LOCUS       sequence-357978-        1899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of a fluorescent sensor for norepinephrine in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ino D, Tanaka Y, Hibino H, Nishiyama M
  TITLE     A fluorescent sensor for real-time measurement of extracellular 
            oxytocin dynamics in the brain.
  JOURNAL   Nat Methods. 2022 Sep 22. pii: 10.1038/s41592-022-01597-x. doi: 
            10.1038/s41592-022-01597-x.
  PUBMED    36138174
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 Sep 22. pii: 10.1038/s41592-022-01597-x. doi: 
            10.1038/s41592-022-01597-x."
COMMENT     Sequence Label: pCIS.MTRIA-NE
FEATURES             Location/Qualifiers
     source          1..1899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     909..930
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1036..1057)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(1597..1608)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atggggccac acgggaacga cagcgacttc ttgctggcac ccaacggaag ccgagcgcca
       61 gaccacgacg tcactcagga acgggacgaa gcgtgggttg tgggcatggc catcctcatg
      121 tcggttatcg tcctggccat cgtgtttggc aacgtgctgg tcatcacagc cattgccaag
      181 ttcgagcgac tacaaaccgt caccaactac ttcataatct ccttggcgtg tgctgatcta
      241 gtcatgggcc tagcggtggt gccgtttggg gccagtcaca tccttatgaa aatgtggaat
      301 tttggcaact tctggtgcga gttctggact tccattgatg tgttgtgcgt cacagccagc
      361 atcgagaccc tgtgcgtgat tgcagtggat cgctatgttg ctatcacatc gcccttcaag
      421 taccagagcc tgctgaccaa gaataaggcc cgagtggtca tcctgatggt atggattgta
      481 tctggcctta cctccttttt gcctatccag atgcactggt accgtgccac ccacaagaaa
      541 gctatcgatt gttacaccga ggagacttgc tgtgacttct tcacgaacca ggcctacgcc
      601 atcgcgtcct cgattgtgtc tttctacgtg cccctggtgg tgatggtctt tgtctattcc
      661 cgggtctgct acaaaatatg gcgaaatttc aaagggaaat taaacgtcta tatcaaggcc
      721 gacaagcaga agaacggcat caaggcgaac ttccacatcc gccacaacat cgaggacggc
      781 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      841 ctgcccgaca accactacct gagcgtgcag accaaacttt cgaaagaccc caacgagaag
      901 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      961 gagctgtaca agggcggtac cggagggatg atggtgagaa agggcgagga gctgttcacc
     1021 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1081 tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1141 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1201 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1261 gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggccacta caagacccgc
     1321 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1381 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactcagt aatctccaag
     1441 gcaaagatac gcacagtgaa aatgttaggc atcatcatgg gcacattcac cctctgctgg
     1501 ctgcccttct tcattgtcaa tatcgtgcac gttatcaggg acaacctcat ccctaaggaa
     1561 gtttacatcc tccttaactg gttgggctac gtcaactctg ccttcaatcc tcttatctac
     1621 tgtcggagtc cagatttcag gattgccttt caagagcttc tgtgccttcg caggtcttct
     1681 tcgaaaacct atgggaacgg ctactctagc aatagcaacg gcagaacgga ctacacaggg
     1741 gagccaaaca cttgtcagct ggggcaggag agagaacagg aactgctgtg tgaggatccc
     1801 ccaggcatgg aaggctttgt gaactgtcaa ggtactgtgc ctagccttag cgttgactcc
     1861 caaggaagga actgtagtac aaatgactcg ccactgtaa
//
