LOCUS       sequence-357807-       10195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP driven by 453bp enhancer of Mhc gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: pBac{Mhc-453-GFP,attB,w+}
FEATURES             Location/Qualifiers
     source          1..10195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        727..965
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     912..933
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          980..1046
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             1077..1790
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          1877..1942
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2072..2092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2364..2498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2401..2420)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2455..2474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   2785..2819
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2855..2873)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2940..3044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3045..3113,3114..3905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3263..3282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4076..4664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4565..4584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4731..4793
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    5789..5858
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(6083..6101)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6553..6624,7026..7299,7374..8028,8090..8405,8609..8740,
                     8811..9425)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 cgcgcctccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa
       61 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
      121 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc
      181 agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc cagggccgca
      241 catatgcaat gacgtaacaa ataccaacgc cccgaaaatc cgtatatatg caaagcattc
      301 tgatatatat atatataagt ggtatacata tacatattcg tatcggatcc gtgatctata
      361 tttaggcgac aacccacaca attcacttgt tgtctgttta taacaaaaac aacaacacaa
      421 gcaccgacca ggcctaatta actttgaggt gtgagataaa atcaaagcca cacataaata
      481 aatatccggt tacggtaacg ggaatttcga aagcgcgaaa cgggaaaaat gttcatattt
      541 ggaaatcgaa attaaatttc gaaatcctat ccacgcacac acgcgtagaa ctgttatagt
      601 gtccgctatt gctgctgctg tcaaaatggg tcaaaagcat aaaatccata aaagaatcag
      661 acttcgaaac aaaattccaa aaatagtatg cttttctgag aaccatcgat ttcgaaccct
      721 cgaccgccgg agtataaata gaggcgcttc gtctacggag cgacaattca attcaaacaa
      781 gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc
      841 taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa
      901 gtaaatcaac tgcaactact gaaatctgcc aagaagtaat tattgaatac aagaagagaa
      961 ctctgaatag atctaaaagg taggttcaac cactgatgcc taggcacacc gaaacgacta
     1021 accctaattc ttatccttta cttcaggcgg ccgcggctcg agggtaccaa tcaaacatgt
     1081 ccaaaggtga agaactgttt accggagtag tcccgatatt ggttgaactc gacggcgatg
     1141 tcaacggtca taaattcagt gtgtccggcg agggtgaggg cgacgccaca tacggtaagc
     1201 tgacgttgaa gttcatatgc accacgggca agctgcccgt gccatggccg acgttggtca
     1261 cgacgctgac gtatggtgtc cagtgtttca gccgttaccc cgatcatatg aagcagcatg
     1321 actttttcaa gtcggcgatg ccggagggat acgttcaaga gaggaccatt ttcttcaagg
     1381 atgacggcaa ctataagacg cgagcggagg tgaaatttga aggcgacaca ctcgttaacc
     1441 gtattgagtt gaagggcatt gattttaagg aggatggcaa cattctgggc cataagttgg
     1501 aatacaacta caactcgcat aatgtgtata taatggcaga taagcagaaa aacggaataa
     1561 aggttaactt caagattcgc cacaacatag aggacggttc cgtgcaactt gcagatcatt
     1621 accaacagaa cacacccatt ggagatggcc cagttctctt gccagacaat cactaccttt
     1681 ccacacagtc cgcgttgagc aaggacccca atgaaaagcg ggaccacatg gtgttgctgg
     1741 agtttgtgac cgcagctggt attacacacg gcatggatga gctctacaag taatctagag
     1801 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
     1861 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
     1921 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
     1981 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
     2041 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
     2101 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
     2161 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
     2221 tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     2281 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2341 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2401 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2461 tgtccaaact catcaatgta tcttatcatg tctggatcga tctggccggc gatatctata
     2521 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
     2581 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
     2641 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
     2701 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     2761 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggg
     2821 ccggccgttt aaacgaattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     2881 gttaatgtca tgataataat ggtttcttag actcaggtgg cacttttcgg ggaaatgtgc
     2941 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3001 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3061 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3121 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3181 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3241 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3301 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3361 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3421 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3481 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3541 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     3601 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3661 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3721 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3781 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3841 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3901 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3961 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4021 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4081 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4141 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4201 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4261 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4321 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4381 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4441 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4501 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4561 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4621 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4681 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttaccg ccctagaaag
     4741 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
     4801 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
     4861 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
     4921 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
     4981 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     5041 aattagctta acttatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     5101 tttggcacat atcaatatta tgctctcgac aaataacttt tttgcatttt ttgcacgatg
     5161 catttgcctt tcgccttatt ttagaggggc agtaagtaca gtaagtacgt tttttcatta
     5221 ctggctcttc agtactgtca tccgatgtac caggcacttc atttggcaaa atattagaga
     5281 tattatcgcg caaatatctc ttcaaagtag gagcttctaa acgcttacgc ataaacgatg
     5341 acgtcaggct catgtaaagg tttctcataa attttttgcg actttgaacc ttttctccct
     5401 tgctactgac attatggctg tatataataa aagaatttat gcaggcaatg tttatcattc
     5461 cgtacaataa tgccataggc cacctattcg tcttcctact gcaggtcatc acagaacaca
     5521 tttggtctag cgtgtccact ccgcctttag tttgattata atacataacc atttgcggtt
     5581 taccggtact ttcgttgata gaagcatcct catcacaaga tgataataag tataccatct
     5641 tagctggctt cggtttatat gagacgagag taaggggtcc gtcaaaacaa aacatcgatg
     5701 ttcccactgg cctggagcga ctgtttttca gtacttccgg tatctcgcgt ttgtttgatc
     5761 gcacggttcc cacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
     5821 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt
     5881 cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga
     5941 aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg
     6001 atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg acaagccgaa
     6061 catatgggcg cgcctagtat gtatgtaagt taataaaacc catttttgcg gaaagtagat
     6121 aaaaaaaaca tttttttttt ttactgcact ggatatcatt gaacttatct gatcagtttt
     6181 aaatttactt cgatccaagg gtatttgatg taccaggttc tttcgattac ctctcactca
     6241 aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg
     6301 ccgtctcttt cgccgctgcg tccgctatct ctttcgccac cgtttgtagc gttacgtagc
     6361 gtcaatgtcc gccttcagtt gcattttgtc agcggtttcg tgacgaagct ccaagcggtt
     6421 tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt
     6481 tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga
     6541 aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac
     6601 ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt
     6661 gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac
     6721 aaaaatccca aaccgcaatt gcacaaacaa atagtgacac gaaacagatt attctggtag
     6781 ctgttctcgc tatataagac aatttttgag atcatatcat gatcaagaca tctaaaggca
     6841 ttcattttcg actatattct tttttacaaa aaatataaca accagatatt ttaagctgat
     6901 cctagatgca caaaaaataa ataaaagtat aaacctactt cgtaggatac ttcggggtac
     6961 tttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat
     7021 tgcagggtga cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca
     7081 aaaactacgg cacgctccgg ccacccagtc cgccggagga ctccggttca gggagcggcc
     7141 aactagccga gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc
     7201 agccgggctc cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc
     7261 gacacatacc ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg
     7321 gctgatctgt gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg
     7381 cgtggcctat ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac
     7441 cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat
     7501 gcgactgctc aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt
     7561 ccagcaggat gacctcttta tcggctccct aacggccagg gaacacctga ttttccaagc
     7621 catggtgcgg atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt
     7681 gatccaggag ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt
     7741 gaaaggtctg tccggcggag aaaggaagcg tctggcattc gcctccgagg ctctaaccga
     7801 tccgccgctt ctgatctgcg atgagcccac ctccggactg gactccttta ccgcccacag
     7861 cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca
     7921 tcagccgtct tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag
     7981 ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg
     8041 tgtttattaa gggtatctag tattacataa catctcaact cctatccagc gtgggtgccc
     8101 agtgtcctac caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg
     8161 gacgggagat cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gccattagca
     8221 aagtagcccg ggatatggag cagttgttgg ccaccaaaaa tctggagaag ccactggagc
     8281 agccggagaa tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt
     8341 ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga
     8401 caacggtgag tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa
     8461 ttcgctagat tttagataga attgcctgat tccacaccct tcttagtttt tttcaatgag
     8521 atgtatagtt tatagttttg cagaagataa ataaatttca tttaactcgc gaatattaat
     8581 gagatgcgag taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt
     8641 gggccaacaa ctcacgcaag tgggtgtgat gaatatcaac ggagccatct tcctcttcct
     8701 gaccaacatg acctttcaaa acgtctttgc cacgataaat gtaagtcatg tttagaatac
     8761 atttgcattt caataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct
     8821 cagagctgcc agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact
     8881 ttctgggcaa aacgattgcc gaattgccgc tttttctcac agtgccactg gtcttcacgg
     8941 cgattgccta tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg
     9001 cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca
     9061 gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct
     9121 ttggcggctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc
     9181 tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc
     9241 cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc
     9301 tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc
     9361 tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg
     9421 agtagccgac atatatccga aataactgct tgtttttttt ttttaccatt attaccatcg
     9481 tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac
     9541 aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg
     9601 gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta
     9661 tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct
     9721 cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc
     9781 gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg
     9841 cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta
     9901 ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag
     9961 ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg
    10021 agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat
    10081 gcatctcaaa aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta
    10141 taataataag aatacattta atttagaaaa tgcttggatt tcactggaac taggg
//
