LOCUS       sequence-357816-        2614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR plasmid carrying yeast recombination site for Gateway cloning 
            into introns of genes encoding fluorescent proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: pENTR{FRT}
FEATURES             Location/Qualifiers
     source          1..2614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    23..122
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(143..176)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(193..292)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(309..328)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(310..328)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(333..349)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(333..349)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(451..470)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             462..1271
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(529..548)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1418..2006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1907..1926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2160..2177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2336..2363)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2455..2541)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2590..2612
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(2604..2614,1..7)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(2605..2614,1..7)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc
       61 gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagcagg
      121 ctccgcggcc gcccccttca ccgaagttcc tatactttct agagaatagg aacttcaagg
      181 gtgggcgcgc cgacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat
      241 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
      301 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
      361 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
      421 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
      481 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
      541 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
      601 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
      661 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
      721 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
      781 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
      841 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
      901 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
      961 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     1021 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     1081 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     1141 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     1201 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     1261 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     1321 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     1381 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1441 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1501 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1561 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1621 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1681 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1741 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1801 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1861 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1921 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1981 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2041 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2101 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2161 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     2221 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2281 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     2341 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     2401 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     2461 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     2521 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     2581 ggatgttttc ccagtcacga cgttgtaaaa cgac
//
