LOCUS       sequence-357872-        6155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter gene with yeast recombination site within 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: p{Mhc-F4-678-DsRed::FRT-RC,attB}
FEATURES             Location/Qualifiers
     source          1..6155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    516..540
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(557..590)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(611..635)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2329..2362
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2403..2472
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2691..2711)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2691..2709)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2730..2746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2730..2746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2743..2765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2754..2770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2778..2784,2785..2802,2803..2808))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2823..2844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2961..2978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3132..3720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3212..3231)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3891..4682,4683..4751))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4514..4533
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4752..4856)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4883..5338
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4970..4989)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5180..5201
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5464..5486
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5478..5495
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5479..5495
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5505..5524
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5505..5523
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tatatatgca aagcattctg atatatatat ataagtggta tacatataca tattcgtatc
       61 ggatccgtga tctatattta ggattaaaac cctcgaccgc cggagtataa atagaggcgc
      121 ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa
      181 gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tcgggcggcc gcactagagc
      241 cggtcgccac catgaggtct tccaagaatg ttatcaagga gttcatgagg tttaaggttc
      301 gcatggaagg aacggtcaat gggcacgagt ttgaaataga aggcgaagga gaggggaggc
      361 catacgaagg ccacaatacc gtaaagctta aggtgagtag tacatgtgca tacatcttaa
      421 gttcacttga tctataggaa ctgcgattgc aacatcaaat tgtctgcggc gtgagaactg
      481 cgacccacaa aaatcccaaa ccgcggccgt ttatcaccac tttgtacaag aaagctgggt
      541 cggcgcgccc acccttgaag ttcctatact ttctagagaa taggaacttc ggtgaagggg
      601 gcggccgcgg agcctgcttt tttgtacaaa cttgtgataa acgaattctt gaagacgaaa
      661 gggcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc gctatataag
      721 acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt cgactatatt
      781 cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg cacaaaaaat
      841 aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt cggggttaga
      901 tgagcataac gcttgtagtt gatatttgag atcccctatc attgcaggta accaaggggg
      961 gacctttgcc atttgcttgg gatattttgt caccacaatt tcagtatgga agcaaggtat
     1021 atgtcaagca ccctgccgac ataccagact ataaaaagct gtcatttcct gaaggattta
     1081 aatgggaaag ggtcatgaac tttgaagacg gtggcgtcgt tactgtaacc caggattcca
     1141 gtttgcagga tggctgtttc atctacaagg tcaagttcat tggcgtgaac tttccttccg
     1201 atggacctgt tatgcaaaag aagacaatgg gctgggaagc cagcactgag cgtttgtatc
     1261 ctcgtgatgg cgtgttgaaa ggagagattc ataaggctct gaagctgaaa gacggtggtc
     1321 attacctagt tgaattcaaa agtatttaca tggcaaagaa gcctgtgcag ctaccagggt
     1381 actactatgt tgactccaaa ctggatataa caagccacaa cgaagactat acaatcgttg
     1441 agcagtatga aagaaccgag ggacgccacc atctgttcct ttagcggcca tcgaattcga
     1501 gctcgcccac taagcgtcgc gccacttcaa cgctcgatgg gagcgtcatt ggtgggcggg
     1561 gtaaccgtcg aaatcagtgt ttacgcttcc aatcgcaaca aaaaattcac tgcaacactg
     1621 aaaagcatac gaaaacgatg aagattgtac gagaaaccat aaagtatttt atccacaaag
     1681 acacgtatag cagaaaagcc aagttaactc ggcgataagt tgtgtacaca agaataaaat
     1741 cggccagatt cagtgttgtc agaaataaga aaaccccact atgtttttct ttgccttttc
     1801 tttctcccag cgatcattca tttcgtggtg aaagaacggg gtcattgcac ggagtttcga
     1861 ctgcgggaaa gcagagctgc cgttcacttc gtctataatt agcgctttct attttccccg
     1921 attcgggccg ctgctgcgct tttccgcctg ctgtttgtgg caagtgtagc agcaggctgt
     1981 gcacgcagtg tggcatgcac ttggctttcc accgttggta tcgattctct gggacgatga
     2041 gtcattcctt tcggggccac agcataatcg ttgccagctc accgaaatgg tgacttcatt
     2101 tcttaactgc cgtcaagcat gcgattgtac atacatacat atttatatat gtacatattt
     2161 atgtgactat ggtaggtcga tataatagca atcaacgcaa gcaaatgtgt cagtcctgct
     2221 tacaggaacg attctattta gtaattttcg ttgtataaag taattatgta tgtatgtaag
     2281 ccccataaat ctgaaacaat taggcaaaac catgcgaagc tctctagtat aacttcgtat
     2341 aatgtatgct atacgaagtt ctcgaggggg ggcccggtac cgtcgacgat gtaggtcacg
     2401 gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
     2461 acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt gatcccggcg
     2521 aacgcgcggc gcgccgggaa gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg
     2581 agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc
     2641 tcgacggtca cggcgggcat gtcgacacta gtggtaccca gcttttgttc cctttagtga
     2701 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2761 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2821 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2881 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2941 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3001 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3061 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3121 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3181 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3241 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3301 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3361 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3421 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3481 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3541 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3601 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3661 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3721 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3781 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3841 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3901 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3961 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4021 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4081 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4141 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4201 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4261 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4321 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4381 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4441 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4501 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4561 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4621 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4681 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4741 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4801 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4861 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4921 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4981 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5041 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5101 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5161 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5221 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5281 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     5341 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     5401 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     5461 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     5521 agggcgaatt ggagctccac cgcggtggcg gccgctttaa tatactcgaa ttcgttgtcg
     5581 gctcatatac atgggcgaaa taatttcgaa tatgttttaa aaataaccaa agacattaga
     5641 aagagatcgc caatacttat acattatgtc tatgtgtgcc atgtggtagc atgagccaaa
     5701 aagcttctcg aaattacgaa ttacatatag acgaatatgt atgtgacttt agtttcgaaa
     5761 taatttcgag aattttaaaa ataacgcatt cgttaaaagt tcgcgtcaat tcgaatcgaa
     5821 ttttcgattg tcgatttagt gtggattgtc gaaaatcgtt ccgccttcga agtttactga
     5881 aaggaatcat tgcgatctcg tgaattgctt gtatgagaac acgccaccat atcgagatac
     5941 ctgttcaaaa tatcgaaatc gcgttccaaa cggtagtagt taccagttga gtgagttgtg
     6001 gtgacagttt aacctctcta catttgtaat aataagcatt gttttggcgt atcaatagtc
     6061 tgtagatttt ttcacaaaga aacctggaga aatgtacaca aatatctaaa tgaaaattgg
     6121 cgttacgtaa caaataccaa cgccccgaaa atccg
//
