LOCUS       sequence-358006-       14242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian co-expression of human SEPT9_i3 fused to GFP10 and of 
            human SEPT7 Gmut2 fused to GFP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14242)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 14242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 ...
FEATURES             Location/Qualifiers
     source          1..14242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1492..1879
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     protein_bind    1573..1591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1609..1627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1644..1662
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1680..1698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1716..1734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1751..1769
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1787..1805
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3898..3915)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4125..4144
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4306..5007
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             5290..5470
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6998..7017)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7231..7250)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7350..7372
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7410..7428)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7496..7600
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7601..7669,7670..8461)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7819..7838)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8632..9220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9121..9140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9374..9391
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9461..9481)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9466..9795
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9646..9781
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10929..10994
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11124..11144
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11569..11703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     11660..11679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11871..12504
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12551..12676
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13169..13402
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13587..13631
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13924..14041
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    14095..14176
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatta aaagatcttc
       61 cctttcttct tgttcttttc caaggttctt gaagagttct gttgttctaa aatacgttgt
      121 tgagcttccc agtttgcttt ctcatcctcg aactgacgac gtttttcctc caattctttg
      181 tgctgtgctt ccaaattctt tttcatttgc tcatggcgcc gctggagctc agcttcagag
      241 tccttcagtt tttgaacttt ttctttgacc ttcatctcaa acacctgctc catctccatc
      301 tccatcttct tcattttagc tacatgctcc cttctttctt cttccatttg tgccagaggg
      361 ctcttagtca gctgcccttt attcttgttg ttatcaactc cattataagt cacagctgca
      421 agttttctgc ttctgtagtt ctcatagtgg acattattag taacatcttt caagtcctgc
      481 atgtgtgttc ttatcaacat atttcttagg attgtaaaat cacaatcttc accattttca
      541 acttcagcaa caccccaagg atactgcctt cctctgaccc ttttgccatt aacttcaatg
      601 atagtattac tacctaccac agcaagaggt aaacggtcct ttatcttttt aacaagttta
      661 ttttcttctt catcatctgt ttctggaaat tcgtatattt taattttatg ttcttggatt
      721 tctttcatta tctgtttttt aaactgttgg cattcctctg gtgtgagtgt gtctgctttg
      781 gcaataagtg ggatgatatt cactttttca tgcaaacgct tcataaactc aatatccaat
      841 ggtttaagtc catgtcctga aggagcaatg aagtataaac aacactgcac cctgttatca
      901 ggcatctgac gtctgttcac tcgtgattct gcatttaggt agtcctcaaa tttactatca
      961 atgtagtcga taacaggctg ccagcaatta ctattatcca ctgcatctcc aaatcctggg
     1021 gtatcaacta ttgtgagcag caactgaaca ccaccttctt tgattaaaac tttggattgt
     1081 tccacctgta cagtcttttt aattctatga gaaggacctg gatactctgg agaatacaaa
     1141 tctgtgagga ataatgagtt gattaatgtc gactttccca atccagattc acccactacc
     1201 ataagcgtga attcaaaacc tctcttcacc gattttctgt atacttgatt tgggagattg
     1261 gcaaatccca catagccttc aaggttcttc tgttgagcta ccatggtgct gctgttgacg
     1321 ctcctctcct cagcagcagc ggatctcgca ctgaccgaca ttccggagga cccaccacct
     1381 ccagagccac cgccaccatc gatggaggcg tcggtgatgc cggcggcggt cacgtactcc
     1441 agcagcacca tgtggtcgcg cttctccatc atatgaccgg tgaattctcc aggcgatctg
     1501 acggttcact aaacgagctc tgcttatata ggcctcccac cgtacacgcc acctcgacat
     1561 actcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     1621 atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
     1681 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     1741 gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     1801 agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
     1861 agtgaaccgt cagatcgcct ggagatttcg agctcggtac cgcggatgga gagggaccgg
     1921 atctcagcct tgaaaagatc ttttgaggtc gaggaggtcg agacacccaa ctccacccca
     1981 ccccggaggg tccagactcc cctactccga gccactgtgg ccagctccac ccagaaattc
     2041 caggacctgg gcgtgaagaa ctcagaaccc tcggcccgcc atgtggactc cctaagccaa
     2101 cgctccccca aggcgtccct gcggagggtg gagctctcgg gccccaaggc ggccgagccg
     2161 gtgtcccggc gcactgagct gtccattgac atctcgtcca agcaggtgga gaacgccggg
     2221 gccatcggcc cgtcccggtt cgggctcaag agggccgagg tgttgggcca caagacgcca
     2281 gaaccggccc ctcggaggac ggagatcacc atcgtcaaac cccaggagtc agcccaccgg
     2341 aggatggagc cccctgcctc caaggtcccc gaggtgccca ctgcccctgc caccgacgca
     2401 gcccccaaga gggtggagat ccagatgccc aagcctgctg aggcgcccac cgcccccagc
     2461 ccagcccaga ccttggaaaa ttcagagcct gcccctgtgt ctcagctgca gagcaggctg
     2521 gagcccaagc cccagccccc tgtggctgag gctacacccc ggagccagga ggccactgag
     2581 gcggctccca gctgcgttgg cgacatggcc gacaccccca gagatgccgg gctcaagcag
     2641 gcgcctgcat cacggaacga gaaggccccg gtggacttcg gctacgtggg gattgactcc
     2701 atcctggagc agatgcgccg gaaggccatg aagcagggct tcgagttcaa catcatggtg
     2761 gtcgggcaga gcggcttggg taaatccacc ttaatcaaca ccctcttcaa atccaaaatc
     2821 agccggaagt cggtgcagcc cacctcagag gagcgcatcc ccaagaccat cgagatcaag
     2881 tccatcacgc acgatattga ggagaaaggc gtccggatga agctgacagt gattgacaca
     2941 ccagggttcg gggaccacat caacaacgag aactgctggc agcccatcat gaagttcatc
     3001 aatgaccagt acgagaaata cctgcaggag gaggtcaaca tcaaccgcaa gaagcgcatc
     3061 ccggacaccc gcgtccactg ctgcctctac ttcatccccg ccaccggcca ctccctcagg
     3121 cccctggaca tcgagtttat gaaacgcctg agcaaggtgg tcaacatcgt ccctgtcatc
     3181 gccaaggcgg acacactcac cctggaggag agggtccact tcaaacagcg gatcaccgca
     3241 gacctgctgt ccaacggcat cgacgtgtac ccccagaagg aatttgatga ggactcggag
     3301 gaccggctgg tgaacgagaa gttccgggag atgatcccat ttgctgtggt gggcagtgac
     3361 cacgagtacc aggtcaacgg caagaggatc cttgggagga agaccaagtg gggtaccatc
     3421 gaagttgaaa acaccacaca ctgtgagttt gcctacctgc gggaccttct catcaggacg
     3481 cacatgcaga acatcaagga catcaccagc agcatccact tcgaggcgta ccgtgtgaag
     3541 cgcctcaacg agggcagcag cgccatggcc aacggcgtgg aggagaagga gccagaagcc
     3601 ccagagatga tcgatggtgg cggtggctct ggaggtggtg ggtcctccgg aatgggcgac
     3661 ctgcccgacg accactacct gtccacccag accatcctga gcaaggacct gaactaagct
     3721 agctaatagt tccgcccctc tccctccccc ctaacgttac tggccgaagc cgcttggaat
     3781 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     3841 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     3901 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     3961 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     4021 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     4081 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     4141 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     4201 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     4261 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg caagcatgag cgagctgatt
     4321 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag
     4381 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
     4441 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc
     4501 agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct
     4561 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
     4621 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     4681 ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag
     4741 acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg
     4801 ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag
     4861 aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc
     4921 aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct
     4981 agcaaactgg ggcacaagct taattaagga tccagcggcc gctcgagacc tagaaaaaca
     5041 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     5101 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     5161 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5221 aattcactcc caacgaagac aaaatcgtcg agagatgctg catataagca gctgcttttt
     5281 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5341 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5401 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5461 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     5521 tgaatatcag agagtgagag gccttgacat tataatagat ttagcaggaa ttgaactagg
     5581 agtggagcac acaggcaaag ctgcagaagt acttggaaga agccaccaga gatactcacg
     5641 attctgcaca tacctggcta atcccagatc ctaaggatta cattaagttt actaacattt
     5701 atataatgat ttatagttta aagtataaac ttatctaatt tactattctg acagatatta
     5761 attaatcctc aaatatcata agagatgatt actattatcc ccatttaaca caagaggaaa
     5821 ctgagaggga aagatgttga agtaattttc ccacaattac agcatccgtt agttacgact
     5881 ctatgatctt ctgacacaaa ttccatttac tcctcaccct atgactcagt cgaatatatc
     5941 aaagttatgg acattatgct aagtaacaaa ttaccctttt atatagtaaa tactgagtag
     6001 attgagagaa gaaattgttt gcaaacctga atagcttcaa gaagaagaga agtgaggata
     6061 agaataacag ttgtcattta acaagtttta acaagtaact tggttagaaa gggattcaaa
     6121 tgcataaagc aagggataaa tttttctggc aacaagacta tacaatataa ccttaaatat
     6181 gacttcaaat aattgttgga acttgataaa actaattaaa tattattgaa gattatcaat
     6241 attataaatg taatttactt ttaaaaaggg aacatagaaa tgtgtatcat tagagtagaa
     6301 aacaatcctt attatcacaa tttgtcaaaa caagtttgtt attaacacaa gtagaatact
     6361 gcattcaatt aagttgactg cagattttgt gttttgttaa aattagaaag agataacaac
     6421 aatttgaatt attgaaagta acatgtaaat agttctacat acgttctttt gacatcttgt
     6481 tcaatcattg atcgaagttc tttatcttgg aagaatttgt tccaaagact ctgaaataag
     6541 gaaaacaatc tattatatag tctcacacct ttgttttact tttagtgatt tcaatttaat
     6601 aatgtaaatg gttaaaattt attcttctct gagatcattt cacattgcag atagaaaacc
     6661 tgagactggg gtaattttta ttaaaatcta atttaatctc agaaacacat ctttattcta
     6721 acatcaattt ttccagtttg atattatcat ataaagtcag ccttcctcat ctgcaggttc
     6781 cacaacaaaa atccaaccaa ctgtggatca aaaatattgg gaaaaaatta aaaatagcaa
     6841 tacaacaata aaaaaataca aatcagaaaa acagcacagt ataacaactt tatttagcat
     6901 ttacaatcta ttaggtatta taagtaatct agccagatcc tctacgccgg acgcatcgtg
     6961 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     7021 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     7081 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     7141 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     7201 aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7261 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7321 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7381 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7441 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7501 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7561 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7621 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7681 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7741 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7801 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7861 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7921 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7981 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8041 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8101 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8161 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8221 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8281 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8341 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8401 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8461 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8521 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8581 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8641 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8701 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8761 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8821 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8881 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8941 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9001 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9061 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9121 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9181 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9241 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9301 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9361 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9421 gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg
     9481 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9541 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9601 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9661 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9721 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9781 ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc
     9841 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
     9901 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
     9961 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
    10021 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
    10081 gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa
    10141 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
    10201 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
    10261 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt
    10321 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc
    10381 ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
    10441 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
    10501 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
    10561 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
    10621 ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca
    10681 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat
    10741 cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata
    10801 cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg
    10861 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
    10921 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
    10981 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
    11041 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
    11101 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
    11161 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
    11221 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
    11281 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
    11341 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
    11401 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
    11461 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
    11521 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    11581 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    11641 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    11701 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
    11761 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
    11821 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
    11881 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
    11941 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
    12001 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
    12061 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
    12121 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
    12181 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
    12241 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
    12301 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
    12361 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    12421 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    12481 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
    12541 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
    12601 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
    12661 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
    12721 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
    12781 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
    12841 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
    12901 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
    12961 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
    13021 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    13081 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    13141 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    13201 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    13261 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    13321 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    13381 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    13441 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    13501 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    13561 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    13621 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    13681 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    13741 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    13801 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    13861 ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag tattcatcca
    13921 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
    13981 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
    14041 tcgggtttat tacagggaca gcagagatcc actttggctg atacgcgtac gtcggcagtg
    14101 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
    14161 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
    14221 ggtgtgggag gttttttaaa gc
//
