LOCUS       sequence-35781-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Cronin JC, Wunderlich J, Loftus SK, Prickett TD, Wei X, Ridd K, 
            Vemula S, Burrell AS, Agrawal NS, Lin JC, Banister CE, Buckhaults P,
            Rosenberg SA, Bastian BC, Pavan WJ, Samuels Y
  TITLE     Frequent mutations in the MITF pathway in melanoma.
  JOURNAL   Pigment Cell Melanoma Res. 2009 Aug . 22(4):435-44.
  PUBMED    19422606
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pigment 
            Cell Melanoma Res. 2009 Aug . 22(4):435-44."
COMMENT     Sequence Label: pCMV-Tag4A-MITF-M (L135V)
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..35)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     37..53
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     38..54
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 accgggccct acttatcgtc gtcatccttg taatcctcga ggtcgacggt atcgataagc
       61 ttacaagtgt gctccgtctc ttccatgctc atactgctcc tccggctgct tgttttggaa
      121 gctccggggg acactgagga aaggagtgga tcagtgacac cgacgggaga aagggtgtcg
      181 tccatcagga tgtcttccag tttggatccc atttttgtgg ggacactata ggcttggttg
      241 gcctcagtcc cagttccgag gttgttgttg aaggtgatgg tgccatccgt gagatcgaga
      301 gttgttgtac aggttaggtc tgcatgatgc tgaaggaggt cttggctgca gttctcaaga
      361 acgggttctt gcttgatgat ccgattcacc aaatctggag agcagagacc cgtggatgga
      421 ataagggaaa gtccatgagc tcgagcctgc atttcaagtt cctgtattct gagcaacaaa
      481 tgccggttgg cgtgctccag tttcttctgt cggttttcaa gttcttttgc gcgttgctgt
      541 tctcgttgca actttcggat atagtccacg gatgctttta agatggttcc cttgttccag
      601 cgcatgtctg gatcatttga cttgggaatc aaagtaccta gttctttaat gcggtcattt
      661 atgttaaatc ttcttcttcg ttcaatcagg ttgtgattgt cctttttctg cctctctttg
      721 gccagtgctc ttgcttcaga ctctgtgagc tcccttttta tgttgggaag gttggctgga
      781 caggagttgc tgatggtgag gcctggtggg ggcagacctt ggtttccata aagatcaatc
      841 aagtttcccg agacaggcaa cgtatttgcc atttgcaaag canatccatc aagcccacga
      901 tttcctcatt ataacttgat tctaggctaa tgatgtcatc gattacatca tccatctgca
//
