LOCUS       sequence-358037-        5732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human SEPT9_i1-i5 fused to monomeric 
            superfolder GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Kuzmic M, Linares GC, Fialova JL, Iv F, Salaun D, Llewellyn A, Gomes
            M, Belhabib M, Liu Y, Asano K, Rodrigues M, Isnardon D, Tachibana T,
            Koenderink GH, Badache A, Mavrakis M, Verdier-Pinard P
  TITLE     Septin-microtubule association via a motif unique to the isoform 1 
            of septin 9 tunes stress fibers.
  JOURNAL   J Cell Sci. 2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850.
  PUBMED    34854883
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850."
COMMENT     Sequence Label: pCMV_SEPT9_i1-i5-msfGFP
FEATURES             Location/Qualifiers
     source          1..5732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2520..2641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2557..2576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2611..2630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2648..3103)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2785..2806)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2997..3016
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3130..3234
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3236..3593
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3236..3427
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3259..3279)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3444..3579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3628..4422
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3628..4422
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3682..3701)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4292..4311
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4610..4629)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4654..4701
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5030..5618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5519..5538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatga
      601 agaagtctta ctcaggaggc acgcggacct ccagtggccg gctccggagg cttggtgact
      661 ccagtggccc agccgacacc cccagagatg ccgggctcaa gcaggcgcct gcatcacgga
      721 acgagaaggc cccggtggac ttcggctacg tggggattga ctccatcctg gagcagatgc
      781 gccggaaggc catgaagcag ggcttcgagt tcaacatcat ggtggtcggg cagagcggct
      841 tgggtaaatc caccttaatc aacaccctct tcaaatccaa aatcagccgg aagtcggtgc
      901 agcccacctc agaggagcgc atccccaaga ccatcgagat caagtccatc acgcacgata
      961 ttgaggagaa aggcgtccgg atgaagctga cagtgattga cacaccaggg ttcggggacc
     1021 acatcaacaa cgagaactgc tggcagccca tcatgaagtt catcaatgac cagtacgaga
     1081 aatacctgca ggaggaggtc aacatcaacc gcaagaagcg catcccggac acccgcgtcc
     1141 actgctgcct ctacttcatc cccgccaccg gccactccct caggcccctg gacatcgagt
     1201 ttatgaaacg cctgagcaag gtggtcaaca tcgtccctgt catcgccaag gcggacacac
     1261 tcaccctgga ggagagggtc cacttcaaac agcggatcac cgcagacctg ctgtccaacg
     1321 gcatcgacgt gtacccccag aaggaatttg atgaggactc ggaggaccgg ctggtgaacg
     1381 agaagttccg ggagatgatc ccatttgctg tggtgggcag tgaccacgag taccaggtca
     1441 acggcaagag gatccttggg aggaagacca agtggggtac catcgaagtt gaaaacacca
     1501 cacactgtga gtttgcctac ctgcgggacc ttctcatcag gacgcacatg cagaacatca
     1561 aggacatcac cagcagcatc cacttcgagg cgtaccgtgt gaagcgcctc aacgagggca
     1621 gcagcgccat ggccaacggc gtggaggaga aggagccaga agccccagag atggtgtcca
     1681 agggcgagga gctgttcacc ggcgtggtgc ccatcctggt ggagctggat ggcgacgtga
     1741 acggccacaa gttcagcgtg cgcggcgagg gcgagggcga cgccaccaac ggcaagctga
     1801 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctggtgacca
     1861 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga tcacatgaag cagcacgatt
     1921 tcttcaagag cgccatgccc gagggctacg tgcaggagcg caccatcagc ttcaaggatg
     1981 acggcaccta caagacccgc gccgaggtga agttcgaggg cgataccctg gtgaaccgca
     2041 tcgagctgaa gggcatcgat ttcaaggagg atggcaacat cctgggccac aagctggagt
     2101 acaacttcaa cagccacaac gtgtacatca ccgccgataa gcagaagaac ggcatcaagg
     2161 ccaacttcaa gatccgccac aatgtggagg atggctccgt gcagctggcc gatcactacc
     2221 agcagaacac ccccatcggc gacggcccag tgctgctgcc cgataaccac tacctgagca
     2281 cccagagcaa gctgtccaag gaccccaacg agaagcgcga tcacatggtg ctgctggagt
     2341 tcgtgaccgc cgccggcatc accctgggca tggatgagct gtacaagtaa ggatccaccg
     2401 gatctagata actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     2461 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     2521 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     2581 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     2641 aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     2701 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     2761 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     2821 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     2881 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     2941 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     3001 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     3061 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     3121 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3181 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
     3241 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     3301 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     3361 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3421 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3481 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     3541 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
     3601 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     3661 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     3721 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     3781 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     3841 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     3901 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     3961 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     4021 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     4081 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     4141 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     4201 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     4261 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     4321 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     4381 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     4441 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     4501 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     4561 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     4621 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     4681 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     4741 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     4801 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     4861 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4921 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4981 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5041 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5101 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5161 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5221 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5281 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5341 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5401 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5461 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5521 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5581 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5641 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5701 attctgtgga taaccgtatt accgccatgc at
//
