LOCUS       sequence-358033-        6395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human SEPT9_i1 DN7 fused to monomeric 
            superfolder GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Kuzmic M, Linares GC, Fialova JL, Iv F, Salaun D, Llewellyn A, Gomes
            M, Belhabib M, Liu Y, Asano K, Rodrigues M, Isnardon D, Tachibana T,
            Koenderink GH, Badache A, Mavrakis M, Verdier-Pinard P
  TITLE     Septin-microtubule association via a motif unique to the isoform 1 
            of septin 9 tunes stress fibers.
  JOURNAL   J Cell Sci. 2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850.
  PUBMED    34854883
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850."
COMMENT     Sequence Label: pCMV_SEPT9_i1 DN7-msfGFP
FEATURES             Location/Qualifiers
     source          1..6395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3183..3304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3220..3239)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3274..3293
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3311..3766)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3448..3469)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3660..3679
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3793..3897
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3899..4256
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3899..4090
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3922..3942)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4107..4242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4291..5085
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4291..5085
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4345..4364)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4955..4974
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5273..5292)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5317..5364
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5693..6281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6182..6201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
      601 gcacgcggac ctccagtggc cggctccgga ggcttggtga ctccagtggc ccagccttaa
      661 aaagatcttt tgaggtcgag gaggtcgaga cacccaactc caccccaccc cggagggtcc
      721 agactcccct actccgagcc actgtggcca gctccaccca gaaattccag gacctgggcg
      781 tgaagaactc agaaccctcg gcccgccatg tggactccct aagccaacgc tcccccaagg
      841 cgtccctgcg gagggtggag ctctcgggcc ccaaggcggc cgagccggtg tcccggcgca
      901 ctgagctgtc cattgacatc tcgtccaagc aggtggagaa cgccggggcc atcggcccgt
      961 cccggttcgg gctcaagagg gccgaggtgt tgggccacaa gacgccagaa ccggcccctc
     1021 ggaggacgga gatcaccatc gtcaaacccc aggagtcagc ccaccggagg atggagcccc
     1081 ctgcctccaa ggtccccgag gtgcccactg cccctgccac cgacgcagcc cccaagaggg
     1141 tggagatcca gatgcccaag cctgctgagg cgcccaccgc ccccagccca gcccagacct
     1201 tggagaattc agagcctgcc cctgtgtctc agctgcagag caggctggag cccaagcccc
     1261 agccccctgt ggctgaggct acaccccgga gccaggaggc cactgaggcg gctcccagct
     1321 gcgttggcga catggccgac acccccagag atgccgggct caagcaggcg cctgcatcac
     1381 ggaacgagaa ggccccggtg gacttcggct acgtggggat tgactccatc ctggagcaga
     1441 tgcgccggaa ggccatgaag cagggcttcg agttcaacat catggtggtc gggcagagcg
     1501 gcttgggtaa atccacctta atcaacaccc tcttcaaatc caaaatcagc cggaagtcgg
     1561 tgcagcccac ctcagaggag cgcatcccca agaccatcga gatcaagtcc atcacgcacg
     1621 atattgagga gaaaggcgtc cggatgaagc tgacagtgat tgacacacca gggttcgggg
     1681 accacatcaa caacgagaac tgctggcagc ccatcatgaa gttcatcaat gaccagtacg
     1741 agaaatacct gcaggaggag gtcaacatca accgcaagaa gcgcatcccg gacacccgcg
     1801 tccactgctg cctctacttc atccccgcca ccggccactc cctcaggccc ctggacatcg
     1861 agtttatgaa acgcctgagc aaggtggtca acatcgtccc tgtcatcgcc aaggcggaca
     1921 cactcaccct ggaggagagg gtccacttca aacagcggat caccgcagac ctgctgtcca
     1981 acggcatcga cgtgtacccc cagaaggaat ttgatgagga ctcggaggac cggctggtga
     2041 acgagaagtt ccgggagatg atcccatttg ctgtggtggg cagtgaccac gagtaccagg
     2101 tcaacggcaa gaggatcctt gggaggaaga ccaagtgggg taccatcgaa gttgaaaaca
     2161 ccacacactg tgagtttgcc tacctgcggg accttctcat caggacgcac atgcagaaca
     2221 tcaaggacat caccagcagc atccacttcg aggcgtaccg tgtgaagcgc ctcaacgagg
     2281 gcagcagcgc catggccaac ggcgtggagg agaaggagcc agaagcccca gagatggtgt
     2341 ccaagggcga ggagctgttc accggcgtgg tgcccatcct ggtggagctg gatggcgacg
     2401 tgaacggcca caagttcagc gtgcgcggcg agggcgaggg cgacgccacc aacggcaagc
     2461 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctggtga
     2521 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgatcacatg aagcagcacg
     2581 atttcttcaa gagcgccatg cccgagggct acgtgcagga gcgcaccatc agcttcaagg
     2641 atgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgatacc ctggtgaacc
     2701 gcatcgagct gaagggcatc gatttcaagg aggatggcaa catcctgggc cacaagctgg
     2761 agtacaactt caacagccac aacgtgtaca tcaccgccga taagcagaag aacggcatca
     2821 aggccaactt caagatccgc cacaatgtgg aggatggctc cgtgcagctg gccgatcact
     2881 accagcagaa cacccccatc ggcgacggcc cagtgctgct gcccgataac cactacctga
     2941 gcacccagag caagctgtcc aaggacccca acgagaagcg cgatcacatg gtgctgctgg
     3001 agttcgtgac cgccgccggc atcaccctgg gcatggatga gctgtacaag taaggatcca
     3061 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     3121 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     3181 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3241 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3301 cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3361 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3421 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3481 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3541 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3601 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3661 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3721 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     3781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3841 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     3901 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3961 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4021 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4081 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4141 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     4201 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     4261 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     4321 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     4381 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     4441 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     4501 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4561 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4621 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4681 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     4741 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     4801 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     4861 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     4921 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     4981 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5041 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     5101 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5161 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5221 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     5281 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     5341 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     5401 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     5461 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     5521 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     5581 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5641 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5701 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5761 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5821 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5881 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5941 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6001 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6061 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6121 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6181 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6241 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6301 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6361 ctgattctgt ggataaccgt attaccgcca tgcat
//
