LOCUS       sequence-357873-        5611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  in vitro transcription of phiC31 integrase mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: T7-phiC31-nos3'UTR
FEATURES             Location/Qualifiers
     source          1..5611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(368..385)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(368..384)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(377..399)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(593..612)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     712..734
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(772..790)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        858..962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(963..1031,1032..1823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1181..1200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1994..2582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2483..2502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2736..2753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2870..2891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2906..2911,2912..2929,2930..2936)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2944..2960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2949..2971
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2968..2984
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2968..2984
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3058..3077
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     3161..3180
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3161..3179
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3180..3204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3219..3241
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3249..5066
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 atagcaagga acactttcgt cgtcggctac gcattcattg taaaatttta aattttgaca
       61 ttccgcactt tttgatagat aagcgaagag tatttttatt acatgtatcg caagtattca
      121 tttcaacaca catatctata tatatatgtt atatatttat tcaattttgt ttaccattga
      181 tcaatttttc acacatgaaa caaccgccag cattatataa tttttttatt tttttaaaaa
      241 atgtgtacac atattctgaa aatgaaaaat tcaatggctc gagtgccaaa taaagaaatg
      301 gttacaattt aaggaaacaa atgtccttct tgcgtttatg cattcgcgag gtaccgagct
      361 cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      421 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      481 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
      541 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
      601 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
      661 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      721 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
      781 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1201 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1801 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     3001 ttgcatgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
     3061 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
     3121 gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact cactataggg
     3181 gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag
     3241 atatacatat ggacacgtac gcgggtgctt acgaccgtca gtcgcgcgag cgcgaaaatt
     3301 cgagcgcagc aagcccagcg acacagcgta gcgccaacga agacaaggcg gccgaccttc
     3361 agcgcgaagt cgagcgcgac gggggccggt tcaggttcgt cgggcatttc agcgaagcgc
     3421 cgggcacgtc ggcgttcggg acggcggagc gcccggagtt cgaacgcatc ctgaacgaat
     3481 gccgcgccgg gcggctcaac atgatcattg tctatgacgt gtcgcgcttc tcgcgcctga
     3541 aggtcatgga cgcgattccg attgtctcgg aattgctcgc cctgggcgtg acgattgttt
     3601 ccactcagga aggcgtcttc cggcagggaa acgtcatgga cctgattcac ctgattatgc
     3661 ggctcgacgc gtcgcacaaa gaatcttcgc tgaagtcggc gaagattctc gacacgaaga
     3721 accttcagcg cgaattgggc gggtacgtcg gcgggaaggc gccttacggc ttcgagcttg
     3781 tttcggagac gaaggagatc acgcgcaacg gccgaatggt caatgtcgtc atcaacaagc
     3841 ttgcgcactc gaccactccc cttaccggac ccttcgagtt cgagcccgac gtaatccggt
     3901 ggtggtggcg tgagatcaag acgcacaaac accttccctt caagccgggc agtcaagccg
     3961 ccattcaccc gggcagcatc acggggcttt gtaagcgcat ggacgctgac gccgtgccga
     4021 cccggggcga gacgattggg aagaagaccg cttcaagcgc ctgggacccg gcaaccgtta
     4081 tgcgaatcct tcgggacccg cgtattgcgg gcttcgccgc tgaggtgatc tacaagaaga
     4141 agccggacgg cacgccgacc acgaagattg agggttaccg cattcagcgc gacccgatca
     4201 cgctccggcc ggtcgagctt gattgcggac cgatcatcga gcccgctgag tggtatgagc
     4261 ttcaggcgtg gttggacggc agggggcgcg gcaaggggct ttcccggggg caagccattc
     4321 tgtccgccat ggacaagctg tactgcgagt gtggcgccgt catgacttcg aagcgcgggg
     4381 aagaatcgat caaggactct taccgctgcc gtcgccggaa ggtggtcgac ccgtccgcac
     4441 ctgggcagca cgaaggcacg tgcaacgtca gcatggcggc actcgacaag ttcgttgcgg
     4501 aacgcatctt caacaagatc aggcacgccg aaggcgacga agagacgttg gcgcttctgt
     4561 gggaagccgc ccgacgcttc ggcaagctca ctgaggcgcc tgagaagagc ggcgaacggg
     4621 cgaaccttgt tgcggagcgc gccgacgccc tgaacgccct tgaagagctg tacgaagacc
     4681 gcgcggcagg cgcgtacgac ggacccgttg gcaggaagca cttccggaag caacaggcag
     4741 cgctgacgct ccggcagcaa ggggcggaag agcggcttgc cgaacttgaa gccgccgaag
     4801 ccccgaagct tccccttgac caatggttcc ccgaagacgc cgacgctgac ccgaccggcc
     4861 ctaagtcgtg gtgggggcgc gcgtcagtag acgacaagcg cgtgttcgtc gggctcttcg
     4921 tagacaagat cgttgtcacg aagtcgacta cgggcagggg gcagggaacg cccatcgaga
     4981 agcgcgcttc gatcacgtgg gcgaagccgc cgaccgacga cgacgaagac gacgcccagg
     5041 acggcacgga agacgtagcg gcgtagcgag acacccggat cagagggcga atccagctct
     5101 ggagcagagg ctctggcagc ttttgcagcg tttatataac atgaaatata tatacgcatt
     5161 ccgatcaaag ctgggttaac cagatagata gtaacgttta actagcgcct ggcgcgttcg
     5221 attttaaaga gatttagagc gttatcccgt gcctatagat cttatagtat agacaacgaa
     5281 cgatcactca aatccaagtc aataattcaa gaatttatgt ctgtttctgt gaaagggaaa
     5341 ctaattttgt taaagaagac ttacaatatc gtaatacttg ttcaatcgtc gtggccgata
     5401 gaaatatctt acaatccgaa agttgatgaa tggaattggt ctgcaactgg tcgccttcat
     5461 tttgtaaaat gttcgcttgc ggccgaaaaa tttcgatata tctacaattg atatacaatc
     5521 tttactaaat tttgaaaaag gaacactttg aatttcgaac tgtcaatcgt atcattagaa
     5581 tttaatctaa atttaaatct tgctaaagga a
//
