LOCUS       sequence-357812-        9902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP driven by tinman transcription factor binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 9902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: pBac{9Xtin-eGFP,w+,attB}
FEATURES             Location/Qualifiers
     source          1..9902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    987..1056
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(1281..1299)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1751..1822,2224..2497,2572..3226,3288..3603,3807..3938,
                     4009..4623)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        5827..6065
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6012..6033
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          6080..6146
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             6177..6890
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          6977..7042
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7172..7192
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7464..7598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7501..7520)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7555..7574
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7885..7919
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7955..7973)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8040..8144
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8145..8213,8214..9005)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8363..8382)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9176..9764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9665..9684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   9831..9893
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ggtctgtata tcgaggttta tttattaatt tgaatagata ttaagtttta ttatatttac
       61 acttacatac taataataaa ttcaacaaac aatttattta tgtttattta tttattaaaa
      121 aaaaacaaaa actcaaaatt tcttctataa agtaacaaaa cttttaaaca ttctctcttt
      181 tacaaaaata aacttatttt gtactttaaa aacagtcatg ttgtattata aaataagtaa
      241 ttagcttaac ttatacataa tagaaacaaa ttatacttat tagtcagtca gaaacaactt
      301 tggcacatat caatattatg ctctcgacaa ataacttttt tgcatttttt gcacgatgca
      361 tttgcctttc gccttatttt agaggggcag taagtacagt aagtacgttt tttcattact
      421 ggctcttcag tactgtcatc cgatgtacca ggcacttcat ttggcaaaat attagagata
      481 ttatcgcgca aatatctctt caaagtagga gcttctaaac gcttacgcat aaacgatgac
      541 gtcaggctca tgtaaaggtt tctcataaat tttttgcgac tttgaacctt ttctcccttg
      601 ctactgacat tatggctgta tataataaaa gaatttatgc aggcaatgtt tatcattccg
      661 tacaataatg ccataggcca cctattcgtc ttcctactgc aggtcatcac agaacacatt
      721 tggtctagcg tgtccactcc gcctttagtt tgattataat acataaccat ttgcggttta
      781 ccggtacttt cgttgataga agcatcctca tcacaagatg ataataagta taccatctta
      841 gctggcttcg gtttatatga gacgagagta aggggtccgt caaaacaaaa catcgatgtt
      901 cccactggcc tggagcgact gtttttcagt acttccggta tctcgcgttt gtttgatcgc
      961 acggttccca cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc
     1021 ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat gaacgggtcg
     1081 aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg ggaagccctc gccctcgaaa
     1141 ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga cggcgtcggc gggtgcggat
     1201 acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg gcatgtcgac aagccgaaca
     1261 tatgggcgcg cctagtatgt atgtaagtta ataaaaccca tttttgcgga aagtagataa
     1321 aaaaaacatt tttttttttt actgcactgg atatcattga acttatctga tcagttttaa
     1381 atttacttcg atccaagggt atttgatgta ccaggttctt tcgattacct ctcactcaaa
     1441 atgacattcc actcaaagtc agcgctgttt gcctccttct ctgtccacag aaatatcgcc
     1501 gtctctttcg ccgctgcgtc cgctatctct ttcgccaccg tttgtagcgt tacgtagcgt
     1561 caatgtccgc cttcagttgc attttgtcag cggtttcgtg acgaagctcc aagcggttta
     1621 cgccatcaat taaacacaaa gtgctgtgcc aaaactcctc tcgcttctta tttttgtttg
     1681 ttttttgagt gattggggtg gtgattggtt ttgggtgggt aagcagggga aagtgtgaaa
     1741 aatcccggca atgggccaag aggatcagga gctattaatt cgcggaggca gcaaacaccc
     1801 atctgccgag catctgaaca atgtgagtag tacatgtgca tacatcttaa gttcacttga
     1861 tctataggaa ctgcgattgc aacatcaaat tgtctgcggc gtgagaactg cgacccacaa
     1921 aaatcccaaa ccgcaattgc acaaacaaat agtgacacga aacagattat tctggtagct
     1981 gttctcgcta tataagacaa tttttgagat catatcatga tcaagacatc taaaggcatt
     2041 cattttcgac tatattcttt tttacaaaaa atataacaac cagatatttt aagctgatcc
     2101 tagatgcaca aaaaataaat aaaagtataa acctacttcg taggatactt cggggtactt
     2161 tttgttcggg gttagatgag cataacgctt gtagttgata tttgagatcc cctatcattg
     2221 cagggtgaca gcggagcggc ttcgcagagc tgcattaacc agggcttcgg gcaggccaaa
     2281 aactacggca cgctccggcc acccagtccg ccggaggact ccggttcagg gagcggccaa
     2341 ctagccgaga acctcaccta tgcctggcac aatatggaca tctttggggc ggtcaatcag
     2401 ccgggctccg gatggcggca gctggtcaac cggacacgcg gactattctg caacgagcga
     2461 cacataccgg cgcccaggaa acatttgctc aagaacggtg agtttctatt cgcagtcggc
     2521 tgatctgtgt gaaatcttaa taaagggtcc aattaccaat ttgaaactca gtttgcggcg
     2581 tggcctatcc gggcgaactt ttggccgtga tgggcagttc cggtgccgga aagacgaccc
     2641 tgctgaatgc ccttgccttt cgatcgccgc agggcatcca agtatcgcca tccgggatgc
     2701 gactgctcaa tggccaacct gtggacgcca aggagatgca ggccaggtgc gcctatgtcc
     2761 agcaggatga cctctttatc ggctccctaa cggccaggga acacctgatt ttccaagcca
     2821 tggtgcggat gccacgacat ctgacctatc ggcagcgagt ggcccgcgtg gatcaggtga
     2881 tccaggagct ttcgctcagc aaatgtcagc acacgatcat cggtgtgccc ggcagggtga
     2941 aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc ctccgaggct ctaaccgatc
     3001 cgccgcttct gatctgcgat gagcccacct ccggactgga ctcctttacc gcccacagcg
     3061 tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac cgtcatcctg accattcatc
     3121 agccgtcttc cgagctgttt gagctctttg acaagatcct tctgatggcc gagggcaggg
     3181 tagctttctt gggcactccc agcgaagccg tcgacttctt ttcctagtga gttcgatgtg
     3241 tttattaagg gtatctagta ttacataaca tctcaactcc tatccagcgt gggtgcccag
     3301 tgtcctacca actacaatcc ggcggacttt tacgtacagg tgttggccgt tgtgcccgga
     3361 cgggagatcg agtcccgtga tcggatcgcc aagatatgcg acaattttgc cattagcaaa
     3421 gtagcccggg atatggagca gttgttggcc accaaaaatc tggagaagcc actggagcag
     3481 ccggagaatg ggtacaccta caaggccacc tggttcatgc agttccgggc ggtcctgtgg
     3541 cgatcctggc tgtcggtgct caaggaacca ctcctcgtaa aagtgcgact tattcagaca
     3601 acggtgagtg gttccagtgg aaacaaatga tataacgctt acaattcttg gaaacaaatt
     3661 cgctagattt tagatagaat tgcctgattc cacacccttc ttagtttttt tcaatgagat
     3721 gtatagttta tagttttgca gaagataaat aaatttcatt taactcgcga atattaatga
     3781 gatgcgagta acattttaat ttgcagatgg ttgccatctt gattggcctc atctttttgg
     3841 gccaacaact cacgcaagtg ggtgtgatga atatcaacgg agccatcttc ctcttcctga
     3901 ccaacatgac ctttcaaaac gtctttgcca cgataaatgt aagtcatgtt tagaatacat
     3961 ttgcatttca ataatttact aactttctaa tgaatcgatt cgatttaggt gttcacctca
     4021 gagctgccag tttttatgag ggaggcccga agtcgacttt atcgctgtga cacatacttt
     4081 ctgggcaaaa cgattgccga attgccgctt tttctcacag tgccactggt cttcacggcg
     4141 attgcctatc cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg
     4201 ctggtcactc tggtggccaa tgtgtcaacg tccttcggat atctaatatc ctgcgccagc
     4261 tcctcgacct cgatggcgct gtctgtgggt ccgccggtta tcataccatt cctgctcttt
     4321 ggcggcttct tcttgaactc gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc
     4381 tcatggttcc gttacgccaa cgagggtctg ctgattaacc aatgggcgga cgtggagccg
     4441 ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg
     4501 gagacgctta acttctccgc cgccgatctg ccgctggact acgtgggtct ggccattctc
     4561 atcgtgagct tccgggtgct cgcatatctg gctctaagac ttcgggcccg acgcaaggag
     4621 tagccgacat atatccgaaa taactgcttg tttttttttt ttaccattat taccatcgtg
     4681 tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca ataaaaacaa
     4741 gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt agactttgga
     4801 tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa ctcgtttatc
     4861 gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg cgcgccctct
     4921 tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc tcccacccgc
     4981 tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt tagttccgcg
     5041 cgattcggtt cgctcaaatg gttccgagtg gttcatttcg tctcaataga aattagtaat
     5101 aaatatttgt atgtacaatt tatttgctcc aatatatttg tatatatttc cctcacagct
     5161 atatttattc taatttaata ttatgacttt ttaaggtaat tttttgtgac ctgttcggag
     5221 tgattagcgt tacaatttga actgaaagtg acatccagtg tttgttcctt gtgtagatgc
     5281 atctcaaaaa aatggtgggc ataatagtgt tgtttatata tatcaaaaat aacaactata
     5341 ataataagaa tacatttaat ttagaaaatg cttggatttc actggaacta gggcgcgcct
     5401 ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa
     5461 accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca
     5521 cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag
     5581 ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccagggcc gcacatatgc
     5641 aatggttaat tcgagctcgc ccggggctct caagtgtatc tgagactcaa gtgtgctcac
     5701 ttgagtgctc tcaagtgtat gaccacactt gagtgctctc aagtgttcga tctctcaagt
     5761 gtacttcact caagtgtatg atgctctcaa gtgtatccaa gcttatcgat ttcgaaccct
     5821 cgaccgccgg agtataaata gaggcgcttc gtctacggag cgacaattca attcaaacaa
     5881 gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc
     5941 taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa
     6001 gtaaatcaac tgcaactact gaaatctgcc aagaagtaat tattgaatac aagaagagaa
     6061 ctctgaatag atctaaaagg taggttcaac cactgatgcc taggcacacc gaaacgacta
     6121 accctaattc ttatccttta cttcaggcgg ccgcggctcg agggtaccaa tcaaacatgt
     6181 ccaaaggtga agaactgttt accggagtag tcccgatatt ggttgaactc gacggcgatg
     6241 tcaacggtca taaattcagt gtgtccggcg agggtgaggg cgacgccaca tacggtaagc
     6301 tgacgttgaa gttcatatgc accacgggca agctgcccgt gccatggccg acgttggtca
     6361 cgacgctgac gtatggtgtc cagtgtttca gccgttaccc cgatcatatg aagcagcatg
     6421 actttttcaa gtcggcgatg ccggagggat acgttcaaga gaggaccatt ttcttcaagg
     6481 atgacggcaa ctataagacg cgagcggagg tgaaatttga aggcgacaca ctcgttaacc
     6541 gtattgagtt gaagggcatt gattttaagg aggatggcaa cattctgggc cataagttgg
     6601 aatacaacta caactcgcat aatgtgtata taatggcaga taagcagaaa aacggaataa
     6661 aggttaactt caagattcgc cacaacatag aggacggttc cgtgcaactt gcagatcatt
     6721 accaacagaa cacacccatt ggagatggcc cagttctctt gccagacaat cactaccttt
     6781 ccacacagtc cgcgttgagc aaggacccca atgaaaagcg ggaccacatg gtgttgctgg
     6841 agtttgtgac cgcagctggt attacacacg gcatggatga gctctacaag taatctagag
     6901 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
     6961 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
     7021 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
     7081 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
     7141 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
     7201 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
     7261 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
     7321 tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     7381 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     7441 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     7501 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7561 tgtccaaact catcaatgta tcttatcatg tctggatcga tctggccggc gatatctata
     7621 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
     7681 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
     7741 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
     7801 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     7861 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggg
     7921 ccggccgttt aaacgaattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     7981 gttaatgtca tgataataat ggtttcttag actcaggtgg cacttttcgg ggaaatgtgc
     8041 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8101 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8161 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8221 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8281 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8341 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8401 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8461 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8521 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8581 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8641 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8701 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8761 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8821 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8881 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8941 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9001 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9061 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9121 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9181 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9241 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9301 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9361 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9421 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9481 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9541 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9601 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9661 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9721 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9781 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttaccg ccctagaaag
     9841 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
     9901 tc
//
