LOCUS       sequence-358016-       14740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian co-expression of human SEPT9_i1 NCmut fused to GFP10 and 
            of human SEPT9_i1 NCmut fused to GFP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14740)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 14740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pTRIP TRE BI_SEPT9_i1 NCmut-14-GFP11_SEPT9_i1
            ...
FEATURES             Location/Qualifiers
     source          1..14740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    22..103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2105..2492
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     protein_bind    2186..2204
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2222..2240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2257..2275
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2293..2311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2329..2347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2364..2382
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2400..2418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(4565..4582)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4792..4811
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4973..5674
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             5957..6137
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7665..7684)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7898..7917)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8017..8039
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8077..8095)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8163..8267
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8268..8336,8337..9128)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8486..8505)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9299..9887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9788..9807
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10041..10058
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10128..10148)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10133..10462
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10313..10448
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11596..11661
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11791..11811
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12236..12370
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     12327..12346
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             12538..13171
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13218..13343
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13836..14069
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14254..14298
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14591..14708
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttggctgata cgcgtacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
       61 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      181 ctacaaatgt ggtatggctg attatgatcc tctagattag gaggcgtcgg tgatgccggc
      241 ggcggtcacg tactccagca gcaccatgtg gtcgcgcttc tctccggagg acccaccacc
      301 tccagagcca ccgccaccat cgatcatctc tggggcttct ggctccttct cctccacgcc
      361 gttggccatg gcgctgctgc cctcgttgag gcgcttcaca cggtacgcct cgaagtggat
      421 gctgctggtg atgtccttga tgttctgcat gtgcgtcctg atgagaaggt cccgcaggta
      481 ggcaaactca cagtgtgtgg tgttttcaac ttcgatggta ccccacttgg tcttcctccc
      541 aaggatcctc ttgccgttga cctggtactc gtggtcactg cccaccacag caaatgggat
      601 catctcccgg aacttctcgt tcaccagccg gtcctccgag tcctcatcaa attccttctg
      661 ggggtacacg tcgatgccgt tggacagcag gtctgcggtg atccgctgtt tgaagtggac
      721 cctctcctcc agggtgagtg tgtccgcctt ggcgatgaca gggacgatgt tgaccacctt
      781 gctcaggcgt ttcataaact cgatgtccag gggcctgagg gagtggccgg tggcggggat
      841 gaagtagagg cagcagtgga cgcgggtgtc cgggatgcgc ttcttgcggt tgatgttgac
      901 ctcctcctgc aggtatttct cgtactggtc attgatgaac ttcatgatgg gctgccagca
      961 gttctcgttg ttgatgtggt ccccgaaccc tggtgtgtca atcactgtca gcttcatccg
     1021 gacgcctttc tcctcaatat cgtgcgtgat ggacttgatc tcgatggtct tggggatgcg
     1081 ctcctctgag gtgggctgca ccgacttccg gctgattttg gatttgaaga gggtgttgat
     1141 taaggtggat ttacccaagc cgctctgccc gaccaccatg atgttgaact cgaagccctg
     1201 cttcatggcc ttccggcgat cctgctccag gatggagtca tcccccacgt agccgaagtc
     1261 caccggggcc ttctcgttcc gtgatgcagg cgcctgcttg agcccggcat ctctgggggt
     1321 gtcggccatg tcgccaacgc agctgggagc cgcctcagtg gcctcctggc tccggggtgt
     1381 agcctcagcc acagggggct ggggcttggg ctccagcctg ctctgcagct gagacacagg
     1441 ggcaggctct gaattctcca aggtctgggc tgggctgggg gcggtgggcg cctcagcagg
     1501 cttgggcatc tggatctcca ccctcttggg ggctgcgtcg gtggcagggg cagtgggcac
     1561 ctcggggacc ttggaggcag ggggctccat cctccggtgg gctgactcct ggggtttgac
     1621 gatggtgatc tccgtcctcc gaggggccgg ttctggcgtc ttgtggccca acacctcggc
     1681 cctcttgagc ccgaaccggg acgggccgat ggccccggcg ttctccacct gcttggacga
     1741 gatgtcaatg gacagctcag tgcgccggga caccggctcg gccgccttgg ggcccgagag
     1801 ctccaccctc cgcagggacg ccttggggga gcgttggctt agggagtcca catggcgggc
     1861 cgagggttct gagttcttca cgcccaggtc ctggaatttc tgggtggagc tggccacagt
     1921 ggctcggagt aggggagtct ggaccctccg gggtggggtg gagttgggtg tctcgacctc
     1981 ctcgacctca aaagatcttt ttaaggctgg gccactggag tcaccaagcc tccggagccg
     2041 gccactggag gtccgcgtgc ctcctgagta agacttcttc atcatatgac cggtgaattc
     2101 tccaggcgat ctgacggttc actaaacgag ctctgcttat ataggcctcc caccgtacac
     2161 gccacctcga catactcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     2221 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta
     2281 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     2341 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact
     2401 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa
     2461 gcagagctcg tttagtgaac cgtcagatcg cctggagatt tcgagctcgg taccgcggat
     2521 gaagaagtct tactcaggag gcacgcggac ctccagtggc cggctccgga ggcttggtga
     2581 ctccagtggc ccagccttaa aaagatcttt tgaggtcgag gaggtcgaga cacccaactc
     2641 caccccaccc cggagggtcc agactcccct actccgagcc actgtggcca gctccaccca
     2701 gaaattccag gacctgggcg tgaagaactc agaaccctcg gcccgccatg tggactccct
     2761 aagccaacgc tcccccaagg cgtccctgcg gagggtggag ctctcgggcc ccaaggcggc
     2821 cgagccggtg tcccggcgca ctgagctgtc cattgacatc tcgtccaagc aggtggagaa
     2881 cgccggggcc atcggcccgt cccggttcgg gctcaagagg gccgaggtgt tgggccacaa
     2941 gacgccagaa ccggcccctc ggaggacgga gatcaccatc gtcaaacccc aggagtcagc
     3001 ccaccggagg atggagcccc ctgcctccaa ggtccccgag gtgcccactg cccctgccac
     3061 cgacgcagcc cccaagaggg tggagatcca gatgcccaag cctgctgagg cgcccaccgc
     3121 ccccagccca gcccagacct tggagaattc agagcctgcc cctgtgtctc agctgcagag
     3181 caggctggag cccaagcccc agccccctgt ggctgaggct acaccccgga gccaggaggc
     3241 cactgaggcg gctcccagct gcgttggcga catggccgac acccccagag atgccgggct
     3301 caagcaggcg cctgcatcac ggaacgagaa ggccccggtg gacttcggct acgtggggga
     3361 tgactccatc ctggagcagg atcgccggaa ggccatgaag cagggcttcg agttcaacat
     3421 catggtggtc gggcagagcg gcttgggtaa atccacctta atcaacaccc tcttcaaatc
     3481 caaaatcagc cggaagtcgg tgcagcccac ctcagaggag cgcatcccca agaccatcga
     3541 gatcaagtcc atcacgcacg atattgagga gaaaggcgtc cggatgaagc tgacagtgat
     3601 tgacacacca gggttcgggg accacatcaa caacgagaac tgctggcagc ccatcatgaa
     3661 gttcatcaat gaccagtacg agaaatacct gcaggaggag gtcaacatca accgcaagaa
     3721 gcgcatcccg gacacccgcg tccactgctg cctctacttc atccccgcca ccggccactc
     3781 cctcaggccc ctggacatcg agtttatgaa acgcctgagc aaggtggtca acatcgtccc
     3841 tgtcatcgcc aaggcggaca cactcaccct ggaggagagg gtccacttca aacagcggat
     3901 caccgcagac ctgctgtcca acggcatcga cgtgtacccc cagaaggaat ttgatgagga
     3961 ctcggaggac cggctggtga acgagaagtt ccgggagatg atcccatttg ctgtggtggg
     4021 cagtgaccac gagtaccagg tcaacggcaa gaggatcctt gggaggaaga ccaagtgggg
     4081 taccatcgaa gttgaaaaca ccacacactg tgagtttgcc tacctgcggg accttctcat
     4141 caggacgcac atgcagaaca tcaaggacat caccagcagc atccacttcg aggcgtaccg
     4201 tgtgaagcgc ctcaacgagg gcagcagcgc catggccaac ggcgtggagg agaaggagcc
     4261 agaagcccca gagatgatcg atggtggcgg tggctctgga ggtggtgggt cctccggaat
     4321 gggcgacctg cccgacgacc actacctgtc cacccagacc atcctgagca aggacctgaa
     4381 ctaagctagc taatagttcc gcccctctcc ctccccccta acgttactgg ccgaagccgc
     4441 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     4501 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     4561 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     4621 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     4681 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     4741 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     4801 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     4861 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     4921 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgcaa gcatgagcga
     4981 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca
     5041 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
     5101 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct
     5161 ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc
     5221 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
     5281 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
     5341 ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt
     5401 caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa
     5461 gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc
     5521 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
     5581 ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga
     5641 cctccctagc aaactggggc acaagcttaa ttaaggatcc agcggccgct cgagacctag
     5701 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     5761 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     5821 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     5881 aagggctaat tcactcccaa cgaagacaaa atcgtcgaga gatgctgcat ataagcagct
     5941 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6001 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6061 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6121 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6181 aaagaaatga atatcagaga gtgagaggcc ttgacattat aatagattta gcaggaattg
     6241 aactaggagt ggagcacaca ggcaaagctg cagaagtact tggaagaagc caccagagat
     6301 actcacgatt ctgcacatac ctggctaatc ccagatccta aggattacat taagtttact
     6361 aacatttata taatgattta tagtttaaag tataaactta tctaatttac tattctgaca
     6421 gatattaatt aatcctcaaa tatcataaga gatgattact attatcccca tttaacacaa
     6481 gaggaaactg agagggaaag atgttgaagt aattttccca caattacagc atccgttagt
     6541 tacgactcta tgatcttctg acacaaattc catttactcc tcaccctatg actcagtcga
     6601 atatatcaaa gttatggaca ttatgctaag taacaaatta cccttttata tagtaaatac
     6661 tgagtagatt gagagaagaa attgtttgca aacctgaata gcttcaagaa gaagagaagt
     6721 gaggataaga ataacagttg tcatttaaca agttttaaca agtaacttgg ttagaaaggg
     6781 attcaaatgc ataaagcaag ggataaattt ttctggcaac aagactatac aatataacct
     6841 taaatatgac ttcaaataat tgttggaact tgataaaact aattaaatat tattgaagat
     6901 tatcaatatt ataaatgtaa tttactttta aaaagggaac atagaaatgt gtatcattag
     6961 agtagaaaac aatccttatt atcacaattt gtcaaaacaa gtttgttatt aacacaagta
     7021 gaatactgca ttcaattaag ttgactgcag attttgtgtt ttgttaaaat tagaaagaga
     7081 taacaacaat ttgaattatt gaaagtaaca tgtaaatagt tctacatacg ttcttttgac
     7141 atcttgttca atcattgatc gaagttcttt atcttggaag aatttgttcc aaagactctg
     7201 aaataaggaa aacaatctat tatatagtct cacacctttg ttttactttt agtgatttca
     7261 atttaataat gtaaatggtt aaaatttatt cttctctgag atcatttcac attgcagata
     7321 gaaaacctga gactggggta atttttatta aaatctaatt taatctcaga aacacatctt
     7381 tattctaaca tcaatttttc cagtttgata ttatcatata aagtcagcct tcctcatctg
     7441 caggttccac aacaaaaatc caaccaactg tggatcaaaa atattgggaa aaaattaaaa
     7501 atagcaatac aacaataaaa aaatacaaat cagaaaaaca gcacagtata acaactttat
     7561 ttagcattta caatctatta ggtattataa gtaatctagc cagatcctct acgccggacg
     7621 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     7681 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     7741 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     7801 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     7861 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
     7921 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7981 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8041 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     8101 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     8161 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8221 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8281 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8341 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8401 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8461 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8521 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8581 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8641 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8701 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8761 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8821 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8881 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8941 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9001 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9061 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9121 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9181 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9241 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9301 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9361 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9421 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9481 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9541 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9601 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9661 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9721 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9781 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9841 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9901 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9961 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10021 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    10081 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
    10141 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    10201 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10261 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    10321 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    10381 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10441 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
    10501 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
    10561 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
    10621 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
    10681 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
    10741 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
    10801 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
    10861 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
    10921 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
    10981 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
    11041 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
    11101 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
    11161 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
    11221 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
    11281 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
    11341 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
    11401 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
    11461 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
    11521 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
    11581 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
    11641 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
    11701 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
    11761 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
    11821 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
    11881 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
    11941 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
    12001 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
    12061 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
    12121 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    12181 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    12241 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    12301 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    12361 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    12421 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
    12481 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
    12541 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
    12601 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
    12661 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
    12721 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
    12781 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
    12841 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
    12901 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
    12961 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
    13021 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
    13081 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
    13141 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
    13201 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
    13261 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
    13321 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
    13381 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
    13441 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
    13501 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
    13561 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
    13621 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
    13681 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
    13741 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
    13801 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
    13861 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
    13921 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
    13981 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
    14041 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
    14101 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
    14161 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    14221 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    14281 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    14341 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    14401 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
    14461 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
    14521 agatccattc gattagtgaa cggatctcga cggtatcgcc gaattcacaa atggcagtat
    14581 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    14641 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    14701 aaaattttcg ggtttattac agggacagca gagatccact
//
