LOCUS       sequence-357939-       13849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of 
            human SEPT7 Gmut2 fused to GFP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13849)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 13849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEP...
FEATURES             Location/Qualifiers
     source          1..13849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1492..1879
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     protein_bind    1573..1591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1609..1627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1644..1662
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1680..1698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1716..1734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1751..1769
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1787..1805
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3505..3522)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3732..3751
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3913..4614
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             4897..5077
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6605..6624)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6838..6857)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6957..6979
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7017..7035)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7103..7207
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7208..7276,7277..8068)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7426..7445)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8239..8827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8728..8747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8981..8998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9068..9088)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9073..9402
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9253..9388
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10536..10601
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10731..10751
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11176..11310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     11267..11286
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11478..12111
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12158..12283
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12776..13009
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13194..13238
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13531..13648
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    13702..13783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatta aaagatcttc
       61 cctttcttct tgttcttttc caaggttctt gaagagttct gttgttctaa aatacgttgt
      121 tgagcttccc agtttgcttt ctcatcctcg aactgacgac gtttttcctc caattctttg
      181 tgctgtgctt ccaaattctt tttcatttgc tcatggcgcc gctggagctc agcttcagag
      241 tccttcagtt tttgaacttt ttctttgacc ttcatctcaa acacctgctc catctccatc
      301 tccatcttct tcattttagc tacatgctcc cttctttctt cttccatttg tgccagaggg
      361 ctcttagtca gctgcccttt attcttgttg ttatcaactc cattataagt cacagctgca
      421 agttttctgc ttctgtagtt ctcatagtgg acattattag taacatcttt caagtcctgc
      481 atgtgtgttc ttatcaacat atttcttagg attgtaaaat cacaatcttc accattttca
      541 acttcagcaa caccccaagg atactgcctt cctctgaccc ttttgccatt aacttcaatg
      601 atagtattac tacctaccac agcaagaggt aaacggtcct ttatcttttt aacaagttta
      661 ttttcttctt catcatctgt ttctggaaat tcgtatattt taattttatg ttcttggatt
      721 tctttcatta tctgtttttt aaactgttgg cattcctctg gtgtgagtgt gtctgctttg
      781 gcaataagtg ggatgatatt cactttttca tgcaaacgct tcataaactc aatatccaat
      841 ggtttaagtc catgtcctga aggagcaatg aagtataaac aacactgcac cctgttatca
      901 ggcatctgac gtctgttcac tcgtgattct gcatttaggt agtcctcaaa tttactatca
      961 atgtagtcga taacaggctg ccagcaatta ctattatcca ctgcatctcc aaatcctggg
     1021 gtatcaacta ttgtgagcag caactgaaca ccaccttctt tgattaaaac tttggattgt
     1081 tccacctgta cagtcttttt aattctatga gaaggacctg gatactctgg agaatacaaa
     1141 tctgtgagga ataatgagtt gattaatgtc gactttccca atccagattc acccactacc
     1201 ataagcgtga attcaaaacc tctcttcacc gattttctgt atacttgatt tgggagattg
     1261 gcaaatccca catagccttc aaggttcttc tgttgagcta ccatggtgct gctgttgacg
     1321 ctcctctcct cagcagcagc ggatctcgca ctgaccgaca ttccggagga cccaccacct
     1381 ccagagccac cgccaccatc gatggaggcg tcggtgatgc cggcggcggt cacgtactcc
     1441 agcagcacca tgtggtcgcg cttctccatc atatgaccgg tgaattctcc aggcgatctg
     1501 acggttcact aaacgagctc tgcttatata ggcctcccac cgtacacgcc acctcgacat
     1561 actcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     1621 atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
     1681 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     1741 gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     1801 agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
     1861 agtgaaccgt cagatcgcct ggagatttcg agctcggtac cgcggatggg cgacctgccc
     1921 gacgaccact acctgtccac ccagaccatc ctgagcaagg acctgaacat cgatggtggc
     1981 ggtggctctg gaggtggtgg gtcctccgga atgtcggtca gtgcgagatc cgctgctgct
     2041 gaggagagga gcgtcaacag cagcaccatg gtagctcaac agaagaacct tgaaggctat
     2101 gtgggatttg ccaatctccc aaatcaagta tacagaaaat cggtgaagag aggttttgaa
     2161 ttcacgctta tggtagtggg tgaatctgga ttgggaaagt cgacattaat caactcatta
     2221 ttcctcacag atttgtattc tccagagtat ccaggtcctt ctcatagaat taaaaagact
     2281 gtacaggtgg aacaatccaa agttttaatc aaagaaggtg gtgttcagtt gctgctcaca
     2341 atagttgata ccccaggatt tggagatgca gtggataata gtaattgctg gcagcctgtt
     2401 atcgactaca ttgatagtaa atttgaggac tacctaaatg cagaatcacg agtgaacaga
     2461 cgtcagatgc ctgataacag ggtgcagtgt tgtttatact tcattgctcc ttcaggacat
     2521 ggacttaaac cattggatat tgagtttatg aagcgtttgc atgaaaaagt gaatatcatc
     2581 ccacttattg ccaaagcaga cacactcaca ccagaggaat gccaacagtt taaaaaacag
     2641 ataatgaaag aaatccaaga acataaaatt aaaatatacg aatttccaga aacagatgat
     2701 gaagaagaaa ataaacttgt taaaaagata aaggaccgtt tacctcttgc tgtggtaggt
     2761 agtaatacta tcattgaagt taatggcaaa agggtcagag gaaggcagta tccttggggt
     2821 gttgctgaag ttgaaaatgg tgaagattgt gattttacaa tcctaagaaa tatgttgata
     2881 agaacacaca tgcaggactt gaaagatgtt actaataatg tccactatga gaactacaga
     2941 agcagaaaac ttgcagctgt gacttataat ggagttgata acaacaagaa taaagggcag
     3001 ctgactaaga gccctctggc acaaatggaa gaagaaagaa gggagcatgt agctaaaatg
     3061 aagaagatgg agatggagat ggagcaggtg tttgagatga aggtcaaaga aaaagttcaa
     3121 aaactgaagg actctgaagc tgagctccag cggcgccatg agcaaatgaa aaagaatttg
     3181 gaagcacagc acaaagaatt ggaggaaaaa cgtcgtcagt tcgaggatga gaaagcaaac
     3241 tgggaagctc aacaacgtat tttagaacaa cagaactctt caagaacctt ggaaaagaac
     3301 aagaagaaag ggaagatctt ttaagctagc taatagttcc gcccctctcc ctccccccta
     3361 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     3421 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     3481 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     3541 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     3601 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     3661 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     3721 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     3781 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     3841 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     3901 cacgatgcaa gcatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag
     3961 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     4021 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     4081 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
     4141 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     4201 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     4261 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     4321 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
     4381 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc
     4441 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     4501 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
     4561 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa ttaaggatcc
     4621 agcggccgct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     4681 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     4741 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4801 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaaa atcgtcgaga
     4861 gatgctgcat ataagcagct gctttttgct tgtactgggt ctctctggtt agaccagatc
     4921 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     4981 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5041 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     5101 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattat
     5161 aatagattta gcaggaattg aactaggagt ggagcacaca ggcaaagctg cagaagtact
     5221 tggaagaagc caccagagat actcacgatt ctgcacatac ctggctaatc ccagatccta
     5281 aggattacat taagtttact aacatttata taatgattta tagtttaaag tataaactta
     5341 tctaatttac tattctgaca gatattaatt aatcctcaaa tatcataaga gatgattact
     5401 attatcccca tttaacacaa gaggaaactg agagggaaag atgttgaagt aattttccca
     5461 caattacagc atccgttagt tacgactcta tgatcttctg acacaaattc catttactcc
     5521 tcaccctatg actcagtcga atatatcaaa gttatggaca ttatgctaag taacaaatta
     5581 cccttttata tagtaaatac tgagtagatt gagagaagaa attgtttgca aacctgaata
     5641 gcttcaagaa gaagagaagt gaggataaga ataacagttg tcatttaaca agttttaaca
     5701 agtaacttgg ttagaaaggg attcaaatgc ataaagcaag ggataaattt ttctggcaac
     5761 aagactatac aatataacct taaatatgac ttcaaataat tgttggaact tgataaaact
     5821 aattaaatat tattgaagat tatcaatatt ataaatgtaa tttactttta aaaagggaac
     5881 atagaaatgt gtatcattag agtagaaaac aatccttatt atcacaattt gtcaaaacaa
     5941 gtttgttatt aacacaagta gaatactgca ttcaattaag ttgactgcag attttgtgtt
     6001 ttgttaaaat tagaaagaga taacaacaat ttgaattatt gaaagtaaca tgtaaatagt
     6061 tctacatacg ttcttttgac atcttgttca atcattgatc gaagttcttt atcttggaag
     6121 aatttgttcc aaagactctg aaataaggaa aacaatctat tatatagtct cacacctttg
     6181 ttttactttt agtgatttca atttaataat gtaaatggtt aaaatttatt cttctctgag
     6241 atcatttcac attgcagata gaaaacctga gactggggta atttttatta aaatctaatt
     6301 taatctcaga aacacatctt tattctaaca tcaatttttc cagtttgata ttatcatata
     6361 aagtcagcct tcctcatctg caggttccac aacaaaaatc caaccaactg tggatcaaaa
     6421 atattgggaa aaaattaaaa atagcaatac aacaataaaa aaatacaaat cagaaaaaca
     6481 gcacagtata acaactttat ttagcattta caatctatta ggtattataa gtaatctagc
     6541 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     6601 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     6661 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     6721 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     6781 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac
     6841 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     6901 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     6961 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     7021 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7081 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7141 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7201 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7261 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7321 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7381 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7441 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7501 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7561 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7621 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7681 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7741 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7801 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7861 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7921 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7981 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8041 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8101 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8161 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8221 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8281 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8341 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8401 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8461 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8521 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8581 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8641 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8701 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8761 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8821 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8881 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8941 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9001 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9061 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     9121 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     9181 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     9241 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9301 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9361 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca
     9421 ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta
     9481 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc
     9541 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
     9601 catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc
     9661 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
     9721 gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
     9781 tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat
     9841 ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt
     9901 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
     9961 ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat
    10021 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg
    10081 ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga
    10141 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
    10201 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
    10261 ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
    10321 ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga
    10381 tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg
    10441 gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat
    10501 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
    10561 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
    10621 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
    10681 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
    10741 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
    10801 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
    10861 cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca
    10921 gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta
    10981 ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg
    11041 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
    11101 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    11161 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    11221 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    11281 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    11341 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    11401 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    11461 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
    11521 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
    11581 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
    11641 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
    11701 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
    11761 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
    11821 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
    11881 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
    11941 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    12001 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    12061 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
    12121 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
    12181 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    12241 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    12301 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
    12361 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    12421 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    12481 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    12541 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    12601 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
    12661 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
    12721 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
    12781 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
    12841 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
    12901 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
    12961 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
    13021 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
    13081 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
    13141 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
    13201 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
    13261 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
    13321 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
    13381 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
    13441 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
    13501 gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
    13561 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
    13621 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
    13681 ttggctgata cgcgtacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
    13741 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
    13801 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagc
//
