LOCUS       sequence-358059-        6341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human SEPT9_i1 D1-25 fused to monomeric 
            superfolder GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Kuzmic M, Linares GC, Fialova JL, Iv F, Salaun D, Llewellyn A, Gomes
            M, Belhabib M, Liu Y, Asano K, Rodrigues M, Isnardon D, Tachibana T,
            Koenderink GH, Badache A, Mavrakis M, Verdier-Pinard P
  TITLE     Septin-microtubule association via a motif unique to the isoform 1 
            of septin 9 tunes stress fibers.
  JOURNAL   J Cell Sci. 2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850.
  PUBMED    34854883
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2021 Dec 2. pii: 273604. doi: 10.1242/jcs.258850."
COMMENT     Sequence Label: pCMV_SEPT9_i1 D1-25-msfGFP
FEATURES             Location/Qualifiers
     source          1..6341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3129..3250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3166..3185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3220..3239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3257..3712)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3394..3415)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3606..3625
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3739..3843
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3845..4202
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3845..4036
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3868..3888)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4053..4188
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4237..5031
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4237..5031
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4291..4310)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4901..4920
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5219..5238)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5263..5310
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5639..6227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6128..6147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
      601 ccttgaaaag atcttttgag gtcgaggagg tcgagacacc caactccacc ccaccccgga
      661 gggtccagac tcccctactc cgagccactg tggccagctc cacccagaaa ttccaggacc
      721 tgggcgtgaa gaactcagaa ccctcggccc gccatgtgga ctccctaagc caacgctccc
      781 ccaaggcgtc cctgcggagg gtggagctct cgggccccaa ggcggccgag ccggtgtccc
      841 ggcgcactga gctgtccatt gacatctcgt ccaagcaggt ggagaacgcc ggggccatcg
      901 gcccgtcccg gttcgggctc aagagggccg aggtgttggg ccacaagacg ccagaaccgg
      961 cccctcggag gacggagatc accatcgtca aaccccagga gtcagcccac cggaggatgg
     1021 agccccctgc ctccaaggtc cccgaggtgc ccactgcccc tgccaccgac gcagccccca
     1081 agagggtgga gatccagatg cccaagcctg ctgaggcgcc caccgccccc agcccagccc
     1141 agaccttgga gaattcagag cctgcccctg tgtctcagct gcagagcagg ctggagccca
     1201 agccccagcc ccctgtggct gaggctacac cccggagcca ggaggccact gaggcggctc
     1261 ccagctgcgt tggcgacatg gccgacaccc ccagagatgc cgggctcaag caggcgcctg
     1321 catcacggaa cgagaaggcc ccggtggact tcggctacgt ggggattgac tccatcctgg
     1381 agcagatgcg ccggaaggcc atgaagcagg gcttcgagtt caacatcatg gtggtcgggc
     1441 agagcggctt gggtaaatcc accttaatca acaccctctt caaatccaaa atcagccgga
     1501 agtcggtgca gcccacctca gaggagcgca tccccaagac catcgagatc aagtccatca
     1561 cgcacgatat tgaggagaaa ggcgtccgga tgaagctgac agtgattgac acaccagggt
     1621 tcggggacca catcaacaac gagaactgct ggcagcccat catgaagttc atcaatgacc
     1681 agtacgagaa atacctgcag gaggaggtca acatcaaccg caagaagcgc atcccggaca
     1741 cccgcgtcca ctgctgcctc tacttcatcc ccgccaccgg ccactccctc aggcccctgg
     1801 acatcgagtt tatgaaacgc ctgagcaagg tggtcaacat cgtccctgtc atcgccaagg
     1861 cggacacact caccctggag gagagggtcc acttcaaaca gcggatcacc gcagacctgc
     1921 tgtccaacgg catcgacgtg tacccccaga aggaatttga tgaggactcg gaggaccggc
     1981 tggtgaacga gaagttccgg gagatgatcc catttgctgt ggtgggcagt gaccacgagt
     2041 accaggtcaa cggcaagagg atccttggga ggaagaccaa gtggggtacc atcgaagttg
     2101 aaaacaccac acactgtgag tttgcctacc tgcgggacct tctcatcagg acgcacatgc
     2161 agaacatcaa ggacatcacc agcagcatcc acttcgaggc gtaccgtgtg aagcgcctca
     2221 acgagggcag cagcgccatg gccaacggcg tggaggagaa ggagccagaa gccccagaga
     2281 tggtgtccaa gggcgaggag ctgttcaccg gcgtggtgcc catcctggtg gagctggatg
     2341 gcgacgtgaa cggccacaag ttcagcgtgc gcggcgaggg cgagggcgac gccaccaacg
     2401 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2461 tggtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgat cacatgaagc
     2521 agcacgattt cttcaagagc gccatgcccg agggctacgt gcaggagcgc accatcagct
     2581 tcaaggatga cggcacctac aagacccgcg ccgaggtgaa gttcgagggc gataccctgg
     2641 tgaaccgcat cgagctgaag ggcatcgatt tcaaggagga tggcaacatc ctgggccaca
     2701 agctggagta caacttcaac agccacaacg tgtacatcac cgccgataag cagaagaacg
     2761 gcatcaaggc caacttcaag atccgccaca atgtggagga tggctccgtg cagctggccg
     2821 atcactacca gcagaacacc cccatcggcg acggcccagt gctgctgccc gataaccact
     2881 acctgagcac ccagagcaag ctgtccaagg accccaacga gaagcgcgat cacatggtgc
     2941 tgctggagtt cgtgaccgcc gccggcatca ccctgggcat ggatgagctg tacaagtaag
     3001 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
     3061 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     3121 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3241 atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     3301 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     3361 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     3421 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     3481 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     3541 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     3601 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     3661 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     3721 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3781 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3841 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3901 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3961 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4021 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4081 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4201 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     4261 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     4321 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     4381 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     4441 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     4501 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     4561 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     4621 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     4681 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     4741 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     4801 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     4861 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     4921 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     4981 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     5041 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     5101 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     5161 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     5221 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     5281 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     5341 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     5401 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     5461 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     5521 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5581 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5641 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5701 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5761 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5821 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5881 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5941 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6001 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6061 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6301 tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
