LOCUS       sequence-357822-        2614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR plasmid carrying yeast recombination site for Gateway cloning 
            into introns of genes encoding fluorescent proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: pENTR{FRT-RC}
FEATURES             Location/Qualifiers
     source          1..2614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    62..95
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(112..211)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(228..247)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(229..247)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(252..268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(252..268)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(370..389)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             381..1190
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(448..467)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1337..1925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1826..1845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2079..2096
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2255..2282)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2374..2460)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2509..2531
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2523..2540
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2524..2540
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(2556..2614,1..41)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc tccgcggccg cccccttcac
       61 cgaagttcct attctctaga aagtatagga acttcaaggg tgggcgcgcc gacccagctt
      121 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
      181 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
      241 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
      301 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
      361 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
      421 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
      481 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
      541 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
      601 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
      661 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
      721 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
      781 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
      841 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
      901 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
      961 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     1021 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     1081 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     1141 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     1201 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     1261 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     1321 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1381 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1441 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1501 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1561 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1621 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1681 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1741 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1801 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1861 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1921 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1981 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2041 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2101 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2161 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     2221 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     2281 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     2341 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     2401 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     2461 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
     2521 gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta ttttgactga
     2581 tagtgacctg ttcgttgcaa caaattgatg agca
//
