LOCUS       sequence-357825-        5544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector of PIK3CA E545K for gateway cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Wang C, Che L, Hu J, Zhang S, Jiang L, Latte G, Demartis MI, Tao J, 
            Gui B, Pilo MG, Ribback S, Dombrowski F, Evert M, Calvisi DF, Chen X
  TITLE     Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to 
            induce liver tumor formation in mice via AKT2/mTORC1 cascade.
  JOURNAL   Liver Int. 2015 Dec 30. doi: 10.1111/liv.13055.
  PUBMED    26716908
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Liver Int. 
            2015 Dec 30. doi: 10.1111/liv.13055."
COMMENT     Sequence Label: pENTR1A-PIK3CA E545K
FEATURES             Location/Qualifiers
     source          1..5544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(45..131)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(296..884)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(376..395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(974..1783)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1697..1716
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1775..1794
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1906..2005
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5248..5274)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5321..5420)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5439..5462)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(5470..5497)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
       61 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
      121 cgttttattt gatgcctggc agttccctac tctcgcttag tagttagacg tccccgagat
      181 ccatgctagc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      241 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      301 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      421 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      481 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      541 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      601 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      661 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      721 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      781 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      841 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      901 tttctacggg gtctgacgct cagtggaacg gggcccaatc tgaataatgt tacaaccaat
      961 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1021 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1081 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1141 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1201 catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt
     1261 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1321 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1381 aaacaggaat cgaatgcaac cggcgcaggg acactgccag cgcatcaaca atattttcac
     1441 ctgaatcagg atattcttct aatacctgga atgctgtttt tccggggatc gcagtggtga
     1501 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1561 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1621 catgtttcag aaacaactct ggcgcatcgg gcttcccata caagcgatag attgtcgcac
     1681 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1741 aatttaatcg cggcctcgac gtttcccgtt gaatatggct cataacaccc cttgtattac
     1801 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     1861 aacatcagag attttgagac acgggccaga gctgcagctg gatggcaaat aatgatttta
     1921 ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt tcttataatg
     1981 ccaactttgt acaagaaagc tgggtctaga tatctcgact cagttcaatg catgctgttt
     2041 aattgtgtgg aagatccaat ccatttttgt tgtccagcca ccatgatgtg catcattcat
     2101 ttgtttcatg aaatactcca aagcctcttg ctcagtttta tctaaggcta gggtctttcg
     2161 aatgtatgca atgtcatcaa aagattgtag ttctggcatt ccagagccaa gcatcattga
     2221 gaaaagattt atgaagagat tggcatgctg tcgaatagct agataagcct tgtaacacat
     2281 ctcctgaaac ctctcaaatt ctcttgtctt tgtgcattct tgggctcctt tactaatcac
     2341 tattaagaaa tcctgtgtca aaacaaatgg cacacgttct cgtttataac caaatttttt
     2401 cttcttgtga tccaaaaagt gtccaaaatc tatatgaaac agttgtccat cgtctttcac
     2461 catgatgtta ctattgtgac gatctccaat tcccaaaatg aaggtagcta cacagtatcc
     2521 agcacatgaa cgtgtaaaca ggtcaatggc tgcatcatat atttctcctt tgttcttgtc
     2581 tttgagccac tgatgtagtg tgtggctgtt gaactgcagt gcacctttca agccgccttt
     2641 gcactgaatt tgcataatag tgtgagaatt tcgcaccacc tcaataagtc ccacacagtc
     2701 accgattgac agacaaccat aaggtaacat tcgaagatca agaccttgat tttgccagat
     2761 attttccata atacgaataa tttgaagtgt tagcatatct tgccgtaaat catccccatt
     2821 tttaaagatg atctcattgt tctgaaacag taactctgac atgatgtctg ggttctccca
     2881 attcaaccac agtggccttt ttgcagagga cataattcga cactcttcaa gcctgaggtt
     2941 tcctagttga tgagcagggt ttagaggaga cagaaagccc tgtagagcat ccatgaaatc
     3001 tggtcgcctc atttgctcaa ctaaaaactt catctgtacc ttttgtgttt catccttctt
     3061 ctcctgtttg agaatgtcag ttaagttaat gagcttttcc attgcctcga cttgcctatt
     3121 caggtgcttc aaatacatcc cacatgcacg acaataggac tccaaaagca ggccaaacct
     3181 ctggctaact gttttattgt gcatctcaga ttttaaatgc caaaagaaaa agtgcccaat
     3241 cctttgatta gtcaatgctt tcttcagtaa aaatctcaca agcaagttat ccaaatattg
     3301 ttcatatttt aggacctgta ctagctgaat taaatactga gaaagtttgt catctgttaa
     3361 atatttttcc aagcaccgaa cagcaaaacc tcgaaccata ggatctgggt aattacagtc
     3421 cagaagttcc atagcctgtt caggtttgat tggaggccaa tcttttacca agcaatacat
     3481 ctgggctact tcatctctag aattccattt aacagacaga agcaatttgg gtagaatttc
     3541 ggggatagtt acacaatagt gtctgtgact ccatagaaaa tctttctcct gcttagtgat
     3601 ttcagagaga ggatctcgtg tagaaattgc tttgagctgt tctttgtcat tttcccttaa
     3661 ttcattgtct ctagctagtc tgttactcag tcctgcgtgg gaatagctaa atcctgcttc
     3721 tcgggataca gaccaattgg catgctcttc aatcactgac atatctggga actttaccac
     3781 actgctgaac cagtcaaact ccaactctaa gcatggagtt tctttatttg gatttgatcc
     3841 agtaacacca atagggttca gcaaatcttc taatccatga ggtactggcc aaagattcaa
     3901 agccattttt ccagatacta gagtgtctgt gtaatcaaac aagtttatat ttccccatgc
     3961 caatggacag tgttcctctt tagcaccctt tcggccttta acagagcaaa tggaaaggca
     4021 aagtcgagca gcacgaggaa gatcaggaat gtatatatca taattcagcc attcattcca
     4081 cctgggattg gaacaaggta ctctttgagt gttcacattg tcacataagg gttctcctcc
     4141 atggtagata cctgttcgaa catagatctt atcaatgtct cgaatattta cattcacgta
     4201 ggttgcacaa agaattttta ttctgagtgc actatttata acccaaaggg attttgtaga
     4261 tgtttctcca ttcatatatg gtgtagctgt ggaaatgcgt ctggaataag atggcattgt
     4321 aaaacagtcc attggcagtt gagaataaag gctttcttta gccatcaaca tcaaattggg
     4381 catcctccca agcattatac agcttcttat atacttatac tgactcagag gatatttttc
     4441 taggaagtat tcatcacatc cacacacttt taaaatatac ttgccctgat attctaaaac
     4501 acagagtttt agttgttcag aggatagcaa catacttcga gtttttttcc tgattgcttc
     4561 agcaattact tgttctggta cacagtcatg gttgattttc agagtatact tctgcttgtc
     4621 attatttgga gaaactatta cccagatcac cactattatt tgccctttat ctaatttatt
     4681 atatatgtgc tttggcaatt ctggtgaaga ttctacattt ggaggataga catacattgc
     4741 tctactatga ggtgaattga ggtccctaag atccacagct tctttacaaa cgttcagaac
     4801 atttcttcgg aagtcctgta cttctggatc tttaaccata tcaaattcac acactggcat
     4861 gccgatagca aaaccaattt ctcgattgag gatcttttct tcacggttgc ctactggttc
     4921 aattactttt aaaaagggtt gaaaaagccg aaggtcacaa agtcgtcttg tttcatcaaa
     4981 aaattcttcc ctttctgctt cttgagtaac acttacgaaa atgtaagaag attcatcttg
     5041 aagaagttga tggagggggt attttcttgc ttctttaaat agttcatgct ttatggttat
     5101 taatgtagcc tcacggaggc attctaaagt cactatcatt ccatttggta gtaaacattc
     5161 tactaggatt cttgggggca tcaagtggat gccccacagt tcacctgatg atggtcttgg
     5221 agggtacgta ccaccacact gggatctggc gtaatctggc acgtcgtatg ggtaccccat
     5281 ggtgaagctt ggatccagtc gactgaattg gttcctttaa agcctgcttt tttgtacaaa
     5341 gttggcatta taaaaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     5401 caaaataaaa tcattatttg gggcccgagc ttaagtaact aactaacagg aagagtttgt
     5461 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt
     5521 tatggcgggc gtcctgcccg ccac
//
