LOCUS       sequence-357927-       14242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian co-expression of human SEPT9_i3 fused to GFP10 and of 
            human SEPT7 fused to GFP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14242)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 14242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i3-14-GFP10
FEATURES             Location/Qualifiers
     source          1..14242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    22..103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1661..2048
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     protein_bind    1742..1760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1778..1796
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1813..1831
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1849..1867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1885..1903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1920..1938
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1956..1974
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(4067..4084)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4294..4313
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4475..5176
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             5459..5639
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7167..7186)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7400..7419)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7519..7541
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7579..7597)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7665..7769
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7770..7838,7839..8630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7988..8007)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8801..9389
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9290..9309
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9543..9560
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9630..9650)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9635..9964
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9815..9950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11098..11163
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11293..11313
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11738..11872
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     11829..11848
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             12040..12673
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12720..12845
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13338..13571
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13756..13800
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14093..14210
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttggctgata cgcgtacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
       61 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      181 ctacaaatgt ggtatggctg attatgatcc tctagattaa aagatcttcc ctttcttctt
      241 gttcttttcc aaggttcttg aagagttctg ttgttctaaa atacgttgtt gagcttccca
      301 gtttgctttc tcatcctcga actgacgacg tttttcctcc aattctttgt gctgtgcttc
      361 caaattcttt ttcatttgct catggcgccg ctggagctca gcttcagagt ccttcagttt
      421 ttgaactttt tctttgacct tcatctcaaa cacctgctcc atctccatct ccatcttctt
      481 cattttagct acatgctccc ttctttcttc ttccatttgt gccagagggc tcttagtcag
      541 ctgcccttta ttcttgttgt tatcaactcc attataagtc acagctgcaa gttttctgct
      601 tctgtagttc tcatagtgga cattattagt aacatctttc aagtcctgca tgtgtgttct
      661 tatcaacata tttcttagga ttgtaaaatc acaatgttca ccattttcaa cttcagcaac
      721 accccaagga tactgccttc ctctgaccct tttgccatta acttcaatga tagtattact
      781 acctaccaca gcaagaggta aacggtcctt tatcttttta acaagtttat tttcttcttc
      841 atcatctgtt tctggaaatt cgtatatttt aattttatgt tcttggattt ctttcattat
      901 ctgtttttta aactgttggc attcctctgg tgtgagtgtg tctgctttgg caataagtgg
      961 gatgatattc actttttcat gcaaacgctt cataaactca atatccaatg gtttaagtcc
     1021 atgtcctgaa ggagcaatga agtataaaca acactgcacc ctgttatcag gcatctgacg
     1081 tctgttcact cgtgattctg catttaggta gtcctcaaat ttactatcaa tgtagtcgat
     1141 aacaggctgc cagcaattac tattatccac tgcatctcca aatcctgggg tatcaactat
     1201 tgtgagcagc aactgaacac caccttcttt gattaaaact ttggattgtt ccacctgtac
     1261 agtcttttta attctatgag aaggacctgg atactctgga gaatacaaat ctgtgaggaa
     1321 taatgagttg attaatgtcg actttcccaa tccagattca cccactacca taagcgtgaa
     1381 ttcaaaacct ctcttcaccg attttctgta tacttgattt gggagattgg caaatcccac
     1441 atagccttca aggttcttct gttgagctac catggtgctg ctgttgacgc tcctctcctc
     1501 agcagcagcg gatctcgcac tgaccgacat tccggaggac ccaccacctc cagagccacc
     1561 gccaccatcg atggaggcgt cggtgatgcc ggcggcggtc acgtactcca gcagcaccat
     1621 gtggtcgcgc ttctccatca tatgaccggt gaattctcca ggcgatctga cggttcacta
     1681 aacgagctct gcttatatag gcctcccacc gtacacgcca cctcgacata ctcgagttta
     1741 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga
     1801 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     1861 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
     1921 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     1981 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2041 agatcgcctg gagatttcga gctcggtacc gcggatggag agggaccgga tctcagcctt
     2101 gaaaagatct tttgaggtcg aggaggtcga gacacccaac tccaccccac cccggagggt
     2161 ccagactccc ctactccgag ccactgtggc cagctccacc cagaaattcc aggacctggg
     2221 cgtgaagaac tcagaaccct cggcccgcca tgtggactcc ctaagccaac gctcccccaa
     2281 ggcgtccctg cggagggtgg agctctcggg ccccaaggcg gccgagccgg tgtcccggcg
     2341 cactgagctg tccattgaca tctcgtccaa gcaggtggag aacgccgggg ccatcggccc
     2401 gtcccggttc gggctcaaga gggccgaggt gttgggccac aagacgccag aaccggcccc
     2461 tcggaggacg gagatcacca tcgtcaaacc ccaggagtca gcccaccgga ggatggagcc
     2521 ccctgcctcc aaggtccccg aggtgcccac tgcccctgcc accgacgcag cccccaagag
     2581 ggtggagatc cagatgccca agcctgctga ggcgcccacc gcccccagcc cagcccagac
     2641 cttggaaaat tcagagcctg cccctgtgtc tcagctgcag agcaggctgg agcccaagcc
     2701 ccagccccct gtggctgagg ctacaccccg gagccaggag gccactgagg cggctcccag
     2761 ctgcgttggc gacatggccg acacccccag agatgccggg ctcaagcagg cgcctgcatc
     2821 acggaacgag aaggccccgg tggacttcgg ctacgtgggg attgactcca tcctggagca
     2881 gatgcgccgg aaggccatga agcagggctt cgagttcaac atcatggtgg tcgggcagag
     2941 cggcttgggt aaatccacct taatcaacac cctcttcaaa tccaaaatca gccggaagtc
     3001 ggtgcagccc acctcagagg agcgcatccc caagaccatc gagatcaagt ccatcacgca
     3061 cgatattgag gagaaaggcg tccggatgaa gctgacagtg attgacacac cagggttcgg
     3121 ggaccacatc aacaacgaga actgctggca gcccatcatg aagttcatca atgaccagta
     3181 cgagaaatac ctgcaggagg aggtcaacat caaccgcaag aagcgcatcc cggacacccg
     3241 cgtccactgc tgcctctact tcatccccgc caccggccac tccctcaggc ccctggacat
     3301 cgagtttatg aaacgcctga gcaaggtggt caacatcgtc cctgtcatcg ccaaggcgga
     3361 cacactcacc ctggaggaga gggtccactt caaacagcgg atcaccgcag acctgctgtc
     3421 caacggcatc gacgtgtacc cccagaagga atttgatgag gactcggagg accggctggt
     3481 gaacgagaag ttccgggaga tgatcccatt tgctgtggtg ggcagtgacc acgagtacca
     3541 ggtcaacggc aagaggatcc ttgggaggaa gaccaagtgg ggtaccatcg aagttgaaaa
     3601 caccacacac tgtgagtttg cctacctgcg ggaccttctc atcaggacgc acatgcagaa
     3661 catcaaggac atcaccagca gcatccactt cgaggcgtac cgtgtgaagc gcctcaacga
     3721 gggcagcagc gccatggcca acggcgtgga ggagaaggag ccagaagccc cagagatgat
     3781 cgatggtggc ggtggctctg gaggtggtgg gtcctccgga atgggcgacc tgcccgacga
     3841 ccactacctg tccacccaga ccatcctgag caaggacctg aactaagcta gctaatagtt
     3901 ccgcccctct ccctcccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     3961 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     4021 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     4081 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     4141 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     4201 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     4261 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     4321 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     4381 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     4441 gacgtggttt tcctttgaaa aacacgatgc aagcatgagc gagctgatta aggagaacat
     4501 gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga
     4561 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
     4621 ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt
     4681 catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac
     4741 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
     4801 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa
     4861 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc
     4921 cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca
     4981 tctgatcgca aacatcaaga ccacatatag atccaagaaa cccgctaaga acctcaagat
     5041 gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac
     5101 ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg
     5161 gcacaagctt aattaaggat ccagcggccg ctcgagacct agaaaaacat ggagcaatca
     5221 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     5281 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     5341 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5401 aacgaagaca aaatcgtcga gagatgctgc atataagcag ctgctttttg cttgtactgg
     5461 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5521 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5581 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5641 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     5701 gagtgagagg ccttgacatt ataatagatt tagcaggaat tgaactagga gtggagcaca
     5761 caggcaaagc tgcagaagta cttggaagaa gccaccagag atactcacga ttctgcacat
     5821 acctggctaa tcccagatcc taaggattac attaagttta ctaacattta tataatgatt
     5881 tatagtttaa agtataaact tatctaattt actattctga cagatattaa ttaatcctca
     5941 aatatcataa gagatgatta ctattatccc catttaacac aagaggaaac tgagagggaa
     6001 agatgttgaa gtaattttcc cacaattaca gcatccgtta gttacgactc tatgatcttc
     6061 tgacacaaat tccatttact cctcacccta tgactcagtc gaatatatca aagttatgga
     6121 cattatgcta agtaacaaat taccctttta tatagtaaat actgagtaga ttgagagaag
     6181 aaattgtttg caaacctgaa tagcttcaag aagaagagaa gtgaggataa gaataacagt
     6241 tgtcatttaa caagttttaa caagtaactt ggttagaaag ggattcaaat gcataaagca
     6301 agggataaat ttttctggca acaagactat acaatataac cttaaatatg acttcaaata
     6361 attgttggaa cttgataaaa ctaattaaat attattgaag attatcaata ttataaatgt
     6421 aatttacttt taaaaaggga acatagaaat gtgtatcatt agagtagaaa acaatcctta
     6481 ttatcacaat ttgtcaaaac aagtttgtta ttaacacaag tagaatactg cattcaatta
     6541 agttgactgc agattttgtg ttttgttaaa attagaaaga gataacaaca atttgaatta
     6601 ttgaaagtaa catgtaaata gttctacata cgttcttttg acatcttgtt caatcattga
     6661 tcgaagttct ttatcttgga agaatttgtt ccaaagactc tgaaataagg aaaacaatct
     6721 attatatagt ctcacacctt tgttttactt ttagtgattt caatttaata atgtaaatgg
     6781 ttaaaattta ttcttctctg agatcatttc acattgcaga tagaaaacct gagactgggg
     6841 taatttttat taaaatctaa tttaatctca gaaacacatc tttattctaa catcaatttt
     6901 tccagtttga tattatcata taaagtcagc cttcctcatc tgcaggttcc acaacaaaaa
     6961 tccaaccaac tgtggatcaa aaatattggg aaaaaattaa aaatagcaat acaacaataa
     7021 aaaaatacaa atcagaaaaa cagcacagta taacaacttt atttagcatt tacaatctat
     7081 taggtattat aagtaatcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
     7141 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     7201 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     7261 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     7321 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     7381 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     7441 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     7501 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     7561 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     7621 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7681 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7741 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7801 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7861 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7921 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7981 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8041 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8101 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8161 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8221 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8281 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8341 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8401 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8461 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8521 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8581 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8641 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8701 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8761 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8821 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8881 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8941 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9001 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9061 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9121 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9181 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9241 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9301 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9361 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9421 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9481 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9541 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9601 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     9661 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     9721 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     9781 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9841 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9901 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9961 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
    10021 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
    10081 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
    10141 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
    10201 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
    10261 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
    10321 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
    10381 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
    10441 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
    10501 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
    10561 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
    10621 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
    10681 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
    10741 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
    10801 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
    10861 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
    10921 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
    10981 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
    11041 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
    11101 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
    11161 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
    11221 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
    11281 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
    11341 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
    11401 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
    11461 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
    11521 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
    11581 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
    11641 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
    11701 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    11761 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    11821 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    11881 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    11941 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    12001 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
    12061 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
    12121 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
    12181 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
    12241 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
    12301 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
    12361 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
    12421 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
    12481 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    12541 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    12601 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    12661 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    12721 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    12781 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    12841 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    12901 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    12961 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    13021 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    13081 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    13141 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
    13201 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    13261 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    13321 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    13381 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    13441 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    13501 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    13561 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    13621 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    13681 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    13741 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    13801 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    13861 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    13921 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    13981 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    14041 aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa
    14101 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    14161 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
    14221 acagggacag cagagatcca ct
//
