LOCUS       sequence-357874-        5560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter gene with yeast recombination site within 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: p{3P3-dsRed::FRT-RC,attB}
FEATURES             Location/Qualifiers
     source          1..5560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     561..580
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(799..903)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      930..1385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1017..1036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1227..1248
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1533
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1525..1542
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1526..1542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1552..1571
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1552..1570
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1603..1619
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1603..1619
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    2123..2147
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2164..2197)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(2218..2242)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3936..3969
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4010..4079
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(4298..4318)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4298..4316)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4337..4353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4337..4353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4350..4372)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4361..4377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4385..4391,4392..4409,4410..4415))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4430..4451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4568..4585)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4739..5327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4819..4838)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5498..5560,1..729,730..798))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
       61 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
      241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
      301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
      361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
      421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
      481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
      601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      901 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
      961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     1021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     1081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     1141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     1201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     1261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     1321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     1381 aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     1441 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     1501 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     1561 tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac tagtggatcc
     1621 cccggggatc taattcaatt agagactaat tcaattagag ctaattcaat taggatccaa
     1681 gcttatcgat ttcgaaccct cgaccgccgg agtataaata gaggcgcttc gtctacggag
     1741 cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa
     1801 acaagcgcag ctgaacaagc taaacaatcg ggcggccgca ctagagccgg tcgccaccat
     1861 gaggtcttcc aagaatgtta tcaaggagtt catgaggttt aaggttcgca tggaaggaac
     1921 ggtcaatggg cacgagtttg aaatagaagg cgaaggagag gggaggccat acgaaggcca
     1981 caataccgta aagcttaagg tgagtagtac atgtgcatac atcttaagtt cacttgatct
     2041 ataggaactg cgattgcaac atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa
     2101 tcccaaaccg cggccgttta tcaccacttt gtacaagaaa gctgggtcgg cgcgcccacc
     2161 cttgaagttc ctatactttc tagagaatag gaacttcggt gaagggggcg gccgcggagc
     2221 ctgctttttt gtacaaactt gtgataaacg aattcttgaa gacgaaaggg cacaaacaaa
     2281 tagtgacacg aaacagatta ttctggtagc tgttctcgct atataagaca atttttgaga
     2341 tcatatcatg atcaagacat ctaaaggcat tcattttcga ctatattctt ttttacaaaa
     2401 aatataacaa ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata
     2461 aacctacttc gtaggatact tcggggtact ttttgttcgg ggttagatga gcataacgct
     2521 tgtagttgat atttgagatc ccctatcatt gcaggtaacc aaggggggac ctttgccatt
     2581 tgcttgggat attttgtcac cacaatttca gtatggaagc aaggtatatg tcaagcaccc
     2641 tgccgacata ccagactata aaaagctgtc atttcctgaa ggatttaaat gggaaagggt
     2701 catgaacttt gaagacggtg gcgtcgttac tgtaacccag gattccagtt tgcaggatgg
     2761 ctgtttcatc tacaaggtca agttcattgg cgtgaacttt ccttccgatg gacctgttat
     2821 gcaaaagaag acaatgggct gggaagccag cactgagcgt ttgtatcctc gtgatggcgt
     2881 gttgaaagga gagattcata aggctctgaa gctgaaagac ggtggtcatt acctagttga
     2941 attcaaaagt atttacatgg caaagaagcc tgtgcagcta ccagggtact actatgttga
     3001 ctccaaactg gatataacaa gccacaacga agactataca atcgttgagc agtatgaaag
     3061 aaccgaggga cgccaccatc tgttccttta gcggccatcg aattcgagct cgcccactaa
     3121 gcgtcgcgcc acttcaacgc tcgatgggag cgtcattggt gggcggggta accgtcgaaa
     3181 tcagtgttta cgcttccaat cgcaacaaaa aattcactgc aacactgaaa agcatacgaa
     3241 aacgatgaag attgtacgag aaaccataaa gtattttatc cacaaagaca cgtatagcag
     3301 aaaagccaag ttaactcggc gataagttgt gtacacaaga ataaaatcgg ccagattcag
     3361 tgttgtcaga aataagaaaa ccccactatg tttttctttg ccttttcttt ctcccagcga
     3421 tcattcattt cgtggtgaaa gaacggggtc attgcacgga gtttcgactg cgggaaagca
     3481 gagctgccgt tcacttcgtc tataattagc gctttctatt ttccccgatt cgggccgctg
     3541 ctgcgctttt ccgcctgctg tttgtggcaa gtgtagcagc aggctgtgca cgcagtgtgg
     3601 catgcacttg gctttccacc gttggtatcg attctctggg acgatgagtc attcctttcg
     3661 gggccacagc ataatcgttg ccagctcacc gaaatggtga cttcatttct taactgccgt
     3721 caagcatgcg attgtacata catacatatt tatatatgta catatttatg tgactatggt
     3781 aggtcgatat aatagcaatc aacgcaagca aatgtgtcag tcctgcttac aggaacgatt
     3841 ctatttagta attttcgttg tataaagtaa ttatgtatgt atgtaagccc cataaatctg
     3901 aaacaattag gcaaaaccat gcgaagctct ctagtataac ttcgtataat gtatgctata
     3961 cgaagttctc gagggggggc ccggtaccgt cgacgatgta ggtcacggtc tcgaagccgc
     4021 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct
     4081 ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac gcgcggcgcg
     4141 ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg
     4201 cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg acggtcacgg
     4261 cgggcatgtc gacactagtg gtacccagct tttgttccct ttagtgaggg ttaattgcgc
     4321 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4381 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     4441 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     4501 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4561 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4621 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4681 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4741 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4801 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4861 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4921 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4981 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5041 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5101 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5161 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5221 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5281 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5341 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5401 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5461 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5521 cacctatctc agcgatctgt ctatttcgtt catccatagt
//
