LOCUS       sequence-358034-        6365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human SEPT9_i1 D10-25 fused to monomeric 
            superfolder GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Kuzmic M, Linares GC, Fialova JL, Iv F, Salaun D, Llewellyn A, Gomes
            M, Belhabib M, Liu Y, Asano K, Rodrigues M, Isnardon D, Tachibana T,
            Koenderink GH, Badache A, Mavrakis M, Verdier-Pinard P
  TITLE     Septin-microtubule association via a motif unique to the isoform 1 
            of septin 9 tunes stress fibers.
  JOURNAL   J Cell Sci. 2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850.
  PUBMED    34854883
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850."
COMMENT     Sequence Label: pCMV_SEPT9_i1 D10-25-msfGFP
FEATURES             Location/Qualifiers
     source          1..6365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3153..3274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3190..3209)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3244..3263
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3281..3736)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3418..3439)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3630..3649
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3763..3867
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3869..4226
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3869..4060
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3892..3912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4077..4212
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4261..5055
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4261..5055
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4315..4334)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4925..4944
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5243..5262)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5287..5334
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5663..6251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6152..6171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatga
      601 agaagtctta ctcaggaggc acggccttga aaagatcttt tgaggtcgag gaggtcgaga
      661 cacccaactc caccccaccc cggagggtcc agactcccct actccgagcc actgtggcca
      721 gctccaccca gaaattccag gacctgggcg tgaagaactc agaaccctcg gcccgccatg
      781 tggactccct aagccaacgc tcccccaagg cgtccctgcg gagggtggag ctctcgggcc
      841 ccaaggcggc cgagccggtg tcccggcgca ctgagctgtc cattgacatc tcgtccaagc
      901 aggtggagaa cgccggggcc atcggcccgt cccggttcgg gctcaagagg gccgaggtgt
      961 tgggccacaa gacgccagaa ccggcccctc ggaggacgga gatcaccatc gtcaaacccc
     1021 aggagtcagc ccaccggagg atggagcccc ctgcctccaa ggtccccgag gtgcccactg
     1081 cccctgccac cgacgcagcc cccaagaggg tggagatcca gatgcccaag cctgctgagg
     1141 cgcccaccgc ccccagccca gcccagacct tggagaattc agagcctgcc cctgtgtctc
     1201 agctgcagag caggctggag cccaagcccc agccccctgt ggctgaggct acaccccgga
     1261 gccaggaggc cactgaggcg gctcccagct gcgttggcga catggccgac acccccagag
     1321 atgccgggct caagcaggcg cctgcatcac ggaacgagaa ggccccggtg gacttcggct
     1381 acgtggggat tgactccatc ctggagcaga tgcgccggaa ggccatgaag cagggcttcg
     1441 agttcaacat catggtggtc gggcagagcg gcttgggtaa atccacctta atcaacaccc
     1501 tcttcaaatc caaaatcagc cggaagtcgg tgcagcccac ctcagaggag cgcatcccca
     1561 agaccatcga gatcaagtcc atcacgcacg atattgagga gaaaggcgtc cggatgaagc
     1621 tgacagtgat tgacacacca gggttcgggg accacatcaa caacgagaac tgctggcagc
     1681 ccatcatgaa gttcatcaat gaccagtacg agaaatacct gcaggaggag gtcaacatca
     1741 accgcaagaa gcgcatcccg gacacccgcg tccactgctg cctctacttc atccccgcca
     1801 ccggccactc cctcaggccc ctggacatcg agtttatgaa acgcctgagc aaggtggtca
     1861 acatcgtccc tgtcatcgcc aaggcggaca cactcaccct ggaggagagg gtccacttca
     1921 aacagcggat caccgcagac ctgctgtcca acggcatcga cgtgtacccc cagaaggaat
     1981 ttgatgagga ctcggaggac cggctggtga acgagaagtt ccgggagatg atcccatttg
     2041 ctgtggtggg cagtgaccac gagtaccagg tcaacggcaa gaggatcctt gggaggaaga
     2101 ccaagtgggg taccatcgaa gttgaaaaca ccacacactg tgagtttgcc tacctgcggg
     2161 accttctcat caggacgcac atgcagaaca tcaaggacat caccagcagc atccacttcg
     2221 aggcgtaccg tgtgaagcgc ctcaacgagg gcagcagcgc catggccaac ggcgtggagg
     2281 agaaggagcc agaagcccca gagatggtgt ccaagggcga ggagctgttc accggcgtgg
     2341 tgcccatcct ggtggagctg gatggcgacg tgaacggcca caagttcagc gtgcgcggcg
     2401 agggcgaggg cgacgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca
     2461 agctgcccgt gccctggccc accctggtga ccaccctgac ctacggcgtg cagtgcttca
     2521 gccgctaccc cgatcacatg aagcagcacg atttcttcaa gagcgccatg cccgagggct
     2581 acgtgcagga gcgcaccatc agcttcaagg atgacggcac ctacaagacc cgcgccgagg
     2641 tgaagttcga gggcgatacc ctggtgaacc gcatcgagct gaagggcatc gatttcaagg
     2701 aggatggcaa catcctgggc cacaagctgg agtacaactt caacagccac aacgtgtaca
     2761 tcaccgccga taagcagaag aacggcatca aggccaactt caagatccgc cacaatgtgg
     2821 aggatggctc cgtgcagctg gccgatcact accagcagaa cacccccatc ggcgacggcc
     2881 cagtgctgct gcccgataac cactacctga gcacccagag caagctgtcc aaggacccca
     2941 acgagaagcg cgatcacatg gtgctgctgg agttcgtgac cgccgccggc atcaccctgg
     3001 gcatggatga gctgtacaag taaggatcca ccggatctag ataactgatc ataatcagcc
     3061 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     3121 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     3181 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3241 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt
     3301 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     3361 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     3421 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3481 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3541 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3601 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3661 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3721 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3841 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     3901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4081 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4141 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     4201 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     4261 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4321 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4381 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     4441 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     4501 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4561 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     4621 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     4681 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     4741 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     4801 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     4861 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     4921 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     4981 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5041 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     5101 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5161 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5221 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     5281 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     5341 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     5401 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     5461 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     5521 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5581 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     6361 tgcat
//
