LOCUS       sequence-357867-        9341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Liu H, Wang HG, Pitt GS, Liu ZJ
  TITLE     Direct Observation of Compartment-Specific Localization and Dynamics
            of Voltage-Gated Sodium Channels.
  JOURNAL   J Neurosci. 2022 Jun 6;42(28):5482-98. doi: 
            10.1523/JNEUROSCI.0086-22.2022.
  PUBMED    35672149
REFERENCE   2  (bases 1 to 9341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0086-22"; journalName: "J Neurosci"; date: 
            "2022-06-6- 6"; volume: "42"; issue: "28"; pages: "5482-98"
COMMENT     Sequence Label: piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..9341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1552..2262
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1552..2262
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1579..1607)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2202..2222
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2272..2328
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2329..3039
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2474..2492)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2742..2761
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3071..3643
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3238..3255)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3465..3484
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3652..4446
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3706..3725)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4316..4335
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4506..4526)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    4568..4689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4605..4624)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4659..4678
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5452..5486
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5977..5993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5977..5993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5990..6012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6001..6017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6025..6031,6032..6049,6050..6055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6070..6091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6208..6225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6379..6967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6459..6478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7138..7929,7930..7998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7761..7780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7999..8103)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8129..8584)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8266..8287)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8478..8497
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8711..8733
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8725..8742
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8726..8742
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8872..8934
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ggcctccacg gccaattcca tatgtgacga ccgaccccga cccactggac aagcacccaa
       61 cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac aggatgcggc
      121 gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc ccgcctggcg
      181 gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc
      241 aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca gccggaccgc
      301 accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg
      361 actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc
      421 agctgtgctc ctgggcaccg cgcagtccgc ccccgcggct cctggccaga ccacccctag
      481 gaccccctgc cccaagtcgc agccaccggt atggtggtgg ccatggctta cgaagagcag
      541 aaccaggcaa cactggagga ggcagagcag aaagaggcag agttcaaggc gatgctcgag
      601 cagctcaaga agcagcagga ggaggcgcag gctgctgcaa tggccacttc ggcgggcacc
      661 gtctcagaag atgccattga agaagaaggg gaagatgggg taggctctcc gaggagctca
      721 tctgagctgt ctaaactcag ttccaagagc gcgaaggagc gtcggaaccg acggaagaag
      781 aggaagcaga aggagctctc tgaaggggag gagaaggggg accccgagaa ggtgtttaag
      841 tcagagtcgg aagatggcat gagaaggaag gccttccggc tgccagacaa taggataggg
      901 aggaaatttt ccatcatgaa tcagtcgctg ctcagcatcc caggctcgcc cttcctttcc
      961 cgacacaaca gcaaaagcag catcttcagc tttcggggtc ctggacggtt ccgggacccc
     1021 ggttcggaga acgagttcgc agacgacgag cacagcacag tggaggaaag tgagggccgg
     1081 cgcgactcgc tcttcatccc gatccgcgcc cgcgagcgcc gcagcagcta ctctggatac
     1141 tcaggataca gtcagtgcag ccgctcgtcg cgcatcttcc ccagcctgcg gcgcagcgtg
     1201 aaacgcaaca gcaccgtgga ctgcaatggc gtggtgtcgc tcatcgggcc tggctcgcac
     1261 atcgggcggc tcctgcctga ggcaacaact gaggtggaga ttaagaagaa aggccctgga
     1321 tcgctcttag tctccatgga acaactcgcc tcctacggac ggaaggacag aatcaacagt
     1381 ataatgagcg ttgtcacaaa cacactggtg gaagagctgg aagagtctca gagaaagtgc
     1441 ccaccatgct ggtataagtt tgccaatact ttcctcatct gggagtgtca cccctactgg
     1501 ataaaactga aggagattgt gaacctgatc gtcatggacc ctggaagcgg tagcaaagga
     1561 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     1621 cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa actcaccctt
     1681 aaatttattt gcactactgg aaaactacct gttccgtggc caacacttgt cactactctg
     1741 acctatggtg ttcaatgctt ttcccgttat ccggatcaca tgaaacggca tgactttttc
     1801 aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg
     1861 acctacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag
     1921 ttaaagggta ttgattttaa agaagatgga aacattcttg gacacaaact cgagtacaac
     1981 tttaactcac acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac
     2041 ttcaaaattc gccacaacgt tgaagatggt tccgttcaac tagcagacca ttatcaacaa
     2101 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa
     2161 tctgtccttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta
     2221 actgctgctg ggattacaca tggcatggat gagctctaca aaggatccgg cgcaacaaac
     2281 ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat ggtgagcaag
     2341 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     2401 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2461 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     2521 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     2581 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     2641 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     2701 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     2761 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     2821 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     2881 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     2941 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     3001 cgccactcca ccggcggcat ggacgagctg tacaagtaag aattcggctt ctcgagggat
     3061 tgcggccgcg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     3121 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     3181 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     3241 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     3301 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     3361 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     3421 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     3481 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     3541 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     3601 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata atacctccgg aatgattgaa
     3661 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3721 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3781 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     3841 gcagcgcggc tatcgtggct ggccgcgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3901 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     3961 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4021 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4081 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     4141 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     4201 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     4261 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     4321 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     4381 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     4441 ttctgagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4501 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgcg
     4561 ttaactaaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4621 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4681 tgtatcttat catgtctgga attgactcaa atgatgtcaa ttagtctatc agaagctcat
     4741 ctggtctccc ttccggggga caagacatcc ctgtttaata tttaaacagc agtgttccca
     4801 aactgggttc ttatatccct tgctctggtc aaccaggttg cagggtttcc tgtcctcaca
     4861 ggaacgaagt ccctaaagaa acagtggcag ccaggtttag ccccggaatt gactggattc
     4921 cttttttagg gcccattggt atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc
     4981 aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg
     5041 ctgtccccgc acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc
     5101 cgggcggctc gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt
     5161 gggggggggc tgtccctgat atctataaca agaaaatata tatataataa gttatcacgt
     5221 aagtagaaca tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa
     5281 aagataatca tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt
     5341 accgcattga caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc
     5401 acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca
     5461 attttacgca gactatcttt ctagggttaa tctagctgca tcaggatcat atcgtcgggt
     5521 cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag
     5581 cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct
     5641 gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca
     5701 tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc
     5761 ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg
     5821 gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat
     5881 attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc
     5941 gtgagttacc cggcgggcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6001 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6061 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6121 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6181 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6781 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7801 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7861 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7921 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7981 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8101 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     8161 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     8221 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     8281 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     8341 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     8401 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     8461 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     8521 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     8581 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     8641 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     8701 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt
     8761 cacgtttttg aacccgtgga ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg
     8821 atggagcaga tgaagatgct cgacacgctg cagaacacgc agctagatta accctagaaa
     8881 gataatcata ttgtgacgta cgttaaagat aatcatgtgt aaaattgacg catgtgtttt
     8941 atcggtctgt atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt
     9001 tacacttaca tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta
     9061 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttatg agggacagcc
     9121 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     9181 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     9241 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     9301 gctctttgag cctgcagaca cctgggggga tacggggaaa a
//
