701 acgaccaatt gagcggcctc ggcaccggga ttctccagaa atcaactttg aggaattcga
     8761 cgtcaaagtc tttactatat gcaaacagga tgtatatatc tgacacgtgc ctggagacta
     8821 gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt
     8881 gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca gttctcgaaa
     8941 caccggaatt cggtaccata tccaaaaccg ctccttcaca ggatgtatat atctgacacg
     9001 tgcctggaga ctagggagta atccccttgg cggttaaaac gcgggggaca gcgcgtacgt
     9061 gcgtttaagc ggtgctagag ctgtctacga ccaattgagc ggcctcggca ccgggattct
     9121 ccagagcatt gaaatctcga attccccggg ctcttttgga caaccaccca acaggatgta
     9181 tatatctgac acgtgcctgg agactaggga gtaatcccct tggcggttaa aacgcggggg
     9241 acagcgcgta cgtgcgttta agcggtgcta gagctgtcta cgaccaattg agcggcctcg
     9301 gcaccgggat tctccagaca tgtgaattcg agctctgtgt gatctctatc actgataggg
     9361 aacacaacag gatgtatata tctgacacgt gcctggagac tagggagtaa tccccttggc
     9421 ggttaaaacg cgggggacag cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac
     9481 caattgagcg gcctcggcac cgggattctc cagatgatgg ccgaattcct gcagtgtgtt
     9541 aatttaacat caccacagga tgtatatatc tgacacgtgc ctggagacta gggagtaatc
     9601 cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt gctagagctg
     9661 tctacgacca attgagcggc ctcggcaccg ggattctcca gatcaatata ataaggaatt
     9721 cccatggtgg gaaactccaa acccaataca ggatgtatat atctgacacg tgcctggaga
     9781 ctagggagta atccccttgg cggttaaaac gcgggggaca gcgcgta
//
