LOCUS       sequence-357911-       13390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian co-expression of human SEPT2 fused to GFP10 and of human 
            SEPT2 fused to GFP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13390)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 13390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pTRIP TRE BI_SEPT2-14-GFP11_SEPT2-14-GFP10
FEATURES             Location/Qualifiers
     source          1..13390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    22..103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1430..1817
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     protein_bind    1511..1529
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1547..1565
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1582..1600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1618..1636
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1654..1672
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1689..1707
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1725..1743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3215..3232)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3442..3461
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3623..4324
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             4607..4787
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6315..6334)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6548..6567)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6667..6689
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6727..6745)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6813..6917
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6918..6986,6987..7778)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7136..7155)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7949..8537
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8438..8457
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8691..8708
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8778..8798)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8783..9112
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8963..9098
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10246..10311
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10441..10461
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10886..11020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     10977..10996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11188..11821
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11868..11993
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12486..12719
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12904..12948
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13241..13358
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttggctgata cgcgtacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
       61 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      181 ctacaaatgt ggtatggctg attatgatcc tctagattag gaggcgtcgg tgatgccggc
      241 ggcggtcacg tactccagca gcaccatgtg gtcgcgcttc tctccggagg acccaccacc
      301 tccagagcca ccgccaccat cgatcacatg gtggccgaga gccccgccgt cgccgtcacc
      361 accctgcatc tgcatctgca tctgcgcttg cattcttgca atcatctctt gcatgcggcg
      421 gagctcagcc tccttttcaa gcaagatctg gtctttattc atgtcctcat tctctacttt
      481 cctgccgcct ctcttcagcc tctcagaacg gaagttttca tagtgaaggt cttgggtcac
      541 ttcctgtagg tcctgcatgt gggtgatgag catcgttctc agcttcagaa agtcattgtg
      601 ttctgggttc tccacctcta caacacccca tgggtagaga cggcctctaa ccttcttgcc
      661 tttggcttca atcaactggt tggagccaac cacagagaat gggatactgg ccttgaggag
      721 tctagtctgc tccttaaagt cttcatcttc atctgactct gcatcaggta agtgatagat
      781 tttaatgtta tgctcttcaa tttcatccaa aatccttttc ttaagccgct cacgctcctt
      841 cagagtgaga gtgtcagctt tcgcaatgac aggcacaata ttcaccttat tgtgtatcgc
      901 tttcataaat gcaacatcta agggcttcag tccatgtcca aaaggtgaaa tgaagtagaa
      961 gcaacaatgt accctgttat caatgatgtg acgcctgttc agtccactct catcatgtag
     1021 gtagcgttca aactgctcat caatgtagga gataattgtc ttgaaacaat ccctgcagtt
     1081 gatggcatcc ccgtagccgg gagtgtccac tactgtaagc cgcagcttca caccccgctc
     1141 ttcaatctca acagtcgaag cctctatctg gacagttctt tcaattttct ctgcagctcc
     1201 aggaatcact ctttctgggt agagatcagt caggaataag ctgtttatga gagttgattt
     1261 tcctagacca gattcaccaa ccaccatcag agtgaactcg aaccccttct tcactgattt
     1321 tcggtgaact tgattgggaa gatttgcaaa tccaacatag ccaggagttt ctggatttat
     1381 aaactgagtt ggttgttgct tagacatcat atgaccggtg aattctccag gcgatctgac
     1441 ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccac ctcgacatac
     1501 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     1561 agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
     1621 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     1681 cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     1741 agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     1801 tgaaccgtca gatcgcctgg agatttcgag ctcggtaccg cggatgtcta agcaacaacc
     1861 aactcagttt ataaatccag aaactcctgg ctatgttgga tttgcaaatc ttcccaatca
     1921 agttcaccga aaatcagtga agaaggggtt cgagttcact ctgatggtgg ttggtgaatc
     1981 tggtctagga aaatcaactc tcataaacag cttattcctg actgatctct acccagaaag
     2041 agtgattcct ggagctgcag agaaaattga aagaactgtc cagatagagg cttcgactgt
     2101 tgagattgaa gagcggggtg tgaagctgcg gcttacagta gtggacactc ccggctacgg
     2161 ggatgccatc aactgcaggg attgtttcaa gacaattatc tcctacattg atgagcagtt
     2221 tgaacgctac ctacatgatg agagtggact gaacaggcgt cacatcattg ataacagggt
     2281 acattgttgc ttctacttca tttcaccttt tggacatgga ctgaagccct tagatgttgc
     2341 atttatgaaa gcgatacaca ataaggtgaa tattgtgcct gtcattgcga aagctgacac
     2401 tctcactctg aaggagcgtg agcggcttaa gaaaaggatt ttggatgaaa ttgaagagca
     2461 taacattaaa atctatcact tacctgatgc agagtcagat gaagatgaag actttaagga
     2521 gcagactaga ctcctcaagg ccagtatccc attctctgtg gttggctcca accagttgat
     2581 tgaagccaaa ggcaagaagg ttagaggccg tctctaccca tggggtgttg tagaggtgga
     2641 gaacccagaa cacaatgact ttctgaagct gagaacgatg ctcatcaccc acatgcagga
     2701 cctacaggaa gtgacccaag accttcacta tgaaaacttc cgttctgaga ggctgaagag
     2761 aggcggcagg aaagtagaga atgaggacat gaataaagac cagatcttgc ttgaaaagga
     2821 ggctgagctc cgccgcatgc aagagatgat tgcaagaatg caagcgcaga tgcagatgca
     2881 gatgcagggt ggtgacggcg acggcggggc tctcggccac catgtgatcg atggtggcgg
     2941 tggctctgga ggtggtgggt cctccggaat gggcgacctg cccgacgacc actacctgtc
     3001 cacccagacc atcctgagca aggacctgaa ctaagctagc taatagttcc gcccctctcc
     3061 ctccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     3121 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     3181 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     3241 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     3301 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     3361 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     3421 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     3481 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     3541 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     3601 ctttgaaaaa cacgatgcaa gcatgagcga gctgattaag gagaacatgc acatgaagct
     3661 gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa
     3721 gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt
     3781 cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac
     3841 ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt
     3901 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
     3961 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat
     4021 gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg
     4081 cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa
     4141 catcaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta
     4201 ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca
     4261 gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa
     4321 ttaaggatcc agcggccgct cgagacctag aaaaacatgg agcaatcaca agtagcaata
     4381 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     4441 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     4501 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaaa
     4561 atcgtcgaga gatgctgcat ataagcagct gctttttgct tgtactgggt ctctctggtt
     4621 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     4681 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     4741 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     4801 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     4861 ttgacattat aatagattta gcaggaattg aactaggagt ggagcacaca ggcaaagctg
     4921 cagaagtact tggaagaagc caccagagat actcacgatt ctgcacatac ctggctaatc
     4981 ccagatccta aggattacat taagtttact aacatttata taatgattta tagtttaaag
     5041 tataaactta tctaatttac tattctgaca gatattaatt aatcctcaaa tatcataaga
     5101 gatgattact attatcccca tttaacacaa gaggaaactg agagggaaag atgttgaagt
     5161 aattttccca caattacagc atccgttagt tacgactcta tgatcttctg acacaaattc
     5221 catttactcc tcaccctatg actcagtcga atatatcaaa gttatggaca ttatgctaag
     5281 taacaaatta cccttttata tagtaaatac tgagtagatt gagagaagaa attgtttgca
     5341 aacctgaata gcttcaagaa gaagagaagt gaggataaga ataacagttg tcatttaaca
     5401 agttttaaca agtaacttgg ttagaaaggg attcaaatgc ataaagcaag ggataaattt
     5461 ttctggcaac aagactatac aatataacct taaatatgac ttcaaataat tgttggaact
     5521 tgataaaact aattaaatat tattgaagat tatcaatatt ataaatgtaa tttactttta
     5581 aaaagggaac atagaaatgt gtatcattag agtagaaaac aatccttatt atcacaattt
     5641 gtcaaaacaa gtttgttatt aacacaagta gaatactgca ttcaattaag ttgactgcag
     5701 attttgtgtt ttgttaaaat tagaaagaga taacaacaat ttgaattatt gaaagtaaca
     5761 tgtaaatagt tctacatacg ttcttttgac atcttgttca atcattgatc gaagttcttt
     5821 atcttggaag aatttgttcc aaagactctg aaataaggaa aacaatctat tatatagtct
     5881 cacacctttg ttttactttt agtgatttca atttaataat gtaaatggtt aaaatttatt
     5941 cttctctgag atcatttcac attgcagata gaaaacctga gactggggta atttttatta
     6001 aaatctaatt taatctcaga aacacatctt tattctaaca tcaatttttc cagtttgata
     6061 ttatcatata aagtcagcct tcctcatctg caggttccac aacaaaaatc caaccaactg
     6121 tggatcaaaa atattgggaa aaaattaaaa atagcaatac aacaataaaa aaatacaaat
     6181 cagaaaaaca gcacagtata acaactttat ttagcattta caatctatta ggtattataa
     6241 gtaatctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     6301 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     6361 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     6421 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     6481 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca
     6541 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     6601 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     6661 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     6721 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6781 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6841 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6901 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6961 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7021 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7081 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7141 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7201 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7261 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7321 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7381 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7441 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7501 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7561 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7621 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7681 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7741 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7801 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7861 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7921 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7981 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8041 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8101 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8161 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8221 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8281 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8341 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8401 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8461 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8521 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8581 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8641 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8701 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8761 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8821 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8881 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8941 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9001 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9061 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     9121 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     9181 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     9241 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     9301 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     9361 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     9421 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     9481 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     9541 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     9601 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     9661 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     9721 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     9781 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     9841 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     9901 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     9961 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    10021 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
    10081 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
    10141 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    10201 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
    10261 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
    10321 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
    10381 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
    10441 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
    10501 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
    10561 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
    10621 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
    10681 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
    10741 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
    10801 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    10861 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    10921 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10981 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    11041 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    11101 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    11161 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
    11221 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
    11281 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
    11341 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
    11401 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
    11461 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
    11521 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
    11581 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
    11641 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
    11701 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
    11761 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
    11821 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
    11881 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
    11941 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
    12001 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
    12061 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
    12121 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
    12181 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
    12241 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
    12301 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
    12361 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    12421 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    12481 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    12541 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    12601 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    12661 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    12721 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    12781 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    12841 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    12901 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    12961 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    13021 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    13081 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    13141 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    13201 cggtatcgcc gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    13261 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    13321 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    13381 gagatccact
//
