LOCUS       sequence-358041-        5966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human SEPT7 Gmut2 fused to monomeric 
            superfolder GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pCMV_msfGFP-SEPT7 Gmut2
FEATURES             Location/Qualifiers
     source          1..5966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2754..2875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2791..2810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2845..2864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2882..3337)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3019..3040)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3231..3250
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3364..3468
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3470..3827
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3470..3661
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3493..3513)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3678..3813
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3862..4656
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3862..4656
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3916..3935)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4526..4545
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4844..4863)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4888..4935
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5264..5852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5753..5772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
      601 tgtccaaggg cgaggagctg ttcaccggcg tggtgcccat cctggtggag ctggatggcg
      661 acgtgaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgacgcc accaacggca
      721 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctgg
      781 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgatcac atgaagcagc
      841 acgatttctt caagagcgcc atgcccgagg gctacgtgca ggagcgcacc atcagcttca
      901 aggatgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgat accctggtga
      961 accgcatcga gctgaagggc atcgatttca aggaggatgg caacatcctg ggccacaagc
     1021 tggagtacaa cttcaacagc cacaacgtgt acatcaccgc cgataagcag aagaacggca
     1081 tcaaggccaa cttcaagatc cgccacaatg tggaggatgg ctccgtgcag ctggccgatc
     1141 actaccagca gaacaccccc atcggcgacg gcccagtgct gctgcccgat aaccactacc
     1201 tgagcaccca gagcaagctg tccaaggacc ccaacgagaa gcgcgatcac atggtgctgc
     1261 tggagttcgt gaccgccgcc ggcatcaccc tgggcatgga tgagctgtac aagtcggtca
     1321 gtgcgagatc cgctgctgct gaggagagga gcgtcaacag cagcaccatg gtagctcaac
     1381 agaagaacct tgaaggctat gtgggatttg ccaatctccc aaatcaagta tacagaaaat
     1441 cggtgaagag aggttttgaa ttcacgctta tggtagtggg tgaatctgga ttgggaaagt
     1501 cgacattaat caactcatta ttcctcacag atttgtattc tccagagtat ccaggtcctt
     1561 ctcatagaat taaaaagact gtacaggtgg aacaatccaa agttttaatc aaagaaggtg
     1621 gtgttcagtt gctgctcaca atagttgata ccccaggatt tggagatgca gtggataata
     1681 gtaattgctg gcagcctgtt atcgactaca ttgatagtaa atttgaggac tacctaaatg
     1741 cagaatcacg agtgaacaga cgtcagatgc ctgataacag ggtgcagtgt tgtttatact
     1801 tcattgctcc ttcaggacat ggacttaaac cattggatat tgagtttatg aagcgtttgc
     1861 atgaaaaagt gaatatcatc ccacttattg ccaaagcaga cacactcaca ccagaggaat
     1921 gccaacagtt taaaaaacag ataatgaaag aaatccaaga acataaaatt aaaatatacg
     1981 aatttccaga aacagatgat gaagaagaaa ataaacttgt taaaaagata aaggaccgtt
     2041 tacctcttgc tgtggtaggt agtaatacta tcattgaagt taatggcaaa agggtcagag
     2101 gaaggcagta tccttggggt gttgctgaag ttgaaaatgg tgaagattgt gattttacaa
     2161 tcctaagaaa tatgttgata agaacacaca tgcaggactt gaaagatgtt actaataatg
     2221 tccactatga gaactacaga agcagaaaac ttgcagctgt gacttataat ggagttgata
     2281 acaacaagaa taaagggcag ctgactaaga gccctctggc acaaatggaa gaagaaagaa
     2341 gggagcatgt agctaaaatg aagaagatgg agatggagat ggagcaggtg tttgagatga
     2401 aggtcaaaga aaaagttcaa aaactgaagg actctgaagc tgagctccag cggcgccatg
     2461 agcaaatgaa aaagaatttg gaagcacagc acaaagaatt ggaggaaaaa cgtcgtcagt
     2521 tcgaggatga gaaagcaaac tgggaagctc aacaacgtat tttagaacaa cagaactctt
     2581 caagaacctt ggaaaagaac aagaagaaag ggaagatctt ttaaggatcc accggatcta
     2641 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2701 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     2881 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2941 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     3001 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     3061 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     3121 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     3181 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3241 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3301 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     3361 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3421 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     3481 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3541 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3601 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3661 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3721 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     3781 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     3841 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     3901 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     3961 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4021 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     4081 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4141 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4201 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4261 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4321 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4381 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4441 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4501 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     4561 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4621 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     4681 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     4741 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     4801 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     4861 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     4921 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     4981 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     5041 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     5101 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5161 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5401 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5641 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5821 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5881 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5941 tggataaccg tattaccgcc atgcat
//
