LOCUS       sequence-357809-        3569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector of TAZ S89A for gateway coloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Wang H, Zhang S, Zhang Y, Jia J, Wang J, Liu X, Zhang J, Song X, 
            Ribback S, Cigliano A, Evert M, Liang B, Wu H, Calvisi DF, Zeng Y, 
            Chen X
  TITLE     TAZ is indispensable for c-MYC-induced hepatocarcinogenesis.
  JOURNAL   J Hepatol. 2021 Aug 28. pii: S0168-8278(21)02016-X. doi: 
            10.1016/j.jhep.2021.08.021.
  PUBMED    34464659
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Hepatol. 
            2021 Aug 28. pii: S0168-8278(21)02016-X. doi: 
            10.1016/j.jhep.2021.08.021."
COMMENT     Sequence Label: pENTR1A-TAZ S89A
FEATURES             Location/Qualifiers
     source          1..3569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      249..335
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      427..454
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     462..485
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    504..603
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             643..708
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1944..2043)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2155..2174)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2166..2975
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2233..2252)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3065..3569,1..84)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     join(3554..3569,1..4)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
       61 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      121 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      181 cgtattaccg ctagcatgga tctcggggac gtctaactac taagcgagag tagggaactg
      241 ccaggcatca aataaaacga aaggctcagt cggaagactg ggcctttcgt tttatctgtt
      301 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
      361 tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
      421 ctaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttcctgtta
      481 gttagttact taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt
      541 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagcag
      601 gctttaaagg aaccaattca gtcgactgga tccggtacca tggactacaa agaccatgac
      661 ggtgattata aagatcatga catcgattac aaggatgacg atgacaagct tgggaatccg
      721 gcctcggcgc cccctccgct cccgccgcct gggcagcaag tgatccacgt cacgcaggac
      781 ctagacacag acctcgaagc cctcttcaac tctgtcatga atccgaagcc tagctcgtgg
      841 cggaagaaga tcctgccgga gtctttcttt aaggagcctg attcgggctc gcactcgcgc
      901 cagtccagca ccgactcgtc gggcggccac ccggggcctc gactggctgg gggtgcccag
      961 catgtccgct cgcacgcctc gccagcgtcc ctgcagctgg gcaccggcgc gggtgctgcg
     1021 ggtagccccg cgcagcagca cgcgcacctc cgccagcagt cctacgacgt gaccgacgag
     1081 ctgccactgc ccccgggctg ggagatgacc ttcacggcca ctggccagag gtacttcctc
     1141 aatcacatag aaaaaatcac cacatggcaa gaccctagga aggcgatgaa tcagcctctg
     1201 aatcatatga acctccaccc tgccgtcagt tccacaccag tgcctcagag gtccatggca
     1261 gtatcccagc caaatctcgt gatgaatcac caacaccagc agcagatggc ccccagtacc
     1321 ctgagccagc agaaccaccc cactcagaac ccacccgcag ggctcatgag tatgcccaat
     1381 gcgctgacca ctcagcagca gcagcagcag aaactgcggc ttcagagaat ccagatggag
     1441 agagaaagga ttcgaatgcg ccaagaggag ctcatgaggc aggaagctgc cctctgtcga
     1501 cagctcccca tggaagctga gactcttgcc ccagttcagg ctgctgtcaa cccacccacg
     1561 atgaccccag acatgagatc catcactaat aatagctcag atcctttcct caatggaggg
     1621 ccatatcatt cgagggagca gagcactgac agtggcctgg ggttagggtg ctacagtgtc
     1681 cccacaactc cggaggactt cctcagcaat gtggatgaga tggatacagg agaaaacgca
     1741 ggacaaacac ccatgaacat caatccccaa cagacccgtt tccctgattt ccttgactgt
     1801 cttccaggaa caaacgttga cttaggaact ttggaatctg aagacctgat ccccctcttc
     1861 aatgatgtag agtctgctct gaacaaaagt gagccctttc taacctggct gtaagaattc
     1921 gcggccgcac tcgagatatc tagacccagc tttcttgtac aaagttggca ttataagaaa
     1981 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     2041 ttgccatcca gctgcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     2101 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     2161 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
     2221 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     2281 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     2341 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     2401 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     2461 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     2521 ttgatgcgct ggcagtgtcc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     2581 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     2641 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     2701 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     2761 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     2821 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     2881 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     2941 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     3001 attattcaga ttgggccccg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3061 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3121 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3181 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3241 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3301 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3361 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3421 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3481 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3541 gggagcttcc agggggaaac gcctggtat
//
