LOCUS       sequence-358019-       14296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian co-expression of human SEPT9_i1 fused to GFP10 and of 
            human SEPT7 Gmut2 fused to GFP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 14296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 ...
FEATURES             Location/Qualifiers
     source          1..14296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1492..1879
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     protein_bind    1573..1591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1609..1627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1644..1662
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1680..1698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1716..1734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1751..1769
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1787..1805
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3952..3969)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4179..4198
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4360..5061
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             5344..5524
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7052..7071)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7285..7304)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7404..7426
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7464..7482)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7550..7654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7655..7723,7724..8515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7873..7892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8686..9274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9175..9194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9428..9445
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9515..9535)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9520..9849
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9700..9835
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10983..11048
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11178..11198
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11623..11757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     11714..11733
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11925..12558
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12605..12730
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13223..13456
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13641..13685
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13978..14095
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    14149..14230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatta aaagatcttc
       61 cctttcttct tgttcttttc caaggttctt gaagagttct gttgttctaa aatacgttgt
      121 tgagcttccc agtttgcttt ctcatcctcg aactgacgac gtttttcctc caattctttg
      181 tgctgtgctt ccaaattctt tttcatttgc tcatggcgcc gctggagctc agcttcagag
      241 tccttcagtt tttgaacttt ttctttgacc ttcatctcaa acacctgctc catctccatc
      301 tccatcttct tcattttagc tacatgctcc cttctttctt cttccatttg tgccagaggg
      361 ctcttagtca gctgcccttt attcttgttg ttatcaactc cattataagt cacagctgca
      421 agttttctgc ttctgtagtt ctcatagtgg acattattag taacatcttt caagtcctgc
      481 atgtgtgttc ttatcaacat atttcttagg attgtaaaat cacaatcttc accattttca
      541 acttcagcaa caccccaagg atactgcctt cctctgaccc ttttgccatt aacttcaatg
      601 atagtattac tacctaccac agcaagaggt aaacggtcct ttatcttttt aacaagttta
      661 ttttcttctt catcatctgt ttctggaaat tcgtatattt taattttatg ttcttggatt
      721 tctttcatta tctgtttttt aaactgttgg cattcctctg gtgtgagtgt gtctgctttg
      781 gcaataagtg ggatgatatt cactttttca tgcaaacgct tcataaactc aatatccaat
      841 ggtttaagtc catgtcctga aggagcaatg aagtataaac aacactgcac cctgttatca
      901 ggcatctgac gtctgttcac tcgtgattct gcatttaggt agtcctcaaa tttactatca
      961 atgtagtcga taacaggctg ccagcaatta ctattatcca ctgcatctcc aaatcctggg
     1021 gtatcaacta ttgtgagcag caactgaaca ccaccttctt tgattaaaac tttggattgt
     1081 tccacctgta cagtcttttt aattctatga gaaggacctg gatactctgg agaatacaaa
     1141 tctgtgagga ataatgagtt gattaatgtc gactttccca atccagattc acccactacc
     1201 ataagcgtga attcaaaacc tctcttcacc gattttctgt atacttgatt tgggagattg
     1261 gcaaatccca catagccttc aaggttcttc tgttgagcta ccatggtgct gctgttgacg
     1321 ctcctctcct cagcagcagc ggatctcgca ctgaccgaca ttccggagga cccaccacct
     1381 ccagagccac cgccaccatc gatggaggcg tcggtgatgc cggcggcggt cacgtactcc
     1441 agcagcacca tgtggtcgcg cttctccatc atatgaccgg tgaattctcc aggcgatctg
     1501 acggttcact aaacgagctc tgcttatata ggcctcccac cgtacacgcc acctcgacat
     1561 actcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     1621 atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
     1681 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     1741 gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     1801 agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
     1861 agtgaaccgt cagatcgcct ggagatttcg agctcggtac cgcggatgaa gaagtcttac
     1921 tcaggaggca cgcggacctc cagtggccgg ctccggaggc ttggtgactc cagtggccca
     1981 gccttaaaaa gatcttttga ggtcgaggag gtcgagacac ccaactccac cccaccccgg
     2041 agggtccaga ctcccctact ccgagccact gtggccagct ccacccagaa attccaggac
     2101 ctgggcgtga agaactcaga accctcggcc cgccatgtgg actccctaag ccaacgctcc
     2161 cccaaggcgt ccctgcggag ggtggagctc tcgggcccca aggcggccga gccggtgtcc
     2221 cggcgcactg agctgtccat tgacatctcg tccaagcagg tggagaacgc cggggccatc
     2281 ggcccgtccc ggttcgggct caagagggcc gaggtgttgg gccacaagac gccagaaccg
     2341 gcccctcgga ggacggagat caccatcgtc aaaccccagg agtcagccca ccggaggatg
     2401 gagccccctg cctccaaggt ccccgaggtg cccactgccc ctgccaccga cgcagccccc
     2461 aagagggtgg agatccagat gcccaagcct gctgaggcgc ccaccgcccc cagcccagcc
     2521 cagaccttgg agaattcaga gcctgcccct gtgtctcagc tgcagagcag gctggagccc
     2581 aagccccagc cccctgtggc tgaggctaca ccccggagcc aggaggccac tgaggcggct
     2641 cccagctgcg ttggcgacat ggccgacacc cccagagatg ccgggctcaa gcaggcgcct
     2701 gcatcacgga acgagaaggc cccggtggac ttcggctacg tggggattga ctccatcctg
     2761 gagcagatgc gccggaaggc catgaagcag ggcttcgagt tcaacatcat ggtggtcggg
     2821 cagagcggct tgggtaaatc caccttaatc aacaccctct tcaaatccaa aatcagccgg
     2881 aagtcggtgc agcccacctc agaggagcgc atccccaaga ccatcgagat caagtccatc
     2941 acgcacgata ttgaggagaa aggcgtccgg atgaagctga cagtgattga cacaccaggg
     3001 ttcggggacc acatcaacaa cgagaactgc tggcagccca tcatgaagtt catcaatgac
     3061 cagtacgaga aatacctgca ggaggaggtc aacatcaacc gcaagaagcg catcccggac
     3121 acccgcgtcc actgctgcct ctacttcatc cccgccaccg gccactccct caggcccctg
     3181 gacatcgagt ttatgaaacg cctgagcaag gtggtcaaca tcgtccctgt catcgccaag
     3241 gcggacacac tcaccctgga ggagagggtc cacttcaaac agcggatcac cgcagacctg
     3301 ctgtccaacg gcatcgacgt gtacccccag aaggaatttg atgaggactc ggaggaccgg
     3361 ctggtgaacg agaagttccg ggagatgatc ccatttgctg tggtgggcag tgaccacgag
     3421 taccaggtca acggcaagag gatccttggg aggaagacca agtggggtac catcgaagtt
     3481 gaaaacacca cacactgtga gtttgcctac ctgcgggacc ttctcatcag gacgcacatg
     3541 cagaacatca aggacatcac cagcagcatc cacttcgagg cgtaccgtgt gaagcgcctc
     3601 aacgagggca gcagcgccat ggccaacggc gtggaggaga aggagccaga agccccagag
     3661 atgatcgatg gtggcggtgg ctctggaggt ggtgggtcct ccggaatggg cgacctgccc
     3721 gacgaccact acctgtccac ccagaccatc ctgagcaagg acctgaacta agctagctaa
     3781 tagttccgcc cctctccctc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     3841 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     3901 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     3961 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4021 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4081 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     4141 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4201 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4261 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     4321 acggggacgt ggttttcctt tgaaaaacac gatgcaagca tgagcgagct gattaaggag
     4381 aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca
     4441 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
     4501 ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag
     4561 accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc
     4621 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     4681 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca
     4741 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg
     4801 taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg
     4861 agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc
     4921 aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac
     4981 gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa
     5041 ctggggcaca agcttaatta aggatccagc ggccgctcga gacctagaaa aacatggagc
     5101 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     5161 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     5221 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5281 ctcccaacga agacaaaatc gtcgagagat gctgcatata agcagctgct ttttgcttgt
     5341 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5401 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5461 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5521 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     5581 tcagagagtg agaggccttg acattataat agatttagca ggaattgaac taggagtgga
     5641 gcacacaggc aaagctgcag aagtacttgg aagaagccac cagagatact cacgattctg
     5701 cacatacctg gctaatccca gatcctaagg attacattaa gtttactaac atttatataa
     5761 tgatttatag tttaaagtat aaacttatct aatttactat tctgacagat attaattaat
     5821 cctcaaatat cataagagat gattactatt atccccattt aacacaagag gaaactgaga
     5881 gggaaagatg ttgaagtaat tttcccacaa ttacagcatc cgttagttac gactctatga
     5941 tcttctgaca caaattccat ttactcctca ccctatgact cagtcgaata tatcaaagtt
     6001 atggacatta tgctaagtaa caaattaccc ttttatatag taaatactga gtagattgag
     6061 agaagaaatt gtttgcaaac ctgaatagct tcaagaagaa gagaagtgag gataagaata
     6121 acagttgtca tttaacaagt tttaacaagt aacttggtta gaaagggatt caaatgcata
     6181 aagcaaggga taaatttttc tggcaacaag actatacaat ataaccttaa atatgacttc
     6241 aaataattgt tggaacttga taaaactaat taaatattat tgaagattat caatattata
     6301 aatgtaattt acttttaaaa agggaacata gaaatgtgta tcattagagt agaaaacaat
     6361 ccttattatc acaatttgtc aaaacaagtt tgttattaac acaagtagaa tactgcattc
     6421 aattaagttg actgcagatt ttgtgttttg ttaaaattag aaagagataa caacaatttg
     6481 aattattgaa agtaacatgt aaatagttct acatacgttc ttttgacatc ttgttcaatc
     6541 attgatcgaa gttctttatc ttggaagaat ttgttccaaa gactctgaaa taaggaaaac
     6601 aatctattat atagtctcac acctttgttt tacttttagt gatttcaatt taataatgta
     6661 aatggttaaa atttattctt ctctgagatc atttcacatt gcagatagaa aacctgagac
     6721 tggggtaatt tttattaaaa tctaatttaa tctcagaaac acatctttat tctaacatca
     6781 atttttccag tttgatatta tcatataaag tcagccttcc tcatctgcag gttccacaac
     6841 aaaaatccaa ccaactgtgg atcaaaaata ttgggaaaaa attaaaaata gcaatacaac
     6901 aataaaaaaa tacaaatcag aaaaacagca cagtataaca actttattta gcatttacaa
     6961 tctattaggt attataagta atctagccag atcctctacg ccggacgcat cgtggccggc
     7021 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     7081 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     7141 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     7201 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     7261 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     7321 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     7381 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     7441 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     7501 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7561 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7621 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7681 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7741 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7801 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7861 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7921 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7981 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8041 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8101 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8161 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8221 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8281 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8341 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8401 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8461 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8521 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8581 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8641 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8701 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8761 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8821 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8881 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8941 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9001 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9061 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9121 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9181 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9241 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9301 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9361 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9421 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9481 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     9541 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9601 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9661 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9721 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9781 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9841 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     9901 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     9961 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
    10021 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
    10081 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
    10141 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
    10201 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
    10261 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
    10321 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
    10381 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
    10441 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
    10501 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
    10561 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
    10621 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
    10681 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
    10741 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
    10801 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
    10861 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
    10921 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
    10981 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
    11041 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
    11101 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
    11161 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
    11221 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
    11281 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
    11341 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
    11401 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
    11461 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
    11521 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    11581 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    11641 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    11701 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    11761 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    11821 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    11881 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
    11941 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
    12001 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
    12061 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
    12121 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
    12181 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
    12241 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
    12301 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
    12361 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    12421 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    12481 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    12541 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
    12601 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
    12661 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
    12721 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
    12781 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
    12841 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
    12901 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
    12961 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
    13021 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
    13081 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
    13141 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    13201 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    13261 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    13321 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    13381 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    13441 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    13501 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    13561 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    13621 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    13681 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    13741 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
    13801 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
    13861 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
    13921 tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt
    13981 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
    14041 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    14101 ttattacagg gacagcagag atccactttg gctgatacgc gtacgtcggc agtgaaaaaa
    14161 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
    14221 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
    14281 ggaggttttt taaagc
//
