LOCUS       sequence-357823-        8703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHelz_1213-2103.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 8703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmHelz_1213-2103_AH
FEATURES             Location/Qualifiers
     source          1..8703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2575..2596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2836..2855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3183..3204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5954..5995
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6005..6022
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6045..6062)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6095..6229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6119..6138)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6173..6192
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6830..7418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6910..6929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7589..8380,8381..8449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8212..8231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8450..8554)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8622..8640
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2881 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2941 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3001 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3121 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3241 tacaagccaa gcttccccgt ggctctttgt tccattggtc gctgccggaa agtgtgggag
     3301 cgtttcatcg aaatctgcga cgaacataaa agtctttttg gcttaacttg gtcgaacctg
     3361 cgctcccaac tggatggcgt ggagcttaag cgtggctatg tgctaaatcc tctggctccc
     3421 gagttcgtcc cgcgtgccct tcaaccggag gcatatctgc gggagcaggc tgctctctat
     3481 ctaaatggtc cgcaacacgg acacggcggc catggaccgc cgggtcctcc ggctcatttt
     3541 gccaacgcag ctggaatgct gggtcccgga cccgcggccg ctgctcacca gatgaatcaa
     3601 atggctgctg ccatggctgc caatcagcaa cagctcacag cccacatgta ctccacccac
     3661 atggccatga tggccgctgc cgtcggagga aagtctggaa atggccctgg accaggaacc
     3721 ggcggaggac ccggtcctag tccaggccca cctccacact acggcggcgg cggtggtggt
     3781 ggcggacaca tgggaatgag aggaccaccg ccaccggcgc cgcatctgcg tggaatgcca
     3841 cccggtggac cgccacccac acagcagcca ggcggagctg gtggcggcgg gggtccaccg
     3901 ccaccatacg gacaatatcc agccatgcag cactggcgac caccgcagca gggacagaat
     3961 caaggcccag gacagcagcc accacaaaat ccgaatgcca gtctctgggg tccgccgccg
     4021 caaacgaatc cgtggagtgt gctgccgccc agtaagcagc cacagcaacc tcagccaccg
     4081 ccagttagcg cgcccggacg tggaccgggt cctttgccac cgccactcaa tgcaaacccg
     4141 tctcttccac tgagaggtgg tagtgtgccg ccaccgccgc cgaatgcctc gactgctgct
     4201 cacctgctaa ggaatcccag cgaactgggc tacttggcca agaagtcgct gtacaaccat
     4261 ggccaggcat tgccgcatca tcagctttat ggacccggac caggaccacc gccgcctcca
     4321 tcgcagcaac agccgcaggc accgccgatg ggtatgcaga ggggcagcag tgtgccgaac
     4381 ggacccatgt cgcccatgga gaacggaccg ccgccacctc cctatctgag gcacaatggt
     4441 agtggctaca atccgggagc accgccaccg cctccgcagc aacaaccaca gccaccaccc
     4501 aatccgttta tgtacgccgg caagcaaccg tctccgagct ctatgagatt tggaccaatg
     4561 acgcacgagc tactcccacc caatgtaagc atctatgaca tggcttttaa tccgaaagac
     4621 gagcaataca agtggtacgt taaactgctt gaaaccgtgg gtcaggatgg tgccaacaag
     4681 tttgcggaaa tgctgcgcca ggtgatggcc acaatgcaac agcagcagca gcaacataag
     4741 ccaccgcagc caccgatggc taacaatccg atgctcaata atccgcacgt gcagcgacca
     4801 cagtatccca tgatgtatcc acagggtcaa cagcaacagc agcagcagcc cccacagcaa
     4861 tctgtagatg cttcaccgcc gctggagaac ttcaaccagc agatcgatct tgttttcaat
     4921 tcgattatga atggagccaa tgatgttgcg cagcccatac tgcgcgatgt cctgggcatg
     4981 gtagctggca cgggcaatgc tcctggtcct ccgcttagca acggtttgaa cggagtcgat
     5041 ctccagcagc agcagcagca acaacagcag cagcagcaaa gccgcctatt cgcaatgatg
     5101 cagcaacaac aacagcagca gcaacaacaa caggccaagc caccgctagg acctggacca
     5161 ctttatccca atcatctggg cggacctggt cttcaccagg cgccgcccaa tggtggaggt
     5221 aatctgagcg gcgtcggaaa caccaacttt atgggcaacg ccggaaatgc tctgctcaat
     5281 gacaagccct acaacaacgt gggcatgcac aacaacgtgg gcttgaacat cggtggcgca
     5341 ggagttgctg gtcacaatag taataacaac aatggaatgc tgaccaatgg caacaactcg
     5401 gtgcccttgt atcgccgcca ggcgcacacc ggaaacggga ttggaggcgg aaatggtgac
     5461 tacaacaaca tgcatggaat ggatgctggc gccaacttga tagaggcact cttccaggca
     5521 aacaactcgg tggtggatca ctcggtgaag tcgtcggacc atatgcacgg cctgctgtac
     5581 aacaatttca atgggctgaa gggcggcggc cacgaactgg acctgttcca ggcgatggcg
     5641 cccagtgtgc cgcactcgga ggcagctaat catcaccagc agtcctctgg ctcatcggca
     5701 accacctatg cggctgtgct gagccaggga ccacaggcgg tggttggcgg aggcgttgga
     5761 acaggaccat cgccgggaca gtcgcaggcg caggcgggcg tggctggttc aggaacgggc
     5821 ggaaaagtcg cgccggcggt tggtggtaac gatcttctgg agaaggatcc gtttgcggcc
     5881 atccgggagt tgggacaggc cacgaccgga ttctacaact attttcagta atctagaggg
     5941 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     6001 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
     6061 taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa gatacattga
     6121 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     6181 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     6241 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     6301 aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc ttgagagcct
     6361 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     6421 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     6481 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     6541 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     6601 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     6661 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     6721 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     6781 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6841 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6901 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6961 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7021 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7081 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7141 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7201 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7261 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7321 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7381 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7441 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7501 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7561 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7621 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7681 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7741 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7801 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7861 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     7921 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7981 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8041 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8101 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8161 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     8221 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8281 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8341 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8401 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8461 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8521 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8581 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     8641 cctttcgtct tcaagaatta attctatatt ctaaaaacac aaatgatact tctaaaaaaa
     8701 aat
//
