LOCUS       sequence-357932-        9122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector to knock in Halotag C-terminal to human CRY1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Gabriel CH, Del Olmo M, Zehtabian A, Jager M, Reischl S, van Dijk H,
            Ulbricht C, Rakhymzhan A, Korte T, Koller B, Grudziecki A, Maier B, 
            Herrmann A, Niesner R, Zemojtel T, Ewers H, Granada AE, Herzel H, 
            Kramer A
  TITLE     Live-cell imaging of circadian clock protein dynamics in 
            CRISPR-generated knock-in cells.
  JOURNAL   Nat Commun. 2021 Jun 18;12(1):3796. doi: 10.1038/s41467-021-24086-9.
  PUBMED    34145278
REFERENCE   2  (bases 1 to 9122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-18"; volume: "12(1):3796. doi"; pages: " 
            10.1038/s41467-021-24086-9"
COMMENT     Sequence Label: pDB-hCRY1-Halo-CD4-bla
FEATURES             Location/Qualifiers
     source          1..9122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    1060..1107
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1114..2001
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             2008..2025
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2035..2058
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2087..2120
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(2128..2161)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2244..2264)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2249..2579
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2430..2565
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2492..2511
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      2629..2638
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2635..2637,2638..2640,2641..3351)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2680..2701)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2941..2960)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3288..3309
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3367..3423
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3430..3825
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3837..3958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3874..3893)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3928..3947
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3986..4019
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(4020..4053)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5014..5217
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5168..5188
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5214..5238
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(5312..5386,5387..6583)
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(6901..6917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6901..6917)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6914..6936)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6925..6941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6949..6955,6956..6973,6974..6979))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6994..7015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7132..7149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7303..7891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7383..7402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8062..8853,8854..8922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8685..8704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8923..9027)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9095..9113
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatggg ccggccctta aggtttaaac tcatattttt ccataaactg gacacactga
      241 cagaaatgtt tccctaaaac atacttctct gttctagcca acctattctc ctataatagt
      301 gtttgttttt agaagggttg ataggattgc ttaggctagg taactgccac atagtctgtg
      361 tcttctgctt gtcgtatgat ttgaattgtt taactcatcg ggcaggccct tattccctta
      421 tcctcaagga aatatgtttg gaggagctct gaaggaaaag cgtgggaaaa gggacaagag
      481 tgagggagga atgtctatct cactcttgcc cctatgtttt accttcatgg tcaagtttca
      541 aattgtcatc tagtaaggaa atgtttttta gaaaatgatc atcaatgatt tttcctttga
      601 gctgtcaaca cttctgtgag cctaaattta agttttcctg gtttgagcat gtgatttttg
      661 gttcatagga agattgagat ttagtcttaa aagcatataa atgaccttaa gtacaagcgc
      721 taaaagttgg atttgtagac ttaataatac atacatcttg gatttgattt taagtaattt
      781 tactgtgttt taaatactaa caggttgctc tcaagggagt ggtattttac actatgctca
      841 tggcgacagt cagcaaactc acctgttgaa gcaaggtaag aatgaagcat tggagcatac
      901 tgttcttttt ccttttccta tcttaaacat acatttttta aatgtgcagg aagaagctcc
      961 atgggcactg gtctcagtgg tgggaaacgt cctagtcaag aagaagacac acagagtatt
     1021 ggtcctaaag tccagagaca gagcactaat agcggccgcg aagttcctat tccgaagttc
     1081 ctattctcta gaaagtatag gaacttcacc ggtgcagaaa tcggtactgg ctttccattc
     1141 gacccccatt atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc
     1201 gatggcaccc ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac
     1261 atcatcccgc atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc
     1321 aaatccgaca aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc
     1381 ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct
     1441 ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag
     1501 ttcatccgcc ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag
     1561 gccttccgca ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag
     1621 ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc
     1681 gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca
     1741 atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac
     1801 cagtcccctg tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc
     1861 gaagccgctc gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt
     1921 ctgaatctgc tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg
     1981 tcgacgctcg agatttccgg tactagtcac catcaccatc accatggtag cggtgactac
     2041 aaagacgatg acgacaaggc tagcaaagat ctgaagttcc tattccgaag ttcctattct
     2101 tcaaatagta taggaacttc agatctaata acttcgtata gcatacatta tacgaagtta
     2161 ttttgaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgttgagctg
     2221 atttaacaaa aatttaacgc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     2281 ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     2341 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2401 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2461 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     2521 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     2581 agctattgta tatccatttt cggatctgat cagcactgag caggtaccgc caccatggtg
     2641 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2701 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     2761 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2821 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     2881 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     2941 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3001 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3061 gagtacaacg ccatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     3121 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3181 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3241 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3301 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggaattcgga
     3361 agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
     3421 cctcacgtgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     3481 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     3541 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     3601 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     3661 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     3721 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     3781 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaactcga gaccccaact
     3841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3901 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3961 atgtctgtat agaagttcct attccgaagt tcctattctt caaatagtat aggaacttca
     4021 taacttcgta tagcatacat tatacgaagt tatttgatta attaagcgat cgcttcgaat
     4081 gcactaatta ggtaaatatt ttagagctgt atttcttgct ttagaagagt atataattaa
     4141 cataaattaa gataatttca aaaatggagc aaatctctat tttcaaacca gaaaatcttg
     4201 aggcattaat ttttaagcaa tttttacaaa ctcagttaat ttttggtcaa gagacatgca
     4261 tctgtactgg agaaattgtt gcaccagttt tatattcatc tgaaccaatg ctctttaaat
     4321 tagagatgtt tatgattttg tggtcaagtt ttttcttaga aaaagacaac ttttttattt
     4381 ccttactatg taactatgag tctaaaacaa ttaaagtggc ctgtttattt tagtgacatt
     4441 aatataatct ttttatgaac tttcccctaa atctttgcct cttaaatgtt gataaatttc
     4501 ttttatctgt tatgatgctt ctaaaatcta aattatttcc aatttgggaa tagttcaaaa
     4561 ttttttaaaa tgctggccct tattagaagt atcagaaagc cttgcctgca ttcaatttaa
     4621 ttggatttgg gatgtcattt tgtgatttaa attaatatga aaaatattta tacgttggat
     4681 ttgccagttt ttaaaaattt tctgtttctt cagtttctaa cactgtttac attttttatt
     4741 gcttagtttt tttatgtcaa cctaattaga ctataagtat cttgaagata aggtcaataa
     4801 acactcatca catttttgtc attgaattat ttgcaatcaa gctttaccta gttttttttt
     4861 cccccttaaa tcacagaaaa cattcaggag gaatactggt cgacaagctt gcatgcctgc
     4921 aggtcaattc cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     4981 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     5041 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     5101 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     5161 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     5221 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     5281 caccgggacc gatccagcct ggggatccac catgaaccgg ggagtccctt ttaggcactt
     5341 gcttctggtg ctgcaactgg cgctcctccc agcagccact cagggaaaga aagtggtgct
     5401 gggcaaaaaa ggggatacag tggaactgac ctgtacagct tcccagaaga agagcataca
     5461 attccactgg aaaaactcca accagataaa gattctggga aatcagggct ccttcttaac
     5521 taaaggtcca tccaagctga atgatcgcgc tgactcaaga agaagccttt gggaccaagg
     5581 aaacttcccc ctgatcatca agaatcttaa gatagaagac tcagatactt acatctgtga
     5641 agtggaggac cagaaggagg aggtgcaatt gctagtgttc ggattgactg ccaactctga
     5701 cacccacctg cttcaggggc agagcctgac cctgaccttg gagagccccc ctggtagtag
     5761 cccctcagtg caatgtagga gtccaagggg taaaaacata caggggggga agaccctctc
     5821 cgtgtctcag ctggagctcc aggatagtgg cacctggaca tgcactgtct tgcagaacca
     5881 gaagaaggtg gagttcaaaa tagacatcgt ggtgctagct ttccagaagg cctccagcat
     5941 agtctataag aaagaggggg aacaggtgga gttctccttc ccactcgcct ttacagttga
     6001 aaagctgacg ggcagtggcg agctgtggtg gcaggcggag agggcttcct cctccaagtc
     6061 ttggatcacc tttgacctga agaacaagga agtgtctgta aaacgggtta cccaggaccc
     6121 taagctccag atgggcaaga agctcccgct ccacctcacc ctgccccagg ccttgcctca
     6181 gtatgctggc tctggaaacc tcaccctggc ccttgaagcg aaaacaggaa agttgcatca
     6241 ggaagtgaac ctggtggtga tgagagccac tcagctccag aaaaatttga cctgtgaggt
     6301 gtggggaccc acctccccta agctgatgct gagcttgaaa ctggagaaca aggaggcaaa
     6361 ggtctcgaag cgggagaagg cggtgtgggt gctgaaccct gaggcgggga tgtggcagtg
     6421 tctgctgagt gactcgggac aggtcctgct ggaatccaac atcaaggttc tgcccacatg
     6481 gtcgaccccg gtgcagccaa tggccctgat tgtgctgggg ggcgtcgccg gcctcctgct
     6541 tttcattggg ctaggcatct tcttctgtgt caggtgccgg cactgaaggc gacctgcgga
     6601 tcccacgtca ctattgtata ctctatatta tactctatgt tatactctgt aatcctactc
     6661 aataaacgtg tcacgcctgt gaaaccgtac taagtctccc gtgtcttctt atcaccatca
     6721 ggtgacatcc tcgcccaggc tgtcaatcat gccggtatcg attccagtag caccggcccc
     6781 acgctgacaa cccactcttg cagcgttagc agcgcccctc ttaacaagcc gacccccacc
     6841 agcgtcgcgg ttactaacac tcctctcccc gacctgcagc ccaagcttgg cgtaatcatg
     6901 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6961 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7021 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7141 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7201 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7261 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7321 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7381 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7441 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7501 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7561 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7621 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7681 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7741 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7801 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7861 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7921 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7981 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8041 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8101 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8161 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8221 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8281 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8341 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8401 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8461 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8521 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8581 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8641 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8701 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8761 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8821 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8881 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8941 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9001 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     9061 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9121 tc
//
