LOCUS       sequence-358058-        6413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric 
            superfolder GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Kuzmic M, Linares GC, Fialova JL, Iv F, Salaun D, Llewellyn A, Gomes
            M, Belhabib M, Liu Y, Asano K, Rodrigues M, Isnardon D, Tachibana T,
            Koenderink GH, Badache A, Mavrakis M, Verdier-Pinard P
  TITLE     Septin-microtubule association via a motif unique to the isoform 1 
            of septin 9 tunes stress fibers.
  JOURNAL   J Cell Sci. 2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850.
  PUBMED    34854883
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850."
COMMENT     Sequence Label: pCMV_SEPT9_i1 S22A S23A-msfGFP
FEATURES             Location/Qualifiers
     source          1..6413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3201..3322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3238..3257)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3292..3311
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3329..3784)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3466..3487)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3678..3697
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3811..3915
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3917..4274
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3917..4108
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3940..3960)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4125..4260
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4309..5103
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4309..5103
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4363..4382)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4973..4992
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5291..5310)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5335..5382
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5711..6299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6200..6219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatga
      601 agaagtctta ctcaggaggc acgcggacct ccagtggccg gctccggagg cttggtgacg
      661 ccgccggccc agccttgaaa agatcttttg aggtcgagga ggtcgagaca cccaactcca
      721 ccccaccccg gagggtccag actcccctac tccgagccac tgtggccagc tccacccaga
      781 aattccagga cctgggcgtg aagaactcag aaccctcggc ccgccatgtg gactccctaa
      841 gccaacgctc ccccaaggcg tccctgcgga gggtggagct ctcgggcccc aaggcggccg
      901 agccggtgtc ccggcgcact gagctgtcca ttgacatctc gtccaagcag gtggagaacg
      961 ccggggccat cggcccgtcc cggttcgggc tcaagagggc cgaggtgttg ggccacaaga
     1021 cgccagaacc ggcccctcgg aggacggaga tcaccatcgt caaaccccag gagtcagccc
     1081 accggaggat ggagccccct gcctccaagg tccccgaggt gcccactgcc cctgccaccg
     1141 acgcagcccc caagagggtg gagatccaga tgcccaagcc tgctgaggcg cccaccgccc
     1201 ccagcccagc ccagaccttg gagaattcag agcctgcccc tgtgtctcag ctgcagagca
     1261 ggctggagcc caagccccag ccccctgtgg ctgaggctac accccggagc caggaggcca
     1321 ctgaggcggc tcccagctgc gttggcgaca tggccgacac ccccagagat gccgggctca
     1381 agcaggcgcc tgcatcacgg aacgagaagg ccccggtgga cttcggctac gtggggattg
     1441 actccatcct ggagcagatg cgccggaagg ccatgaagca gggcttcgag ttcaacatca
     1501 tggtggtcgg gcagagcggc ttgggtaaat ccaccttaat caacaccctc ttcaaatcca
     1561 aaatcagccg gaagtcggtg cagcccacct cagaggagcg catccccaag accatcgaga
     1621 tcaagtccat cacgcacgat attgaggaga aaggcgtccg gatgaagctg acagtgattg
     1681 acacaccagg gttcggggac cacatcaaca acgagaactg ctggcagccc atcatgaagt
     1741 tcatcaatga ccagtacgag aaatacctgc aggaggaggt caacatcaac cgcaagaagc
     1801 gcatcccgga cacccgcgtc cactgctgcc tctacttcat ccccgccacc ggccactccc
     1861 tcaggcccct ggacatcgag tttatgaaac gcctgagcaa ggtggtcaac atcgtccctg
     1921 tcatcgccaa ggcggacaca ctcaccctgg aggagagggt ccacttcaaa cagcggatca
     1981 ccgcagacct gctgtccaac ggcatcgacg tgtaccccca gaaggaattt gatgaggact
     2041 cggaggaccg gctggtgaac gagaagttcc gggagatgat cccatttgct gtggtgggca
     2101 gtgaccacga gtaccaggtc aacggcaaga ggatccttgg gaggaagacc aagtggggta
     2161 ccatcgaagt tgaaaacacc acacactgtg agtttgccta cctgcgggac cttctcatca
     2221 ggacgcacat gcagaacatc aaggacatca ccagcagcat ccacttcgag gcgtaccgtg
     2281 tgaagcgcct caacgagggc agcagcgcca tggccaacgg cgtggaggag aaggagccag
     2341 aagccccaga gatggtgtcc aagggcgagg agctgttcac cggcgtggtg cccatcctgg
     2401 tggagctgga tggcgacgtg aacggccaca agttcagcgt gcgcggcgag ggcgagggcg
     2461 acgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2521 cctggcccac cctggtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     2581 atcacatgaa gcagcacgat ttcttcaaga gcgccatgcc cgagggctac gtgcaggagc
     2641 gcaccatcag cttcaaggat gacggcacct acaagacccg cgccgaggtg aagttcgagg
     2701 gcgataccct ggtgaaccgc atcgagctga agggcatcga tttcaaggag gatggcaaca
     2761 tcctgggcca caagctggag tacaacttca acagccacaa cgtgtacatc accgccgata
     2821 agcagaagaa cggcatcaag gccaacttca agatccgcca caatgtggag gatggctccg
     2881 tgcagctggc cgatcactac cagcagaaca cccccatcgg cgacggccca gtgctgctgc
     2941 ccgataacca ctacctgagc acccagagca agctgtccaa ggaccccaac gagaagcgcg
     3001 atcacatggt gctgctggag ttcgtgaccg ccgccggcat caccctgggc atggatgagc
     3061 tgtacaagta aggatccacc ggatctagat aactgatcat aatcagccat accacatttg
     3121 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     3181 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     3241 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3301 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
     3361 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3421 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3481 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3541 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3601 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3661 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3721 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     3781 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3841 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3901 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     3961 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4021 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4081 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4141 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4201 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4261 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     4321 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     4381 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     4441 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     4501 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     4561 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     4621 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     4681 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     4741 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     4801 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     4861 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4921 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4981 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     5041 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     5101 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     5161 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     5221 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     5281 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     5341 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     5401 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     5461 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     5521 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     5581 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5821 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6361 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//
