LOCUS       sequence-357815-        2641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR plasmid carrying yeast recombination site for Gateway cloning 
            into introns of genes encoding fluorescent proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: pENTR{KDRT}
FEATURES             Location/Qualifiers
     source          1..2641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      889..1477
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1378..1397
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1631..1648
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1807..1834)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1926..2012)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2061..2083
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2075..2092
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2076..2092
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2108..2207
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2305..2404)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2421..2440)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2422..2440)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2445..2461)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2445..2461)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2563..2582)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(2574..2641,1..742)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(join(2641,1..19))
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
       61 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
      121 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
      181 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
      241 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
      301 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
      361 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
      421 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
      481 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
      541 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
      601 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
      661 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
      721 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
      781 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
      841 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      901 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      961 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1021 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1081 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1141 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1201 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1261 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1321 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1381 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1441 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1501 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1561 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     1621 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     1681 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1741 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
     1801 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
     1861 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
     1921 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     1981 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
     2041 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
     2101 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
     2161 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctccg cggccgcccc
     2221 cttcaccaaa cgatatcaga catttgtctg ataatgcttc attatcagac aaatgtctga
     2281 tatcgtttaa gggtgggcgc gccgacccag ctttcttgta caaagttggc attataagaa
     2341 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     2401 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     2461 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2521 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     2581 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     2641 g
//
