LOCUS       sequence-358053-        5738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human SEPT2 NCmut fused to monomeric 
            superfolder GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pCMV_SEPT2 NCmut-msfGFP
FEATURES             Location/Qualifiers
     source          1..5738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2526..2647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2563..2582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2617..2636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2654..3109)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2791..2812)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3003..3022
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3136..3240
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3242..3599
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3242..3433
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3265..3285)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3450..3585
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3634..4428
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3634..4428
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3688..3707)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4298..4317
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4616..4635)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4660..4707
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5036..5624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5525..5544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgt
      601 ctaagcaaca accaactcag tttataaatc cagaaactcc tggctatgtt ggagatgcaa
      661 atcttcccaa tcaagatcac cgaaaatcag tgaagaaggg gttcgagttc actctgatgg
      721 tggttggtga atctggtcta ggaaaatcaa ctctcataaa cagcttattc ctgactgatc
      781 tctacccaga aagagtgatt cctggagctg cagagaaaat tgaaagaact gtccagatag
      841 aggcttcgac tgttgagatt gaagagcggg gtgtgaagct gcggcttaca gtagtggaca
      901 ctcccggcta cggggatgcc atcaactgca gggattgttt caagacaatt atctcctaca
      961 ttgatgagca gtttgaacgc tacctacatg atgagagtgg actgaacagg cgtcacatca
     1021 ttgataacag ggtacattgt tgcttctact tcatttcacc ttttggacat ggactgaagc
     1081 ccttagatgt tgcatttatg aaagcgatac acaataaggt gaatattgtg cctgtcattg
     1141 cgaaagctga cactctcact ctgaaggagc gtgagcggct taagaaaagg attttggatg
     1201 aaattgaaga gcataacatt aaaatctatc acttacctga tgcagagtca gatgaagatg
     1261 aagactttaa ggagcagact agactcctca aggccagtat cccattctct gtggttggct
     1321 ccaaccagtt gattgaagcc aaaggcaaga aggttagagg ccgtctctac ccatggggtg
     1381 ttgtagaggt ggagaaccca gaacacaatg actttctgaa gctgagaacg atgctcatca
     1441 cccacatgca ggacctacag gaagtgaccc aagaccttca ctatgaaaac ttccgttctg
     1501 agaggctgaa gagaggcggc aggaaagtag agaatgagga catgaataaa gaccagatct
     1561 tgcttgaaaa ggaggctgag ctccgccgca tgcaagagat gattgcaaga atgcaagcgc
     1621 agatgcagat gcagatgcag ggtggtgacg gcgacggcgg ggctctcggc caccatgtgg
     1681 tgtccaaggg cgaggagctg ttcaccggcg tggtgcccat cctggtggag ctggatggcg
     1741 acgtgaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgacgcc accaacggca
     1801 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctgg
     1861 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgatcac atgaagcagc
     1921 acgatttctt caagagcgcc atgcccgagg gctacgtgca ggagcgcacc atcagcttca
     1981 aggatgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgat accctggtga
     2041 accgcatcga gctgaagggc atcgatttca aggaggatgg caacatcctg ggccacaagc
     2101 tggagtacaa cttcaacagc cacaacgtgt acatcaccgc cgataagcag aagaacggca
     2161 tcaaggccaa cttcaagatc cgccacaatg tggaggatgg ctccgtgcag ctggccgatc
     2221 actaccagca gaacaccccc atcggcgacg gcccagtgct gctgcccgat aaccactacc
     2281 tgagcaccca gagcaagctg tccaaggacc ccaacgagaa gcgcgatcac atggtgctgc
     2341 tggagttcgt gaccgccgcc ggcatcaccc tgggcatgga tgagctgtac aagtgaggat
     2401 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     2461 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     2521 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     2581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2641 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     2701 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2761 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2821 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2881 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2941 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3001 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3061 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3121 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3181 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3241 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3301 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3361 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3421 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3481 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3541 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     3601 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     3661 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     3721 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     3781 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     3841 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     3901 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     3961 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4021 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4081 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4141 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     4201 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4261 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4321 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4381 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4441 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     4501 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     4561 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     4621 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     4681 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     4741 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     4801 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     4861 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     4921 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4981 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5041 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5101 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5161 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5221 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5281 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5341 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5401 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5461 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5521 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5581 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5641 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5701 cccctgattc tgtggataac cgtattaccg ccatgcat
//
