LOCUS       sequence-358043-        6359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human SEPT9_i3 NCmut fused to monomeric 
            superfolder GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pCMV_SEPT9_i3 NCmut-msfGFP
FEATURES             Location/Qualifiers
     source          1..6359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3147..3268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3184..3203)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3238..3257
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3275..3730)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3412..3433)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3624..3643
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3757..3861
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3863..4220
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3863..4054
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3886..3906)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4071..4206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4255..5049
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4255..5049
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4309..4328)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4919..4938
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5237..5256)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5281..5328
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5657..6245
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6146..6165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
      601 agagggaccg gatctcagcc ttgaaaagat cttttgaggt cgaggaggtc gagacaccca
      661 actccacccc accccggagg gtccagactc ccctactccg agccactgtg gccagctcca
      721 cccagaaatt ccaggacctg ggcgtgaaga actcagaacc ctcggcccgc catgtggact
      781 ccctaagcca acgctccccc aaggcgtccc tgcggagggt ggagctctcg ggccccaagg
      841 cggccgagcc ggtgtcccgg cgcactgagc tgtccattga catctcgtcc aagcaggtgg
      901 agaacgccgg ggccatcggc ccgtcccggt tcgggctcaa gagggccgag gtgttgggcc
      961 acaagacgcc agaaccggcc cctcggagga cggagatcac catcgtcaaa ccccaggagt
     1021 cagcccaccg gaggatggag ccccctgcct ccaaggtccc cgaggtgccc actgcccctg
     1081 ccaccgacgc agcccccaag agggtggaga tccagatgcc caagcctgct gaggcgccca
     1141 ccgcccccag cccagcccag accttggaaa attcagagcc tgcccctgtg tctcagctgc
     1201 agagcaggct ggagcccaag ccccagcccc ctgtggctga ggctacaccc cggagccagg
     1261 aggccactga ggcggctccc agctgcgttg gcgacatggc cgacaccccc agagatgccg
     1321 ggctcaagca ggcgcctgca tcacggaacg agaaggcccc ggtggacttc ggctacgtgg
     1381 gggatgactc catcctggag caggatcgcc ggaaggccat gaagcagggc ttcgagttca
     1441 acatcatggt ggtcgggcag agcggcttgg gtaaatccac cttaatcaac accctcttca
     1501 aatccaaaat cagccggaag tcggtgcagc ccacctcaga ggagcgcatc cccaagacca
     1561 tcgagatcaa gtccatcacg cacgatattg aggagaaagg cgtccggatg aagctgacag
     1621 tgattgacac accagggttc ggggaccaca tcaacaacga gaactgctgg cagcccatca
     1681 tgaagttcat caatgaccag tacgagaaat acctgcagga ggaggtcaac atcaaccgca
     1741 agaagcgcat cccggacacc cgcgtccact gctgcctcta cttcatcccc gccaccggcc
     1801 actccctcag gcccctggac atcgagttta tgaaacgcct gagcaaggtg gtcaacatcg
     1861 tccctgtcat cgccaaggcg gacacactca ccctggagga gagggtccac ttcaaacagc
     1921 ggatcaccgc agacctgctg tccaacggca tcgacgtgta cccccagaag gaatttgatg
     1981 aggactcgga ggaccggctg gtgaacgaga agttccggga gatgatccca tttgctgtgg
     2041 tgggcagtga ccacgagtac caggtcaacg gcaagaggat ccttgggagg aagaccaagt
     2101 ggggtaccat cgaagttgaa aacaccacac actgtgagtt tgcctacctg cgggaccttc
     2161 tcatcaggac gcacatgcag aacatcaagg acatcaccag cagcatccac ttcgaggcgt
     2221 accgtgtgaa gcgcctcaac gagggcagca gcgccatggc caacggcgtg gaggagaagg
     2281 agccagaagc cccagagatg gtgtccaagg gcgaggagct gttcaccggc gtggtgccca
     2341 tcctggtgga gctggatggc gacgtgaacg gccacaagtt cagcgtgcgc ggcgagggcg
     2401 agggcgacgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     2461 ccgtgccctg gcccaccctg gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     2521 accccgatca catgaagcag cacgatttct tcaagagcgc catgcccgag ggctacgtgc
     2581 aggagcgcac catcagcttc aaggatgacg gcacctacaa gacccgcgcc gaggtgaagt
     2641 tcgagggcga taccctggtg aaccgcatcg agctgaaggg catcgatttc aaggaggatg
     2701 gcaacatcct gggccacaag ctggagtaca acttcaacag ccacaacgtg tacatcaccg
     2761 ccgataagca gaagaacggc atcaaggcca acttcaagat ccgccacaat gtggaggatg
     2821 gctccgtgca gctggccgat cactaccagc agaacacccc catcggcgac ggcccagtgc
     2881 tgctgcccga taaccactac ctgagcaccc agagcaagct gtccaaggac cccaacgaga
     2941 agcgcgatca catggtgctg ctggagttcg tgaccgccgc cggcatcacc ctgggcatgg
     3001 atgagctgta caagtgagga tccaccggat ctagataact gatcataatc agccatacca
     3061 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3121 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3181 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3241 gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta
     3301 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3361 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3421 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3481 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3541 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3601 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3661 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3721 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3781 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3841 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     3901 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3961 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4021 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4081 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4141 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4201 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4261 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4321 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4381 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     4441 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4501 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4561 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4621 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4681 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4741 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     4801 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4861 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4921 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     4981 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5041 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5101 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5161 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5221 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     5281 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     5341 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     5401 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     5461 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     5521 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5581 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5641 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5701 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5761 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5821 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5881 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5941 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6001 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6061 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6121 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6181 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6241 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6301 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
