LOCUS       sequence-357931-       14031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA,
            and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI 
            sites for spacer cloning. pBBR1 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14031)
  AUTHORS   Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg SH
  TITLE     CRISPR RNA-guided integrases for high-efficiency, multiplexed 
            bacterial genome engineering.
  JOURNAL   Nat Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y.
  PUBMED    33230293
REFERENCE   2  (bases 1 to 14031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y."
COMMENT     Sequence Label: pSL1142 (pSPIN, pBBR1 backbone)
FEATURES             Location/Qualifiers
     source          1..14031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(317..1111)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(428..447)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1038..1057
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      2591..3362
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3363..4025
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     4308..4330
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4322..4339
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4323..4339
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4539..5198)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5113..5132
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(5353..5400)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     5396..5414
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(6849..6860)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(13842..13876)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     complement(13887..13904)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtttgcgtat tgggcgcatg cataaaaact gttgtaattc attaagcatt ctgccgacat
       61 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
      121 cttgcgtata atatttgccc atgggggtgg gcgaagaact ccagcatgag atccccgcgc
      181 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
      241 gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
      301 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
      361 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
      421 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
      481 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
      541 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
      601 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
      661 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
      721 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
      781 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
      841 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
      901 ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg
      961 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     1021 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     1081 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     1141 cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     1201 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     1261 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
     1321 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atcccaggtg
     1381 gcacttttcg gggaaatgtg cgcgcccgcg ttcctgctgg cgctgggcct gtttctggcg
     1441 ctggacttcc cgctgttccg tcagcagctt ttcgcccacg gccttgatga tcgcggcggc
     1501 cttggcctgc atatcccgat tcaacggccc cagggcgtcc agaacgggct tcaggcgctc
     1561 ccgaaggtca cgggccgtct cttgggcttg atcggccttc ttgcgcatct cacgcgctcc
     1621 tgcggcggcc tgtagggcag gctcataccc ctgccgaacc gcttttgtca gccggtcggc
     1681 cacggcttcc ggcgtctcaa cgcgctttga gattcccagc ttttcggcca atccctgcgg
     1741 tgcataggcg cgtggctcga ccgcttgcgg gctgatggtg acgtggccca ctggtggccg
     1801 ctccagggcc tcgtagaacg cctgaatgcg cgtgtgacgt gccttgctgc cctcgatgcc
     1861 ccgttgcagc cctagatcgg ccacagcggc cgcaaacgtg gtctggtcgc gggtcatctg
     1921 cgctttgttg ccgatgaact ccttggccga cagcctgccg tcctgcgtca gcggcaccac
     1981 gaacgcggtc atgtgcgggc tggtttcgtc acggtggatg ctggccgtca cgatgcgatc
     2041 cgccccgtac ttgtccgcca gccacttgtg cgccttctcg aagaacgccg cctgctgttc
     2101 ttggctggcc gacttccacc attccgggct ggccgtcatg acgtactcga ccgccaacac
     2161 agcgtccttg cgccgcttct ctggcagcaa ctcgcgcagt cggcccatcg cttcatcggt
     2221 gctgctggcc gcccagtgct cgttctctgg cgtcctgctg gcgtcagcgt tgggcgtctc
     2281 gcgctcgcgg taggcgtgct tgagactggc cgccacgttg cccattttcg ccagcttctt
     2341 gcatcgcatg atcgcgtatg ccgccatgcc tgcccctccc ttttggtgtc caaccggctc
     2401 gacgggggca gcgcaaggcg gtgcctccgg cgggccactc aatgcttgag tatactcact
     2461 agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta cgggcttgct
     2521 ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga tatgtggacg
     2581 atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga
     2641 caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct
     2701 acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat
     2761 caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg
     2821 ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg
     2881 gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga agggcgaagc
     2941 ccgcccgcct gccccccgag cctcacggcg gcgagtgcgg gggttccaag ggggcagcgc
     3001 caccttgggc aaggccgaag gccgcgcagt cgatcaacaa gccccggagg ggccactttt
     3061 tgccggaggg ggagccgcgc cgaaggcgtg ggggaacccc gcaggggtgc ccttctttgg
     3121 gcaccaaaga actagatata gggcgaaatg cgaaagactt aaaaatcaac aacttaaaaa
     3181 aggggggtac gcaacagctc attgcggcac cccccgcaat agctcattgc gtaggttaaa
     3241 gaaaatctgt aattgactgc cacttttacg caacgcataa ttgttgtcgc gctgccgaaa
     3301 agttgcagct gattgcgcat ggtgccgcaa ccgtgcggca cccctaccgc atggagataa
     3361 gcatggccac gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg
     3421 tgcaaacgga acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg
     3481 cggcaatgct gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca
     3541 gccagaagac actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca
     3601 aggacttggt ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt
     3661 cggcctacgt ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt
     3721 cggtgttcag tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc
     3781 atggcgacct gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc
     3841 ccggcgagga gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct
     3901 tgaccgaaac ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc
     3961 cgtgttttct ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc
     4021 ggtagcactt gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc
     4081 cgcctcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
     4141 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca
     4201 ggctctggga ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct
     4261 gagcaaactg gccgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     4321 ttgtaaaacg acggccagtg aattcgagct cggtacctgt tgatgcaacc ataaagtgat
     4381 atttaataat tatttataat cagcaactta accacaaaac aaccatatat tgatatctca
     4441 caaaacaacc ataagttgat atttttgtga atcgagtatt tcagcaaaac tactgcagta
     4501 aggactctag aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca
     4561 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     4621 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     4681 atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     4741 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     4801 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     4861 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     4921 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
     4981 aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     5041 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     5101 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     5161 atatcaacgg tggtatatcc agtgattttt ttctccatgg atccccggaa atgacctcga
     5221 ggaaaatgtc gtaaacttac tgaagtttag accatgaaga ggcaatatca atttatgggt
     5281 gtgataatta tcaatttatg ggtgtaatta tcattttatg gttgtatcaa caacaaatgc
     5341 ctgaggtttc agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag
     5401 ttattgctca gcggtggcag cagcgcagca gcctaggtta ttcgaaaaag tttttatttc
     5461 tacctggttt actcactctc ggggttgaat gtctcaaata gtcctcccaa ccaatatctt
     5521 ttggtggcgg tcgtaacata tcttctgtta cctcaacacc ctcctcaaaa ggatttttcc
     5581 atgagttggg tttatcgccc gatgtcaact tcgatgcaaa aacgaaatct tcttcggtta
     5641 tcgtctcatg ctgattatca attgcttcga tagatgcttg ataaataagg tttctcaacg
     5701 aacgcatgtt tccctgagag aatgcataca attttttctg caaactttcg ttggctaagc
     5761 cagcctgttt ttcaaaaggt agggctttat caaggtattc caaaaaagtt ttaaatacac
     5821 ctctacctcc ctggtagcta aaggggcgca gttcaacctg aatggaaaat cgcccgtgaa
     5881 gttgcgagtt tgcttgcagt acaacttttg agtatggcat accaaatata acaattggac
     5941 atttcgtttt gttaagtatc atttttagcc aattacctac ttgggtaaga acccgatttg
     6001 acctttcttc caccaaatgt tggaactcat cgataataat cagcttcacg ccgaccgcag
     6061 ggattaattc agtcagtctt ttcgtcaatc tagctaagtc agtttcataa agtgctagcg
     6121 ggtcacccat ttcaaccagc aattcccttg ctgcatctac tggcttcgca ttgtctggca
     6181 actcaatatg caaaaccggt attatatcgc ccccggcttc ggactctctt ctgttttgat
     6241 ttaagtattt cttgatgacg gtcgttttac ctacacccga agcaccatag accatcatgc
     6301 atgtaggctc agactctagg tctgatagat ctctacatct atccatgtaa ctcaagattt
     6361 tcctaacgct cggtgtggat acaaatgccc ttttagctct tgatatacga gcctcacgcg
     6421 tttcactcat ttgacgattt ctcagacata atcaatatcc caatcgtcat tatctgcact
     6481 aacgatttca tcttcatgtt tttgtatcga ggccgggttc gcattactga ttgacttagc
     6541 ccttgcgcta ttctcttgat gcctagcgcc acgcctccga gctctgattt tgttagtctt
     6601 aacaattgaa cgatctgcga tttcttcaat ttttatttct gcatcaattt ccttgtcctc
     6661 atcatattct gctgaattta gttctgcttg atatttcatg ttatatttat gttgccaaag
     6721 tgatactcta cttgcatatt cgtagtcaat cgcattaact gtaaagtact cattcatatc
     6781 ctcatccaac acccaaataa ctgccataca ctcagggtta tacttgaact gaactttatg
     6841 gtttcctttc ttccctctat attcagctaa acggtcattg ctataactca gttctttgta
     6901 gacagttatc cctactttag taagttgttt gtaatcaaca atagcaaatt taaagtctaa
     6961 ttcgtcttta gaaccagaga actcctctgg ttcccattct tgacaaccct ttttccacgc
     7021 aacattagga caattagtgc ctctctgatt gggtttttta tgataaatat ccaccaacca
     7081 aattaagtaa atttctctaa tctcattgag tgtaagggta gcttctccca cagagtcgta
     7141 cccttctctt tgaaggtact ggctgaagga tttcccaggt aaatcgtcta acagagaagt
     7201 attaatagtc ccgtagttac gttcaacatg aggtttgttg tcgggcgtct caactttatt
     7261 ttgatgcaca ttgatcaata gtgattcaca tgcttgatca aatgctttcg acaaaaactc
     7321 tttaccatta tcagttacga gtaggtctgg gatgccataa catagccatt cattctcgat
     7381 cgattcatac gaggagatta agtcatcttt gcgttgtatt gcattcttaa gtgcaaggga
     7441 aaccgacaca tagctaggag gctcgaaacc taaataaaaa ccgattacag ctttactgta
     7501 acaatcaacc aattgagtaa gccaaggtcg gcccaatggg attcgatact cttcgtgtac
     7561 tgcaaaaagg tcaacgacag tgtgatctat ttcaaccctc tctagcacgc tagacgtgag
     7621 gatctttttt cccattcggc gaaactctct cttagctact ctctcccctt tccctgcggc
     7681 taataactca aatggggttt tcttttttac tcgctttctt acagattcgt acttaggata
     7741 cgtgtatttc gttccatgag ttagattgta ttgacgaact tttcgtcgaa cacgtttata
     7801 tgcagagcta acattgactt ttcgtccaga tataactctt tcaactgcct gttccataat
     7861 agaatcaaca actgttgaca cttttgtctc tctattacct ctatccttga tattaggtgc
     7921 taagctaatg gggttgtagt ctgattttcg aaaagcaagc caccatcgat atatcgttat
     7981 tgcactgggt atttttctac tatagttttg ctgtagctct acacgatgtg gttcaattga
     8041 tttggccaca atggtttcgc actttcgcgc taaaaagcga agaatgctta atcggaaatt
     8101 aatctctacc gcaattgttt cgggaaatgc tgatatatct tgatacgtaa cactttctag
     8161 acaattagcg ctagagacaa gctctatcga ttcaagcgta tcttgggaag agactgcttt
     8221 tctatggaaa cttgagaacc ctttcttagc cataactggc caactcaaat cgagattcaa
     8281 gagatagagg cttatccaaa cgtgtatcga gtaaacacct tgacagcaaa tcgcacaaaa
     8341 ttggtaaaac agttcggcct tttaaaccca actgttctcc caagcatctt gcagattggg
     8401 cgccattgta ctttattatt tttaagagag agttatgtac gttattcagc tcatctttcg
     8461 aagcataacg atgcatgcgc ttaagattat ttaatacaac cgtatcccta atatcactct
     8521 cttcaacaag ctccaattct agccctagtt cagttgctgc ttttactttc gcctcccatt
     8581 caacatcaaa atcaggtttg ttcttagcat aagctgactt tacttcatat agaacaaact
     8641 cgttggtatc aaattgaact agaaagtcag gaacatagga gtgagactga ccattgagat
     8701 aatataaaaa tcgaatcggt tgagcgcaaa agcgaacaat acttggctca aaatcaaagt
     8761 gatagcaggc atcacactct agagtagatt ctaccgtaat cctcttactc atctttacac
     8821 tgacataacg atgaatgttt tttccgcgag atttcgttag attgcgagcg ggagtatgga
     8881 atgcatactc taacgccgaa gtcgtaattg ctgagggcgt aggtaaactt gtcgccatgg
     8941 tatatctcct tattaaaggt cagattaagg gtacaggctg aaacgagttt tctgaatttg
     9001 ataagccata gctactaaaa atcgagttcc cctcacgggg ttgctccgag agcatcctga
     9061 tattgagtcg aaagttgcgg ttcgtctgtt tgctggactc cccaagtgag tggtagtgcg
     9121 ctattgcagt atgctctttt tgagcaaaag atgatgggtc aaattgctcg cctctagata
     9181 gagcacgttt ctccaggcgt ctgattttcc ttgccagccc tgctgctgtg agcttatcta
     9241 tagattgaca gcgtctaaag gaaacgtatg tacaatcttc tgggacgaga acagtgttgg
     9301 atatatgaaa gtattgaagt tgttccattt ggacgaaata gtgttgttta agtagtaagt
     9361 cgagttctat cttgttcttg ctgacaaatg aaatcttttt tccaaccgtt gcatcacacc
     9421 aaagcggaaa tgaaacgccg atatttcgtg tctcatactg atagttaaat ccatgcagaa
     9481 cgcggagaca ctttgcagcc agtgactcgt tgttgcacaa ctctggcaga aaggtgattg
     9541 tcttataata ccatttcaca gtcagtctcc tttatgctgg aacatcccac ccttaatcag
     9601 gttagccatt aaaaagtgca tgtcgttgat agtttcttga gcgggagttt tgttggcgct
     9661 caacacttca atatagtgct ctgcttgttg taatatcgag aaaaaatctt ttccggtaga
     9721 cggatgacgg tagcaagtga catcttcgcg atgaacccca aaccgtccga cccttagtgg
     9781 ctcagtggct tcaggatacc agtcgtcaat ggttgcaata gctgctcccg ttttaaaggc
     9841 ccctagtatt ggcgatcgtt caccatcaat agttgtactc tgaaaaactc gactgttttg
     9901 agttttagat ttacttttac tgccactttc tttttctgtg aaaacttggc ttggccgcac
     9961 catagcattc gttggcaagt gcaaggtggc cctgacttca aatatcgcca gcccatcagt
    10021 actagaaaat gcggttttga tcatttcaac tatagcagac caatttttat tatttttaaa
    10081 gtcttgccgt gaggtgtagt tagtacgaat atcttcaaat ccaacctttt ccccgttcca
    10141 aggccaaggt gtaagtacaa tgttccagca ataggcttta cgggtatttt tccacagcca
    10201 tttaccgttg caaatgttca tcaaatatcg ggttgctagc tcagtccagc cgattttcgt
    10261 ttcgtagagc tcgactaatt gcactagcgt ttgtttaact ttgcttgagt tacacttata
    10321 tggttgacgt agttcagaag aaaacgagac ggagaaactg cattcaatat gactcgcacc
    10381 gtatggaacg tggcagaaat caacttgatg agggttccct tgagctaacg cctcagcggt
    10441 ggcacttttt attggttgac ctgagacatc ataggctaat gatgccgctt ccatttgccc
    10501 gagcagagta cgagaattgt aggttaaagg tgtttttcta tcatcgggcc agacgacaaa
    10561 aaaacagaca tctgatgggt cgatagagcg ctcataggct agatttgtcg gtagtttcat
    10621 cgttgatact ccttggctgt accactaggt agttttgata gttctttagc gaccaaaaga
    10681 ttgtgttggg atcagtctct gagctaaagg tgattgagtt aatgagtcca atgatgcact
    10741 cggcgatagc gtgtttgtaa cctctgaggg agtttggttt atctttgggc tcttctaaca
    10801 agtggtaacc gacgcagctt gccatgagct gatggtcctc tgtcatggct gctaatagat
    10861 cacttagatt attgtttgac tgtgcgtaaa gtgataacca cctaccagct tctattggca
    10921 gagattcgat cgctgtttct aatgtcgaga atgagttgat atgtttaaag tcatcaatcg
    10981 caatttttgc tgaaccccga gccaatcgct ttggtagtga tgtaactaac gtatcttggt
    11041 caatatgttg agcaaagttg gtgttcaaaa taaggctaaa tacgacatca cactgccagt
    11101 catcccgggt cgcgggagcg gatatagtcc cgtcgccttt ttccacaaac tctgctgtca
    11161 aacctcgctt ctcgacatgt agttgatgga cgcatatcgc aaaggattca acacgaaacg
    11221 atgaggtggt gcgatttata ttccgttcaa atgcgtgaac gaagccaaag aaagctgtaa
    11281 gtgatggaat ccccaccgtt accggggagc ttaaagctgt tgcgccacaa accctgatat
    11341 tcggtaggac taaaaacgat ccattagtgt gttgctcacc attattgatt gaatttgata
    11401 actctcgttt caaaagctca gtaaagagct tggtcatcgc tggatcgtat gcaaaacgtt
    11461 ttgtggctcc taagtaagat aggtcatgat tcaaatgttg gataagtatg ttcgaatcaa
    11521 gggtatggtc ttgcattgca aaccaagatc tgaccatatt ttgaagctcg attttgtatt
    11581 ttctacggag tgcaatccga gagttttcag gtatgaggct tttgttgagg cgctggtact
    11641 gttttaatga ctgaacatgc tctgacgtca accaacttcg tttactgttt aatcggtgat
    11701 gaggggggct ggaaaactta gcgccagact ttaacattct aaatgcgccg ccacatgtca
    11761 tcgcagtgac ccccatgttg gtagttcggc taaaccgcgt tatcgcgcta tgccgatttt
    11821 catcttggag cctctgatga aagtggcttt gcatcgatag cgatgcaaca ggagagagtg
    11881 aaatgtagga tgtatcacta tcggggagag atatttgagt caggtaattt ggtgcgagtg
    11941 tgacattgat agtcgtgaga gtaatatcag ctagttgttt tgccattgct ttgcaagttt
    12001 tttgagaaca acctaacttt ttcaaagtat tccaaagtgg gtgatctgca tctttaagaa
    12061 gctcacccaa acatgagatc tcaccatccc aacaaaactc attggtgagg aatgcgcttt
    12121 tgttgacgta ttttgaatca tggctatatg accaatggta tggtgggatt tttgaggacg
    12181 agagtaggaa actcggtaat tcacccaggg cttgggttcg aatcacgcct tcagaacgta
    12241 tatccgggaa ttttgggtta tggctttgtc tgtaattaac gcaatttatg cagctagccc
    12301 accatttttc atctcgaagt ttttgcttgc atttggctcg atcgagcagg tctttctgat
    12361 catcagtctt atcggttaaa ttgactaata tcgtcagtgc gtcaagttca ttaccatcaa
    12421 ttgcaatatg cggtgtgagt ggacgaaatg ctctcttaag ttcagttgtt aagtcgtcag
    12481 gattggatgc gattagttct ttcagagttt gcatttacca cctcgacaca taaaacgaac
    12541 ggttaaactc atcgctttgg aactcagcta accaaagaca gaaaatatcg ccgaaatgaa
    12601 atagataatc ggtaacatca agagggctgt tgggcttgct ttttcgattt ggcttcaaga
    12661 tgcgttgttc aaccattgat gcaatctgat cgaggctaca atttaacatt acagtcgcct
    12721 ctaacgcgtt cattttgagg ttggcgatta atcccttgtc ttgccataaa cgattttcta
    12781 aatagcagag aagctcacca aggatgatat tgtgttgaag attccgctca ggtaaccgaa
    12841 ttgaaccgaa aaacaatcga ccaagaagat cttttagtcg taattcagac gtgctgacaa
    12901 cagcatgctc aaggttgaac tctacttcat cttcgattat cgagtggaat gaccttggcc
    12961 agtttgagaa aaattgaacg aacgaaaagt gatcgaactc gctatcttta atacccatcc
    13021 accaatgaac taaaccccat cggtagcttt taggaagatt tggcagaggt ttagattcat
    13081 ggccagcaag ccagtttgaa acagtacacg ctgcctcatg accgttctcc ctgctggtta
    13141 aggtgatagg ctccttgcat ttgcagcaaa tgccctttaa cccagatact cggtagtcaa
    13201 actccttacc acagctacaa gtggtaatta aaggtacgtt atggctgtgg cagtattcgt
    13261 acccctgaaa gtgccaaagg taggaggcgt agccattttc tcgcagacac aaaggacagc
    13321 aggggatgga gtgcgtccgt agtaaactgc gaggaaagac ttctgcacct ctaacaaccg
    13381 cgctagttga cggcgagtat ttcatgtttg ttctgttaat tgccaaccca agtagctctg
    13441 gcggttcatt aaatgtcaat tgtgcaagtt tcaggaatga cgcagttcgt gcgctggaac
    13501 tgtttttagc tgagtatggg tttatccgag ttatatcagt cggaaaggtt tgatagccat
    13561 tatggtcaat gtcttgaagg aatcgtttag tggcttctag gaagcgatgg acatcaccgt
    13621 agccattttt gttagccact cggataaaga aactttctaa actttcatcg ctgtaaggtt
    13681 taggtctttg caaaaacata tgtatatctc cttcgctcga attcggatcc gttatcagct
    13741 acctactcgg cagttcacga gaccacccta gctgacaaga cagaggtctc gttatcagct
    13801 acctactcgg cagttcaccc atggtatatc tccacgtcga cgctagtatt atacctagga
    13861 ctgagctagc tgtcaaagct tgtgtcctcg ctcactgact cgctgcgctc ggtcgttcgg
    13921 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac cagtcgggaa
    13981 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc g
//
