LOCUS       sequence-357811-       10026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP driven by Act88F enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 10026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: pBac{Act88F-eGFP,attB,w+}
FEATURES             Location/Qualifiers
     source          1..10026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        831..1069
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     1016..1037
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          1084..1150
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             1181..1894
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          1981..2046
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2176..2196
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2468..2602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2505..2524)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2559..2578
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   2889..2923
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2959..2977)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3044..3148
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3149..3217,3218..4009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3367..3386)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4180..4768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4669..4688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4835..4897
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    5893..5962
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(6187..6205)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6657..6728,7130..7403,7478..8132,8194..8509,8713..8844,
                     8915..9529)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 tatttgctcc aatatatttg tatatatttc cctcacagct atatttattc taatttaata
       61 ttatgacttt ttaaggtaat tttttgtgac ctgttcggag tgattagcgt tacaatttga
      121 actgaaagtg acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc
      181 ataatagtgt tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat
      241 ttagaaaatg cttggatttc actggaacta gggcgcgcct ccggaacata atggtgcagg
      301 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg
      361 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
      421 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
      481 gcacgatcat gcgcacccgt ggccagggcc gcacatatgc aatgctggca atggttggtt
      541 aattgcactg ataaatggtc ggcacggtga tttcgcatct tcgggattgc atcggcgccg
      601 caatgcaaag tgcagcagca ttctgtagaa tgcgattgca aatgtggatg cagcttcctc
      661 gagcaccgcg cgcggagatc tcgatcaacc ttgatgttga tttataggtg ccgctctgct
      721 tggcgcgtct attttagatt cgcctcgctg cgtgcccgtt gaaatgtccc attctcccag
      781 tccctgccgc ggatgccaat tgtcttgcat cgatttcgaa ccctcgaccg ccggagtata
      841 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
      901 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
      961 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
     1021 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagatctaa
     1081 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc
     1141 tttacttcag gcggccgcgg ctcgagggta ccaatcaaac atgtccaaag gtgaagaact
     1201 gtttaccgga gtagtcccga tattggttga actcgacggc gatgtcaacg gtcataaatt
     1261 cagtgtgtcc ggcgagggtg agggcgacgc cacatacggt aagctgacgt tgaagttcat
     1321 atgcaccacg ggcaagctgc ccgtgccatg gccgacgttg gtcacgacgc tgacgtatgg
     1381 tgtccagtgt ttcagccgtt accccgatca tatgaagcag catgactttt tcaagtcggc
     1441 gatgccggag ggatacgttc aagagaggac cattttcttc aaggatgacg gcaactataa
     1501 gacgcgagcg gaggtgaaat ttgaaggcga cacactcgtt aaccgtattg agttgaaggg
     1561 cattgatttt aaggaggatg gcaacattct gggccataag ttggaataca actacaactc
     1621 gcataatgtg tatataatgg cagataagca gaaaaacgga ataaaggtta acttcaagat
     1681 tcgccacaac atagaggacg gttccgtgca acttgcagat cattaccaac agaacacacc
     1741 cattggagat ggcccagttc tcttgccaga caatcactac ctttccacac agtccgcgtt
     1801 gagcaaggac cccaatgaaa agcgggacca catggtgttg ctggagtttg tgaccgcagc
     1861 tggtattaca cacggcatgg atgagctcta caagtaatct agaggatctt tgtgaaggaa
     1921 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     1981 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     2041 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     2101 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     2161 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     2221 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     2281 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatatttg
     2341 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     2401 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2461 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2581 tgtatcttat catgtctgga tcgatctggc cggcgatatc tataacaaga aaatatatat
     2641 ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa
     2701 tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt
     2761 caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac
     2821 tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt
     2881 caagaatgca tgcgtcaatt ttacgcagac tatctttcta ggggccggcc gtttaaacga
     2941 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     3001 taatggtttc ttagactcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3061 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3121 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3181 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3241 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3301 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3361 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3421 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3481 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3541 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3601 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3661 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3721 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3781 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3841 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3901 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3961 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4021 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4081 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4141 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4201 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4261 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4321 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4381 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4441 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4501 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4561 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4621 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4681 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4741 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4801 ggccttttgc tggccttttg ctcacatgtt accgccctag aaagataatc atattgtgac
     4861 gtacgttaaa gataatcatg cgtaaaattg acgcatgtgt tttatcggtc tgtatatcga
     4921 ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt acatactaat
     4981 aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc
     5041 aaaatttctt ctataaagta acaaaacttt taaacattct ctcttttaca aaaataaact
     5101 tattttgtac tttaaaaaca gtcatgttgt attataaaat aagtaattag cttaacttat
     5161 acataataga aacaaattat acttattagt cagtcagaaa caactttggc acatatcaat
     5221 attatgctct cgacaaataa cttttttgca ttttttgcac gatgcatttg cctttcgcct
     5281 tattttagag gggcagtaag tacagtaagt acgttttttc attactggct cttcagtact
     5341 gtcatccgat gtaccaggca cttcatttgg caaaatatta gagatattat cgcgcaaata
     5401 tctcttcaaa gtaggagctt ctaaacgctt acgcataaac gatgacgtca ggctcatgta
     5461 aaggtttctc ataaattttt tgcgactttg aaccttttct cccttgctac tgacattatg
     5521 gctgtatata ataaaagaat ttatgcaggc aatgtttatc attccgtaca ataatgccat
     5581 aggccaccta ttcgtcttcc tactgcaggt catcacagaa cacatttggt ctagcgtgtc
     5641 cactccgcct ttagtttgat tataatacat aaccatttgc ggtttaccgg tactttcgtt
     5701 gatagaagca tcctcatcac aagatgataa taagtatacc atcttagctg gcttcggttt
     5761 atatgagacg agagtaaggg gtccgtcaaa acaaaacatc gatgttccca ctggcctgga
     5821 gcgactgttt ttcagtactt ccggtatctc gcgtttgttt gatcgcacgg ttcccacgat
     5881 gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg
     5941 cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt
     6001 gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt
     6061 ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt
     6121 cagcgggttc tcgacggtca cggcgggcat gtcgacaagc cgaacatatg ggcgcgccta
     6181 gtatgtatgt aagttaataa aacccatttt tgcggaaagt agataaaaaa aacatttttt
     6241 ttttttactg cactggatat cattgaactt atctgatcag ttttaaattt acttcgatcc
     6301 aagggtattt gatgtaccag gttctttcga ttacctctca ctcaaaatga cattccactc
     6361 aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat atcgccgtct ctttcgccgc
     6421 tgcgtccgct atctctttcg ccaccgtttg tagcgttacg tagcgtcaat gtccgccttc
     6481 agttgcattt tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc atcaattaaa
     6541 cacaaagtgc tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt
     6601 ggggtggtga ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg
     6661 gccaagagga tcaggagcta ttaattcgcg gaggcagcaa acacccatct gccgagcatc
     6721 tgaacaatgt gagtagtaca tgtgcataca tcttaagttc acttgatcta taggaactgc
     6781 gattgcaaca tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc
     6841 aattgcacaa acaaatagtg acacgaaaca gattattctg gtagctgttc tcgctatata
     6901 agacaatttt tgagatcata tcatgatcaa gacatctaaa ggcattcatt ttcgactata
     6961 ttctttttta caaaaaatat aacaaccaga tattttaagc tgatcctaga tgcacaaaaa
     7021 ataaataaaa gtataaacct acttcgtagg atacttcggg gtactttttg ttcggggtta
     7081 gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg gtgacagcgg
     7141 agcggcttcg cagagctgca ttaaccaggg cttcgggcag gccaaaaact acggcacgct
     7201 ccggccaccc agtccgccgg aggactccgg ttcagggagc ggccaactag ccgagaacct
     7261 cacctatgcc tggcacaata tggacatctt tggggcggtc aatcagccgg gctccggatg
     7321 gcggcagctg gtcaaccgga cacgcggact attctgcaac gagcgacaca taccggcgcc
     7381 caggaaacat ttgctcaaga acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa
     7441 tcttaataaa gggtccaatt accaatttga aactcagttt gcggcgtggc ctatccgggc
     7501 gaacttttgg ccgtgatggg cagttccggt gccggaaaga cgaccctgct gaatgccctt
     7561 gcctttcgat cgccgcaggg catccaagta tcgccatccg ggatgcgact gctcaatggc
     7621 caacctgtgg acgccaagga gatgcaggcc aggtgcgcct atgtccagca ggatgacctc
     7681 tttatcggct ccctaacggc cagggaacac ctgattttcc aagccatggt gcggatgcca
     7741 cgacatctga cctatcggca gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg
     7801 ctcagcaaat gtcagcacac gatcatcggt gtgcccggca gggtgaaagg tctgtccggc
     7861 ggagaaagga agcgtctggc attcgcctcc gaggctctaa ccgatccgcc gcttctgatc
     7921 tgcgatgagc ccacctccgg actggactcc tttaccgccc acagcgtcgt ccaggtgctg
     7981 aagaagctgt cgcagaaggg caagaccgtc atcctgacca ttcatcagcc gtcttccgag
     8041 ctgtttgagc tctttgacaa gatccttctg atggccgagg gcagggtagc tttcttgggc
     8101 actcccagcg aagccgtcga cttcttttcc tagtgagttc gatgtgttta ttaagggtat
     8161 ctagtattac ataacatctc aactcctatc cagcgtgggt gcccagtgtc ctaccaacta
     8221 caatccggcg gacttttacg tacaggtgtt ggccgttgtg cccggacggg agatcgagtc
     8281 ccgtgatcgg atcgccaaga tatgcgacaa ttttgccatt agcaaagtag cccgggatat
     8341 ggagcagttg ttggccacca aaaatctgga gaagccactg gagcagccgg agaatgggta
     8401 cacctacaag gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc
     8461 ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc
     8521 cagtggaaac aaatgatata acgcttacaa ttcttggaaa caaattcgct agattttaga
     8581 tagaattgcc tgattccaca cccttcttag tttttttcaa tgagatgtat agtttatagt
     8641 tttgcagaag ataaataaat ttcatttaac tcgcgaatat taatgagatg cgagtaacat
     8701 tttaatttgc agatggttgc catcttgatt ggcctcatct ttttgggcca acaactcacg
     8761 caagtgggtg tgatgaatat caacggagcc atcttcctct tcctgaccaa catgaccttt
     8821 caaaacgtct ttgccacgat aaatgtaagt catgtttaga atacatttgc atttcaataa
     8881 tttactaact ttctaatgaa tcgattcgat ttaggtgttc acctcagagc tgccagtttt
     8941 tatgagggag gcccgaagtc gactttatcg ctgtgacaca tactttctgg gcaaaacgat
     9001 tgccgaattg ccgctttttc tcacagtgcc actggtcttc acggcgattg cctatccgat
     9061 gatcggactg cgggccggag tgctgcactt cttcaactgc ctggcgctgg tcactctggt
     9121 ggccaatgtg tcaacgtcct tcggatatct aatatcctgc gccagctcct cgacctcgat
     9181 ggcgctgtct gtgggtccgc cggttatcat accattcctg ctctttggcg gcttcttctt
     9241 gaactcgggc tcggtgccag tatacctcaa atggttgtcg tacctctcat ggttccgtta
     9301 cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg aaattagctg
     9361 cacatcgtcg aacaccacgt gccccagttc gggcaaggtc atcctggaga cgcttaactt
     9421 ctccgccgcc gatctgccgc tggactacgt gggtctggcc attctcatcg tgagcttccg
     9481 ggtgctcgca tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat
     9541 ccgaaataac tgcttgtttt ttttttttac cattattacc atcgtgttta ctgtttattg
     9601 ccccctcaaa aagctaatgt aattatattt gtgccaataa aaacaagata tgacctatag
     9661 aatacaagta tttccccttc gaacatcccc acaagtagac tttggatttg tcttctaacc
     9721 aaaagactta cacacctgca taccttacat caaaaactcg tttatcgcta cataaaacac
     9781 cgggatatat tttttatata catacttttc aaatcgcgcg ccctcttcat aattcacctc
     9841 caccacacca cgtttcgtag ttgctctttc gctgtctccc acccgctctc cgcaacacat
     9901 tcaccttttg ttcgacgacc ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct
     9961 caaatggttc cgagtggttc atttcgtctc aatagaaatt agtaataaat atttgtatgt
    10021 acaatt
//
