LOCUS       sequence-357869-        5560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter gene with yeast recombination site within 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: p{3P3-dsRed::FRT,attB}
FEATURES             Location/Qualifiers
     source          1..5560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1501..1534
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1575..1644
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(1863..1883)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1863..1881)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1902..1918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1902..1918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1915..1937)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1926..1942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1950..1956,1957..1974,1975..1980))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1995..2016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2133..2150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2304..2892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2384..2403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3063..3854,3855..3923))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3686..3705
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3924..4028)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4055..4510
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4142..4161)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4352..4373
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4636..4658
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4650..4667
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4651..4667
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4677..4696
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4677..4695
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4728..4744
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4728..4744
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    5248..5272
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    5289..5322
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(5343..5367)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgcacaaaaa ataaataaaa gtataaacct acttcgtagg atacttcggg gtactttttg
       61 ttcggggtta gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg
      121 taaccaaggg gggacctttg ccatttgctt gggatatttt gtcaccacaa tttcagtatg
      181 gaagcaaggt atatgtcaag caccctgccg acataccaga ctataaaaag ctgtcatttc
      241 ctgaaggatt taaatgggaa agggtcatga actttgaaga cggtggcgtc gttactgtaa
      301 cccaggattc cagtttgcag gatggctgtt tcatctacaa ggtcaagttc attggcgtga
      361 actttccttc cgatggacct gttatgcaaa agaagacaat gggctgggaa gccagcactg
      421 agcgtttgta tcctcgtgat ggcgtgttga aaggagagat tcataaggct ctgaagctga
      481 aagacggtgg tcattaccta gttgaattca aaagtattta catggcaaag aagcctgtgc
      541 agctaccagg gtactactat gttgactcca aactggatat aacaagccac aacgaagact
      601 atacaatcgt tgagcagtat gaaagaaccg agggacgcca ccatctgttc ctttagcggc
      661 catcgaattc gagctcgccc actaagcgtc gcgccacttc aacgctcgat gggagcgtca
      721 ttggtgggcg gggtaaccgt cgaaatcagt gtttacgctt ccaatcgcaa caaaaaattc
      781 actgcaacac tgaaaagcat acgaaaacga tgaagattgt acgagaaacc ataaagtatt
      841 ttatccacaa agacacgtat agcagaaaag ccaagttaac tcggcgataa gttgtgtaca
      901 caagaataaa atcggccaga ttcagtgttg tcagaaataa gaaaacccca ctatgttttt
      961 ctttgccttt tctttctccc agcgatcatt catttcgtgg tgaaagaacg gggtcattgc
     1021 acggagtttc gactgcggga aagcagagct gccgttcact tcgtctataa ttagcgcttt
     1081 ctattttccc cgattcgggc cgctgctgcg cttttccgcc tgctgtttgt ggcaagtgta
     1141 gcagcaggct gtgcacgcag tgtggcatgc acttggcttt ccaccgttgg tatcgattct
     1201 ctgggacgat gagtcattcc tttcggggcc acagcataat cgttgccagc tcaccgaaat
     1261 ggtgacttca tttcttaact gccgtcaagc atgcgattgt acatacatac atatttatat
     1321 atgtacatat ttatgtgact atggtaggtc gatataatag caatcaacgc aagcaaatgt
     1381 gtcagtcctg cttacaggaa cgattctatt tagtaatttt cgttgtataa agtaattatg
     1441 tatgtatgta agccccataa atctgaaaca attaggcaaa accatgcgaa gctctctagt
     1501 ataacttcgt ataatgtatg ctatacgaag ttctcgaggg ggggcccggt accgtcgacg
     1561 atgtaggtca cggtctcgaa gccgcggtgc gggtgccagg gcgtgccctt gggctccccg
     1621 ggcgcgtact ccacctcacc catctggtcc atcatgatga acgggtcgag gtggcggtag
     1681 ttgatcccgg cgaacgcgcg gcgcgccggg aagccctcgc cctcgaaacc gctgggcgcg
     1741 gtggtcacgg tgagcacggg acgtgcgacg gcgtcggcgg gtgcggatac gcggggcagc
     1801 gtcagcgggt tctcgacggt cacggcgggc atgtcgacac tagtggtacc cagcttttgt
     1861 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     1921 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1981 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2041 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2101 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2161 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2221 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2281 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2341 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2401 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2461 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2521 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2581 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2641 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2701 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2761 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2821 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2881 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2941 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3001 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3061 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3121 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3181 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3241 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3301 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3361 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3421 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3481 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3541 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3601 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3661 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3721 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3781 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3841 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3901 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3961 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4021 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     4081 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4141 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4201 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4261 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4321 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4381 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4441 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4501 cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4561 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     4621 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     4681 acgactcact atagggcgaa ttggagctcc accgcggtgg cggccgctct agaactagtg
     4741 gatcccccgg ggatctaatt caattagaga ctaattcaat tagagctaat tcaattagga
     4801 tccaagctta tcgatttcga accctcgacc gccggagtat aaatagaggc gcttcgtcta
     4861 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
     4921 aataaacaag cgcagctgaa caagctaaac aatcgggcgg ccgcactaga gccggtcgcc
     4981 accatgaggt cttccaagaa tgttatcaag gagttcatga ggtttaaggt tcgcatggaa
     5041 ggaacggtca atgggcacga gtttgaaata gaaggcgaag gagaggggag gccatacgaa
     5101 ggccacaata ccgtaaagct taaggtgagt agtacatgtg catacatctt aagttcactt
     5161 gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac
     5221 aaaaatccca aaccgcggcc gtttatcacc actttgtaca agaaagctgg gtcggcgcgc
     5281 ccacccttga agttcctatt ctctagaaag tataggaact tcggtgaagg gggcggccgc
     5341 ggagcctgct tttttgtaca aacttgtgat aaacgaattc ttgaagacga aagggcacaa
     5401 acaaatagtg acacgaaaca gattattctg gtagctgttc tcgctatata agacaatttt
     5461 tgagatcata tcatgatcaa gacatctaaa ggcattcatt ttcgactata ttctttttta
     5521 caaaaaatat aacaaccaga tattttaagc tgatcctaga
//
