LOCUS       sequence-357937-       13849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian co-expression of human SEPT7 fused to GFP10 and of human 
            SEPT7 fused to GFP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13849)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 13849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pTRIP TRE BI_GFP11-14-SEPT7_GFP10-14-SEPT7
FEATURES             Location/Qualifiers
     source          1..13849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    22..103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1661..2048
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     protein_bind    1742..1760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1778..1796
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1813..1831
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1849..1867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1885..1903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1920..1938
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1956..1974
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3674..3691)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3901..3920
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4082..4783
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             5066..5246
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6774..6793)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7007..7026)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7126..7148
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7186..7204)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7272..7376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7377..7445,7446..8237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7595..7614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8408..8996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8897..8916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9150..9167
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9237..9257)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9242..9571
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9422..9557
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10705..10770
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10900..10920
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11345..11479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     11436..11455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11647..12280
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12327..12452
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12945..13178
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13363..13407
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13700..13817
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttggctgata cgcgtacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
       61 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      181 ctacaaatgt ggtatggctg attatgatcc tctagattaa aagatcttcc ctttcttctt
      241 gttcttttcc aaggttcttg aagagttctg ttgttctaaa atacgttgtt gagcttccca
      301 gtttgctttc tcatcctcga actgacgacg tttttcctcc aattctttgt gctgtgcttc
      361 caaattcttt ttcatttgct catggcgccg ctggagctca gcttcagagt ccttcagttt
      421 ttgaactttt tctttgacct tcatctcaaa cacctgctcc atctccatct ccatcttctt
      481 cattttagct acatgctccc ttctttcttc ttccatttgt gccagagggc tcttagtcag
      541 ctgcccttta ttcttgttgt tatcaactcc attataagtc acagctgcaa gttttctgct
      601 tctgtagttc tcatagtgga cattattagt aacatctttc aagtcctgca tgtgtgttct
      661 tatcaacata tttcttagga ttgtaaaatc acaatgttca ccattttcaa cttcagcaac
      721 accccaagga tactgccttc ctctgaccct tttgccatta acttcaatga tagtattact
      781 acctaccaca gcaagaggta aacggtcctt tatcttttta acaagtttat tttcttcttc
      841 atcatctgtt tctggaaatt cgtatatttt aattttatgt tcttggattt ctttcattat
      901 ctgtttttta aactgttggc attcctctgg tgtgagtgtg tctgctttgg caataagtgg
      961 gatgatattc actttttcat gcaaacgctt cataaactca atatccaatg gtttaagtcc
     1021 atgtcctgaa ggagcaatga agtataaaca acactgcacc ctgttatcag gcatctgacg
     1081 tctgttcact cgtgattctg catttaggta gtcctcaaat ttactatcaa tgtagtcgat
     1141 aacaggctgc cagcaattac tattatccac tgcatctcca aatcctgggg tatcaactat
     1201 tgtgagcagc aactgaacac caccttcttt gattaaaact ttggattgtt ccacctgtac
     1261 agtcttttta attctatgag aaggacctgg atactctgga gaatacaaat ctgtgaggaa
     1321 taatgagttg attaatgtcg actttcccaa tccagattca cccactacca taagcgtgaa
     1381 ttcaaaacct ctcttcaccg attttctgta tacttgattt gggagattgg caaatcccac
     1441 atagccttca aggttcttct gttgagctac catggtgctg ctgttgacgc tcctctcctc
     1501 agcagcagcg gatctcgcac tgaccgacat tccggaggac ccaccacctc cagagccacc
     1561 gccaccatcg atggaggcgt cggtgatgcc ggcggcggtc acgtactcca gcagcaccat
     1621 gtggtcgcgc ttctccatca tatgaccggt gaattctcca ggcgatctga cggttcacta
     1681 aacgagctct gcttatatag gcctcccacc gtacacgcca cctcgacata ctcgagttta
     1741 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga
     1801 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     1861 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
     1921 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     1981 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2041 agatcgcctg gagatttcga gctcggtacc gcggatgggc gacctgcccg acgaccacta
     2101 cctgtccacc cagaccatcc tgagcaagga cctgaacatc gatggtggcg gtggctctgg
     2161 aggtggtggg tcctccggaa tgtcggtcag tgcgagatcc gctgctgctg aggagaggag
     2221 cgtcaacagc agcaccatgg tagctcaaca gaagaacctt gaaggctatg tgggatttgc
     2281 caatctccca aatcaagtat acagaaaatc ggtgaagaga ggttttgaat tcacgcttat
     2341 ggtagtgggt gaatctggat tgggaaagtc gacattaatc aactcattat tcctcacaga
     2401 tttgtattct ccagagtatc caggtccttc tcatagaatt aaaaagactg tacaggtgga
     2461 acaatccaaa gttttaatca aagaaggtgg tgttcagttg ctgctcacaa tagttgatac
     2521 cccaggattt ggagatgcag tggataatag taattgctgg cagcctgtta tcgactacat
     2581 tgatagtaaa tttgaggact acctaaatgc agaatcacga gtgaacagac gtcagatgcc
     2641 tgataacagg gtgcagtgtt gtttatactt cattgctcct tcaggacatg gacttaaacc
     2701 attggatatt gagtttatga agcgtttgca tgaaaaagtg aatatcatcc cacttattgc
     2761 caaagcagac acactcacac cagaggaatg ccaacagttt aaaaaacaga taatgaaaga
     2821 aatccaagaa cataaaatta aaatatacga atttccagaa acagatgatg aagaagaaaa
     2881 taaacttgtt aaaaagataa aggaccgttt acctcttgct gtggtaggta gtaatactat
     2941 cattgaagtt aatggcaaaa gggtcagagg aaggcagtat ccttggggtg ttgctgaagt
     3001 tgaaaatggt gaacattgtg attttacaat cctaagaaat atgttgataa gaacacacat
     3061 gcaggacttg aaagatgtta ctaataatgt ccactatgag aactacagaa gcagaaaact
     3121 tgcagctgtg acttataatg gagttgataa caacaagaat aaagggcagc tgactaagag
     3181 ccctctggca caaatggaag aagaaagaag ggagcatgta gctaaaatga agaagatgga
     3241 gatggagatg gagcaggtgt ttgagatgaa ggtcaaagaa aaagttcaaa aactgaagga
     3301 ctctgaagct gagctccagc ggcgccatga gcaaatgaaa aagaatttgg aagcacagca
     3361 caaagaattg gaggaaaaac gtcgtcagtt cgaggatgag aaagcaaact gggaagctca
     3421 acaacgtatt ttagaacaac agaactcttc aagaaccttg gaaaagaaca agaagaaagg
     3481 gaagatcttt taagctagct aatagttccg cccctctccc tcccccctaa cgttactggc
     3541 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     3601 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     3661 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3721 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3781 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3841 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     3901 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     3961 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     4021 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgcaag
     4081 catgagcgag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga
     4141 caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     4201 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     4261 tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt
     4321 caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     4381 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     4441 gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     4501 ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat
     4561 ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc
     4621 caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga
     4681 aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag
     4741 atactgcgac ctccctagca aactggggca caagcttaat taaggatcca gcggccgctc
     4801 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     4861 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     4921 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4981 ggggactgga agggctaatt cactcccaac gaagacaaaa tcgtcgagag atgctgcata
     5041 taagcagctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5101 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5161 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5221 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5281 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattata atagatttag
     5341 caggaattga actaggagtg gagcacacag gcaaagctgc agaagtactt ggaagaagcc
     5401 accagagata ctcacgattc tgcacatacc tggctaatcc cagatcctaa ggattacatt
     5461 aagtttacta acatttatat aatgatttat agtttaaagt ataaacttat ctaatttact
     5521 attctgacag atattaatta atcctcaaat atcataagag atgattacta ttatccccat
     5581 ttaacacaag aggaaactga gagggaaaga tgttgaagta attttcccac aattacagca
     5641 tccgttagtt acgactctat gatcttctga cacaaattcc atttactcct caccctatga
     5701 ctcagtcgaa tatatcaaag ttatggacat tatgctaagt aacaaattac ccttttatat
     5761 agtaaatact gagtagattg agagaagaaa ttgtttgcaa acctgaatag cttcaagaag
     5821 aagagaagtg aggataagaa taacagttgt catttaacaa gttttaacaa gtaacttggt
     5881 tagaaaggga ttcaaatgca taaagcaagg gataaatttt tctggcaaca agactataca
     5941 atataacctt aaatatgact tcaaataatt gttggaactt gataaaacta attaaatatt
     6001 attgaagatt atcaatatta taaatgtaat ttacttttaa aaagggaaca tagaaatgtg
     6061 tatcattaga gtagaaaaca atccttatta tcacaatttg tcaaaacaag tttgttatta
     6121 acacaagtag aatactgcat tcaattaagt tgactgcaga ttttgtgttt tgttaaaatt
     6181 agaaagagat aacaacaatt tgaattattg aaagtaacat gtaaatagtt ctacatacgt
     6241 tcttttgaca tcttgttcaa tcattgatcg aagttcttta tcttggaaga atttgttcca
     6301 aagactctga aataaggaaa acaatctatt atatagtctc acacctttgt tttactttta
     6361 gtgatttcaa tttaataatg taaatggtta aaatttattc ttctctgaga tcatttcaca
     6421 ttgcagatag aaaacctgag actggggtaa tttttattaa aatctaattt aatctcagaa
     6481 acacatcttt attctaacat caatttttcc agtttgatat tatcatataa agtcagcctt
     6541 cctcatctgc aggttccaca acaaaaatcc aaccaactgt ggatcaaaaa tattgggaaa
     6601 aaattaaaaa tagcaataca acaataaaaa aatacaaatc agaaaaacag cacagtataa
     6661 caactttatt tagcatttac aatctattag gtattataag taatctagcc agatcctcta
     6721 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     6781 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     6841 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     6901 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     6961 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg
     7021 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     7081 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     7141 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     7201 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     7261 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7921 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8281 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8461 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8521 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8581 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8641 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8701 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8761 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8821 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8881 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8941 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9001 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9061 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9181 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
     9241 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9301 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9361 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9421 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9481 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9541 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
     9601 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     9661 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     9721 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     9781 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     9841 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
     9901 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     9961 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
    10021 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
    10081 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
    10141 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
    10201 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
    10261 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
    10321 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
    10381 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
    10441 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
    10501 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
    10561 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
    10621 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
    10681 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
    10741 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
    10801 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
    10861 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
    10921 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
    10981 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
    11041 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
    11101 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
    11161 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
    11221 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
    11281 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    11341 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    11401 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    11461 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    11521 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    11581 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    11641 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
    11701 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
    11761 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
    11821 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
    11881 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
    11941 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
    12001 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
    12061 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
    12121 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
    12181 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
    12241 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
    12301 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
    12361 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
    12421 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
    12481 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
    12541 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
    12601 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
    12661 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
    12721 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
    12781 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
    12841 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    12901 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    12961 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    13021 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    13081 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    13141 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    13201 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    13261 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    13321 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    13381 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    13441 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    13501 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    13561 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    13621 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa
    13681 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    13741 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    13801 caaaaattca aaattttcgg gtttattaca gggacagcag agatccact
//
