LOCUS       sequence-357861-        2685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CMV-driven expression of an N-terminally Flag-tagged MIOS (core 
            subunit of GATOR2) — native sequence for transient expression in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Valenstein ML, Rogala KB, Lalgudi PV, Brignole EJ, Gu X, Saxton RA, 
            Chantranupong L, Kolibius J, Quast JP, Sabatini DM
  TITLE     Structure of the nutrient-sensing hub GATOR2.
  JOURNAL   Nature. 2022 Jul;607(7919):610-616. doi: 10.1038/s41586-022-04939-z.
            Epub 2022 Jul 13.
  PUBMED    35831510
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04939-z"; journalName: "Nature"; date: 
            "2022-07"; volume: "607"; issue: "7919"; pages: "610-616"
COMMENT     Sequence Label: Flag-MIOS(1-875) in pRK5
FEATURES             Location/Qualifiers
     source          1..2685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..33
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggaaatgg actacaagga tgacgatgac aagggtggtg gtggtggtgc gtcgacgatg
       61 tcgggcacca aaccggatat tctgtgggca ccgcatcatg ttgaccgctt cgttgtctgt
      121 gactcggaac tgtccctgta ccatgtcgaa agtaccgtga acagcgaact gaaagcaggc
      181 agcctgcgtc tgtcagaaga ctcggcggcc accctgctga gcattaactc tgatacgccg
      241 tatatgaaat gtgtcgcgtg gtatctgaac tacgatccgg aatgcctgct ggccgttggc
      301 caggcaaatg gtcgcgtggt tctgaccagc ctgggccaag accacaactc aaaattcaaa
      361 gatctgatcg gtaaagaatt tgtgccgaaa catgcccgtc agtgtaacac cctggcatgg
      421 aatccgctgg attctaactg gctggcagct ggcctggaca aacaccgcgc ggatttttct
      481 gtgctgattt gggacatctg cagtaaatat accccggata ttgttccgat ggaaaaagtc
      541 aaactgtccg ctggcgaaac cgaaaccacg ctgctggtta cgaaaccgct gtacgaactg
      601 ggtcagaatg acgcctgtct gtcactgtgc tggctgccgc gtgatcaaaa actgctgctg
      661 gctggtatgc accgtaatct ggcgattttt gatctgcgca acacctcgca gaaaatgttc
      721 gtgaatacca aagcggttca aggcgtgacg gttgacccgt attttcatga tcgtgtcgct
      781 agcttctacg agggtcaggt ggcgatctgg gatctgcgca aatttgaaaa accagtgctg
      841 accctgacgg aacaaccgaa accgctgacc aaagtcgcat ggtgtccgac ccgtacgggc
      901 ctgctggcaa ccctgacgcg tgactctaat attatccgcc tgtatgatat gcagcacacc
      961 ccgacgccga ttggtgacga aaccgaaccg acgattatcg aacgcagtgt gcagccgtgc
     1021 gataactaca ttgcttcgtt cgcgtggcat ccgaccagcc aaaaccgtat gatcgtcgtg
     1081 accccgaatc gcacgatgag cgattttacc gttttcgaac gtatttcact ggcttggtcg
     1141 ccgatcacga gcctgatgtg ggcgtgtggt cgccacctgt atgaatgcac cgaagaagaa
     1201 aacgacaatt cgctggaaaa agatattgcc acgaaaatgc gtctgcgcgc actgagccgt
     1261 tacggcctgg acaccgaaca agtgtggcgc aatcacatcc tggccggtaa cgaagatccg
     1321 caactgaaat ccctgtggta taccctgcat tttatgaaac agtacacgga agatatggac
     1381 caaaaatcgc cgggcaataa aggtagcctg gtttatgcgg gcattaaaag catcgtgaaa
     1441 agctctctgg gtatggttga aagttcccgt cataactggt ctggcctgga taaacagagt
     1501 gacattcaaa acctgaatga agaacgcatc ctggccctgc agctgtgtgg ctggattaag
     1561 aaaggcaccg atgtggatgt gggcccgttt ctgaacagcc tggtgcaaga aggtgaatgg
     1621 gaacgcgcgg cggcagttgc gctgttcaac ctggatattc gtcgcgccat tcagatcctg
     1681 aatgaaggcg catcatcgga aaaaggtgat ctgaacctga atgttgtcgc tatggcgctg
     1741 agtggttata ccgatgagaa aaacagcctg tggcgtgaaa tgtgtagtac gctgcgcctg
     1801 cagctgaaca atccgtacct gtgcgtgatg tttgctttcc tgacctctga aacgggcagt
     1861 tatgatggtg tcctgtacga aaataaagtt gcggtccgtg accgcgtggc ctttgcatgt
     1921 aaattcctgt cagataccca gctgaaccgt tatatcgaaa aactgacgaa cgaaatgaaa
     1981 gaagccggca acctggaagg tatcctgctg accggcctga cgaaagatgg tgttgacctg
     2041 atggaaagct atgtcgatcg caccggcgac gtgcagacgg catcctactg catgctgcaa
     2101 ggttcaccgc tggatgttct gaaagacgaa cgtgtccagt attggattga aaactaccgc
     2161 aatctgctgg atgcttggcg tttttggcac aaacgcgcgg aatttgacat ccatcgttct
     2221 aaactggatc cgagctctaa accgctggcc caggtgtttg ttagttgtaa cttctgcggc
     2281 aaatcaattt cgtatagctg ctctgcagtg ccgcatcagg gtcgcggttt tagccaatac
     2341 ggcgttagtg gttccccgac caaatccaaa gtcacgtcat gtccgggttg ccgtaaaccg
     2401 ctgccgcgtt gtgcactgtg cctgatcaat atgggtaccc cggtgtcttc ctgtccgggc
     2461 ggtacgaaat cggatgaaaa agttgacctg agcaaagata aaaaactggc ccagtttaac
     2521 aattggttca cctggtgtca caactgccgt catggcggtc acgcaggtca tatgctgtcc
     2581 tggtttcgcg atcatgctga atgcccggtg tcagcgtgca cctgtaaatg catgcaactg
     2641 gatacgacgg gtaatctggt cccggcggaa accgttcaac cgtaa
//
