LOCUS       sequence-357916-       12856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Nav1.6 tagged with 3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12856)
  AUTHORS   Liu H, Wang HG, Pitt GS, Liu ZJ
  TITLE     Direct Observation of Compartment-Specific Localization and Dynamics
            of Voltage-Gated Sodium Channels.
  JOURNAL   J Neurosci. 2022 Jun 6;42(28):5482-98. doi: 
            10.1523/JNEUROSCI.0086-22.2022.
  PUBMED    35672149
REFERENCE   2  (bases 1 to 12856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0086-22"; journalName: "J Neurosci"; date: 
            "2022-06-6- 6"; volume: "42"; issue: "28"; pages: "5482-98"
COMMENT     Sequence Label: piggybac-Syn-mScn8a-3xHA
FEATURES             Location/Qualifiers
     source          1..12856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6460..6486
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6502..6528
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6502..6528
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6544..6570
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6586..7158
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6753..6770)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6980..6999
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7167..7961
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7221..7240)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7831..7850
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8021..8041)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    8083..8204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8120..8139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8174..8193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8967..9001
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9492..9508)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9492..9508)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9505..9527)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9516..9532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9540..9546,9547..9564,9565..9570))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9585..9606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9723..9740)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9894..10482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9974..9993)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10653..11444,11445..11513))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11276..11295
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11514..11618)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11644..12099)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11781..11802)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11993..12012
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12226..12248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12240..12257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12241..12257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   12387..12449
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ggcctccacg gccaattcca tatgtgacga ccgaccccga cccactggac aagcacccaa
       61 cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac aggatgcggc
      121 gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc ccgcctggcg
      181 gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc
      241 aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca gccggaccgc
      301 accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg
      361 actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc
      421 agctgtgctc ctgggcaccg cgcagtccgc ccccgcggct cctggccaga ccacccctag
      481 gaccccctgc cccaagtcgc agccacgcgt atggcagcgc ggctgctcgc accaccaggc
      541 cccgacagtt tcaagccttt cacccccgag tcgctggcaa acatcgagag gcgcatcgcc
      601 gagagcaagc tcaagaaacc acccaaggcg gatggcagcc accgggagga cgatgaagac
      661 agcaagccca agccaaacag tgacctggag gcggggaaga gtttgccttt catctacggg
      721 gacatcccgc aaggcctggt tgcggttccc ctggaggact ttgacccgta ctatttgacg
      781 cagaaaactt ttgtagtatt aaacagaggg aaaactctct tcagatttag cgccactcct
      841 gccttgtaca ttctaagtcc ttttaacctg ataagaagaa tagctattaa aattttgata
      901 cattcagtct tcagcatgat catcatgtgc accatcctga ccaactgtgt attcatgact
      961 tttagtaacc ctcccgaatg gtccaagaat gtggagtaca cattcacagg gatttacaca
     1021 tttgaatcac tggtgaaaat catcgccaga ggtttctgca tagacggctt caccttctta
     1081 cgagacccgt ggaactggtt agacttcagt gtcatcatga tggcgtatat aacagaattc
     1141 gtaaacctag gcaatgtttc agctctacgc actttcaggg tactgagggc tttgaaaact
     1201 atttcggtaa tcccaggcct gaagacaatc gtgggcgccc tcatccagtc tgtgaagaag
     1261 ctgtcagacg tgatgatcct gacggtgttc tgcctgagtg tcttcgccct gattggcctg
     1321 cagctcttca tggggaacct tcgaaacaag tgtgtcgtgt ggcccataaa cttcaacgag
     1381 agctatctgg agaacggcac cagaggcttc gactgggagg agtatatcaa caataaaaca
     1441 aacttttaca tggtccctgg catgctagaa cccttgctct gcgggaacag ctctgacgcc
     1501 gggcaatgcc cagaggggtt ccagtgcatg aaagcaggaa gaaatcccaa ctacggctac
     1561 accagcttcg acaccttcag ctgggccttc ctggcactgt tccgcctcat gactcaggac
     1621 tactgggaga acttatacca gctgacctta cgagcggctg ggaaaacgta catgatcttc
     1681 tttgtcttgg tgatcttcgt gggttctttc tatttggtga acttgatcct ggctgtggtg
     1741 gccatggctt acgaagagca gaaccaggca acactggagg aggcagagca gaaagaggca
     1801 gagttcaagg cgatgctcga gcagctcaag aagcagcagg aggaggcgca ggctgctgca
     1861 atggccactt cggcgggcac cgtctcagaa gatgccattg aagaagaagg ggaagatggg
     1921 gtaggctctc cgaggagctc atctgagctg tctaaactca gttccaagag cgcgaaggag
     1981 cgtcggaacc gacggaagaa gaggaagcag aaggagctct ctgaagggga ggagaagggg
     2041 gaccccgaga aggtgtttaa gtcagagtcg gaagatggca tgagaaggaa ggccttccgg
     2101 ctgccagaca ataggatagg gaggaaattt tccatcatga atcagtcgct gctcagcatc
     2161 ccaggctcgc ccttcctttc ccgacacaac agcaaaagca gcatcttcag ctttcggggt
     2221 cctggacggt tccgggaccc cggttcggag aacgagttcg cagacgacga gcacagcaca
     2281 gtggaggaaa gtgagggccg gcgcgactcg ctcttcatcc cgatccgcgc ccgcgagcgc
     2341 cgcagcagct actctggata ctcaggatac agtcagtgca gccgctcgtc gcgcatcttc
     2401 cccagcctgc ggcgcagcgt gaaacgcaac agcaccgtgg actgcaatgg cgtggtgtcg
     2461 ctcatcgggc ctggctcgca catcgggcgg ctcctgcctg aggcaacaac tgaggtggag
     2521 attaagaaga aaggccctgg atcgctctta gtctccatgg aacaactcgc ctcctacgga
     2581 cggaaggaca gaatcaacag tataatgagc gttgtcacaa acacactggt ggaagagctg
     2641 gaagagtctc agagaaagtg cccaccatgc tggtataagt ttgccaatac tttcctcatc
     2701 tgggagtgtc acccctactg gataaaactg aaggagattg tgaacctgat cgtcatggac
     2761 cctttcgtag acttagccat aaccatctgc attgtcctga acacactatt tatggcaatg
     2821 gagcaccatc ccatgacgcc acaattcgaa catgtcctgg ccgtagggaa cctggtgttc
     2881 accggtatct tcacggcgga aatgttcctg aagctcatag ccatggatcc ctactattac
     2941 ttccaagaag gctggaacat ttttgacgga tttatcgtct ccctcagttt aatggagctg
     3001 ggccttgcag acgtggaggg gctctcagtg ctgcgatctt tccgattgct ccgagtcttc
     3061 aaattggcca agtcctggcc caccctgaac atgctgatca agatcattgg gaactccgtt
     3121 ggcgccctgg gcaacctgac cctggtgctg gccatcattg tcttcatctt tgccgtggtg
     3181 gggatgcagc tctttggaaa gagctacaag gagtgcgtct gtaagatcag ccaggagtgc
     3241 aagctcccgc gctggcacat gaacgacttc ttccactcct tcctcatcgt cttccgagtg
     3301 ctgtgtgggg agtggatcga gaccatgtgg gattgcatgg aagtggccgg ccaggccatg
     3361 tgccttatcg tgttcatgat ggtcatggtg attgggaacc tggtggtgct gaacttattt
     3421 ctggccttgc ttctgagctc cttcagcgca gacaatctgg cggccacgga cgacgacggg
     3481 gaaatgaaca acctgcagat atcggtgatc cggatcaaga agggcgtggc ctgggccaaa
     3541 gtgaaggtgc atgccttcat gcaggcacac ttcaagcaac gggaggctga tgaagtgaaa
     3601 cctttagacg agctgtatga gaagaaggcc aactgcatcg ccaaccacac cggcgtggac
     3661 attcacagga acggcgactt ccagaaaaat ggaaatggca ccactagtgg catcggcagc
     3721 agcgtggaga agtacatcat cgacgaggac cacatgtcct tcattaacaa cccaaacctg
     3781 accgtgcggg tgcccattgc tgtgggcgag tctgatttcg agaacctcaa cacagaggat
     3841 gttagcagcg agtcagaccc cgaaggcagc aaagacaaac tggacgatac cagctcctca
     3901 gaagggagta ccatcgacat caagcctgag gtggaagaag tcccagtgga gcagcctgag
     3961 gaatacttgg atccagacgc ctgcttcacg gagggttgtg tccagaggtt caagtgctgc
     4021 caggtcaaca tcgaggaagg actaggcaag tcgtggtgga tcctgcggaa aacctgcttc
     4081 ctcattgtgg agcacaattg gtttgagacc ttcatcattt tcatgattct cctcagcagt
     4141 ggagccctgg ccttcgagga catctacatt gagcagagga agaccatccg taccatcctg
     4201 gagtatgcgg acaaggtctt cacctacatc ttcatcctgg agatgttgct caaatggaca
     4261 gcctatggct tcgtcaagtt cttcaccaat gcctggtgct ggttggactt cctcattgtg
     4321 gctgtctctt tagtcagcct tatagctaat gccctgggct actcggaact aggtgccata
     4381 aagtccctta ggaccctaag agctttgaga cccttaagag ccttatcacg atttgaaggg
     4441 atgagggtgg tggtgaacgc cttggtgggc gccatcccct ctattatgaa cgtgctgctg
     4501 gtgtgtctca tcttctggct gattttcagc atcatgggcg ttaacctgtt tgctgggaaa
     4561 taccactact gcttcaacga gacttcagaa atccggttcg aaatcgacga ggtgaacaat
     4621 aaaaccgact gtgagaagct catggagggc aacaacacag agatccgatg gaagaacgtc
     4681 aagatcaact tcgacaacgt cggggcaggc tacctggccc ttcttcaagt ggcaaccttc
     4741 aaaggctgga tggacatcat gtatgcagct gtagattcta gaaagccgga cgagcagcct
     4801 gattatgagg gcaacatcta catgtacatc tacttcgtca tcttcatcat cttcggctcc
     4861 ttcttcaccc tcaacctgtt catcggtgtc atcatcgaca atttcaatca acagaagaaa
     4921 aagtttggag gtcaggacat cttcatgaca gaggaacaga agaagtacta caacgccatg
     4981 aagaagctag gctccaagaa gccacagaag cccatccccc gacctttgaa caaaatccaa
     5041 gggattgtct ttgatttcgt cacgcaacaa gccttcgaca tcgtgatcat gatgcttatc
     5101 tgcctcaaca tggtgaccat gatggtggag acagacacac agagcaagca gatggagaac
     5161 attctctact ggattaatct ggtcttcgtc atcttcttca cctgcgagtg tgtgctcaaa
     5221 atgtttgcct tgagacacta ctatttcacc attggctgga acatctttga ctttgtggtg
     5281 gtcattctct ccattgtggg aatgttcctg gctgatatca ttgagaagta cttcgtctcc
     5341 ccgaccctat tccgcgtcat ccgattggcc cgcatcgggc gcatcttgcg tctgatcaag
     5401 ggcgccaaag ggatccgcac cctgctcttt gccttaatga tgtcgctgcc cgccctgttc
     5461 aacatcggcc tcctgctctt cctggtcatg ttcatcttct ccatctttgg gatgtccaac
     5521 ttcgcgtacg tgaagcacga ggccggcatc gatgacatgt tcaacttcga gacgtttggc
     5581 aacagcatga tctgcctgtt ccagatcacg acctctgctg gttgggatgg cttactgctg
     5641 ccaatcctga accgcccccc tgactgcagc ttggacaagg agcacccagg aagtggcttc
     5701 aaaggggact gcgggaaccc ctccgtgggc atcttcttct tcgtcagcta catcatcatc
     5761 tcctttctga tcgtggtaaa catgtacatt gccatcatct tggagaactt cagcgtagcc
     5821 acagaggaaa gcgccgatcc tctgagcgag gacgacttcg agactttcta tgagatctgg
     5881 gagaagtttg atcctgatgc cacccagttc atcgagtact gtaagctggc cgactttgcc
     5941 gacgccctgg agcatccgct ccgagtaccc aagcccaaca ccatcgagct catcgccatg
     6001 gacctgccca tggtgagcgg agatcgaatc cactgcttgg acatcctttt cgccttcacc
     6061 aagcgagtcc tgggagacag tggggagttg gacatcctgc ggcagcagat ggaggagcgg
     6121 ttcgtggcta gcaatccttc caaagtgtct tacgagccta tcacgaccac tctgcggcgc
     6181 aagcaggaag aggtgtctgc agtggtcttg cagcgtgctt acaggggaca cctggctagg
     6241 cggggcttca tctgcagaaa gatcacttcc aacaagctgg agaatggagg cacccaccga
     6301 gagaagaagg agagcacccc gtccacagcc tccctcccct cttacgacag cgtgacaaag
     6361 cccgacaagg agaagcagca gcgggcagag gaaggcagga gggaaagagc caagaggcaa
     6421 aaagaagtca gggagtccaa gtgcggttct ggatcgggtt acccatacga tgttccagat
     6481 tacgctggca gtggtagcgg ttacccctac gacgtgcccg actacgccgg ctccggcagt
     6541 ggttatcctt atgacgtccc tgattacgcc tagtaagcgg ccgcgcccct ctccctcccc
     6601 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     6661 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     6721 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     6781 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     6841 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     6901 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     6961 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     7021 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     7081 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     7141 ttgaaaaaca cgataatacc tccggaatga ttgaacaaga tggattgcac gcaggttctc
     7201 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     7261 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     7321 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggccg
     7381 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     7441 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     7501 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     7561 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     7621 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     7681 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     7741 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     7801 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7861 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7921 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agtcgacaat caacctctgg
     7981 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     8041 gtggatacgc tgctttaatg cctttgtatc atgcgttaac taaacttgtt tattgcagct
     8101 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8161 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggaattga
     8221 ctcaaatgat gtcaattagt ctatcagaag ctcatctggt ctcccttccg ggggacaaga
     8281 catccctgtt taatatttaa acagcagtgt tcccaaactg ggttcttata tcccttgctc
     8341 tggtcaacca ggttgcaggg tttcctgtcc tcacaggaac gaagtcccta aagaaacagt
     8401 ggcagccagg tttagccccg gaattgactg gattcctttt ttagggccca ttggtatggc
     8461 tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag
     8521 gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg
     8581 ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag
     8641 cgggggaggg acgtaattac atccctgggg gctttggggg ggggctgtcc ctgatatcta
     8701 taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata
     8761 attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact
     8821 cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg
     8881 ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag
     8941 aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg
     9001 gttaatctag ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc
     9061 aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga
     9121 agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg
     9181 cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt
     9241 cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt
     9301 cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg
     9361 tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc
     9421 tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcttg
     9481 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9541 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9601 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9661 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9721 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9781 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9841 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9901 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9961 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10021 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10081 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10141 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10201 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10261 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10321 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10381 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10441 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10501 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10561 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10621 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10681 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10741 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10801 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10861 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10921 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10981 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    11041 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    11101 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    11161 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    11221 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11281 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    11341 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11401 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11461 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11521 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11581 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11641 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
    11701 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
    11761 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
    11821 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
    11881 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
    11941 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
    12001 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
    12061 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
    12121 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
    12181 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    12241 gtaaaacgac ggccagtgag cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg
    12301 ggcagactcg cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag atgctcgaca
    12361 cgctgcagaa cacgcagcta gattaaccct agaaagataa tcatattgtg acgtacgtta
    12421 aagataatca tgtgtaaaat tgacgcatgt gttttatcgg tctgtatatc gaggtttatt
    12481 tattaatttg aatagatatt aagttttatt atatttacac ttacatacta ataataaatt
    12541 caacaaacaa tttatttatg tttatttatt tattaaaaaa aacaaaaact caaaatttct
    12601 tctataaagt aacaaaactt ttatgaggga cagccccccc ccaaagcccc cagggatgta
    12661 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
    12721 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
    12781 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
    12841 ggggatacgg ggaaaa
//
