LOCUS       sequence-357870-        5587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter gene with yeast recombination site within 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: p{3P3-dsRed::KD,attB}
FEATURES             Location/Qualifiers
     source          1..5587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    286..319
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    360..429
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(648..668)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(648..666)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(687..703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(687..703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(700..722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    711..727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(735..741,742..759,760..765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    780..801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(918..935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1089..1677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1169..1188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1848..2639,2640..2708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2471..2490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2709..2813)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2840..3295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2927..2946)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3137..3158
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3421..3443
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3435..3452
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3436..3452
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3462..3481
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3462..3480
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3513..3529
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3513..3529
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    4033..4057
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4155..4179)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attcctttcg gggccacagc ataatcgttg ccagctcacc gaaatggtga cttcatttct
       61 taactgccgt caagcatgcg attgtacata catacatatt tatatatgta catatttatg
      121 tgactatggt aggtcgatat aatagcaatc aacgcaagca aatgtgtcag tcctgcttac
      181 aggaacgatt ctatttagta attttcgttg tataaagtaa ttatgtatgt atgtaagccc
      241 cataaatctg aaacaattag gcaaaaccat gcgaagctct ctagtataac ttcgtataat
      301 gtatgctata cgaagttctc gagggggggc ccggtaccgt cgacgatgta ggtcacggtc
      361 tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc
      421 tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac
      481 gcgcggcgcg ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc
      541 acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg
      601 acggtcacgg cgggcatgtc gacactagtg gtacccagct tttgttccct ttagtgaggg
      661 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      721 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      781 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      841 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      901 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      961 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1021 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1081 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1141 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1201 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1261 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1321 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1381 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1441 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1501 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1561 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1621 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1681 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1741 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1801 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1861 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1921 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1981 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2041 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2101 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2161 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2221 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2281 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2341 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2401 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2461 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2521 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2581 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2641 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2701 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2761 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2821 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2881 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2941 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3001 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3061 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3121 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3181 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3241 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt
     3301 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     3361 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     3421 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
     3481 gcgaattgga gctccaccgc ggtggcggcc gctctagaac tagtggatcc cccggggatc
     3541 taattcaatt agagactaat tcaattagag ctaattcaat taggatccaa gcttatcgat
     3601 ttcgaaccct cgaccgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
     3661 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
     3721 ctgaacaagc taaacaatcg ggcggccgca ctagagccgg tcgccaccat gaggtcttcc
     3781 aagaatgtta tcaaggagtt catgaggttt aaggttcgca tggaaggaac ggtcaatggg
     3841 cacgagtttg aaatagaagg cgaaggagag gggaggccat acgaaggcca caataccgta
     3901 aagcttaagg tgagtagtac atgtgcatac atcttaagtt cacttgatct ataggaactg
     3961 cgattgcaac atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg
     4021 cggccgttta tcaccacttt gtacaagaaa gctgggtcgg cgcgcccacc cttaaacgat
     4081 atcagacatt tgtctgataa tgaagcatta tcagacaaat gtctgatatc gtttggtgaa
     4141 gggggcggcc gcggagcctg cttttttgta caaacttgtg ataaacgaat tcttgaagac
     4201 gaaagggcac aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata
     4261 taagacaatt tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta
     4321 tattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa
     4381 aaataaataa aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt
     4441 tagatgagca taacgcttgt agttgatatt tgagatcccc tatcattgca ggtaaccaag
     4501 gggggacctt tgccatttgc ttgggatatt ttgtcaccac aatttcagta tggaagcaag
     4561 gtatatgtca agcaccctgc cgacatacca gactataaaa agctgtcatt tcctgaagga
     4621 tttaaatggg aaagggtcat gaactttgaa gacggtggcg tcgttactgt aacccaggat
     4681 tccagtttgc aggatggctg tttcatctac aaggtcaagt tcattggcgt gaactttcct
     4741 tccgatggac ctgttatgca aaagaagaca atgggctggg aagccagcac tgagcgtttg
     4801 tatcctcgtg atggcgtgtt gaaaggagag attcataagg ctctgaagct gaaagacggt
     4861 ggtcattacc tagttgaatt caaaagtatt tacatggcaa agaagcctgt gcagctacca
     4921 gggtactact atgttgactc caaactggat ataacaagcc acaacgaaga ctatacaatc
     4981 gttgagcagt atgaaagaac cgagggacgc caccatctgt tcctttagcg gccatcgaat
     5041 tcgagctcgc ccactaagcg tcgcgccact tcaacgctcg atgggagcgt cattggtggg
     5101 cggggtaacc gtcgaaatca gtgtttacgc ttccaatcgc aacaaaaaat tcactgcaac
     5161 actgaaaagc atacgaaaac gatgaagatt gtacgagaaa ccataaagta ttttatccac
     5221 aaagacacgt atagcagaaa agccaagtta actcggcgat aagttgtgta cacaagaata
     5281 aaatcggcca gattcagtgt tgtcagaaat aagaaaaccc cactatgttt ttctttgcct
     5341 tttctttctc ccagcgatca ttcatttcgt ggtgaaagaa cggggtcatt gcacggagtt
     5401 tcgactgcgg gaaagcagag ctgccgttca cttcgtctat aattagcgct ttctattttc
     5461 cccgattcgg gccgctgctg cgcttttccg cctgctgttt gtggcaagtg tagcagcagg
     5521 ctgtgcacgc agtgtggcat gcacttggct ttccaccgtt ggtatcgatt ctctgggacg
     5581 atgagtc
//
