LOCUS       sequence-357952-        1833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For production of AAV for a fluorescent sensor for oxytocin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ino D, Tanaka Y, Hibino H, Nishiyama M
  TITLE     A fluorescent sensor for real-time measurement of extracellular 
            oxytocin dynamics in the brain.
  JOURNAL   Nat Methods. 2022 Sep 22. pii: 10.1038/s41592-022-01597-x. doi: 
            10.1038/s41592-022-01597-x.
  PUBMED    36138174
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 Sep 22. pii: 10.1038/s41592-022-01597-x. doi: 
            10.1038/s41592-022-01597-x."
COMMENT     Sequence Label: pAAV.EF1a.FLEX.MTRIA-OT
FEATURES             Location/Qualifiers
     source          1..1833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     930..951
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1057..1078)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atggagatca tttccaatga aagtgagatc tggcagttca acggatcgtg gaggaactca
       61 agcctcggta acggcaccgg cgctctgaat cagacgaacc ccctgaagcg gaacgaggag
      121 gtggcgaagg tggaggtgac ggtgctggcg ctggtgctgt ttctggcgct ggcggggaac
      181 ctgtgcgtcc tgctggccat ccacaccacc aaacacagcc agtcccgcat gtattacttc
      241 atgaagcacc tgagcatcgc cgatctagtt gtggcaatat ttcaagttct cccccaactt
      301 atctgggaca tcacattccg gttttatgga cctgacattt tgtgcaggtt ggtgaaatac
      361 ctgcaggtgg ttggaatgtt cgcctccacg tacatgttag ttttaatgtc catcgacaga
      421 tgtttggcca tctgtcagcc tctgcactcc ttgcatcgga gaaaagatcg aatttatgtc
      481 atattgtcat ggttgctgag tttgatcttc agcatcccgc agatgttcat cttctccctc
      541 agagaggttg gctcggccgg gtcaggagtg tacgactgct ggggcgactt tgtgaagccc
      601 tggggggcca aagcttacat cacatggatc agtctcacca tctacatcat cccagtggcc
      661 atcctgagca tttgttacgg gctgatctgc tacaaaatat ggcgaaattt caaagggaaa
      721 ttaaacgtct atatcaaggc cgacaagcag aagaacggca tcaaggcgaa cttccacatc
      781 cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc actaccagca gaacaccccc
      841 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcgtgca gaccaaactt
      901 tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      961 gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggat gatggtgaga
     1021 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1081 aacggccaca agttcagcgt gtccggcgag ggtgagggcg atgccaccta cggcaagctg
     1141 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1201 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     1261 ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt cttcaaggac
     1321 gacggccact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1381 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1441 tacaactcag taatctccaa ggcaaagata cgcacagtga aaatgacttt tgtgatagtc
     1501 gtggcataca tcgtctgctg gaccccgttt ttctctgtcc agatgtggtc tgcctgggat
     1561 caagcggctc cccgtgaggc catgcccttc atcatctcca tgctgctggc gagcctgaac
     1621 agctgctgca acccctggat ctacatgtgc tttgccggcc acctcttcca tgaccttcgg
     1681 cagaacctcc tgtgctgctc cactcgctac ctcaagtcac ccgagtgccg ctgcgagcga
     1741 gatttcaatt ctagtcacaa aagcaactcc tcaaacttcg ccattaagag cacaagcagc
     1801 agcagaagca ttactcagac ttccaccaca tga
//
