LOCUS       sequence-358021-        8927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Gabriel CH, Del Olmo M, Zehtabian A, Jager M, Reischl S, van Dijk H,
            Ulbricht C, Rakhymzhan A, Korte T, Koller B, Grudziecki A, Maier B, 
            Herrmann A, Niesner R, Zemojtel T, Ewers H, Granada AE, Herzel H, 
            Kramer A
  TITLE     Live-cell imaging of circadian clock protein dynamics in 
            CRISPR-generated knock-in cells.
  JOURNAL   Nat Commun. 2021 Jun 18;12(1):3796. doi: 10.1038/s41467-021-24086-9.
  PUBMED    34145278
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-18"; volume: "12(1):3796. doi"; pages: " 
            10.1038/s41467-021-24086-9"
COMMENT     Sequence Label: pDB-hCRY1-mScarlet-I-CD4-bla
FEATURES             Location/Qualifiers
     source          1..8927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(113..1309,1310..1384))
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(1458..1482)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1479..1682)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1508..1528)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2643..2676
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2677..2710)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    2738..2859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2749..2768)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2803..2822
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2871..3266)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(3273..3329)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(3345..4055,4056..4058,4059..4061))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3387..3408)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3736..3755
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3995..4016
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4058..4067
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4117..4447)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4131..4266
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4185..4204)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4432..4452
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     protein_bind    4535..4568
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(4576..4609)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     CDS             complement(4638..4661)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(4671..4688)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(4695..5387)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(4970..4989)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5239..5257
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(5394..5441)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(6331..6350)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6450..6472
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6510..6528)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6596..6700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6701..6769,6770..7561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6919..6938)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7732..8320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8221..8240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8474..8491
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8608..8629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8644..8649,8650..8667,8668..8674)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8682..8698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8687..8709
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8706..8722
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8706..8722
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 acttagtacg gtttcacagg cgtgacacgt ttattgagta ggattacaga gtataacata
       61 gagtataata tagagtatac aatagtgacg tgggatccgc aggtcgcctt cagtgccggc
      121 acctgacaca gaagaagatg cctagcccaa tgaaaagcag gaggccggcg acgcccccca
      181 gcacaatcag ggccattggc tgcaccgggg tcgaccatgt gggcagaacc ttgatgttgg
      241 attccagcag gacctgtccc gagtcactca gcagacactg ccacatcccc gcctcagggt
      301 tcagcaccca caccgccttc tcccgcttcg agacctttgc ctccttgttc tccagtttca
      361 agctcagcat cagcttaggg gaggtgggtc cccacacctc acaggtcaaa tttttctgga
      421 gctgagtggc tctcatcacc accaggttca cttcctgatg caactttcct gttttcgctt
      481 caagggccag ggtgaggttt ccagagccag catactgagg caaggcctgg ggcagggtga
      541 ggtggagcgg gagcttcttg cccatctgga gcttagggtc ctgggtaacc cgttttacag
      601 acacttcctt gttcttcagg tcaaaggtga tccaagactt ggaggaggaa gccctctccg
      661 cctgccacca cagctcgcca ctgcccgtca gcttttcaac tgtaaaggcg agtgggaagg
      721 agaactccac ctgttccccc tctttcttat agactatgct ggaggccttc tggaaagcta
      781 gcaccacgat gtctattttg aactccacct tcttctggtt ctgcaagaca gtgcatgtcc
      841 aggtgccact atcctggagc tccagctgag acacggagag ggtcttcccc ccctgtatgt
      901 ttttacccct tggactccta cattgcactg aggggctact accagggggg ctctccaagg
      961 tcagggtcag gctctgcccc tgaagcaggt gggtgtcaga gttggcagtc aatccgaaca
     1021 ctagcaattg cacctcctcc ttctggtcct ccacttcaca gatgtaagta tctgagtctt
     1081 ctatcttaag attcttgatg atcaggggga agtttccttg gtcccaaagg cttcttcttg
     1141 agtcagcgcg atcattcagc ttggatggac ctttagttaa gaaggagccc tgatttccca
     1201 gaatctttat ctggttggag tttttccagt ggaattgtat gctcttcttc tgggaagctg
     1261 tacaggtcag ttccactgta tccccttttt tgcccagcac cactttcttt ccctgagtgg
     1321 ctgctgggag gagcgccagt tgcagcacca gaagcaagtg cctaaaaggg actccccggt
     1381 tcatggtgga tccccaggct ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt
     1441 ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat atagacctcc
     1501 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
     1561 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
     1621 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
     1681 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     1741 gtcatgtact gggcataatg ccagggaatt gacctgcagg catgcaagct tgtcgaccag
     1801 tattcctcct gaatgttttc tgtgatttaa gggggaaaaa aaaactaggt aaagcttgat
     1861 tgcaaataat tcaatgacaa aaatgtgatg agtgtttatt gaccttatct tcaagatact
     1921 tatagtctaa ttaggttgac ataaaaaaac taagcaataa aaaatgtaaa cagtgttaga
     1981 aactgaagaa acagaaaatt tttaaaaact ggcaaatcca acgtataaat atttttcata
     2041 ttaatttaaa tcacaaaatg acatcccaaa tccaattaaa ttgaatgcag gcaaggcttt
     2101 ctgatacttc taataagggc cagcatttta aaaaattttg aactattccc aaattggaaa
     2161 taatttagat tttagaagca tcataacaga taaaagaaat ttatcaacat ttaagaggca
     2221 aagatttagg ggaaagttca taaaaagatt atattaatgt cactaaaata aacaggccac
     2281 tttaattgtt ttagactcat agttacatag taaggaaata aaaaagttgt ctttttctaa
     2341 gaaaaaactt gaccacaaaa tcataaacat ctctaattta aagagcattg gttcagatga
     2401 atataaaact ggtgcaacaa tttctccagt acagatgcat gtctcttgac caaaaattaa
     2461 ctgagtttgt aaaaattgct taaaaattaa tgcctcaaga ttttctggtt tgaaaataga
     2521 gatttgctcc atttttgaaa ttatcttaat ttatgttaat tatatactct tctaaagcaa
     2581 gaaatacagc tctaaaatat ttacctaatt agtgcattcg aagcgatcgc ttaattaatc
     2641 aaataacttc gtataatgta tgctatacga agttatgaag ttcctatact atttgaagaa
     2701 taggaacttc ggaataggaa cttctataca gacatgataa gatacattga tgagtttgga
     2761 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     2821 gctttatttg taaccattat aagctgcaat aaacaagttg gggtctcgag ttagccctcc
     2881 cacacataac cagagggcag caattcacga atcccaactg ccgtcggctg tccatcactg
     2941 tccttcacta tggctttgat cccaggatgc agatcgagaa gcacctgtcg gcaccgtccg
     3001 caggggctca agatgcccct gttctcattt ccgatcgcga cgatacaagt caggttgcca
     3061 gctgccgcag cagcagcagt gcccagcacc acgagttctg cacaaggtcc cccagtaaaa
     3121 tgatatacat tgacaccagt gaagatgcgg ccgtcgctag agagagctgc gctggcgacg
     3181 ctgtagtctt cagagatggg gatgctgttg attgtagccg ttgctctttc aatgagggtg
     3241 gattcttctt gagacaaagg cttggccacg tgaggtccag ggttctcctc cacgtctcca
     3301 gcctgcttca gcaggctgaa gttagtagct ccgcttccga attccttgta cagctcgtcc
     3361 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     3421 tcgttggggt ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc
     3481 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     3541 tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg
     3601 gtgatataga cgttgtcgct gatggcgttg tactccagct tgtgccccag gatgttgccg
     3661 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     3721 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
     3781 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     3841 cgggcgaagc actgcacgcc ccaggtcagg gtggtcacga gggtgggcca gggcacgggc
     3901 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
     3961 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     4021 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcggt acctgctcag
     4081 tgctgatcag atccgaaaat ggatatacaa tagctttttg caaaagccta ggcctccaaa
     4141 aaagcctcct cactacttct ggaatagctc agaggcagag gcggcctcgg cctctgcata
     4201 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     4261 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     4321 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     4381 atgcatgctt tgcatacttc tgcctgcctg gggagcctgg ggactttcca caccctaact
     4441 gacacacatt ccacagcgtt aaatttttgt taaatcagct caacacaaaa aaccaacaca
     4501 cagatgtaat gaaaataaag atattttatt caaaataact tcgtataatg tatgctatac
     4561 gaagttatta gatctgaagt tcctatacta tttgaagaat aggaacttcg gaataggaac
     4621 ttcagatctt tgctagcctt gtcgtcatcg tctttgtagt caccgctacc atggtgatgg
     4681 tgatggtgac tagtcttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggag
     4741 cgttcgtact gttccaccac ggtgtagtcc tcgttgtggg aggtgatgtc caacttgcgg
     4801 tcgacgttgt aggcgccggg catctgcacg ggcttcttgg ccttgtaggt ggtcttgaag
     4861 tccgccaggt agcggccgcc gtccttcagg cgcagggcca tcttaatgtc gcccttcagc
     4921 acgccgtcct cggggtacaa ccgctcggtg gacgcttccc agcccattgt cttcttctgc
     4981 attacggggc cgtcaggagg gaagttggtg ccgcggagct tcaccttgta gatcagggtg
     5041 ccgtcctcca gggaggtgtc ctgggtcacg gtcacggcgc cgccgtcctc gaagttcatc
     5101 acgcgctccc acttgaagcc ctcggggaag gactgcttat agtagtcggg gatgtcggcg
     5161 gggtgcttga tgaaggccct ggagccgtac atgaactgag gggacaggat gtcccaggag
     5221 aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
     5281 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcatggagcc ctccatgtgc
     5341 accttgaacc gcatgaactc cttgatcact gcctcgccct tgctcacacc ggtgaagttc
     5401 ctatactttc tagagaatag gaacttcgga ataggaactt cgcggccgct attagtgctc
     5461 tgtctctgga ctttaggacc aatactctgt gtgtcttctt cttgactagg acgtttccca
     5521 ccactgagac cagtgcccat ggagcttctt cctgcacatt taaaaaatgt atgtttaaga
     5581 taggaaaagg aaaaagaaca gtatgctcca atgcttcatt cttaccttgc ttcaacaggt
     5641 gagtttgctg actgtcgcca tgagcatagt gtaaaatacc actcccttga gagcaacctg
     5701 ttagtattta aaacacagta aaattactta aaatcaaatc caagatgtat gtattattaa
     5761 gtctacaaat ccaactttta gcgcttgtac ttaaggtcat ttatatgctt ttaagactaa
     5821 atctcaatct tcctatgaac caaaaatcac atgctcaaac caggaaaact taaatttagg
     5881 ctcacagaag tgttgacagc tcaaaggaaa aatcattgat gatcattttc taaaaaacat
     5941 ttccttacta gatgacaatt tgaaacttga ccatgaaggt aaaacatagg ggcaagagtg
     6001 agatagacat tcctccctca ctcttgtccc ttttcccacg cttttccttc agagctcctc
     6061 caaacatatt tccttgagga taagggaata agggcctgcc cgatgagtta aacaattcaa
     6121 atcatacgac aagcagaaga cacagactat gtggcagtta cctagcctaa gcaatcctat
     6181 caacccttct aaaaacaaac actattatag gagaataggt tggctagaac agagaagtat
     6241 gttttaggga aacatttctg tcagtgtgtc cagtttatgg aaaaatatga gtttaaacct
     6301 taagggccgg cccatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6361 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     6421 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     6481 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     6541 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     6601 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6661 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6721 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6781 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6841 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6901 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6961 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7021 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7081 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7141 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7201 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7261 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7321 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7381 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7441 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7501 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7561 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7621 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7681 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7741 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7801 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7861 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7921 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7981 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8041 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8101 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8161 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8221 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8281 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8341 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8401 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8461 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8521 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8581 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8641 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8701 tcacacagga aacagctatg accatgatta cgccaagctt gggctgcagg tcggggagag
     8761 gagtgttagt aaccgcgacg ctggtggggg tcggcttgtt aagaggggcg ctgctaacgc
     8821 tgcaagagtg ggttgtcagc gtggggccgg tgctactgga atcgataccg gcatgattga
     8881 cagcctgggc gaggatgtca cctgatggtg ataagaagac acgggag
//
