LOCUS       sequence-357915-       11086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to epress MCP-mCherry fusion protein in the early fly embryos 
            (driven by maternal nanos driver).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Liu J, Hansen D, Eck E, Kim YJ, Turner M, Alamos S, Garcia HG
  TITLE     Real-time single-cell characterization of the eukaryotic 
            transcription cycle reveals correlations between RNA initiation, 
            elongation, and cleavage.
  JOURNAL   PLoS Comput Biol. 2021 May 18;17(5):e1008999. doi: 
            10.1371/journal.pcbi.1008999. eCollection 2021 May.
  PUBMED    34003867
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2021-05-18"; volume: "17(5):e1008999. doi"; pages: " 
            10.1371/journal.pcbi.1008999. eCollection 2021 May"
COMMENT     Sequence Label: pCasper-pNos-MCP-mCherry
FEATURES             Location/Qualifiers
     source          1..11086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      260..848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     749..768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(1083..1113,1114..1668)
                     /label=P element 5' end
     CDS             complement(join(2447..3061,3132..3263,3467..3782,3844..4498,
                     4573..4846,5248..5319))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     5771..5789
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     5818..5834
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5819..5835
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7144..7851
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7286..7304)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7554..7573
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8677..8694)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8677..8693)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8686..8708)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    join(9175..9376,9377..9407)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        10210..10314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10315..10383,10384..11086,1..89)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10533..10552)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
       61 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
      121 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
      181 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
      241 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      301 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      361 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      421 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      481 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      541 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      601 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      661 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      721 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      781 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      841 tatggaaaaa cgccttcttc ttgaactcgg gctcggtgcc agtatacctc aaatggttgt
      901 cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa tgggcggacg
      961 tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg
     1021 tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac gtgggtctgg
     1081 cccatgatga aataacataa ggtggtcccg tcgatagccg aagcttaccg aagtatacac
     1141 ttaaattcag tgcacgtttg cttgttgaga ggaaaggttg tgtgcggacg aatttttttt
     1201 tgaaaacatt aacccttacg tggaataaaa aaaaatgaaa tattgcaaat tttgctgcaa
     1261 agctgtgact ggagtaaaat taattcacgt gccgaagtgt gctattaaga gaaaattgtg
     1321 ggagcagagc cttgggtgca gccttggtga aaactcccaa atttgtgata cccactttaa
     1381 tgattcgcag tggaaggctg cacctgcaaa aggtcagaca tttaaaagga ggcgactcaa
     1441 cgcagatgcc gtacctagta aagtgataga gcctgaacca gaaaagataa aagaaggcta
     1501 taccagtggg agtacacaaa cagagtaagt ttgaatagta aaaaaaatca tttatgtaaa
     1561 caataacgtg actgtgcgtt aggtcctgtt cattgtttaa tgaaaataag agcttgaggg
     1621 aaaaaattcg tactttggag tacgaaatgc gtcgtttaga gcagcagccg aattaattct
     1681 agttccagtg aaatccaagc attttctaaa ttaaatgtat tcttattatt atagttgtta
     1741 tttttgatat atataaacaa cactattatg cccaccattt ttttgagatg catctacaca
     1801 aggaacaaac actggatgtc actttcagtt caaattgtaa cgctaatcac tccgaacagg
     1861 tcacaaaaaa ttaccttaaa aagtcataat attaaattag aataaatata gctgtgaggg
     1921 aaatatatac aaatatattg gagcaaataa attgtacata caaatattta ttactaattt
     1981 ctattgagac gaaatgaacc actcggaacc atttgagcga accgaatcgc gcggaactaa
     2041 cgacagtcgc tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag cgggtgggag
     2101 acagcgaaag agcaactacg aaacgtggtg tggtggaggt gaattatgaa gagggcgcgc
     2161 gatttgaaaa gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg ataaacgagt
     2221 ttttgatgta aggtatgcag gtgtgtaagt cttttggtta gaagacaaat ccaaagtcta
     2281 cttgtgggga tgttcgaagg ggaaatactt gtattctata ggtcatatct tgtttttatt
     2341 ggcacaaata taattacatt agctttttga gggggcaata aacagtaaac acgatggtaa
     2401 taatggtaaa aaaaaaaaac aagcagttat ttcggatata tgtcggctac tccttgcgtc
     2461 gggcccgaag tcttagagcc agatatgcga gcacccggaa gctcacgatg agaatggcca
     2521 gacccacgta gtccagcggc agatcggcgg cggagaagtt aagcgtctcc aggatgacct
     2581 tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc ggctccacgt
     2641 ccgcccattg gttaatcagc agaccctcgt tggcgtaacg gaaccatgag aggtacgaca
     2701 accatttgag gtatactggc accgagcccg agttcaagaa gaagccgcca aagagcagga
     2761 atggtatgat aaccggcgga cccacagaca gcgccatcga ggtcgaggag ctggcgcagg
     2821 atattagata tccgaaggac gttgacacat tggccaccag agtgaccagc gccaggcagt
     2881 tgaagaagtg cagcactccg gcccgcagtc cgatcatcgg ataggcaatc gccgtgaaga
     2941 ccagtggcac tgtgagaaaa agcggcaatt cggcaatcgt tttgcccaga aagtatgtgt
     3001 cacagcgata aagtcgactt cgggcctccc tcataaaaac tggcagctct gaggtgaaca
     3061 cctaaatcga atcgattcat tagaaagtta gtaaattatt gaaatgcaaa tgtattctaa
     3121 acatgactta catttatcgt ggcaaagacg ttttgaaagg tcatgttggt caggaagagg
     3181 aagatggctc cgttgatatt catcacaccc acttgcgtga gttgttggcc caaaaagatg
     3241 aggccaatca agatggcaac catctgcaaa ttaaaatgtt actcgcatct cattaatatt
     3301 cgcgagttaa atgaaattta tttatcttct gcaaaactat aaactataca tctcattgaa
     3361 aaaaactaag aagggtgtgg aatcaggcaa ttctatctaa aatctagcga atttgtttcc
     3421 aagaattgta agcgttatat catttgtttc cactggaacc actcaccgtt gtctgaataa
     3481 gtcgcacttt tacgaggagt ggttccttga gcaccgacag ccaggatcgc cacaggaccg
     3541 cccggaactg catgaaccag gtggccttgt aggtgtaccc attctccggc tgctccagtg
     3601 gcttctccag atttttggtg gccaacaact gctccatatc ccgggctact ttgctaatgg
     3661 caaaattgtc gcatatcttg gcgatccgat cacgggactc gatctcccgt ccgggcacaa
     3721 cggccaacac ctgtacgtaa aagtccgccg gattgtagtt ggtaggacac tgggcaccca
     3781 cgctggatag gagttgagat gttatgtaat actagatacc cttaataaac acatcgaact
     3841 cactaggaaa agaagtcgac ggcttcgctg ggagtgccca agaaagctac cctgccctcg
     3901 gccatcagaa ggatcttgtc aaagagctca aacagctcgg aagacggctg atgaatggtc
     3961 aggatgacgg tcttgccctt ctgcgacagc ttcttcagca cctggacgac gctgtgggcg
     4021 gtaaaggagt ccagtccgga ggtgggctca tcgcagatca gaagcggcgg atcggttaga
     4081 gcctcggagg cgaatgccag acgcttcctt tctccgccgg acagaccttt caccctgccg
     4141 ggcacaccga tgatcgtgtg ctgacatttg ctgagcgaaa gctcctggat cacctgatcc
     4201 acgcgggcca ctcgctgccg ataggtcaga tgtcgtggca tccgcaccat ggcttggaaa
     4261 atcaggtgtt ccctggccgt tagggagccg ataaagaggt catcctgctg gacataggcg
     4321 cacctggcct gcatctcctt ggcgtccaca ggttggccat tgagcagtcg catcccggat
     4381 ggcgatactt ggatgccctg cggcgatcga aaggcaaggg cattcagcag ggtcgtcttt
     4441 ccggcaccgg aactgcccat cacggccaaa agttcgcccg gataggccac gccgcaaact
     4501 gagtttcaaa ttggtaattg gaccctttat taagatttca cacagatcag ccgactgcga
     4561 atagaaactc accgttcttg agcaaatgtt tcctgggcgc cggtatgtgt cgctcgttgc
     4621 agaatagtcc gcgtgtccgg ttgaccagct gccgccatcc ggagcccggc tgattgaccg
     4681 ccccaaagat gtccatattg tgccaggcat aggtgaggtt ctcggctagt tggccgctcc
     4741 ctgaaccgga gtcctccggc ggactgggtg gccggagcgt gccgtagttt ttggcctgcc
     4801 cgaagccctg gttaatgcag ctctgcgaag ccgctccgct gtcaccctgc aatgataggg
     4861 gatctcaaat atcaactaca agcgttatgc tcatctaacc ccgaacaaaa agtaccccga
     4921 agtatcctac gaagtaggtt tatactttta tttatttttt gtgcatctag gatcagctta
     4981 aaatatctgg ttgttatatt ttttgtaaaa aagaatatag tcgaaaatga atgcctttag
     5041 atgtcttgat catgatatga tctcaaaaat tgtcttatat agcgagaaca gctaccagaa
     5101 taatctgttt cgtgtcacta tttgtttgtg caattgcggt ttgggatttt tgtgggtcgc
     5161 agttctcacg ccgcagacaa tttgatgttg caatcgcagt tcctatagat caagtgaact
     5221 taagatgtat gcacatgtac tactcacatt gttcagatgc tcggcagatg ggtgtttgct
     5281 gcctccgcga attaatagct cctgatcctc ttggcccatt gccgggattt ttcacacttt
     5341 cccctgctta cccacccaaa accaatcacc accccaatca ctcaaaaaac aaacaaaaat
     5401 aagaagcgag aggagttttg gcacagcact ttgtgtttaa ttgatggcgt aaaccgcttg
     5461 gagcttcgtc acgaaaccgc tgacaaaatg caactgaagg cggacattga cgctacgtaa
     5521 cgctacaaac ggtggcgaaa gagatagcgg acgcagcggc gaaagagacg gcgatatttc
     5581 tgtggacaga gaaggaggca aacagcgctg actttgagtg gaatgtcatt ttgagtgaga
     5641 ggtaatcgaa agaacctggt acatcaaata cccttggatc gaagtaaatt taaaactgat
     5701 cagataagtt caatgatatc cagtgcagta aaaaaaaaaa atgttttttt tatctacttt
     5761 ccgcaaaaat gggttttatt aacttacata catactagaa ttcggtaccc ggggatctcg
     5821 aggtcgacgg tatcgataag cttcgaccgt tttaacctcg aaatatgcac atgtaaggac
     5881 ggatgtgagc gaacgccagt gatgaccggg atcagaggta acctaccatg gtggggatta
     5941 ggtgaccgtt cgcaggtagt ttgatcggag cgaatgttcg gggggtctgg cgtcagaggc
     6001 tctaaacttt atgtaattcc tgccgcgaaa cacgcacgta tcaagcagtc agctgttctc
     6061 ttcgttcagc gcgcgccggt gttgcaaaac gagcgctctt cgccggcggt ggctcgtgcg
     6121 atagttcgtt ttgtcggtaa tccgatgttg ccgcgccgat atcatgtgat gttgtcacag
     6181 tgcgcgaaat tcgaatggtg gtgtgcagtg attgtgttgt gacggcgagt ggcgcgtgtg
     6241 ggtgcttagt tttgggagat gttttcgtat ttttttgttg ataactcagg ctttgttgct
     6301 gtgttgtagt actattttcc attgcgcggt gtccagcttt taattagtgg cacatattct
     6361 tagcaagtaa aaattatttt gcatactatt aaatttctta taaattattt tctaaaatta
     6421 agtttacctt ttcaatttta ctaaaaatat cgatatattt attatcgctg gaaaactaca
     6481 ttattccacc tctaagcaag aaccgttagt tggcgcgtag ctttaccaca aaattcctgg
     6541 aattgccgta cgcttcgcag ttgtttcaag ttgtctaagg gacatacgat tttttttgcc
     6601 tctgcgtcac gattttaacc caaaagcgag tttagttaca tgtacattat tattagataa
     6661 agaagtatcg cgaatacttc agttgaataa actgtgcttg gtttttgggt gaggatttgt
     6721 ggaaagtaga gtgcgcgata accgtaactt tcgacccgga ttttccatag atcatgcatc
     6781 accatggctt ctaactttac tcagttcgtt ctcgtcgaca atggcggaac tggcgacgtg
     6841 actgtcgccc caagcaactt cgctaacggg atcgctgaat ggatcagctc taactcgcgt
     6901 tcacaggctt acaaagtaac ctgtagcgtt cgtcagagct ctgcgcagaa tcgcaaatac
     6961 accatcaaag tcgaggtgcc taaaggcgcc tggcgttcgt acttaaatat ggaactaacc
     7021 attccaattt tcgccacgaa ttccgactgc gagcttattg ttaaggcaat gcaaggtctc
     7081 ctaaaagatg gaaacccgat tccctcagca atcgcagcaa actccggcat ctacgcggcc
     7141 gctgtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     7201 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     7261 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     7321 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     7381 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     7441 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     7501 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     7561 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     7621 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     7681 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     7741 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     7801 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta aaccggcaca
     7861 ctaagcgtcg cgccacttca acgctcgatg ggagcgtcat tggtgggcgg ggtaaccgtc
     7921 gaaatcagtg tttacgcttc caatcgcaac aaaaaattca ctgcaacact gaaaagcata
     7981 cgaaaacgat gaagattgta cgagaaacca taaagtattt tatccacaaa gacacgtata
     8041 gcagaaaagc caagttaact cggcgataag ttgtgtacac aagaataaaa tcggccagat
     8101 tcagtgttgt cagaaataag aaaaccccac tatgtttttc tttgcctttt ctttctccca
     8161 gcgatcattc atttcgtggt gaaagaacgg ggtcattgca cggagtttcg actgcgggaa
     8221 agcagagctg ccgttcactt cgtctataat tagcgctttc tattttcccc gattcgggcc
     8281 gctgctgcgc ttttccgcct gctgtttgtg gcaagtgtag cagcaggctg tgcacgcagt
     8341 gtggcatgca cttggctttc caccgttggt atcgattctc tgggacgatg agtcattcct
     8401 ttcggggcca cagcataatc gttgccagct caccgaaatg gtgacttcat ttcttaactg
     8461 ccgtcaagca tgcgattgta catacataca tatttatata tgtacatatt tatgtgacta
     8521 tggtaggtcg atataatagc aatcaacgca agcaaatgtg tcagtcctgc ttacaggaac
     8581 gattctattt agtaattttc gttgtataaa gtaattatgt atgtatgtaa gccccataaa
     8641 tctgaaacaa ttaggcaaaa ccatgcgaag cttggcactg gccgtcgttt tacaacgtcg
     8701 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     8761 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     8821 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     8881 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     8941 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     9001 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     9061 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     9121 aataatggtt tcttagacaa ctcgaggcct cgaggtcgac ctgcagccaa gctttgcgta
     9181 ctcgcaaatt attaaaaata aaactttaaa aataatttcg tctaattaat attatgagtt
     9241 aattcaaacc ccacggacat gctaagggtt aatcaacaat catatcgctg tctcactcag
     9301 actcaatacg acactcagaa tactattcct ttcactcgca cttattgcaa gcatacgtta
     9361 agtggatgtc tcttgccgac gggaccacct tatgttattt catcatggtc tggccattct
     9421 catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga
     9481 gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta ttaccatcgt
     9541 gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca
     9601 agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg
     9661 atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat
     9721 cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc
     9781 ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg
     9841 ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc
     9901 gcgattcggt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     9961 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
    10021 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
    10081 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
    10141 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
    10201 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    10261 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    10321 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    10381 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    10441 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    10501 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
    10561 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    10621 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    10681 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10741 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10801 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    10861 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    10921 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    10981 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    11041 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtag
//
