LOCUS       sequence-357920-       12901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Nav1.6 tagged with 3xV5 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Liu H, Wang HG, Pitt GS, Liu ZJ
  TITLE     Direct Observation of Compartment-Specific Localization and Dynamics
            of Voltage-Gated Sodium Channels.
  JOURNAL   J Neurosci. 2022 Jun 6;42(28):5482-98. doi: 
            10.1523/JNEUROSCI.0086-22.2022.
  PUBMED    35672149
REFERENCE   2  (bases 1 to 12901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0086-22"; journalName: "J Neurosci"; date: 
            "2022-06-6- 6"; volume: "42"; issue: "28"; pages: "5482-98"
COMMENT     Sequence Label: piggybac-Syn-mScn8a-3xV5
FEATURES             Location/Qualifiers
     source          1..12901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6460..6501
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6517..6558
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6574..6615
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6631..7203
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6798..6815)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7025..7044
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7212..8006
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7266..7285)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7876..7895
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8066..8086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    8128..8249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8165..8184)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8219..8238
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9012..9046
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9537..9553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9537..9553)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9550..9572)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9561..9577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9585..9591,9592..9609,9610..9615))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9630..9651
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9768..9785)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9939..10527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10019..10038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10698..11489,11490..11558))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11321..11340
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11559..11663)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11689..12144)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11826..11847)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12038..12057
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12271..12293
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12285..12302
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12286..12302
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   12432..12494
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ggcctccacg gccaattcca tatgtgacga ccgaccccga cccactggac aagcacccaa
       61 cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac aggatgcggc
      121 gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc ccgcctggcg
      181 gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc
      241 aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca gccggaccgc
      301 accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg
      361 actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc
      421 agctgtgctc ctgggcaccg cgcagtccgc ccccgcggct cctggccaga ccacccctag
      481 gaccccctgc cccaagtcgc agccacgcgt atggcagcgc ggctgctcgc accaccaggc
      541 cccgacagtt tcaagccttt cacccccgag tcgctggcaa acatcgagag gcgcatcgcc
      601 gagagcaagc tcaagaaacc acccaaggcg gatggcagcc accgggagga cgatgaagac
      661 agcaagccca agccaaacag tgacctggag gcggggaaga gtttgccttt catctacggg
      721 gacatcccgc aaggcctggt tgcggttccc ctggaggact ttgacccgta ctatttgacg
      781 cagaaaactt ttgtagtatt aaacagaggg aaaactctct tcagatttag cgccactcct
      841 gccttgtaca ttctaagtcc ttttaacctg ataagaagaa tagctattaa aattttgata
      901 cattcagtct tcagcatgat catcatgtgc accatcctga ccaactgtgt attcatgact
      961 tttagtaacc ctcccgaatg gtccaagaat gtggagtaca cattcacagg gatttacaca
     1021 tttgaatcac tggtgaaaat catcgccaga ggtttctgca tagacggctt caccttctta
     1081 cgagacccgt ggaactggtt agacttcagt gtcatcatga tggcgtatat aacagaattc
     1141 gtaaacctag gcaatgtttc agctctacgc actttcaggg tactgagggc tttgaaaact
     1201 atttcggtaa tcccaggcct gaagacaatc gtgggcgccc tcatccagtc tgtgaagaag
     1261 ctgtcagacg tgatgatcct gacggtgttc tgcctgagtg tcttcgccct gattggcctg
     1321 cagctcttca tggggaacct tcgaaacaag tgtgtcgtgt ggcccataaa cttcaacgag
     1381 agctatctgg agaacggcac cagaggcttc gactgggagg agtatatcaa caataaaaca
     1441 aacttttaca tggtccctgg catgctagaa cccttgctct gcgggaacag ctctgacgcc
     1501 gggcaatgcc cagaggggtt ccagtgcatg aaagcaggaa gaaatcccaa ctacggctac
     1561 accagcttcg acaccttcag ctgggccttc ctggcactgt tccgcctcat gactcaggac
     1621 tactgggaga acttatacca gctgacctta cgagcggctg ggaaaacgta catgatcttc
     1681 tttgtcttgg tgatcttcgt gggttctttc tatttggtga acttgatcct ggctgtggtg
     1741 gccatggctt acgaagagca gaaccaggca acactggagg aggcagagca gaaagaggca
     1801 gagttcaagg cgatgctcga gcagctcaag aagcagcagg aggaggcgca ggctgctgca
     1861 atggccactt cggcgggcac cgtctcagaa gatgccattg aagaagaagg ggaagatggg
     1921 gtaggctctc cgaggagctc atctgagctg tctaaactca gttccaagag cgcgaaggag
     1981 cgtcggaacc gacggaagaa gaggaagcag aaggagctct ctgaagggga ggagaagggg
     2041 gaccccgaga aggtgtttaa gtcagagtcg gaagatggca tgagaaggaa ggccttccgg
     2101 ctgccagaca ataggatagg gaggaaattt tccatcatga atcagtcgct gctcagcatc
     2161 ccaggctcgc ccttcctttc ccgacacaac agcaaaagca gcatcttcag ctttcggggt
     2221 cctggacggt tccgggaccc cggttcggag aacgagttcg cagacgacga gcacagcaca
     2281 gtggaggaaa gtgagggccg gcgcgactcg ctcttcatcc cgatccgcgc ccgcgagcgc
     2341 cgcagcagct actctggata ctcaggatac agtcagtgca gccgctcgtc gcgcatcttc
     2401 cccagcctgc ggcgcagcgt gaaacgcaac agcaccgtgg actgcaatgg cgtggtgtcg
     2461 ctcatcgggc ctggctcgca catcgggcgg ctcctgcctg aggcaacaac tgaggtggag
     2521 attaagaaga aaggccctgg atcgctctta gtctccatgg aacaactcgc ctcctacgga
     2581 cggaaggaca gaatcaacag tataatgagc gttgtcacaa acacactggt ggaagagctg
     2641 gaagagtctc agagaaagtg cccaccatgc tggtataagt ttgccaatac tttcctcatc
     2701 tgggagtgtc acccctactg gataaaactg aaggagattg tgaacctgat cgtcatggac
     2761 cctttcgtag acttagccat aaccatctgc attgtcctga acacactatt tatggcaatg
     2821 gagcaccatc ccatgacgcc acaattcgaa catgtcctgg ccgtagggaa cctggtgttc
     2881 accggtatct tcacggcgga aatgttcctg aagctcatag ccatggatcc ctactattac
     2941 ttccaagaag gctggaacat ttttgacgga tttatcgtct ccctcagttt aatggagctg
     3001 ggccttgcag acgtggaggg gctctcagtg ctgcgatctt tccgattgct ccgagtcttc
     3061 aaattggcca agtcctggcc caccctgaac atgctgatca agatcattgg gaactccgtt
     3121 ggcgccctgg gcaacctgac cctggtgctg gccatcattg tcttcatctt tgccgtggtg
     3181 gggatgcagc tctttggaaa gagctacaag gagtgcgtct gtaagatcag ccaggagtgc
     3241 aagctcccgc gctggcacat gaacgacttc ttccactcct tcctcatcgt cttccgagtg
     3301 ctgtgtgggg agtggatcga gaccatgtgg gattgcatgg aagtggccgg ccaggccatg
     3361 tgccttatcg tgttcatgat ggtcatggtg attgggaacc tggtggtgct gaacttattt
     3421 ctggccttgc ttctgagctc cttcagcgca gacaatctgg cggccacgga cgacgacggg
     3481 gaaatgaaca acctgcagat atcggtgatc cggatcaaga agggcgtggc ctgggccaaa
     3541 gtgaaggtgc atgccttcat gcaggcacac ttcaagcaac gggaggctga tgaagtgaaa
     3601 cctttagacg agctgtatga gaagaaggcc aactgcatcg ccaaccacac cggcgtggac
     3661 attcacagga acggcgactt ccagaaaaat ggaaatggca ccactagtgg catcggcagc
     3721 agcgtggaga agtacatcat cgacgaggac cacatgtcct tcattaacaa cccaaacctg
     3781 accgtgcggg tgcccattgc tgtgggcgag tctgatttcg agaacctcaa cacagaggat
     3841 gttagcagcg agtcagaccc cgaaggcagc aaagacaaac tggacgatac cagctcctca
     3901 gaagggagta ccatcgacat caagcctgag gtggaagaag tcccagtgga gcagcctgag
     3961 gaatacttgg atccagacgc ctgcttcacg gagggttgtg tccagaggtt caagtgctgc
     4021 caggtcaaca tcgaggaagg actaggcaag tcgtggtgga tcctgcggaa aacctgcttc
     4081 ctcattgtgg agcacaattg gtttgagacc ttcatcattt tcatgattct cctcagcagt
     4141 ggagccctgg ccttcgagga catctacatt gagcagagga agaccatccg taccatcctg
     4201 gagtatgcgg acaaggtctt cacctacatc ttcatcctgg agatgttgct caaatggaca
     4261 gcctatggct tcgtcaagtt cttcaccaat gcctggtgct ggttggactt cctcattgtg
     4321 gctgtctctt tagtcagcct tatagctaat gccctgggct actcggaact aggtgccata
     4381 aagtccctta ggaccctaag agctttgaga cccttaagag ccttatcacg atttgaaggg
     4441 atgagggtgg tggtgaacgc cttggtgggc gccatcccct ctattatgaa cgtgctgctg
     4501 gtgtgtctca tcttctggct gattttcagc atcatgggcg ttaacctgtt tgctgggaaa
     4561 taccactact gcttcaacga gacttcagaa atccggttcg aaatcgacga ggtgaacaat
     4621 aaaaccgact gtgagaagct catggagggc aacaacacag agatccgatg gaagaacgtc
     4681 aagatcaact tcgacaacgt cggggcaggc tacctggccc ttcttcaagt ggcaaccttc
     4741 aaaggctgga tggacatcat gtatgcagct gtagattcta gaaagccgga cgagcagcct
     4801 gattatgagg gcaacatcta catgtacatc tacttcgtca tcttcatcat cttcggctcc
     4861 ttcttcaccc tcaacctgtt catcggtgtc atcatcgaca atttcaatca acagaagaaa
     4921 aagtttggag gtcaggacat cttcatgaca gaggaacaga agaagtacta caacgccatg
     4981 aagaagctag gctccaagaa gccacagaag cccatccccc gacctttgaa caaaatccaa
     5041 gggattgtct ttgatttcgt cacgcaacaa gccttcgaca tcgtgatcat gatgcttatc
     5101 tgcctcaaca tggtgaccat gatggtggag acagacacac agagcaagca gatggagaac
     5161 attctctact ggattaatct ggtcttcgtc atcttcttca cctgcgagtg tgtgctcaaa
     5221 atgtttgcct tgagacacta ctatttcacc attggctgga acatctttga ctttgtggtg
     5281 gtcattctct ccattgtggg aatgttcctg gctgatatca ttgagaagta cttcgtctcc
     5341 ccgaccctat tccgcgtcat ccgattggcc cgcatcgggc gcatcttgcg tctgatcaag
     5401 ggcgccaaag ggatccgcac cctgctcttt gccttaatga tgtcgctgcc cgccctgttc
     5461 aacatcggcc tcctgctctt cctggtcatg ttcatcttct ccatctttgg gatgtccaac
     5521 ttcgcgtacg tgaagcacga ggccggcatc gatgacatgt tcaacttcga gacgtttggc
     5581 aacagcatga tctgcctgtt ccagatcacg acctctgctg gttgggatgg cttactgctg
     5641 ccaatcctga accgcccccc tgactgcagc ttggacaagg agcacccagg aagtggcttc
     5701 aaaggggact gcgggaaccc ctccgtgggc atcttcttct tcgtcagcta catcatcatc
     5761 tcctttctga tcgtggtaaa catgtacatt gccatcatct tggagaactt cagcgtagcc
     5821 acagaggaaa gcgccgatcc tctgagcgag gacgacttcg agactttcta tgagatctgg
     5881 gagaagtttg atcctgatgc cacccagttc atcgagtact gtaagctggc cgactttgcc
     5941 gacgccctgg agcatccgct ccgagtaccc aagcccaaca ccatcgagct catcgccatg
     6001 gacctgccca tggtgagcgg agatcgaatc cactgcttgg acatcctttt cgccttcacc
     6061 aagcgagtcc tgggagacag tggggagttg gacatcctgc ggcagcagat ggaggagcgg
     6121 ttcgtggcta gcaatccttc caaagtgtct tacgagccta tcacgaccac tctgcggcgc
     6181 aagcaggaag aggtgtctgc agtggtcttg cagcgtgctt acaggggaca cctggctagg
     6241 cggggcttca tctgcagaaa gatcacttcc aacaagctgg agaatggagg cacccaccga
     6301 gagaagaagg agagcacccc gtccacagcc tccctcccct cttacgacag cgtgacaaag
     6361 cccgacaagg agaagcagca gcgggcagag gaaggcagga gggaaagagc caagaggcaa
     6421 aaagaagtca gggagtccaa gtgcgggtct ggatcgggtg ggaagcctat acccaatcct
     6481 ctcctggggt tggacagcac cggcagtggt agcggtggca agcccatccc caaccccctg
     6541 ctgggcctgg actccaccgg ctccggcagt ggtggaaaac ctattcccaa cccactgctg
     6601 ggactggatt ctacatagta agcggccgcg cccctctccc tccccccccc ctaacgttac
     6661 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     6721 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6781 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     6841 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6901 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6961 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     7021 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     7081 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     7141 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata
     7201 atacctccgg aatgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     7261 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     7321 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     7381 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccgcgacg ggcgttcctt
     7441 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     7501 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     7561 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     7621 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     7681 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     7741 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     7801 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     7861 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     7921 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     7981 atcgccttct tgacgagttc ttctgagtcg acaatcaacc tctggattac aaaatttgtg
     8041 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8101 taatgccttt gtatcatgcg ttaactaaac ttgtttattg cagcttataa tggttacaaa
     8161 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8221 ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa atgatgtcaa
     8281 ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc ctgtttaata
     8341 tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc aaccaggttg
     8401 cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag ccaggtttag
     8461 ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt ccccgtatcc
     8521 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
     8581 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
     8641 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
     8701 attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca agaaaatata
     8761 tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt
     8821 aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata
     8881 gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc
     8941 gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata
     9001 tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa tctagctgca
     9061 tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct
     9121 gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga
     9181 gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat
     9241 gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg
     9301 ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat
     9361 cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga
     9421 cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca
     9481 gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta atcatggtca
     9541 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9601 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9661 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9721 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9781 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9841 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9901 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9961 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    10021 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    10081 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    10141 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    10201 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10261 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10321 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10381 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10441 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10501 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10561 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10621 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10681 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10741 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10801 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10861 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10921 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10981 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    11041 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    11101 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    11161 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    11221 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11281 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11341 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11401 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11461 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11521 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11581 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11641 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
    11701 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
    11761 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
    11821 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
    11881 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
    11941 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
    12001 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
    12061 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
    12121 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
    12181 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    12241 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    12301 gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga ggacgggcag actcgcggtg
    12361 caaatgtgtt ttacagcgtg atggagcaga tgaagatgct cgacacgctg cagaacacgc
    12421 agctagatta accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgtgt
    12481 aaaattgacg catgtgtttt atcggtctgt atatcgaggt ttatttatta atttgaatag
    12541 atattaagtt ttattatatt tacacttaca tactaataat aaattcaaca aacaatttat
    12601 ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
    12661 aacttttatg agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc
    12721 cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat
    12781 ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt
    12841 tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa
    12901 a
//
